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Molecular and cellular neurosciences 23건

  1. [해외논문]   Cover 2   SCI SCIE


    Molecular and cellular neurosciences v.85 ,pp. IFC - IFC , 2017 , 1044-7431 ,

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  2. [해외논문]   Alpha-synuclein ferrireductase activity is detectible in vivo, is altered in Parkinson's disease and increases the neurotoxicity of DOPAL   SCI SCIE

    McDowall, Jennifer S. (Department of Biology and Biochemistry, University of Bath, Bath, UK ) , Ntai, Ioanna (Department of Biology and Biochemistry, University of Bath, Bath, UK ) , Honeychurch, Kevin C. (Department of Biological, Biomedical and Analytical Sciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, UK ) , Hart, John P. (Department of Biological, Biomedical and Analytical Sciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, UK ) , Colin, Philippe (Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne 1015, Switzerland ) , Schneider, Bernard L. (Brain Mind Institute, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne 1015, Switzerland ) , Brown, David R. (Department of Biology and Biochemistry, University of Bath, Bath, UK)
    Molecular and cellular neurosciences v.85 ,pp. 1 - 11 , 2017 , 1044-7431 ,

    초록

    Abstract The normal cellular role of α-synuclein is of potential importance in understanding diseases in which an aggregated form of the protein has been implicated. A potential loss or change in the normal function of α-synuclein could play a role in the aetiology of diseases such as Parkinson's disease. Recently, it has been suggested that α-synuclein could cause the enzymatic reduction of iron and a cellular increase in Fe(II) levels. Experiments were carried out to determine if such activity could be measured in vivo . Experiments with rats overexpressing human α-synuclein in nigral dopaminergic neurons demonstrated a correlation between α-synuclein expression and ferrireductase activity. Furthermore, studies on tissue from Parkinson's disease patient brains showed a significant decrease in ferrireductase activity, possibly due to deposition of large amounts of inactive protein. Cellular studies suggest that increase ferrireductase activity results in increased levels of dopamine metabolites and increased sensitivity to the toxicity of DOPAL. These findings demonstrate that α-synuclein ferrireductase activity is present in vivo and its alteration may play a role in neuron loss in disease. Highlights Alpha-synuclein is a ferrireductase in an in vivo rat model. Parkinson's disease patients show changes in ferrireductase activity in the striatum. Increased ferrireductase activity increases HVA and DOPAC levels. Increased ferrireductase causes increases sensitivity to DOPAL toxicity.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  3. [해외논문]   The actin binding protein scinderin acts in PC12 cells to tether dense-core vesicles prior to secretion   SCI SCIE

    Wang, J. (Department of Anesthesia, Cincinnati Children's Hospital Medical Center, MLC2001, 3333 Burnet Avenue, Cincinnati, OH 45229, United States ) , Richards, D.A. (Department of Anesthesia, Cincinnati Children's Hospital Medical Center, MLC2001, 3333 Burnet Avenue, Cincinnati, OH 45229, United States)
    Molecular and cellular neurosciences v.85 ,pp. 12 - 18 , 2017 , 1044-7431 ,

    초록

    Abstract Mechanistic understanding of the control of vesicle motion from within a secretory cell to the site of exocytosis remains incomplete. In this work, we have used total internal reflection (TIRF) microscopy to examine the mobility of secretory vesicles at the plasma membrane. Under resting conditions, we found vesicles showed little lateral mobility. Anchoring of vesicles in this membrane proximal compartment could be disrupted with latrunculin A, indicating an apparent actin dependent process. A candidate intermediary between vesicles and the actin skeleton is the actin binding protein scinderin. Co-transfection of an shRNA construct against scinderin blocked secretion, and also increased the mobility of vesicles in the membrane-proximal section of the cell, indicating a dual role for scinderin in secretion; tethering vesicles to the cytoskeleton, as well as liberating them following stimulation through the previously described calcium dependent actin severing activity. Analysis of lipid dependence indicates that scinderin exhibits calcium dependent binding to phosphatidyl-inositol monophosphate, providing a possible mechanism for vesicle binding. Highlights Scinderin is required for dense-core vesicle exocytosis in PC12 cells. In resting cells, scinderin acts to prevent vesicle motion. Scinderin exhibits bell-shaped dependence of calcium binding to mono-phosphorylated forms of phosphatidyl inositol. Loss of lipid binding domains prevents scinderin's function in secretion.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  4. [해외논문]   The expression of pluripotency and neuronal differentiation markers under the influence of electromagnetic field and nitric oxide   SCI SCIE

    Haghighat, Nazanin (Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran ) , Abdolmaleki, Parviz (Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran ) , Parnian, Javad (Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran ) , Behmanesh, Mehrdad (Department of Genetics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran)
    Molecular and cellular neurosciences v.85 ,pp. 19 - 28 , 2017 , 1044-7431 ,

    초록

    Abstract Nitric oxide (NO) is a diatomic free radical compound that as a secondary messenger contributes to cell physiological functions and its variations influence proteins activity and triggering intracellular signaling cascades. Low frequency electromagnetic field (EMF) alters the cell biology such as cell differentiation by targeting the plasma membrane and entering force to the ions and small electrical ligands. The effect of these chemical (NO) and physical (EMF) factors on the expression of the stemness and neuronal differentiation markers in rat bone marrow mesenchymal stem cells (BMSC) was investigated. The cells were treated with low (50micromolar) and high (1mM) concentrations of Deta-NO as a NO donor molecule and 50Hz low frequency EMF. The expression of pluripotency and neuronal differentiation genes and proteins was investigated using real time qPCR and Immunocytochemistry techniques. The simultaneous treatment of EMF with NO (1mM) led to the down-regulation of stemness markers expression and up-regulation of neuronal differentiation markers expression. Cell proliferation decreased and cell morphology changed which caused the majority of cells obtains neuronal protein markers in their cytoplasm. The decrease in the expression of neuronal differentiation Nestin and DCX markers without any change in the expression of pluripotency Oct4 marker (treated with low concentration of NO) indicates protection of stemness state in these cells. Treatment with NO demonstrated a double behavior. NO low concentration helped the cells protect the stemness state but NO high concentration plus EMF pushed cells into differentiation pathway. Highlights BMSCs have different behaviors in low and high concentrations of NO. Low concentration of NO helped to protect of pluripotency state of BMSCs. High concentration of NO plus electromagnetic field led to express the neuronal differentiation markers in more cells. EMF helped polarization of cells so more cells achieved morphology like neurons. Differentiation of BMSCs into neuronal cells was facilitated by simultaneous treatment of EMF and NO (1mM).

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Cocaine modifies brain lipidome in mice   SCI SCIE

    Lin, Yiyun (National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy, Chengdu 610041, China ) , Gu, Hui (National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy, Chengdu 610041, China ) , Jiang, Linhong (National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy, Chengdu 610041, China ) , Xu, Wei (National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy, Chengdu 610041, China ) , Liu, Chunqi (State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative) , Li, Yan , Qian, Xinying , Li, Dandan , Li, Zhuoling , Hu, Jing , Zhang, Huaqin , Guo, Wei , Zhao, Yinglan , Cen, Xiaobo
    Molecular and cellular neurosciences v.85 ,pp. 29 - 44 , 2017 , 1044-7431 ,

    초록

    Abstract Lipids are predominant components of the brain and key regulators for neural structure and function. The neuropsychopharmacological effect of cocaine has been intensively investigated; however, the impact of cocaine on brain lipid profiles is largely unknown. In this study, we used a LC-MS-based lipidomic approach to investigate the impact of cocaine on brain lipidome in two mouse models, cocaine-conditioned place preference (CPP) and hyperlocomotor models and the lipidome was profoundly modified in the nucleus accumbens (NAc) and striatum respectively. We comprehensively analyzed the lipids among 21 subclasses across 7 lipid classes and found that cocaine profoundly modified brain lipidome. Notably, the lipid metabolites significantly modified were sphingolipids and glycerophospholipids in the NAc, showing a decrease in ceramide and an increase in its up/downstream metabolites levels, and decrease lysophosphatidylcholine (LPC) and lysophosphoethanolamine (LPE) and increase phosphatidylcholine (PC) and phosphatidylethanolamines (PE) levels, respectively. Moreover, long and polyunsaturated fatty acid phospholipids were also markedly increased in the NAc. Our results show that cocaine can markedly modify brain lipidomic profiling. These findings reveal a link between the modified lipidome and psychopharmacological effect of cocaine, providing a new insight into the mechanism of cocaine addiction. Highlights Cocaine intensively modified lipidome in NAc. Cocaine mainly affected phospholipid and sphingolipid metabolism. Ceramide was decreased in NAc after cocaine administration. Cocaine increased long and polyunsaturated fatty acid phospholipids in NAc.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [해외논문]   TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway   SCI SCIE

    Li, Yijian (Corresponding authors at: Southwest Hospital/Southwest Eye Hospital, Third Military Medical University, Chongqing, China. ) , Zou, Ting (Corresponding authors at: Southwest Hospital/Southwest Eye Hospital, Third Military Medical University, Chongqing, China.) , Xue, Langyue , Yin, Zheng Qin , Huo, Shujia , Xu, Haiwei
    Molecular and cellular neurosciences v.85 ,pp. 45 - 56 , 2017 , 1044-7431 ,

    초록

    Abstract Olfactory ensheathing cells (OECs) have been shown to be a leading candidate in cell therapies for central nervous system (CNS) injuries and neurodegenerative diseases. Rapid clearance of neuron debris can promote neuronal survival and axonal regeneration in CNS injuries and neurodegenerative diseases. The phagocytic removal of neuron debris by OECs has been shown to contribute to neuronal outgrowth. However, the precise molecular and cellular mechanisms of phagocytic removal of neuron debris by OECs have not been explored. In this study, we found that OECs secreted anti-inflammatory cytokine transforming growth factor β1 (TGF-β1) during the phagocytic removal of neuron debris. TGF-β1 enhanced phagocytic activity of OECs through regulating integrin/MFG-E8 signaling pathway. In addition, TGF-β1 shifted OECs towards a flattened shape with increased cellular area, which might also be involved in the enhancement of phagocytic activity of OECs. Furthermore, the removal of neuron debris by OECs affected neuronal survival and outgrowth. TGF-β1 enhanced the clearance of neuron debris by OECs and increased neuronal survival. These results reveal the role and mechanism of TGF-β1 in enhancing the phagocytic activity of OECs, which will update the understanding of phagocytosis of OECs and improve the therapeutic use of OECs in CNS injuries and neurodegenerative diseases. Highlights OECs secreted TGF-β1 during the phagocytic removal of neuron debris. TGF-β1 enhanced phagocytic activity of OECs through regulating integrin/MFG-E8 pathway. TGF-β1 enhanced the clearance of neuron debris by OECs and increased neuronal survival. TGF-β1 shifted OECs towards a flattened shape.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [해외논문]   NeuronRead, an open source semi-automated tool for morphometric analysis of phase contrast and fluorescence neuronal images   SCI SCIE

    Dias, Roberto A. (Cell Differentiation and Regeneration group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, Universidade de Aveiro, Aveiro, Portugal ) , Gonç (Cell Differentiation and Regeneration group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, Universidade de Aveiro, Aveiro, Portugal ) , alves, Bruno P. (Cell Differentiation and Regeneration group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, Universidade de Aveiro, Aveiro, Portugal ) , da Rocha, Joana F. (Neurosciences and Signalling group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, Universidade de Aveiro, Aveiro, Portugal ) , da Cruz e Silva, Odete A.B. (Instituto de Engenharia Electrónica e Telemática (IEETA), Departamento de Electrónica e Telecomunicações (DETI), Universidade de Aveiro, Aveiro, Portugal ) , da Silva, Augusto M.F. (Cell Differentiation and Regeneration group, Institute of Biomedicine (iBiMED), Department of Medical Sciences, Universidade de Aveiro, Aveiro, Portugal) , Vieira, Sandra I.
    Molecular and cellular neurosciences v.85 ,pp. 57 - 69 , 2017 , 1044-7431 ,

    초록

    Abstract Neurons are specialized cells of the Central Nervous System whose function is intricately related to the neuritic network they develop to transmit information. Morphological evaluation of this network and other neuronal structures is required to establish relationships between neuronal morphology and function, and may allow monitoring physiological and pathophysiologic alterations. Fluorescence-based microphotographs are the most widely used in cellular bioimaging, but phase contrast (PhC) microphotographs are easier to obtain, more affordable, and do not require invasive, complicated and disruptive techniques. Despite the various freeware tools available for fluorescence-based images analysis, few exist that can tackle the more elusive and harder-to-analyze PhC images. To surpass this, an interactive semi-automated image processing workflow was developed to easily extract relevant information (e.g. total neuritic length, average cell body area) from both PhC and fluorescence neuronal images. This workflow, named ‘NeuronRead’, was developed in the form of an ImageJ macro. Its robustness and adaptability were tested and validated on rat cortical primary neurons under control and differentiation inhibitory conditions. Validation included a comparison to manual determinations and to a golden standard freeware tool for fluorescence image analysis. NeuronRead was subsequently applied to PhC images of neurons at distinct differentiation days and exposed or not to DAPT, a pharmacological inhibitor of the γ-secretase enzyme, which cleaves the well-known Alzheimer's amyloid precursor protein (APP) and the Notch receptor. Data obtained confirms a neuritogenic regulatory role for γ-secretase products and validates NeuronRead as a time- and cost-effective useful monitoring tool. Highlights Novel semi-automatic easy-to-use macro for morphological analysis of neuronal cells. The NeuronRead macro can analyze both Phase Contrast and Fluorescence 2D images. NeuronRead extracts morphometric data from neuronal differentiation cell models. NeuronRead is able to detect morphological changes induced by drugs such as DAPT.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [해외논문]   A subpopulation of activated retinal macrophages selectively migrated to regions of cone photoreceptor stress, but had limited effect on cone death in a mouse model for type 2 Leber congenital amaurosis   SCI SCIE

    Tang, Peter H. (Corresponding author at: Dept. Ophthalmology & Visual Neurosciences, University of Minnesota, Lions Res. Bldg. Rm 314, 2001 6th St SE, Minneapolis, MN 55455, United States.) , Pierson, Mark J. , Heuss, Neal D. , Gregerson, Dale S.
    Molecular and cellular neurosciences v.85 ,pp. 70 - 81 , 2017 , 1044-7431 ,

    초록

    Abstract Background Studies of antigen presentation in retina using mice that expressed green fluorescent protein (GFP) from a transgenic CD11c promoter found that retinal GFP hi cells possessed antigen presentation function. Subsequent studies found that these high GFP hi cells preferentially localized to sites of retinal injury, consistent with their APC function. Interest in the roles of macrophages in degenerative CNS diseases led us to study the GFP hi cells in a retinal model of neurodegeneration. We asked if apoptotic cone photoreceptor cell death in Rpe65 −/− knockout mice induced the GFP hi cells, explored their relationship to resident microglia (MG), and tested their role in cone survival. Methods Rpe65 −/− mice were bred to CD11c GFP mice on the B6/J background. CD11c GFP Rpe65 −/− mice were also backcrossed to CX3CR1 YFP-creER ROSA DTA mice so that CX3CR1 + mononuclear cells could be depleted by Tamoxifen. Retinas were analyzed by immunohistochemistry, confocal microscopy, fluorescence fundoscopy and flow cytometry. Results Elevated numbers of GFP hi cells were concentrated in photoreceptor cell layers of CD11c GFP Rpe65 −/− mice coinciding with the peak of cone death at 2 to 4weeks of age, and persisted for at least 14months. After the initial wave of cone loss, a slow progressive loss of cones was found that continued to retain GFP hi cells in the outer retina. Sustained, four-week Tamoxifen depletions of the GFP hi cells and MG in Rpe65 −/− mice from day 13 to day 41, and from day 390 to day 420 promoted a small increase in cone survival. We found no evidence that the GFP hi cells were recruited from the circulation; all data pointed to a MG origin. MG and GFP hi cells were well segregated in the dystrophic retina; GFP hi cells were foremost in the photoreceptor cell layer, while MG were concentrated in the inner retina. Conclusions The expression of GFP on a subset of retinal mononuclear cells in CD11c GFP mice identified a distinct population of cells performing functions previously attributed to MG. Although GFP hi cells dominated the macrophage response to cone death in the photoreceptor cell layer, their ablation led to only an incremental increase in cone survival. The ability to identify, ablate, and isolate these cells will facilitate analysis of this activated, antigen-presenting subset of MG. Highlights Retinal macrophages expressing GFP in CD11c GFP mice concentrated in the photoreceptor cell layer of RPE65 knockout mice. Elevated numbers of GFP hi cells remained in the outer retinas of knockout mice for more than a year. Generation of the GFP hi cells was found to be dependent on microglia. Tamoxifen-induced macrophage ablation gave a small increase in S-cone survival in acute and chronic phases of cone loss. GFP expression on retinal mononuclear cells in CD11c GFP mice identified cells performing functions of activated microglia.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  9. [해외논문]   Attentional deficits and altered neuronal activation in medial prefrontal and posterior parietal cortices in mice with reduced dopamine transporter levels   SCI SCIE

    Cybulska-Klosowicz, Anita (Nencki Institute of Experimental Biology of Polish Academy of Sciences, PAS, 02-093 Warsaw, Poland ) , Laczkowska, Marta (Warsaw University of Technology, 00-664 Warsaw, Poland ) , Zakrzewska, Renata (Nencki Institute of Experimental Biology of Polish Academy of Sciences, PAS, 02-093 Warsaw, Poland ) , Kaliszewska, Aleksandra (Nencki Institute of Experimental Biology of Polish Academy of Sciences, PAS, 02-093 Warsaw, Poland)
    Molecular and cellular neurosciences v.85 ,pp. 82 - 92 , 2017 , 1044-7431 ,

    초록

    Abstract The executive control function of attention is regulated by the dopaminergic (DA) system. Dopamine transporter (DAT) likely plays a role in controlling the influence of DA on cognitive processes. We examined the effects of DAT depletion on cognitive processes related to attention. Mice with the DAT gene genetically deleted (DAT+/− heterozygotes) were compared to wild type (WT) mice on the Attentional Set-Shifting Task (ASST). Changes in neuronal activity during the ASST were shown with early growth response genes 1 and 2 (egr-1 and egr-2) immunohistochemistry in the medial prefrontal cortex (mPFC) and in the posterior parietal cortex (PPC). Heterozygotes were impaired in tasks that tax reversal learning, attentional-set formation and set-shifting. Densities of egr-2 labeled cells in the mPFC were lower in mutant mice when compared with wild-types in intradimensional shift of attention (IDS), extradimensional shift of attention and extradimensional shift of attention-reversal phases of the ASST task, and in PPC in the IDS phase of the task. The results demonstrate impairments of the areas associated with attentional functions in DAT+/− mice and show that an imbalance of the dopaminergic system has an impact on the complex attention-related executive functions. Highlights The effects of DAT depletion on cognitive processes related to attention were studied. Imbalance of the dopaminergic system impacts attention-related executive functions. Attentional deficits are accompanied by a lower activation of neurons in the IL, PrL and PPC regions.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  10. [해외논문]   Chronic ethanol exposure increases inhibition of optically targeted phasic dopamine release in the nucleus accumbens core and medial shell ex vivo   SCI SCIE

    Melchior, James R. (Corresponding author at: Department of Physiology and Pharmacology, Wake Forest School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157, United States.) , Jones, Sara R.
    Molecular and cellular neurosciences v.85 ,pp. 93 - 104 , 2017 , 1044-7431 ,

    초록

    Abstract Dopamine signaling encodes reward learning and motivated behavior through modulation of synaptic signaling in the nucleus accumbens, and aberrations in these processes are thought to underlie obsessive behaviors associated with alcohol abuse. The nucleus accumbens is divided into core and shell sub-regions with overlapping but also divergent contributions to behavior. Here we optogenetically targeted dopamine projections to the accumbens allowing us to isolate stimulation of dopamine terminals ex vivo. We applied 5 pulse (phasic) light stimulations to probe intrinsic differences in dopamine release parameters across regions. Also, we exposed animals to 4weeks of chronic intermittent ethanol vapor and measured phasic release. We found that initial release probability, uptake rate and autoreceptor inhibition were greater in the accumbens core compared to the shell, yet the shell showed greater phasic release ratios. Following chronic ethanol, uptake rates were increased in the core but not the shell, suggesting region-specific neuronal adaptations. Conversely, kappa opioid receptor function was upregulated in both regions to a similar extent, suggesting a local mechanism of kappa opioid receptor regulation that is generalized across the nucleus accumbens. These data suggest that dopamine axons in the nucleus accumbens core and shell display differences in intrinsic release parameters, and that ethanol-induced adaptations to dopamine neuron terminal fields may not be homogeneous. Also, chronic ethanol exposure induces an upregulation in kappa opioid receptor function, providing a mechanism for potential over-inhibition of accumbens dopamine signaling which may negatively impact downstream synaptic function and ultimately bias choice towards previously reinforced alcohol use behaviors. Highlights Optogenetic stimulation induces phasic dopamine release in NAc slices. Dopamine terminals in core and shell show intrinsic differences in release regulation. Chronic ethanol increases dopamine uptake rates in the NAc core. Chronic ethanol increases kappa opioid receptor inhibition of dopamine release.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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