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권호별목차 / 소장처보기

H : 소장처정보

T : 목차정보

한국수산학회지 = Journal of the Korean Fisheries Society 24건

  1. [국내논문]   Synopsis of the Family Dasyatidae (Elasmobranchii, Rajiformes) from Korea  

    LEE Chung Lyul (Department of Biology, Kunsan National University ) , JOO Dong Soo (Department of Biology, Kunsan National University)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 745 - 753 , 1996 , 0374-8111 ,

    초록

    Taxonomic revision of the family Dasyatidae was studied on the basis of the specimens collected from the Korean coasts from June 1994 to January 1996. The family Dasyatidae of Korea was classified into 6 species in genus Dasyatis, and the key to species was proposed with their synonyms and distributions. Three new records from Korea were described and figured in detail: Dasyatis acutirostra Nishida and Nakaya, D. matsubarai Miyosi and D. sinensis (Steindachner). Most species of the Korean stingrays are shared with those of China and Japan.

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  2. [국내논문]   Effect of Washing and Additives on Gel Formation of Squid Surimi  

    LEE Nahm-Gull (Department of Food Science and Technology, Pukyong National University ) , CHO Young-Je (Department of Food Science and Technology, Pukyong National University)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 754 - 760 , 1996 , 0374-8111 ,

    초록

    Effects of washing and additives on the texture of squid surimi gel which has been known to hard to gelation due to high protease activities and many water solubles were studied by SDS-PAGE, compression test, jelly strength and transmission electron microscopy analysis (TEM). Myosin (205 kDa) heavy chain was the major protein in water soluble fractions. It was impossible to make a gel after washing of the minced squid meat. These results suggested that squid (Todarodes pacificus) minced meat does not need a washing for good jelly products. $3.0\%$ of bovine plasma protein (BPP) produced the hardest gel ( $16\%$ harder than the control) among the additives including egg white (EW), potato extracts (PE) and transglutaminase-K (TG-K) by compression test (P>0.05). Microstructure of control, $2\%$ EW and $4\%$ TG-K treated gels showed a sponge-like structure with more vacant space. Gels containing $3\%$ BPP formed the most rigid and arranged networks. Those results indicates that poor gel-network formation Was due to the degradation of myofibrillar proteins by proteases contained in the minced meat, which result in non-interlinkage.

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  3. [국내논문]   Random Amplified Polymorphic DNA (RAPD) Identification of Genetic Variation in Chlorella species  

    CHO Jung Jong (Department of Microbiology, Pukyong National University ) , KIM Yong-Tae (Department of Chemistry, Aoyama Gakuin University ) , HUR Sung Bum (Department of Aquaculture, Pukyong National University ) , KIM Young Tae (Department of Microbiology, Pukyong National University)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 761 - 769 , 1996 , 0374-8111 ,

    초록

    The random amplified polymorphic DNA (RAPD) technique was used to characterize 18 reference strains of microalgae, mostly Chlorella species, collected from various localities around Korea peninsular. Eighteen strains consist of four genera of the family marine Chlorella from 12 samples, two genera of fresh water Chlorella from three samples, and three genera on Nannochloris. Twenty 10-mer anonymous primers were screened for amplification of genomic DNA extracted from samples using the CTAB extraction method. Nineteen of these oligonucleotide primers were positive or band producing. Three of 20 random primers (OPA 10, OPA 12, and OPA 18) resulted in both clear band and a high degree of reproducibility and showed some potential to be used to discriminate individual samples of both genetically hetero-and homogeneous populations, in determining phylogenetic relationships between species within a genus and developing individual fingerprints for each samples.

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  4. [국내논문]   Molecular Characterization of Seaweeds Using RAPD and Differential Display  

    HONG Yong-Ki (Department of Biotechnology, Pukyong National University ) , KIM Yong-Tae (Department of Chemisttry, Aoyama Gakuin University ) , KIM Se-Kwon (Department of Chemistry, Pukyong National University)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 770 - 778 , 1996 , 0374-8111 ,

    초록

    A rapid and economical method of simultaneous extraction of DNA and RNA from seaweeds has been developed by the use of lithium chloride. Lithium chloride facilitates the softening of cell walls resulting in a decrease in both compressive and tensile modulus of elasticity. The DNA was characterized by high molecular weight larger than 27 kb and a relative lack of carbohydrate and protein contamination. The DNA and RNA extracted by the method from many seaweeds were of sufficient quality to be used as a template for per amplification with a plant intergenic gene primer set, for RAPD analysis with arbitrary primers, and for differential display with arbitrary primers in the morphologically distinct regions of the matured Porphyra thallus. The cDNA polymorphism indicated that the reproductive tissue types (male, female, patch) had a relatively high degree of similarity; the vegetative tissue types (dividing, non-dividing) also showed a similar pattern with respect to each other. Holdfast tissue had very low similarity with the other tissues, but appeared most similar to vegetative non-dividing tissue type.

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  5. [국내논문]   The Importance of Nitrogen Release and Denitrification in Sediment to the Nitrogen Budget in Hiroshima Bay  

    KIM Do-Hee (Department of Environmental Engineering, Pukyong National University ) , MATSUDA Osamu (Department of Aquatic Environmental Biology, Faculty of Applied Biological Science, Hiroshima University)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 779 - 786 , 1996 , 0374-8111 ,

    초록

    The main purpose of this study was to estimate the role of dissolved inorganic nitrogen (DIN) released from sediment and denitrification process in sediment on the nitrogen budget of Hiroshima Bay by means of collecting data on distributions and budgets of nitrogen and phosphorus in the bay, DIN fluxes across sediment-water interface and denitrification rates in the sediments of the same area. The TN : TP and DIN:DIP atomic ratios of the discharged freshwater were about 26 and 21, respectively. The standing stocks in the seawater of the TN : TP atomic ratio varied from 8 to 14 with an annual mean value of 11, while the DIN : DIP atomic ratio varied from 10 to 15 with an annual mean value of 12 in the bay. The residence time of nitrogen and phosphorus were estimated to be about 109 days and 200 days in the bay, respectively. The proportion of DIN released from sediment and denitrification rate to the loading of total nitrogen into Hiroshima Bay were $45\%\;(37\~82\%)\;and\;13\%(0.0\~37\%)$ , respectively, and the amount of nitrogen through denitrification process was 6.5 times larger than the outflow of nitrogen from the bay. The results show that DIN released from sediment and denitrification process in sediment play important roles on the nitrogen budget in Hiroshima Bay.

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  6. [국내논문]   The Biodegradation Characteristics of the Mixtures of Bunker-A, B Oils with Dispersants in the Seawater  

    BAEK Joong-Soo (Department of Environmental Engineering, Pukyong National University ) , KIM Gwang-Su (Faculty of Maritime Transportation System, Mokpo National Maritime University ) , CHO Eun-il (Department of Environmental Engineering, Pukyong National University)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 787 - 796 , 1996 , 0374-8111 ,

    초록

    The biodegradation experiment, the TOD analysis and the element analysis for dispersant, Bunker-A oil and Bunker-B oil were conducted to study the biodegradation characteristics of a mixture of Bunker-A oil with dispersant and a mixture of Bunker-B oil with dispersant in the seawater. The results of biodegradation experiment showed 1mg of dispersant to be equivalent to 0.26 mg of $BOD_5$ and to 0.60 mg of $BOD_{20}$ in the natural seawater. The results of TOD analysis showed each 1 mg of dispersant, Bunker-A oil and Bunker-B oil to be equivalent to 2.37 mg, 2.94 mg and 2.74 mg of TOD, respectively. The results of element analysis showed carbon, hydrogen, nitrogen and phosphorus contents of dispersant to be $82.1\%,\;13.8\%,\;1.8\%\;and\;2.2\%$ , respectively. Carbon and hydrogen contents of Bunker-A oil were found to be $73.3\%\;and\;13.5\%$ , respectively, and carbon, hydrogen and nitrogen contents of Bunker-B oil to be $80.4\%,\;12.3\%\;and\;0.7\%$ , respectively. Accordingly, the detection of nitrogen and phosphorus in dispersant shows that dispersants should be used with caution in coastal waters, with relation to eutrophication. The biodegradability of dispersant expressed as the ratio of $BOD_5/TOD$ was found to be $11.0\%$ . As the mix ratios of dispersant to Bunker-A oil (3 mg/l) and a mixture of Bunker-B oil (3mg/l) were changed from 1 : 10 to 5 : 10, the biodegradabilities of a mixture of Bunker-A oil with dispersant and Bunker-B oil with dispersant increased from $2.1\%\;to\;7.2\%$ and from $1.0\%\;to\;4.4\%$ , respectively. Accordingly, the dispersant belongs to the organic matter group of middle-biodegradability while mixtures in the mix ratio range of $1:10\~5:10$ belong to the organic matter group of low-biodegradability. The deoxygenation rate constant $(K_1)$ and ultimate biochemical oxygen demand $(L_0)$ obtained from the biodegradation experiment and Thomas slope method were found to be 0.125/day and 2.487 mg/l for dispersant (4 mg/l), respectively. $K_1\;and\;L_0$ , were found to be $0.079\~0.131/day$ and $0.318\~2.052\;mg/l$ for a mixture of Bunker-A oil with dispersant and to be $0.106\~0.371/day$ and $0.262\~1.106\;mg/l$ for a mixture of Bunker-B oil with dispersant, respectively, having $1:10\~5:10$ mix ratios of dispersant to Bunker-A oil and Bunker-B oil. The ultimate biochemical oxygen demands of the mixtures increased as the mix ratio of dispersant to Bunker-A, B oils changed from 1 : 10 to 5 : 10. This suggests that the more dispersants are applied to the sea for the cleanup of Bunker-A oil or Bunker-B oil, the more decreases the dissolved oxygen level in the seawater.

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  7. [국내논문]   Purification and Characterization of Trypsins Affecting on the Autolysis of Shrimp, Penaeus japonicus  

    KIM Hyeung-Rak (Department of Food Science and Nutrition, Yosu National Fisheries University ) , KIM Doo-Sang (Department of Food and Life Science, Pukyong National University ) , AHN Chang-Bum (Department of Food Science and Nutrition, Yosu National Fisheries University ) , PYEUN Jae-Hyeung (Department of Food and Life Science, Pukyong National University)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 797 - 804 , 1996 , 0374-8111 ,

    초록

    Two trypsins were purified from shrimp hepatopancreas through ammonium sulfate fractionation, Q-Sepharose ionic exchange, benzamidine Sepharose-6B affinity, and Sephacryl S-300 gel chromatography. Both enzymes had a single polypeptide chain with a molecular weight (M.W.) of 32 kDa by sodium dodecylsulfate polyacrylamide gel electrophoresis (SOS-PAGE), although trypsin A and B were estimated to be a molecular weight of 27.2 and 22.8 kDa, respectively, using Sephacryl S-300 gel filtration. Both trypsins had similar amino acid compositions and rich in glycine, valine, alanine, aspartic acid, and glutamic acid, but low in methionine and basic amino acids. Both enzymes were completely inactivated by soybean trypsin inhibitor (SBTI), phenylmethylsulfonyl fluoride (PMSF), tosyl-L-lysine chloromethyl ketone (TLCK), benzamidine, leupeptin, however, not affected by tosyl-L-phenylalanine chloromethyl ketone (TPCK) and pepstatin.

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  8. [국내논문]   Mysidacea (Crustacea) from the West Coast of Korea  

    Jo Soo-Gun (Department of Marine Living Resources, Kunsan National University ) , MA Chae-Woo (Department of Resources Science, College of Natural Sciences, Soonchunhyang University)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 805 - 827 , 1996 , 0374-8111 ,

    초록

    Fifteen mysid species representing 4 genera are reported from the west coast of Korea, Of these, Archaeomysis vulgaris (Nakazawa), Archaeomysis japonica Hanamura, Jo and Murano and Acanthomysis tenuicauda Murano are new to the Korean fauna, Acanthomysis aspera li, Acanthomysis fujinagai li, Acanthomysis japonica Nakazawa and Acanthomysis okayamaensis li are new to the western Korean fauna. Detailed description with illustrative figures, ecological notes and keys to species are given.

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  9. [국내논문]   Effects of Ammonia Concentration on Histological and Physiological Status in Black Seabream (Acanthopagrus schlegeli)  

    KWON Joon Yeong (Department of Aquaculture, Pukyong National University ) , CHANG Young Jin (Department of Aquaculture, Pukyong National University)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 828 - 836 , 1996 , 0374-8111 ,

    초록

    The histological changes of gill, liver, spleen and muscle, and respiration and blood variables and liver glycogen content were examined in black seabream, Acanthopagrus schlegeli. Fish were exposed to a high level of total ammonia nitrogen (10.4 mg/l) and recovered from exposure $(0.4{\pm}0.2mg/l)$ in a closed recirculating seawater system. In the process of exposure, mortality was $9\%$ , and hyperplasia, necrosis or inflammation appeared in all tissues except for muscle. Oxygen consumption was decreased by $49\%$ , and red blood cell (RBC) number, hematocrit and hemoglobin concentration were significantly decreased, while plasma glucose contents, activities of glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) increased. Liver glycogen content significantly increased from $6.6\%\;to\;10.4\%$ . A large amount of hemosiderin was observed in the splenic tissue. During the recovery period, RBC number, hematocrit, hemoglobin concentration, GOT and GPT activities were returned to the normal status. Histological status of liver tissue was returned to the normal, but liver glycogen content was not recovered. During the recovery period, spleen melanin-macrophages temporarily increased, but subsequently decreased to the normal status.

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  10. [국내논문]   The Occurrence of a Dinoflagellate Gymnodinium catenatum From Chinhae Bay, Korea   피인용횟수: 2

    KIM Hak Gyoon (National Fisheries Research & Development Agency ) , MATSUOKA Kazumi (Department of Geology, Faculty of Liberal Arts, Nagasaki University ) , LEE Sam Geun (National Fisheries Research & Development Agency ) , AN Kyoung Ho (National Fisheries Research & Development Agency)
    한국수산학회지 = Journal of the Korean Fisheries Society v.29 no.6 ,pp. 837 - 842 , 1996 , 0374-8111 ,

    초록

    The resting cyst of Gymnodinium catenatum was found in the surface sediments collected from Chinhae Bay in October, 1991. This is the first record of the species in the Korean waters. The relative abundance was low with the maximum of $1.7\%$ . The colonial motile form consisting of four cells was observed in 1992 from the excystment of the cyst collected from the Wonmun Bay in Chinhae Bay. No blooms caused by G. catenatum has been observed in Chinhae Bay during the survey.

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