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Microbial pathogenesis 48건

  1. [해외논문]   Editorial Board  


    Microbial pathogenesis v.112 ,pp. IFC , 2017 , 0882-4010 ,

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  2. [해외논문]   Editorial Board   SCI SCIE


    Microbial pathogenesis v.112 ,pp. IFC - IFC , 2017 , 0882-4010 ,

    초록

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   Beneficial immunomodulatory and neuro digestive effect in Trypanosoma cruzi infection after Lycopodium clavatum 13c treatment   SCI SCIE

    Brustolin Aleixo, Camila Fernanda (Corresponding author. Universidade Estadual de Maringá, Avenida Colombo, 5790, Jd. Universitário, bloco I90, sala 11, 87020-900, Maringá, Paraná, Brazil.) , Ferraz, Fabiana Nabarro , Massini, Paula Fernanda , Lopes, Carina Ribeiro , Falkowski Temporini, Gislaine Janaina , Aleixo, Denise Lessa , de Araú , jo, Silvana Marques
    Microbial pathogenesis v.112 ,pp. 1 - 4 , 2017 , 0882-4010 ,

    초록

    Abstract Studies show that highly diluted medications demonstrate benefits in treating infections, constituting an alternative for their treatment. The present study evaluated the effects of Lycopodium clavatum , dynamization 13c, in Wistar rats infected with T. cruzi . In this study 42 male rats were intraperitoneally inoculated with T. cruzi - Y strain and allocated into groups: IC (infected control group) and Ly (treated with L. clavatum 13c). The cytokines dosage (IFN-γ, IL-12, IL-10, IL-4), quantification and morphometry of myenteric neurons were evaluated. The treatment with L. clavatum modifies the immune response, with increase of IFN-γ on day 10 a.i. and IL-12 on day 24 a.i., decrease of IL-10 concentration on day 10 a.i. and subsequent increase of this cytokine and IL-4 on day 24 a.i., affording a bigger number of myenteric neurons compared to IC group. Thus, L. clavatum 13c promoted on rats infected with T. cruzi a beneficial immunomodulatory action reducing the pathogenic progression of digestive Chagas disease. Highlights L. clavatum 13c produced immunomodulatory action in rats infected with T. cruzi. Cytokine balance in treated animals was related with a bigger number of myenteric neurons. Treated animals presented neuron hypertrophy in distal and proximal colon. L. clavatum 13c reduced the pathogenic progression of digestive Chagas disease. Graphical abstract [DISPLAY OMISSION]

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  4. [해외논문]   Gut biofilm forming bacteria in inflammatory bowel disease   SCI SCIE

    Srivastava, Ankita (Faculty of Biotechnology, Institute of Biosciences and Technology, Shri Ramswaroop Memorial University, Lucknow-Deva Road, Vill: Hadauri, Post: Tindola, Dist: Barabanki 225003, Uttar Pradesh, India ) , Gupta, Jaya (Faculty of Biotechnology, Institute of Biosciences and Technology, Shri Ramswaroop Memorial University, Lucknow-Deva Road, Vill: Hadauri, Post: Tindola, Dist: Barabanki 225003, Uttar Pradesh, India ) , Kumar, Sunil (Faculty of Biotechnology, Institute of Biosciences and Technology, Shri Ramswaroop Memorial University, Lucknow-Deva Road, Vill: Hadauri, Post: Tindola, Dist: Barabanki 225003, Uttar Pradesh, India ) , Kumar, Awanish (Department of Biotechnology, National Institute of Technology, Raipur 492010, Chhattisgarh, India)
    Microbial pathogenesis v.112 ,pp. 5 - 14 , 2017 , 0882-4010 ,

    초록

    Abstract Inflammatory bowel disease (IBD) symbolizes a group of intestinal disorders in which prolonged inflammation occur in the digestive tract (esophagus, large intestine, small intestine mouth, stomach). Both genetic and environmental factors (infections, stress, diet) are involved in the development of IBD. As we know that bacteria are found in the intestinal mucosa of human and clinical observations revealed bacterial biofilms associated with patients of IBD. Various factors and microbes are found to play an essential role in biofilm formation and mucosal colonization during IBD. Biofilm formation in the digestive tract is dependent on an extracellular matrix synthesized by the bacteria and it has an adverse effect on the immune response of the host. There is no satisfactory and safe treatment option for IBD. Therefore, the current research aims to disrupt biofilm in IBD and concentrates predominantly on improving the drug. Here, we review the literature on bacterial biofilm and IBD to gather new knowledge on the current understanding of biofilm formation in IBD, host immune deregulation and dysbiosis in IBD, molecular mechanism, bacteria involved in biofilm formation, current and future regimen. It is urgently required to plan new ways to control and eradicate bacteria in biofilms that will open up novel diagnostic and therapeutic avenues for IBD. This article includes the mechanism of signaling molecules with respect to the biofilm-related genes as well as the diagnostic methods and new technologies involved in the treatment of IBD. Highlights Inflammatory bowel disease (IBD) is a group of intestinal disorders. Genetic and environmental factors are involved in the development of IBD. Various studies revealed that bacterial biofilms is associated with patients of IBD. There is no satisfactory and safe treatment option for IBD. The article includes mechanism of signaling molecules with respect to biofilm-related genes and diagnostic options of IBD.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Orlistat treatment induces apoptosis and arrests cell cycle in HSC-3 oral cancer cells   SCI SCIE

    Xiao, Xia (Department of Oral and Maxillofacial Surgery, Yan'an University Affiliated Hospital, Yanan, Shanxi, 16000, China ) , Liu, Huaiqin (Department of Stomatology, The First Hospital of Yulin, Yulin, Shanxi, 718000, China ) , Li, Xiaodong (Department of Stomatology, Yan'an People's Hospital, Yanan, Shanxi, 716000, China)
    Microbial pathogenesis v.112 ,pp. 15 - 19 , 2017 , 0882-4010 ,

    초록

    Abstract The present study was aimed to investigate the effect of orlistat on an oral squamous cancer line HSC-3 as well as the underlying mechanism. Cell Counting Kit-8 (CCK-8) (Dojindo, Shanghai, China) was used for the analysis of proliferation, Annexin V-FITC and propidium iodide staining for apoptosis and flow cytometry for cell cycle distribution. Western blot assay was used to determine the alteration in the expression of cyclin D1, B1, E and CDK1. The results revealed a concentration and time-dependent decrease in the proliferation of HSC-3 cells by orlistat. The viability of HSC-3 cells was reduced to 23.4 ± 2.5 and 15.7 ± 1.6% at 40 and 50 μM concentration of orlistat after 48 h. Treatment of HSC-3 cells with orlistat resulted induction of apoptosis significantly (p Highlights The present study was aimed to investigate the effect of orlistat on an oral squamous cancer line HSC-3 as well as the underlying mechanism. The results revealed a concentration and time-dependent decrease in the proliferation of HSC-3 cells by orlistat. The viability of HSC-3 cells was reduced to 23.4 ± 2.5 and 15.7 ± 1.6% at 40 and 50 μM concentration of orlistat after 48 h. Thus, orlistat induces apoptosis and cell cycle arrest in G2/M phase in HSC-3 cells through decrease in expression of cyclin D1 and E and increase in phosphorylation of CDK1. Therefore, orlistat can be used for the treatment of oral squamous cancer.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [해외논문]   Antigen 85 complex as a powerful Mycobacterium tuberculosis immunogene: Biology, immune-pathogenicity, applications in diagnosis, and vaccine design   SCI SCIE

    Karbalaei Zadeh Babaki, Mohsen (Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran ) , Soleimanpour, Saman (Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran ) , Rezaee, Seyed Abdolrahim (Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran)
    Microbial pathogenesis v.112 ,pp. 20 - 29 , 2017 , 0882-4010 ,

    초록

    Abstract Mycobacterium tuberculosis ( Mtb ) is one of the most life-threatening mycobacterial species which is increasing the death rate due to emerging multi-drug resistant (MDR) strains. Concerned health authorities worldwide are interested in developing an effective vaccine to prevent the spread of Mtb . After years of research, including successful identification of many Mtb immunogenic molecules, effective therapeutic agents or a vaccine have yet to be found. However, among the identified Mtb immunogenes, antigen 85 (Ag85) complex (Ag85A, Ag85B, and Ag85C) is receiving attention from scientists as it allows bacteria to evade the host immune response by preventing formation of phagolysosomes for eradication of infection. Due to their importance, A85 molecules are being utilized as tools in diagnostic methods and in the construction of new vaccines, such as recombinant attenuated vaccines, DNA vaccines, and subunit vaccines. This paper represents a comprehensive review of studies on Mtb molecules examining pathogenicity, biochemistry, immunology, and the role of Mtb in therapeutic or vaccine research.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [해외논문]   Identification and characterization of CD4+ T cell epitopes on manganese transport protein C of Staphylococcus aureus   SCI SCIE

    Yu, Wei (Corresponding author. College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, 163319, China.) , Wang, Lizi , Wang, Mengyao , Liu, Shuo , Li, Wanyu , Wang, Xintong , Li, Xiaoting , Yu, Simiao , Yao, Di , Ma, Jinzhu , Yu, Liquan , Chen, Jing , Feng, Zhenyue , Cui, Yudong
    Microbial pathogenesis v.112 ,pp. 30 - 37 , 2017 , 0882-4010 ,

    초록

    Abstract Manganese transport protein C (MntC) of Staphylococcus aureus represents an excellent vaccine-candidate antigen. The important role of CD4 + T cells in effective immunity against S. aureus infection was shown; however, CD4 + T cell-specific epitopes on S. aureus MntC have not been well identified. Here, we used bioinformatics prediction algorithms to evaluate and identify nine candidate epitopes within MntC. Our results showed that peptide M8 emulsified in Freund's adjuvant induced a much higher cell-proliferation rate as compared with controls. Additionally, CD4 + T cells stimulated with peptide M8 secreted significantly higher levels of interferon-γ and interleukin-17A. These results suggested that peptide M8 represented an H-2d (I-E)-restricted Th17-specific epitope. Highlights We investigated the potential of peptides derived from S. aureus MntC as potential CD4 + T cell-specific epitopes. We identified a novel immunodominant CD4 + T cell epitope of MntC by bioinformatics and experimentation. We revealed ability of the epitope to induce Th1 and Th17 differentiation and elicit specific cytokine secretion. We showed the efficacy of MntC and its epitope as vaccines against S. aureus infection.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [해외논문]   Inhibition of MEK-ERK1/2-MAP kinase signalling pathway reduces rabies virus induced pathologies in mouse model   SCI SCIE

    Manjunatha, Venkataravanappa (Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India ) , Singh, Karam Pal (Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India ) , Saminathan, Mani (Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India ) , Singh, Rajendra (Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India ) , Shivasharanappa, Nayakwadi (Animal Science Section, ICAR-Central Coastal Agricultural Research Institute, Ela, Goa, India ) , Umeshappa, Channakeshava Sokke (Cancer Research Unit, Saskatchewan Cancer Agency, University of Saskatchewan, Saskatoon, Canada ) , Dhama, Kuldeep (Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India ) , Manjunathareddy, Gundallahalli Bayyappa (ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India)
    Microbial pathogenesis v.112 ,pp. 38 - 49 , 2017 , 0882-4010 ,

    초록

    Abstract The extracellular signal-regulated kinase (ERK) pathway has been shown to regulate pathogenesis of many viral infections, but its role during rabies virus (RV) infection in vivo is not clear. In the present study, we investigated the potential role of MEK-ERK1/2 signalling pathway in the pathogenesis of rabies in mouse model and its regulatory effects on pro-inflammatory cytokines and other mediators of immunity, and kinetics of immune cells. Mice were infected with 25 LD 50 of challenge virus standard (CVS) strain of RV by intracerebral (i.c.) inoculation and were treated i.c. with U0126 (specific inhibitor of MEK1/2) at 10 μM/mouse at 0, 2, 4 and 6 days post-infection. Treatment with U0126 resulted in delayed disease development and clinical signs, increased survival time with lesser mortality than untreated mice. The better survival of inhibitor-treated and RV infected mice was positively correlated with reduced viral load and reduced viral spread in the brain as quantified by real-time PCR, direct fluorescent antibody test and immunohistochemistry. CVS-infected/mock-treated mice developed severe histopathological lesions with increased Fluoro-Jade B positive degenerating neurons in brain, which were associated with higher levels of serum nitric oxide, iNOS, TNF-α, and CXCL10 mRNA. Also CVS-infected/U0126-treated mice revealed significant decrease in caspase 3 but increase in Bcl-2 mRNA levels and less TUNEL positive apoptotic cells. CVS-infected/U0126-treated group also showed significant increase in CD4 + , CD8 + T lymphocytes and NK cells in blood and spleen possibly due to less apoptosis of these cells. In conclusion, these data suggest that MEK-ERK1/2 signalling pathway play critical role in the pathogenesis of RV infection in vivo and opens up new avenues of therapeutics. Highlights MEK-ERK-1/2 inhibition delayed development of clinical signs and increased survival time in rabies virus infection. CVS-infected/U0126-treated mice developed severe histopathological lesions, higher cytokines levels and viral load. MEK1/2 inhibition reduced apoptosis and increased CD4 + , CD8 + T lymphocytes and NK cells.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  9. [해외논문]   Emergence of class 1 to 3 integrons among members of Enterobacteriaceae in Egypt   SCI SCIE

    Rizk, Dina E. (Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt ) , El-Mahdy, Areej M. (Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt)
    Microbial pathogenesis v.112 ,pp. 50 - 56 , 2017 , 0882-4010 ,

    초록

    Abstract In spite of the role of integrons as the main contributor to multidrug resistance worldwide, their prevalence in Egypt is still underestimated. In this work, we announce the emergence of class 2 and 3 integrons among Enterobacteriacae isolates from Mansoura University Hospitals. Ninety-three clinical isolates were obtained from different clinical sources, among which 70% of E. coli , 94.8% of K. pneumonia e and 85.7% of Enterobacter spp. were assigned to be multidrug resistant (MDR). Subsequently, the occurrence of class 1–3 integrons was confirmed by multiplex PCR. Class 1 integron was the most predominant being harbored by 42.8%, 90% and 25% of MDR E. coli , K. pneumonia e and Enterobacter spp. isolates, respectively. This was followed by class 2 and 3 integrons which were, for the first time, reported in these hospitals. Also, coexistence of integrons 1and 2 was revealed in 36.9% of integron positive isolates. A significant association was noticed only between resistance to gentamicin and integron prevalence among MDR E. coli isolates (P = 0.02). In conclusion, this work represents the first report for detection of class 2 and 3 integrons, beside the previously detected class 1 integrons. This highlights the high incidence of integrons among MDR Enterobacteriacae isolates which indicates the selective pressure of antibiotics in these hospitals. Moreover, this study confirms the possibility of the use of integrons as markers for MDR identification.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  10. [해외논문]   Melittin and its potential in the destruction and inhibition of the biofilm formation by Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa isolated from bovine milk   SCI SCIE

    Picoli, Tony (Laboratory of Virology and Immunology, Federal University of Federal de Pelotas, Pelotas, RS, Brazil ) , Peter, Cristina Mendes (Laboratory of Virology and Immunology, Federal University of Federal de Pelotas, Pelotas, RS, Brazil ) , Zani, Joã (Laboratory of Bacteriology and Public Health, Federal University of Pelotas, Pelotas, RS, Brazil ) , o Luí (Center of Diagnosis and Research in Veterinary Mycology, Federal University of Pelotas, Pelotas, RS, Brazil ) , z (Laboratory of Virology and Immunology, Federal University of Federal de Pelotas, Pelotas, RS, Brazil ) , Waller, Stefanie Bressan (Laboratory of Virology and Immunology, Federal University of Federal de Pelotas, Pelotas, RS, Brazil ) , Lopes, Matheus Gomes (Laboratory of Virology and Immunology, Federal University of Federal de Pelotas, Pelotas, RS, Brazil ) , Boesche, Kamilla Neutzling (Laboratory of Virology and Immunology, Federal University of Federal de Pelotas, Pelotas, RS, Brazil ) , Vargas, Gilberto D´Á (Laboratory of Virology and Immunology, Federal University of Federal de Pelotas, Pelotas, RS, Brazil) , vila , Hü , bner, Silvia de Oliveira , Fischer, Geferson
    Microbial pathogenesis v.112 ,pp. 57 - 62 , 2017 , 0882-4010 ,

    초록

    Abstract Staphylococcus aureus , Escherichia coli and Pseudomonas aeruginosa stand out in veterinary and human medicine for their role in opportunistic infections and their pathogenic mechanisms, including the biofilms formation. It was investigated the antibacterial activity of melittin and antibiofilm of such bacteria. Twelve strains of these microorganisms isolated from bovine milk were used, as well as the strains S. aureus ATCC 12600, E. coli ATCC 8739 and Pseudomonas aeruginosa ATCC 15442. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution technique. The biofilms were formed in 96-well plates and melittin on these colonies was added at different concentrations and times. Bacteria previously exposed to melittin were evaluated for inhibition of biofilm production. The MIC and MBC were respectively in μg/mL: S. aureus (6–7 and 32–64), E. coli (40–42.5 and 64–128) and P. aeruginosa (65–70 and 64–128). S. aureus biofilms were more sensitive to the action of melittin, since upon exposure to a concentration 10 times lower than the MIC for 4 h, was completely destroyed. In Gram negative bacteria, the pre-formed biofilm was destroyed only when exposed for 4 h under the MIC. With respect to inhibition of biofilm production, S. aureus was the most sensitive again because produced only 37.2% of the biofilm formed by the control (without previous exposure to melittin), when exposed to the MIC, and at a concentration hundred times smaller than MIC, this microorganism produced 75.2% of the biofilm. E. coli was the most resistant bacteria and produced 56.3% of the biofilm, even if previously exposed to melittin MIC. Melittin presents desirable effects in combating microorganisms studied both at your disposal, biofilm destruction and inhibition of the formation, and maybe used in future studies of new strategies to combat infections caused by these pathogens. Highlights Melittin has antibacterial action and highlights the greater sensitivity of Gram-positive than Gram-negative bacterias. Melittin has the ability to destroy biofilms formed by pathogenic bacteria present in bovine milk. Pathogenic bacteria present in bovine milk decrease biofilm production when previously exposed to melittin.

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