본문 바로가기
HOME> 저널/프로시딩 > 저널/프로시딩 검색상세

저널/프로시딩 상세정보

권호별목차 / 소장처보기

H : 소장처정보

T : 목차정보

Journal of the Korean society for horticultural sc...Journal of the Korean society for horticultural science 10건

  1. [국내논문]   Agrobacterium-Mediated Transformation of Lettuce with a Terpene Synthase Gene  

    Dong Wook Cho ; Young Doo Park ; Kyu Hwan Chung
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 169 - 175 , 2005 , 0253-6498 ,

    초록

    The amorpha-4, 11-diene synthase gene isolated from Artemisia annua was inserted into the binary vector pILTAB 357 within restriction enzyme sites, EcoRI and BamHI. It was introduced into the lettuce through Agrobacterium-mediated transformation. A total of nine transgenic plants were obtained from the inoculated lettuce leaf disc. The integration of the amorpha-4, diene synthase gene into the lettuce genome was confirmed by polymerase chain reaction (PCR) and Southern hybridization. The PCR products of 1.6 kb have been produced from all transgenic plants. From the Southern blot analysis, the amorpha-4, 11-diene synthase gene was transferred to nine transgenic lettuce plants with one to three copies. The antibiotics resistance of T1 transgenic plants was tested by growing on medium supplemented with kanamycin sulfate (0, 50, and 100 ㎎ㆍL⁻¹). In the result, the segregation ratio of T1 transgenic plants was exhibited about 2.7:1, 638 individuals of kanamycin-resistant and 236 of kanamycin-sensitive. The inheritance characteristics of amorpha-4, 11-diene synthase gene in the T1 transgenic plants was analyzed by PCR. The 1.6 kb PCR products were produced from nine lines of T1 transgenic plants and its segregation ratio was 1:1. The artemisinin content of T1 transgenic lettuces was analyzed through gas chromatography and mass spectrometry. The gas chromatography analysis showed that the multi-peaks were produced from nine lines of T1 transgenic plants at a retention time of 10 min 7 s. Thus, mass spectrometric analysis of the T1 transgenic plants was performed. In the result, however, no T1 transgenic plants produce any peak corresponding to artemisinin.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  2. [국내논문]   Storage Potential of Early-Season Cultivar `Tsugaru' Apples Based on Consumer Acceptance after Marketing Simulation  

    Park, Y.-M. ; Yoon, T. M.
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 176 - 182 , 2005 , 0253-6498 ,

    초록

    The amorpha-4, 11-diene synthase gene isolated from Artemisia annua was inserted into the binary vector pILTAB 357 within restriction enzyme sites, EcoRI and BamHI. It was introduced into the lettuce through Agrobacterium-mediated transformation. A total of nine transgenic plants were obtained from the inoculated lettuce leaf disc. The integration of the amorpha-4, diene synthase gene into the lettuce genome was confirmed by polymerase chain reaction (PCR) and Southern hybridization. The PCR products of 1.6 kb have been produced from all transgenic plants. From the Southern blot analysis, the amorpha-4, 11-diene synthase gene was transferred to nine transgenic lettuce plants with one to three copies. The antibiotics resistance of T1 transgenic plants was tested by growing on medium supplemented with kanamycin sulfate (0, 50, and 100 ㎎ㆍL⁻¹). In the result, the segregation ratio of T1 transgenic plants was exhibited about 2.7:1, 638 individuals of kanamycin-resistant and 236 of kanamycin-sensitive. The inheritance characteristics of amorpha-4, 11-diene synthase gene in the T1 transgenic plants was analyzed by PCR. The 1.6 kb PCR products were produced from nine lines of T1 transgenic plants and its segregation ratio was 1:1. The artemisinin content of T1 transgenic lettuces was analyzed through gas chromatography and mass spectrometry. The gas chromatography analysis showed that the multi-peaks were produced from nine lines of T1 transgenic plants at a retention time of 10 min 7 s. Thus, mass spectrometric analysis of the T1 transgenic plants was performed. In the result, however, no T1 transgenic plants produce any peak corresponding to artemisinin.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  3. [국내논문]   Micropropagation from Stem Segments of Salt Tolerant Jojoba Seedlings  

    Meyghani, H. ; Ghazvini, R. F. ; Hamidoghli, Y.
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 183 - 187 , 2005 , 0253-6498 ,

    초록

    The amorpha-4, 11-diene synthase gene isolated from Artemisia annua was inserted into the binary vector pILTAB 357 within restriction enzyme sites, EcoRI and BamHI. It was introduced into the lettuce through Agrobacterium-mediated transformation. A total of nine transgenic plants were obtained from the inoculated lettuce leaf disc. The integration of the amorpha-4, diene synthase gene into the lettuce genome was confirmed by polymerase chain reaction (PCR) and Southern hybridization. The PCR products of 1.6 kb have been produced from all transgenic plants. From the Southern blot analysis, the amorpha-4, 11-diene synthase gene was transferred to nine transgenic lettuce plants with one to three copies. The antibiotics resistance of T1 transgenic plants was tested by growing on medium supplemented with kanamycin sulfate (0, 50, and 100 ㎎ㆍL⁻¹). In the result, the segregation ratio of T1 transgenic plants was exhibited about 2.7:1, 638 individuals of kanamycin-resistant and 236 of kanamycin-sensitive. The inheritance characteristics of amorpha-4, 11-diene synthase gene in the T1 transgenic plants was analyzed by PCR. The 1.6 kb PCR products were produced from nine lines of T1 transgenic plants and its segregation ratio was 1:1. The artemisinin content of T1 transgenic lettuces was analyzed through gas chromatography and mass spectrometry. The gas chromatography analysis showed that the multi-peaks were produced from nine lines of T1 transgenic plants at a retention time of 10 min 7 s. Thus, mass spectrometric analysis of the T1 transgenic plants was performed. In the result, however, no T1 transgenic plants produce any peak corresponding to artemisinin.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  4. [국내논문]   Interrelations among Crop Load, Fruit Quality, Shoot Growth, and Leaf Mineral Nutrients in Dense-Planting `Fuji'/M.26/Seedling Apple Trees with High Productivity  

    Yim, Y. J.
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 188 - 192 , 2005 , 0253-6498 ,

    초록

    The amorpha-4, 11-diene synthase gene isolated from Artemisia annua was inserted into the binary vector pILTAB 357 within restriction enzyme sites, EcoRI and BamHI. It was introduced into the lettuce through Agrobacterium-mediated transformation. A total of nine transgenic plants were obtained from the inoculated lettuce leaf disc. The integration of the amorpha-4, diene synthase gene into the lettuce genome was confirmed by polymerase chain reaction (PCR) and Southern hybridization. The PCR products of 1.6 kb have been produced from all transgenic plants. From the Southern blot analysis, the amorpha-4, 11-diene synthase gene was transferred to nine transgenic lettuce plants with one to three copies. The antibiotics resistance of T1 transgenic plants was tested by growing on medium supplemented with kanamycin sulfate (0, 50, and 100 ㎎ㆍL⁻¹). In the result, the segregation ratio of T1 transgenic plants was exhibited about 2.7:1, 638 individuals of kanamycin-resistant and 236 of kanamycin-sensitive. The inheritance characteristics of amorpha-4, 11-diene synthase gene in the T1 transgenic plants was analyzed by PCR. The 1.6 kb PCR products were produced from nine lines of T1 transgenic plants and its segregation ratio was 1:1. The artemisinin content of T1 transgenic lettuces was analyzed through gas chromatography and mass spectrometry. The gas chromatography analysis showed that the multi-peaks were produced from nine lines of T1 transgenic plants at a retention time of 10 min 7 s. Thus, mass spectrometric analysis of the T1 transgenic plants was performed. In the result, however, no T1 transgenic plants produce any peak corresponding to artemisinin.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  5. [국내논문]   Local Strain Selection of Dendranthema zawadskii Complex Native to Korea for Rhizome Mass Production  

    Lee, C. H. ; Kim, K. S.
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 193 - 199 , 2005 , 0253-6498 ,

    초록

    The amorpha-4, 11-diene synthase gene isolated from Artemisia annua was inserted into the binary vector pILTAB 357 within restriction enzyme sites, EcoRI and BamHI. It was introduced into the lettuce through Agrobacterium-mediated transformation. A total of nine transgenic plants were obtained from the inoculated lettuce leaf disc. The integration of the amorpha-4, diene synthase gene into the lettuce genome was confirmed by polymerase chain reaction (PCR) and Southern hybridization. The PCR products of 1.6 kb have been produced from all transgenic plants. From the Southern blot analysis, the amorpha-4, 11-diene synthase gene was transferred to nine transgenic lettuce plants with one to three copies. The antibiotics resistance of T1 transgenic plants was tested by growing on medium supplemented with kanamycin sulfate (0, 50, and 100 ㎎ㆍL⁻¹). In the result, the segregation ratio of T1 transgenic plants was exhibited about 2.7:1, 638 individuals of kanamycin-resistant and 236 of kanamycin-sensitive. The inheritance characteristics of amorpha-4, 11-diene synthase gene in the T1 transgenic plants was analyzed by PCR. The 1.6 kb PCR products were produced from nine lines of T1 transgenic plants and its segregation ratio was 1:1. The artemisinin content of T1 transgenic lettuces was analyzed through gas chromatography and mass spectrometry. The gas chromatography analysis showed that the multi-peaks were produced from nine lines of T1 transgenic plants at a retention time of 10 min 7 s. Thus, mass spectrometric analysis of the T1 transgenic plants was performed. In the result, however, no T1 transgenic plants produce any peak corresponding to artemisinin.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  6. [국내논문]   Effects of Temperature and Daylength on Rooting and Growth of Elatior Begonia Cuttings  

    Myung-Syun Shim ; Wook Oh ; Ki Sun Kim
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 200 - 203 , 2005 , 0253-6498 ,

    초록

    The research was carried out to investigate the effects of daylength and temperature on rooting and growth of Elatior begonia cuttings. Begonia × hiemalis "Britt Dark" and "Line" were selected as plant materials and the experiments Were conducted in a multi-room incubator. The cuttings were subjected to 16 h or 13/16 h daylength in combination with 18 or 20℃ until 2 weeks after cutting and then transferred to 16 h. The number of leaves, root and stem length, and fresh and dry weight of cuttings were increased at 20℃ than at 18℃. Moreover, the number of days to rooting of the cuttings were reduced. The temperature treatment was more effective for the growth of cuttings than the day length treatment. Temperature treatments had a statistically significant effect on the development and growth of roots, whereas there was no significant difference among the daylength treatments. However, continuous daylength of 16 h was more effective in improving the growth of cuttings. The cuttings were also treated separately at 18, 20, 22, or 24℃. As the temperature increased, the growth of cuttings tended to become more superior. The cuttings treated at 24℃ showed the best shoot and root growth. In conclusion, 16 h daylength and 24℃ temperature were the most effective in promoting rooting and plant growth of Elatior begonia cuttings.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  7. [국내논문]   Enhancement of Drought Tolerance with Uniconazole Application in Epiprenum aureum during Hydroculture or Soil Culture  

    Baik, J. ; Nam, Y. K. ; Pak, C. H.
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 204 - 209 , 2005 , 0253-6498 ,

    초록

    The research was carried out to investigate the effects of daylength and temperature on rooting and growth of Elatior begonia cuttings. Begonia × hiemalis "Britt Dark" and "Line" were selected as plant materials and the experiments Were conducted in a multi-room incubator. The cuttings were subjected to 16 h or 13/16 h daylength in combination with 18 or 20℃ until 2 weeks after cutting and then transferred to 16 h. The number of leaves, root and stem length, and fresh and dry weight of cuttings were increased at 20℃ than at 18℃. Moreover, the number of days to rooting of the cuttings were reduced. The temperature treatment was more effective for the growth of cuttings than the day length treatment. Temperature treatments had a statistically significant effect on the development and growth of roots, whereas there was no significant difference among the daylength treatments. However, continuous daylength of 16 h was more effective in improving the growth of cuttings. The cuttings were also treated separately at 18, 20, 22, or 24℃. As the temperature increased, the growth of cuttings tended to become more superior. The cuttings treated at 24℃ showed the best shoot and root growth. In conclusion, 16 h daylength and 24℃ temperature were the most effective in promoting rooting and plant growth of Elatior begonia cuttings.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  8. [국내논문]   Direct Somatic Embryogenesis and Plant Regeneration in Cineraria (Senecio cruentus)  

    Eun Young Nam ; Gyeong Hee Kim ; Byoung Ryong Jeong
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 210 - 216 , 2005 , 0253-6498 ,

    초록

    This study was conducted to investigate the conditions involved in efficient somatic embryogenesis and plant regeneration of cineraria "Jester Pink". Explants were taken from cotyledons and hypocotyls. Initiation and development of somatic embryos occurred through a two-step process. Explants were first induced to globular embryos on the MS medium supplemented with several plant growth regulators (4.5-13.5 μM 2,4-dichlorophenoxy acetic acid and 1.5-4.5 μM benzylademine, kinetin, or thidiazuron). Somatic embryos formed directly on the surface of explants without the callus phase after 5 weeks of culture. In the next step, somatic embryos were transferred to the plant growth regulator-free or the medium supplemented with 3.8-11.4 μM abscisic acid, 0.1-0.3% (w/v) polyethylene glycol and 4-6% (w/v) sucrose for embryo maturation. Somatic embryos regenerated to plantlets through globular, heart, torpedo, and cotyledonary stages as do the zygotic embryos. Abscisic acid was more efficient than polyethylene glycol or high concentration sucrose for embryo maturation. The regenerated plantlets grown in a greenhouse showed morphological identity to the donor plant and flowered normally.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  9. [국내논문]   Rooting Characteristics of Stem Tip Cuttings in Ardisia pusilla as Influenced by Cutting Stage, Rooting Medium, Temperature, and Plant Growth Regulator Pretreatment  

    Chang Hee Lee ; Oh Keun Kwon ; Young Jin Kim
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 217 - 224 , 2005 , 0253-6498 ,

    초록

    To produce commercially acceptable pot plants of Ardisia pusilla, vegetative propagation method such as stem tip cutting has been used in general. Thus, the production of uniform and vigorous rooted cuttings is required. This study was conducted to screen an adequate stem tip cutting stage for rooting, to select an adequate rooting medium, to find its favoring temperature for rooting, and to develop a suitable method for promoting root development. The stages of stem tip cuttings could be divided into five groups by the degree of leaf expansion and chlorophyll contents. Stem tip cutting with green leaves was found to be the best rooting potential among the five types of stem tip cuttings. In rooting medium, roots dry weight showed 152% increase in only vermiculite compared to in a commercial growing medium (perlite:peatmoss=1:1, v/v). Rooted cuttings of A. pusilla showed very sensitive rooting potential according to temperature and optimal rooting temperature was found to be 20-25℃. Although indole-3-butyric acid (IBA) used for rooting increased the number of roots in a concentration-dependent manner regardless of the pretreating methods, dipping treatment into a 1500 ㎎ㆍℓ⁻¹ IBA solution for 10 min was the most effective on increasing the number of roots. However, cuttings treated with 0.4% naphthylacetamide (NAA) talc showed the heaviest root dry weight than those with the other treatments. Thus, NAA talc was the most acceptable rooting promotion method for producing rooted cuttings due to simple and practical method used by farmers. In conclusion, rooting characteristics of cuttings in A. pusilla was very dependent on cutting stage, rooting medium, and rooting temperature.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  10. [국내논문]   Genetic Diversity and Population Structure of Acanthopanax sessiliflorus Using Random Amplified Polymorphic DNA Markers  

    Huh, M. K. ; Huh, H. W. ; Choi, J. S.
    Journal of the Korean society for horticultural science v.46 no.3 ,pp. 225 - 230 , 2005 , 0253-6498 ,

    초록

    To produce commercially acceptable pot plants of Ardisia pusilla, vegetative propagation method such as stem tip cutting has been used in general. Thus, the production of uniform and vigorous rooted cuttings is required. This study was conducted to screen an adequate stem tip cutting stage for rooting, to select an adequate rooting medium, to find its favoring temperature for rooting, and to develop a suitable method for promoting root development. The stages of stem tip cuttings could be divided into five groups by the degree of leaf expansion and chlorophyll contents. Stem tip cutting with green leaves was found to be the best rooting potential among the five types of stem tip cuttings. In rooting medium, roots dry weight showed 152% increase in only vermiculite compared to in a commercial growing medium (perlite:peatmoss=1:1, v/v). Rooted cuttings of A. pusilla showed very sensitive rooting potential according to temperature and optimal rooting temperature was found to be 20-25℃. Although indole-3-butyric acid (IBA) used for rooting increased the number of roots in a concentration-dependent manner regardless of the pretreating methods, dipping treatment into a 1500 ㎎ㆍℓ⁻¹ IBA solution for 10 min was the most effective on increasing the number of roots. However, cuttings treated with 0.4% naphthylacetamide (NAA) talc showed the heaviest root dry weight than those with the other treatments. Thus, NAA talc was the most acceptable rooting promotion method for producing rooted cuttings due to simple and practical method used by farmers. In conclusion, rooting characteristics of cuttings in A. pusilla was very dependent on cutting stage, rooting medium, and rooting temperature.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지

논문관련 이미지