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Asian-Australasian journal of animal sciences 24건

  1. [국내논문]   Association of SNP Marker in IGF-I and MYF5 Candidate Genes with Growth Traits in Korean Cattle   피인용횟수: 6

    Chung, E.R. (Department of Biotechnology, College of Life Science and Natural Resources, Sangji University ) , Kim, W.T. (Department of Biotechnology, College of Life Science and Natural Resources, Sangji University)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1061 - 1065 , 2005 , 1011-2367 ,

    초록

    Growth rate is one of the economically important quantitative traits that affect carcass quantity in beef cattle. Two genes, bovine insulin-like growth factor I (IGF-I) and myogenic factor 5 (MYF5), were chosen as candidate genes for growth traits due to their important role in growth and development of mammals. The objectives of this study were to determine gene-specific single nucleotide polymorphism (SNP) markers of the IGF-I and MYF5 positional candidate genes and to investigate their associations with growth traits in Korean cattle. Genotyping of the SNP markers in these candidate genes was carried out using the single strand conformation polymorphism (SSCP) analysis. The frequencies of A and B alleles were 0.72 and 0.28 for IGF-I gene and 0.39 and 0.61 for MYF5 gene, respectively, in Korean cattle population examined. The gene-specific SNP marker association analysis indicated that the SNP genotype in IGF-I gene showed a significant association (p

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  2. [국내논문]   Estimation of the Cumulative Power of Discrimination in Haimen Chicken Populations with Ten Microsatellite Markers   피인용횟수: 1

    Olowofeso, O. (Department of Animal Genetics, Breeding and Reproduction, Yangzhou University ) , Wang, J.Y. (Department of Animal Genetics, Breeding and Reproduction, Yangzhou University ) , Shen, J.C. (Department of Animal Genetics, Breeding and Reproduction, Yangzhou University ) , Chen, K.W. (Institute of Poultry Science ) , Sheng, H.W. (Department of Animal Genetics, Breeding and Reproduction, Yangzhou University ) , Zhang, P. (Department of Animal Genetics, Breeding and Reproduction, Yangzhou University ) , Wu, R. (Department of Animal Genetics, Breeding and Reproduction, Yangzhou University)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1066 - 1070 , 2005 , 1011-2367 ,

    초록

    To estimate the cumulative power of discrimination (CPD) existing within Haimen chicken populations in China, we isolated a total of 252 genomic DNAs from four chicken populations (Rugao, Jiangchun, Wan-Nan and Cshiqishi) through a saturated salt procedure. All the genomic DNAs were used in a polymerase chain reaction (PCR) with ten microsatellite markers. Amplified PCR-products with the selected markers were separated on a 12% polyacrylamide gel with pBR322DNA/MspI used as internal standard marker. Genetic diversity indices including mean allele number among loci, unbiased heterozygosity ( $h_i$ ) within locus, effective number of alleles ( $N_e$ ) and polymorphism information content (PIC) as well as the unbiased average heterozygosity (H) among loci in the populations were calculated using the generated allele frequencies by each marker. The mean allele number for all loci ranged between 4.00 ${\pm}$ 0.33 (Rugao) to 4.90 ${\pm}$ 0.48 (Cshiqishi) and across populations for all loci was 4.60 ${\pm}$ 0.20, while (H) ranged from 0.65 ${\pm}$ 0.03 (Rugao) to 0.69 ${\pm}$ 0.03 (Jiangchun) among loci and across populations, (H) was 0.67 ${\pm}$ 0.01. The generated unbiased average heterozygosity among loci in each population was integrated to the global formula of CPD and the result demonstrated that the CPD within the four Haimen chicken populations was 98.75%.

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  3. [국내논문]   Microsatellite Polymorphism and Genetic Relationship in Dog Breeds in Korea  

    Cho, G.J. (College of Veterinary Medicine, Kyungpook National University)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1071 - 1074 , 2005 , 1011-2367 ,

    초록

    Microsatellite polymorphism and their genetic relationships were estimated using genotype information of 183 dogs from 11 microsatellite loci. The breeds include the indigenous Korean breeds Jindo dog (30), Poongsan dog (20) and Miryang dog (44) together with Chihauhau dog (31) and German Shepherd dog (58). Jindo dogs showed the highest expected heterozygosity (0.796 ${\pm}$ 0.030) and polymorphic information contents (0.755) in all populations. The phylogenetic analysis showed the existence of two distinct clusters supported by high bootstrap values: the Korean native dogs and other dogs. They clearly show that Poongsan dog and Miryang dog are closely related to each other when compared with Jindo dog. Microsatellite polymorphism data was shown to be useful for estimating the genetic relationship between Korean native dogs and other dog breeds, and also can be applied for parentage testing in those dog breeds.

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  4. [국내논문]   Genetic Parameters and Responses in Growth and Body Composition Traits of Pigs Measured under Group Housing and Ad libitum Feeding from Lines Selected for Growth Rate on a Fixed Ration  

    Nguyen, Nguyen Hong (School of Veterinary Science, University of Queensland ) , McPhee, C.P. (Animal Research Institute, Queensland Department of Primary Industries and Fisheries)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1075 - 1079 , 2005 , 1011-2367 ,

    초록

    The main objective of this study is to examine genetic changes in growth rate and carcass composition traits in group housed, ad libitum fed pigs, from lines of Large White divergently selected over four years for high and low post-weaning daily gain on a fixed but restricted ration. Genetic parameters for production and carcass traits were also estimated by using average information-restricted maximum likelihood applied to a multivariate individual animal model. All analyses were carried out on 1,728 records of group housed ad libitum fed pigs, and include a full pedigree of 5,324 animals. Estimates of heritability (standard errors in parentheses) were 0.11 (0.04) for lifetime daily liveweight gain (LDG), 0.13 (0.04) for daily carcass weight gain (CDG) and 0.28 (0.06) for carcass backfat (CFT). Genetic correlations between LDG and CDG were highly positive and between LDG and CFT negative, suggesting that selection for lifetime daily gain under commercial conditions of group housing with ad libitum feeding would result in favourable improvement in carcass traits. CFT showed negative genetic correlations with CDG. Correlated genetic responses evaluated as estimated breeding values (EBVs) were obtained from a multivariate animal model-best linear unbiased prediction analysis. After four years of divergent selection for 6 week post-weaning growth rate on restricted feeding, pigs performance tested on ad libitum feeding in groups exhibited changes in EBVs of 6.77 and -9.93 (g/d) for LDG, 4.25 and -7.08 (g/d) for CDG, and -1.42 and 1.55 (mm) for CFT, in the high and low lines, respectively. It is concluded that selection for growth rate on restricted feeding would significantly improve genetic performance and carcass composition of their descendants when group housed and ad libitum fed as is a common commercial practice.

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  5. [국내논문]   cDNA Microarray Analysis of the Gene Expression Profile of Swine Muscle   피인용횟수: 2

    Kim, Chul Wook (Department of International Livestock Industry, Jinju National University ) , Chang, Kyu Tae (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology ) , Hong, Yeon Hee (Department of Food Production Science, Faculty of Agriculture, Shinshu University ) , Jung, Won Yong (Department of International Livestock Industry, Jinju National University ) , Kwon, Eun Jung (Department of International Livestock Industry, Jinju National University ) , Cho, Kwang Keun (Department of International Livestock Industry, Jinju National University ) , Chung, Ki Hwa (Department of International Livestock Industry, Jinju National University ) , Kim, Byeong Woo (Division of Applied Life Science Institute of Agriculture & Life Sciences, Gyeongsang National University ) , Lee, Jung Gyu (Division of Applied Life Science Institute of Agriculture & Life Sciences, Gyeongsang National University ) , Yeo, Jung Sou (Institute of Biotechnology Yeungnam University ) , Kang, Yang Su (Gyeongsangnam-do Agricultural Research and Extension Servies ) , Joo, Young Kuk (Institute of Gyeongnam Province Advanced Swine Research)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1080 - 1087 , 2005 , 1011-2367 ,

    초록

    By screening specific genes related to the muscle growth of swine using cDNA microarray technology, a total of 5 novel genes (GF (growth factor) I, II, III, IV and V) were identified. Results of southern blotting to investigate the number of copies of these genes in the genome of swine indicated that GF I, GF III, and GF V existed as one copy and GF II, and GF IV existed as more than two copies. It was suggested that there are many isoforms of these genes in the genome of swine. Also, results of northern blotting to investigate whether these genes were expressed in grown muscle, using GF I, III, and V indicated that all the genes were much more expressed in the muscle of swine with body weight of 90 kg. Expression patterns of these genes in other organs, namely muscle and propagation and fat tissues, were investigated by extracting RNA from the tissues. These genes were not expressed in the propagation and fat tissues, but were expressed in the muscle tissue. To determine the mechanism of muscle growth, further studies should be preceded using the 3 specific genes related to muscle growth, that is GF I, III, and V.

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  6. [국내논문]   Complex Segregation Analysis of Categorical Traits in Farm Animals: Comparison of Linear and Threshold Models  

    Kadarmideen, Haja N. (Statistical Animal Genetics Group, Institute of Animal Science, Swiss Federal Institute of Technology ETH Zentrum ) , Ilahi, H. (Statistical Animal Genetics Group, Institute of Animal Science, Swiss Federal Institute of Technology ETH Zentrum)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1088 - 1097 , 2005 , 1011-2367 ,

    초록

    Main objectives of this study were to investigate accuracy, bias and power of linear and threshold model segregation analysis methods for detection of major genes in categorical traits in farm animals. Maximum Likelihood Linear Model (MLLM), Bayesian Linear Model (BALM) and Bayesian Threshold Model (BATM) were applied to simulated data on normal, categorical and binary scales as well as to disease data in pigs. Simulated data on the underlying normally distributed liability (NDL) were used to create categorical and binary data. MLLM method was applied to data on all scales (Normal, categorical and binary) and BATM method was developed and applied only to binary data. The MLLM analyses underestimated parameters for binary as well as categorical traits compared to normal traits; with the bias being very severe for binary traits. The accuracy of major gene and polygene parameter estimates was also very low for binary data compared with those for categorical data; the later gave results similar to normal data. When disease incidence (on binary scale) is close to 50%, segregation analysis has more accuracy and lesser bias, compared to diseases with rare incidences. NDL data were always better than categorical data. Under the MLLM method, the test statistics for categorical and binary data were consistently unusually very high (while the opposite is expected due to loss of information in categorical data), indicating high false discovery rates of major genes if linear models are applied to categorical traits. With Bayesian segregation analysis, 95% highest probability density regions of major gene variances were checked if they included the value of zero (boundary parameter); by nature of this difference between likelihood and Bayesian approaches, the Bayesian methods are likely to be more reliable for categorical data. The BATM segregation analysis of binary data also showed a significant advantage over MLLM in terms of higher accuracy. Based on the results, threshold models are recommended when the trait distributions are discontinuous. Further, segregation analysis could be used in an initial scan of the data for evidence of major genes before embarking on molecular genome mapping.

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  7. [국내논문]   Milk Yield and Immune Response of Periparturient and Early Lactation Friesian Cows Fed Diets Supplemented with a High Level of Amino-acid Chelated Chromium   피인용횟수: 1

    Terramoccia, S. (C.R.A. Istituto Sperimentale per la Zootecnia ) , Bartocci, S. (C.R.A. Istituto Sperimentale per la Zootecnia ) , Lillini, E. (Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1098 - 1104 , 2005 , 1011-2367 ,

    초록

    The trial was carried out on twenty-one Friesian cows at the end of eight months gestation, nine multiparous and twelve primiparous; allocated into three groups (1 control, 2 and 3 experimental). The same diet was administred to all three groups before partum (12.8 kg DM/head/day) and after partum (18.8 kg DM/head/day). The cows in groups 2 and 3 received two different daily quantities of amino-acid chelated chromium (0.6 and 1.2 mg Cr/kg DM) from 4 weeks prior to presumed parturition to 6 weeks after. The milk yield control was carried out at 15, 30, 42 and 60 days. All animals were immunised two weeks prior to the presumed parturition and two weeks after with the following antigens: ovalbumin and brucellergene. Blood samples were collected weekly to monitor humoral and cell-mediated immune responses. When analysing the results of antibody immunity (ovalbumin) in the sixth blood collection both treated groups significantly increased compared to group 1 (0.5230 and 0.4536 vs. 0.1812 OD; p $\gamma$ -IFN indicator, with the lower dose. A significant increase of the milk yield resulted at both 42 and 60 days with the highest level of chromium.

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  8. [국내논문]   Dietary Fatty Acid Supplementation during Transitional Period Increases Milk Production in Dairy Cows  

    Nagao, K. (Laboratory of Animal Feeds and Production, University Farm, Graduate School of Bioagricultural Sciences Nagoya University ) , Takahara, Y. (Gifu Prefectural Livestock Research Institute ) , Asai, H. (Gifu Prefectural Livestock Research Institute ) , Hayashi, N. (Gifu Prefectural Livestock Research Institute ) , Kitayama, T. (Gifu Prefectural Livestock Research Institute ) , Yoshimura, Y. (Gifu Prefectural Livestock Research Institute ) , Yokota, H. (Laboratory of Animal Feeds and Production, University Farm, Graduate School of Bioagricultural Sciences Nagoya University ) , Kita, Kazumi (Laboratory of Animal Feeds and Production, University Farm, Graduate School of Bioagricultural Sciences Nagoya University)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1105 - 1109 , 2005 , 1011-2367 ,

    초록

    The effect of dietary fatty acid supplementation on milk production, milk composition and plasma constituents in dairy cows was examined. Dietary fatty acids including mainly palmitic acid and stearic acid were given to cows from 2 weeks before and 8 weeks after parturition. Weekly gain of daily milk production was increased gradually during early lactation period and reached a plateau at 4 weeks after parturition. Weekly gain of daily milk production in lactating cows received dietary fatty acids was significantly higher than that of cows given a control diet alone. Although milk lactose concentration was slightly decreased by dietary fatty acid supplementation, milk fat and protein were not significantly influenced by dietary fatty acid supplementation. Dietary fatty acids did not affect plasma concentrations of triglyceride, non-esterified fatty acids, total cholesterol, high-density lipoprotein cholesterol and glucose during postpartum. It is suggested that dietary fatty acid supplementation has the potency to enhance energy balance and improve milk yield without any adverse effects on milk composition.

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  9. [국내논문]   Effect of Lactic Acid Producing Bacteria on the Performance of Male Crossbred Calves Fed Roughage Based Diet  

    Ramaswami, N. (Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute ) , Chaudhary, L.C. (Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute ) , Agarwal, N. (Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute ) , Kamra, D.N. (Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1110 - 1115 , 2005 , 1011-2367 ,

    초록

    To study the effect of feeding lactic acid producing bacteria on the performance of cattle calves, twenty four, day old male crossbred cattle calves (Bos indicus ${\times}$ Bos taurus), were distributed into two groups of 12 animals each. The animals were fed on calf starter containing wheat bran and green berseem ad libitum and milk as per requirement upto 8 weeks of age. The diet of calves of Group 2 was supplemented with 500 ml culture of Lactobacillus acidophilus-15. Total duration of the experiment was 31 weeks. There was no significant difference in intake and digestibility of dry matter (DM), organic matter (OM), neutral detergent fibre (NDF), acid detergent fibre (ADF) and crude protein (CP) between the groups. The rumen pH, protozoa numbers, concentration of volatile fatty acids (VFA), ammonia nitrogen ( $NH_3-N$ ), trichloroacetic acid precipitable nitrogen (TCA-ppt N) and activity of microbial enzymes (carboxymethylcellulase, xylanase, amylase and protease) were not affected due to probiotic supplementation. Average live weight gain of the calves was improved (about 10%) and feed:gain ratio was reduced (about 5%) in the animals given Lactobacillus culture. The data indicated that crossbred calves could be reared on a diet devoid of cereal grain and addition of Lactobacillus culture in the diet resulted in an added advantage in growth performance of the animals.

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  10. [국내논문]   Influence of Fiber Content and Concentrate Level on Chewing Activity, Ruminal Digestion, Digesta Passage Rate and Nutrient Digestibility in Dairy Cows in Late Lactation   피인용횟수: 2

    Tafaj, M. (University of Hohenheim, Institute of Animal Nutrition ) , Kolaneci, V. (University of Hohenheim, Institute of Animal Nutrition ) , Junck, B. (University of Hohenheim, Institute of Animal Nutrition ) , Maulbetsch, A. (University of Hohenheim, Institute of Animal Nutrition ) , Steingass, H. (University of Hohenheim, Institute of Animal Nutrition ) , Drochner, W. (University of Hohenheim, Institute of Animal Nutrition)
    Asian-Australasian journal of animal sciences v.18 no.8 ,pp. 1116 - 1124 , 2005 , 1011-2367 ,

    초록

    The influence of fiber content of hay (low-fiber 47% NDF and high-fiber 62% NDF of DM) and concentrate level (high 50% and low 20% of ration DM) on chewing activity, passage rate and nutrient digestibility were tested on four restrict-fed (11.1 to 13.7 kg DM/d) Holstein cows in late lactation. Aspects of ruminal fermentation and digesta particle size distribution were also investigated on two ruminally cannulated (100 mm i.d.) cows of the same group of animals. All digestion parameters studied were more affected by the fiber content of the hay and its ratio to non structural carbohydrates than by the concentrate level. Giving a diet of high-fiber (62% NDF) hay and low concentrate level (20%) increased chewing activity but decreased solid passage rate and total digestibility of nutrients due to a limited availability of fermentable OM in the late cut fiber rich hay. A supplementation of high-fiber hay with 50% concentrate in the diet seems to improve the ruminal digestion of cell contents, whilst a depression of the ruminal fiber digestibility was not completely avoided. Giving a diet of low-fiber (47% NDF) hay and high concentrate level (50%) reduced markedly the chewing and rumination activity, affected negatively the rumen conditions and, consequently, the ruminal digestion of fiber. A reduction of the concentrate level from 50 to 20% in the diet of low-fiber hay improved the rumen conditions as reflected by an increase of the ruminal solid passage rate and of fiber digestibility and in a decrease of the concentration of large particles and of the mean particle size of the rumen digesta and of the faeces. Generally, it can be summarised that, (i) concentrate supplementation is not a strategy to overcome limitations of low quality (fiber-rich) hay, and (ii) increase of the roughage quality is an effective strategy in ruminant nutrition, especially when concentrate availability for ruminants is limited.

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