본문 바로가기
HOME> 저널/프로시딩 > 저널/프로시딩 검색상세

저널/프로시딩 상세정보

권호별목차 / 소장처보기

H : 소장처정보

T : 목차정보

Biotechnology and bioengineering 22건

  1. [해외논문]   Cover Image, Volume 115, Number 8, August 2018   SCI SCIE

    Liu, Zhuolin (Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona ) , Zhou, Chen (Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona ) , Ontiveros‐ (Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona ) , Valencia, Aura (Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona ) , Luo, Yi‐ (Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona ) , Hao (Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona ) , Long, Min (Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, Arizona) , Xu, Hua , Rittmann, Bruce E.
    Biotechnology and bioengineering v.115 no.8 ,pp. i - i , 2018 , 0006-3592 ,

    초록

    The cover image, by Zhuolin Liu et al., is based on the Article Accurate O2 delivery enabled benzene biodegradation through aerobic activation followed by denitrification‐coupled mineralization , DOI: 10.1002/bit.26712 .

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  2. [해외논문]   Biotechnology and Bioengineering: Volume 115, Number 8, August 2018   SCI SCIE


    Biotechnology and bioengineering v.115 no.8 ,pp. 1885 - 1889 , 2018 , 0006-3592 ,

    초록

    The cover image, by Zhuolin Liu et al., is based on the Article Accurate O2 delivery enabled benzene biodegradation through aerobic activation followed by denitrification‐coupled mineralization , DOI: 10.1002/bit.26712 .

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  3. [해외논문]   Mechanisms driving the lactate switch in Chinese hamster ovary cells   SCI SCIE

    Hartley, Fiona (University of Oxford, Oxford, Oxfordshire, UK) , Walker, Tracy (GlaxoSmithKline, Stevenage, Hertfordshire, UK) , Chung, Vicky (GlaxoSmithKline, Stevenage, Hertfordshire, UK) , Morten, Karl (University of Oxford, Oxford, Oxfordshire, UK)
    Biotechnology and bioengineering v.115 no.8 ,pp. 1890 - 1903 , 2018 , 0006-3592 ,

    초록

    Abstract The metabolism of Chinese Hamster Ovary (CHO) cells in a production environment has been extensively investigated. However, a key metabolic transition, the switch from lactate production to lactate consumption, remains enigmatic. Though commonly observed in CHO cultures, the mechanism(s) by which this metabolic shift is triggered is unknown. Despite this, efforts to control the switch have emerged due to the association of lactate consumption with improved cell growth and productivity. This review aims to consolidate current theories surrounding the lactate switch. The influence of pH, NAD + /NADH, pyruvate availability and mitochondrial function on lactate consumption are explored. A hypothesis based on the cellular redox state is put forward to explain the onset of lactate consumption. Various techniques implemented to control the lactate switch, including manipulation of the culture environment, genetic engineering, and cell line selection are also discussed.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  4. [해외논문]   Unexpected instabilities explain batch‐to‐batch variability in cell‐free protein expression systems   SCI SCIE

    Hunter, Dominic J.B. (EMBL Australia Node for Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Australia) , Bhumkar, Akshay (EMBL Australia Node for Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Australia) , Giles, Nichole (EMBL Australia Node for Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Australia) , Sierecki, Emma (EMBL Australia Node for Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Australia) , Gambin, Yann (EMBL Australia Node for Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Australia)
    Biotechnology and bioengineering v.115 no.8 ,pp. 1904 - 1914 , 2018 , 0006-3592 ,

    초록

    Abstract Cell‐free methods of protein synthesis offer rapid access to expressed proteins. Though the amounts produced are generally only at a small scale, these are sufficient to perform protein‐protein interaction assays and tests of enzymatic activity. As such they are valuable tools for the biochemistry and bioengineering community. However the most complex, eukaryotic cell‐free systems are difficult to manufacture in house and can be prohibitively expensive to obtain from commercial sources. The Leishmania tarentolae system offers a relatively cheap alternative which is capable of producing difficult to express proteins, but which is simpler to produce in large scale. However, this system suffers from batch‐to‐batch variability, which has been accepted as a consequence of the complexity of the extracts. Here we show an unexpected origin for the variability observed and demonstrate that small variations in a single parameter can dramatically affect expression, such that minor pipetting errors can have major effects on yields. L. tarentolae cell‐free lysate activity is shown to be more stable to changes in Mg 2+ concentration at a lower ratio of feed solution to lysate in the reaction than typically used, and a higher Mg 2+ optimum. These changes essentially eliminate batch‐to‐batch variability of L. tarentolae lysate activity and permit their full potential to be realized.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  5. [해외논문]   Advances in industrial biopharmaceutical batch process monitoring: Machine‐learning methods for small data problems   SCI SCIE

    Tulsyan, Aditya (Digital Integration and Predictive Technologies, Amgen, Inc., Cambridge, Massachusetts ) , Garvin, Christopher (Digital Integration and Predictive Technologies, Amgen, Inc., West Greenwich, Rhode Island ) , Ü (Digital Integration and Predictive Technologies, Amgen, Inc., Thousand Oaks, California) , ndey, Cenk
    Biotechnology and bioengineering v.115 no.8 ,pp. 1915 - 1924 , 2018 , 0006-3592 ,

    초록

    Abstract Biopharmaceutical manufacturing comprises of multiple distinct processing steps that require effective and efficient monitoring of many variables simultaneously in real‐time. The state‐of‐the‐art real‐time multivariate statistical batch process monitoring (BPM) platforms have been in use in recent years to ensure comprehensive monitoring is in place as a complementary tool for continued process verification to detect weak signals. This article addresses a longstanding, industry‐wide problem in BPM, referred to as the “Low‐N” problem, wherein a product has a limited production history. The current best industrial practice to address the Low‐N problem is to switch from a multivariate to a univariate BPM, until sufficient product history is available to build and deploy a multivariate BPM platform. Every batch run without a robust multivariate BPM platform poses risk of not detecting potential weak signals developing in the process that might have an impact on process and product performance. In this article, we propose an approach to solve the Low‐N problem by generating an arbitrarily large number of in silico batches through a combination of hardware exploitation and machine‐learning methods. To the best of authors’ knowledge, this is the first article to provide a solution to the Low‐N problem in biopharmaceutical manufacturing using machine‐learning methods. Several industrial case studies from bulk drug substance manufacturing are presented to demonstrate the efficacy of the proposed approach for BPM under various Low‐N scenarios.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  6. [해외논문]   Pro region engineering of nerve growth factor by deep mutational scanning enables a yeast platform for conformational epitope mapping of anti‐NGF monoclonal antibodies   SCI SCIE

    Medina‐ (Department of Chemical Engineering and Materials Science, Michigan State University Engineering Building, East Lansing, Michigan ) , Cucurella, Angé (Zoetis Global Therapeutic Research, Veterinary Medicine Research and Development, Kalamazoo, Michigan ) , lica V. (Zoetis Global Therapeutic Research, Veterinary Medicine Research and Development, Kalamazoo, Michigan ) , Zhu, Yaqi (Zoetis Global Therapeutic Research, Veterinary Medicine Research and Development, Kalamazoo, Michigan ) , Bowen, Scott J. (Department of Chemical Engineering and Materials Science, Michigan State University Engineering Building, East Lansing, Michigan) , Bergeron, Lisa M. , Whitehead, Timothy A.
    Biotechnology and bioengineering v.115 no.8 ,pp. 1925 - 1937 , 2018 , 0006-3592 ,

    초록

    Abstract Nerve growth factor (NGF) plays a central role in multiple chronic pain conditions. As such, anti‐NGF monoclonal antibodies (mAbs) that function by antagonizing NGF downstream signaling are leading drug candidates for non‐opioid pain relief. To evaluate anti‐canine NGF (cNGF) mAbs we sought a yeast surface display platform of cNGF. Both mature cNGF and pro‐cNGF displayed on the yeast surface but bound conformationally sensitive mAbs at most 2.5‐fold in mean fluorescence intensity above background, suggesting that cNGF was mostly misfolded. To improve the amount of folded, displayed cNGF, we used comprehensive mutagenesis, FACS, and deep sequencing to identify point mutants in the pro‐region of canine NGF that properly enhance the folded protein displayed on the yeast surface. Out of 1,737 tested single point mutants in the pro region, 49 increased the amount of NGF recognized by conformationally sensitive mAbs. These gain‐of‐function mutations cluster around residues A‐61–P‐26. Gain‐of‐function mutants were additive, and a construct containing three mutations increased amount of folded cNGF to 23‐fold above background. Using this new cNGF construct, fine conformational epitopes for tanezumab and three anti‐cNGF mAbs were evaluated. The epitope revealed by the yeast experiments largely overlapped with the tanezumab epitope previously determined by X‐ray crystallography. The other mAbs showed site‐specific differences with tanezumab. As the number of binding epitopes of functionally neutralizing anti‐NGF mAbs on NGF are limited, subtle differences in the individual interacting residues on NGF that bind each mAb contribute to the understanding of each antibody and variations in its neutralizing activity. These results demonstrate the potential of deep sequencing‐guided protein engineering to improve the production of folded surface‐displayed protein, and the resulting cNGF construct provides a platform to map conformational epitopes for other anti‐neurotrophin mAbs.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  7. [해외논문]   Contributions of depth filter components to protein adsorption in bioprocessing   SCI SCIE

    Khanal, Ohnmar (Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware ) , Singh, Nripen (Biologics Process Development, Global Product Development and Supply, Bristol‐Myers Squibb Company, Devens, Massachusetts ) , Traylor, Steven J. (Biologics Process Development, Global Product Development and Supply, Bristol‐Myers Squibb Company, Devens, Massachusetts ) , Xu, Xuankuo (Biologics Process Development, Global Product Development and Supply, Bristol‐Myers Squibb Company, Devens, Massachusetts ) , Ghose, Sanchayita (Biologics Process Development, Global Product Development and Supply, Bristol‐Myers Squibb Company, Devens, Massachusetts ) , Li, Zheng J. (Biologics Process Development, Global Product Development and Supply, Bristol‐Myers Squibb Company, Devens, Massachusetts ) , Lenhoff, Abraham M. (Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware)
    Biotechnology and bioengineering v.115 no.8 ,pp. 1938 - 1948 , 2018 , 0006-3592 ,

    초록

    Abstract Depth filtration is widely used in downstream bioprocessing to remove particulate contaminants via depth straining and is therefore applied to harvest clarification and other processing steps. However, depth filtration also removes proteins via adsorption, which can contribute variously to impurity clearance and to reduction in product yield. The adsorption may occur on the different components of the depth filter, that is, filter aid, binder, and cellulose filter. We measured adsorption of several model proteins and therapeutic proteins onto filter aids, cellulose, and commercial depth filters at pH 5–8 and ionic strengths

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  8. [해외논문]   Single pass diafiltration integrated into a fully continuous mAb purification process   SCI SCIE

    Rucker‐ (Department of Bioprocess Engineering, MedImmune, Gaithersburg, Maryland ) , Pezzini, Joanna (Department of Bioprocess Engineering, MedImmune, Gaithersburg, Maryland ) , Arnold, Lindsay (Department of Bioprocess Engineering, MedImmune, Gaithersburg, Maryland ) , Hill‐ (Department of Bioprocess Engineering, MedImmune, Gaithersburg, Maryland ) , Byrne, Kevin (Department of Bioprocess Engineering, MedImmune, Gaithersburg, Maryland ) , Sharp, Tom (Department of Bioprocess Engineering, MedImmune, Gaithersburg, Maryland) , Avazhanskiy, Maksim , Forespring, Chris
    Biotechnology and bioengineering v.115 no.8 ,pp. 1949 - 1957 , 2018 , 0006-3592 ,

    초록

    Abstract The concept of continuous manufacturing has gained significant interest from the biopharmaceutical industry over the past several years. Benefits include increased manufacturing productivity, improved quality control, reduction in plant footprint, and more flexible management of facility capacity. There are several technologies currently available that enable continuous processing for chromatography and ultrafiltration. However, a single pass diafiltration design that meets the required small molecule clearance and has been integrated into a fully continuous monoclonal antibody purification process has not been previously published. Here, the theory and design of a 3‐stage single pass diafiltration step is presented. Buffer exchange greater than 99.75% was experimentally demonstrated. Several critical design aspects were incorporated to minimize system complexity and reduce the buffer volume requirements. Lastly, single pass diafiltration was demonstrated in a pilot scale continuous process with uninterrupted flow from the bioreactor through the formulation step. This work illustrates the feasibility of incorporating a single pass diafiltration step into an end‐to‐end continuous protein purification process.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  9. [해외논문]   Quantitatively characterizing drug‐induced arrhythmic contractile motions of human stem cell‐derived cardiomyocytes   SCI SCIE

    Hoang, Plansky (Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, New York ) , Huebsch, Nathaniel (Department of Bioengineering, University of California, Berkeley, California ) , Bang, Shin Hyuk (Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, New York ) , Siemons, Brian A. (Department of Bioengineering, University of California, Berkeley, California ) , Conklin, Bruce R. (Glastone Institute of Cardiovascular Diseases, San Francisco, California ) , Healy, Kevin E. (Department of Bioengineering, University of California, Berkeley, California ) , Ma, Zhen (Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, New York ) , Jacquir, Sabir (Laboratoire LE2I UMR CNRS 6306, Université)
    Biotechnology and bioengineering v.115 no.8 ,pp. 1958 - 1970 , 2018 , 0006-3592 ,

    초록

    Abstract Quantification of abnormal contractile motions of cardiac tissue has been a noteworthy challenge and significant limitation in assessing and classifying the drug‐induced arrhythmias (i.e., Torsades de pointes). To overcome these challenges, researchers have taken advantage of computational image processing tools to measure contractile motion from cardiomyocytes derived from human induced pluripotent stem cells (hiPSC‐CMs). However, the amplitude and frequency analysis of contractile motion waveforms does not produce sufficient information to objectively classify the degree of variations between two or more sets of cardiac contractile motions. In this paper, we generated contractile motion data from beating hiPSC‐CMs using motion tracking software based on optical flow analysis, and then implemented a computational algorithm, phase space reconstruction (PSR), to derive parameters (embedding, regularity, and fractal dimensions) to further characterize the dynamic nature of the cardiac contractile motions. Application of drugs known to cause cardiac arrhythmia induced significant changes to these resultant dimensional parameters calculated from PSR analysis. Integrating this new computational algorithm with the existing analytical toolbox of cardiac contractile motions will allow us to expand current assessments of cardiac tissue physiology into an automated, high‐throughput, and quantifiable manner which will allow more objective assessments of drug‐induced proarrhythmias.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  10. [해외논문]   Enhanced isobutanol production from acetate by combinatorial overexpression of acetyl‐CoA synthetase and anaplerotic enzymes in engineered Escherichia coli   SCI SCIE

    Song, Hun‐ (Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Korea) , Suk (Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Korea) , Seo, Hyung‐ (Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Korea) , Min (Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Korea) , Jeon, Jong‐ (Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Korea) , Min (Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Korea) , Moon, Yu‐ (Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, Korea) , Mi (Biotechnology Process Engineering Center, Korea Research Insti) , Hong, Ju Won , Hong, Yoon Gi , Bhatia, Shashi Kant , Ahn, Jungoh , Lee, Hongweon , Kim, Wooseong , Park, Yong‐ , Cheol , Choi, Kwon‐ , Young , Kim, Yun‐ , Gon , Yang, Yung‐ , Hun
    Biotechnology and bioengineering v.115 no.8 ,pp. 1971 - 1978 , 2018 , 0006-3592 ,

    초록

    Acetic acid is an abundant material that can be used as a carbon source by microorganisms. Despite its abundance, its toxicity and low energy content make it hard to utilize as a sole carbon source for biochemical production. To increase acetate utilization and isobutanol production with engineered Escherichia coli, the feasibility of utilizing acetate and metabolic engineering was investigated. The expression of acs, pckA, and maeB increased isobutanol production by up to 26%, and the addition of TCA cycle intermediates indicated that the intermediates can enhance isobutanol production. For isobutanol production from acetate, acetate uptake rates and the NADPH pool were not limiting factors compared to glucose as a carbon source. This work represents the first approach to produce isobutanol from acetate with pyruvate flux optimization to extend the applicability of acetate. This technique suggests a strategy for biochemical production utilizing acetate as the sole carbon source.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지

논문관련 이미지