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권호별목차 / 소장처보기

H : 소장처정보

T : 목차정보

Journal of plant biology = 식물학회지 10건

  1. [국내논문]   Sequence Homologies of GTP-binding Domains of Rab and Rho between Plants and Yeast/Animals Suggest Structural and Functional Similarities  

    Lee, Ji-Yeon ; Lee, Dong-Hee
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 85 - 92 , 1996 , 1226-9239 ,

    초록

    Small GTP-binding proteins are divided into three major group: Ras, Rho and Ypt/Rab. They have the conserved regions designed G1 to G5 that are critical in GDP/GTP exchange, GTP-induced conformational change and GTP hydrolysis. We isolated and characterized genomic DNA or cDNAfragments encoding G1 to G3 domains of small GTP-binding protein Rab and Rho from several plant species using two different PCR-based cloning strategies. Seven rab DNA fragments were isolated from 4 different plants, mung-bean, tobacco, rice and pepper using two degenerate primers corresponding to the GTP-binding domain G1 and G3 in small GTP-binding proteins. The amino acid sequences among these rab DNA fragments and other known small GTP-binding proteins shows that they belong to the Ypt/Rab family. Six rho DNA fragments were isolated from 5 different plants, mung-bean, rice, Arabidopsis, Allium and Gonyaulax using the nested PCR method that involves four degenerate primers corresponding to the GTP-binding domain G1, G3 and G4. The rho DNA fragments cloned show more than 90% homology to each other. Sequence comparison between plant and other known Rho family genes suggests that they are closely related (67 to 82% amino acid identity). Sequence analysis and southern blot analysis of rab and rho in mung-bean suggest than thses genes are encoded by multigene family in mung-bean.

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  2. [국내논문]   Improved in vitro Regeneration of Potato (Solanum tuberosum cv. Superior) Transformed by Agrobacterium Expressing $\beta-Glucuronidase$  

    Park, Yoon-Kyung ; Park, Gene-Sue ; Yang, Young-Ki ; Cheong, Hyeon-Sook
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 93 - 98 , 1996 , 1226-9239 ,

    초록

    In order to enhance the system of potato transformation and further regeneration, potato was transformed using the Agrobacterium tumefaciens harboring $\beta$ -glucuronidase (GUS) gene. We found that a series fo modified medium ttained 100% shoot regeneration within 5 weeks after the preincubated explants on stage I medium were infected with Agrobacterium. Callus appeared at the cut edges of stem segments on stage II medium, mainly at the basal parts. Some explants started to form shoots after two to three weeks on stage III medium containing kanamycin (50 mg/L). When transferred to MS medium containing 200 mg/L kanamycin, 81% of the transformed shoots formed roots at the cut edge of the plantlets. In contrast, untrasformed shoots never rooted and became yellowish after few weeks under the same conditions. Southern and northern analysis indicated in vitro shoot regeneration on the callus derived from the potato explants, which were incubated with Agrobacteria. The regeneration cycle was shortened after the transformatin and finally the transformation efficiency was highly enhanced.

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  3. [국내논문]   Immunocytolocalization of Cell Wall Peroxidase and Other Wall Antigens from Maize Seedlings  

    Kim, Sung-Ha
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 99 - 105 , 1996 , 1226-9239 ,

    초록

    Immunocytochemistry utilizes the specificity of the antigen-antibody reaction to localize specific antigens in cells or cellular organelles. Here we report the use of monoclonal antibodies, in conjunction with gold-labeled second antibodies to study the ultrastructural localization and tissue distribution of the Mr 98, 000 anionic peroxidase and other wall antigens. The antibody specific for this wall peroxidase, mWP3, labeled mainly the cell wall area. At the tissue level, the Mr 98, 000 peroxidase is located predominantly in the leaf mesophyll, internal coleoptile and sieve elements, but not in the root, as assayed with these procedures. The coleoptile walls were less heavily stained than the walls of leaf mesophyll cells. At the subcellular level, it is localized mainly in intercellular regions of the cell walls. A similar staining pattern was revealed by mWP19, one of anti- $\beta$ glucosidase antibody, though it looked less heavily stained than one with mWP3. In order to serve as a control wall staining using IgM monoclonal antibodies, mWP18 was used. Most of the label is localized over wall regions of cells of the young leaf mesophyll and coleoptile.

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  4. [국내논문]   The Stimulation of Arginine Decarboxylase Activity by alpha-Difluoromethyl$ Ornithine in Tobacco Suspension Cultured Cells   피인용횟수: 1

    Lee, Sun-Hi ; Kim, Yong-Bum ; Lee, Myeong-Min ; Park, Ki-Young
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 107 - 112 , 1996 , 1226-9239 ,

    초록

    To study the compensatory aspect of putrescine biosynthetic enzyme n tobacco suspension cultured cells, we examined the contents of the cellular polyamines and the activities of arginine decarboxylase (ADC, EC 4.1.1.19) and ornithine decarboxylase (ODC, EC 4.1.1.17) in the tobacco suspension cells treated with $\alpha$ -difluoromethyl arginine (DFMA) or $\alpha$ -difluoromethyl ornithine (DFMO). In the untreated cells, the content of the cellular putrescine was decreased during the first 3 hours and then subsequently increased. However, the content of the cellular spermidine and spermine remained constant during the incubation time. While ADC activity increased after 6 hours, ODC activity decreased following the rapid increase until 6 hours. DFMA induced the decrease in the contents of putrescine and spermidine, and the increase in that of spermine. It also caused the inhibition of ADC and ODC activities throughout the incubation time. DFMO produced the stimulation of ADC activity about 2 times of untreated cells and the decrease in the content of putrescine about 50% of them at 12 hour. The application of putrescine or cycloheximide prevented the increase of ADC activity by DFMO but that of actinomycin-D did not show any detectable effect. The stimulation of ADC activity by DFMO in tobacco suspension cultured cells was probably due to the enhancement of de novo synthesis for ADC protein, which might be regulated in the translation step by the content of the cellular putrescine.

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  5. [국내논문]   The Role of $Ca^{2+}$ in Retardation Effects of Benzyladenine on the Senescence of Wheat (Triticum aestivum L.) Leaves  

    Hong, Kee-Jong ; Jin, Chang-Duck ; Hong, Young-Nam
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 113 - 121 , 1996 , 1226-9239 ,

    초록

    The role of Ca2+ on benzyladenine (BA)-induced senescence retardation in mature wheat (Triticum aestivum L.) primary leaves was investigated. When an extracellular calcium chelator, ethylene glycol-bis-( $\beta$ -aminoethylether)-N, N'-tetraacetic acid (EGTA) together with BA, was applied to senescing leaves for 4 days of dark incubation, the content of chlorophyll and soluble protein decreased rapidly. And, the content of malondialdehyde (MDA), known to be a degradation product of membrane lipids, increased compared with the BA alone control. The BA-EGTA combination also caused the stimulation of protease and RNase activity and a rapid loss of catalase activity owing to the decling of BA effects. In the case of treatment with only intracellular calcium antagonist 3, 4, 5-trimethoxybenzoic acid 8-(diethylamino) octyl ester (TMB-8) without the BA addition, the chlorophyll content at day 4 after dark incubation decreased in paralled with the increasing concentration of the antagonist. In addition, the chlorophyll content at 10-5 M calcium ionophore A23187 treatment in the absence of BA was similar to that of the BA alone treatment. These results suggest that calcium may mediate the retardation effect of BA on leaf senescence by acting as a second messenger and that the calcium input from cell organelles, as well as the calcium inflow from intercellular spaces and cell walls, may be involved in modulating cytosolic calcium levels related to BA action.

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  6. [국내논문]   Immunocytochemical Localization of Storage Protein in Pea (Pisum sativum) Cotyledon  

    Yu, Seong-Cheol ; Lee, Chang-Seob ; Kim, Woo-Kap
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 123 - 126 , 1996 , 1226-9239 ,

    초록

    The pattern of seed storage protein, vicilin, deposition and site of intracellular localization was examined in cotyledon cells of pea (Pisum sativum) seed using the immunocytochemical methods. The vicilin was confined to the cisternae fo the rough endoplasmic reticulum and dictyosome as well as protein granules newly formed in rough endoplasmic reticulum. Vacuolar protein deposites and protein bodies were also labelled by gold particles. After small protein bodies were formed in the rough endoplasmic reticulum, they were transported to large protein bodies and then fused together. Electron dense protein granule, elaborated in the dictyosome, appears to be transported from dictyosome to protein body. A few unlabelled protein granules seem to be accumulated in other type of proteins than vicilin.

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  7. [국내논문]   Somatic Embryogenesis and Plant Regeneration from Immature Zygotic Embryo Culture in Pepper (Capsicum annuum L.)   피인용횟수: 2

    Jo, Jeong-Yon ; Choi, Eun-Young ; Choi, Dong-Su ; Lee, Kwang-Woong
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 127 - 135 , 1996 , 1226-9239 ,

    초록

    An efficient system of somatic embryogenesis was established for the red pepper plant (Capsicum annuum L. cv. Nokkwang) usign immature zygotic embryos. The size of the immature zygotic embryos and the concentrations of 2, 4-D and sucrose were found to be critical. Somatic embryos were induced via callus or directly from explants and regenerated into plantlets successfully. When zygotic embryos 1~2 mm long were cultured on the modified Murashige-Skoong (MS) medium supplemented with 2 mg/L 2, 4-D for 3 weeks in the dark, somatic embryos were induced directly from the apical region of zygotic embryos with the highest frequency being approximately 90%. To mature the somatic embryos, ABA and an ethylene inhibitor AgNO3 were used. The highest frequency of shoot regeneration (25% in each) resulted at 2 $\mu$ M ABA or 20 $\mu$ M AgNO3 treatment at rates 3.7 and 1.6 times control, respectively. Shoots developed mainly from the cotyledonary node on CoCl2-containing medium, and from the upper side of cotyledon on medium containing AgNO3 while the embryos on the control medium produced shoots from both the cotyledonary node and the upper region of cotyledons both at frequencies of 50%. Indirect somatic embryogenesis via callus was induced at an efficiency of approximately 10% with zygotic embryos 3~4 mm long cultured on MS medium containing 5~10 mg/L, 2, 4-D for 5~7 weeks under a continuous light condition. The plants regenerated from the somatic embryos were morphologically normal. Using scanning electron microscopy, the direct and indirect somatic embryogeneses were observed to follow the globular, heart and torpedo stages, similar to zygotic embryogenesis. Also, suspensors appeared in the early globular and ovoid-shaped late globular embryos during indirect somatic embryogenesis.

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  8. [국내논문]   Microstructure of Perigynium and Achene Surfaces of Carex sect. Sideroxtictae in Korea   피인용횟수: 1

    Oh, Yong-Cha
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 137 - 144 , 1996 , 1226-9239 ,

    초록

    Scanning electron microscope revealed several taxonomically useful characters in the perigynium and achene epidermis of sect. Siderosticatae (Carex siderosticta, C. ciliatomarginata and C. okamotoi). Mocroscopic characters such as perigynium shape, hair presence or absence, beak shape, nodule situation in perigynium, achene shape, cell wall and silica cone in achene were useful in Carex at the species levels. A key was presented on the basis of features obtained from the examinations.

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  9. [국내논문]   Indigofera grandiflora (Leguminosae), a New Species from Korea  

    Choi, Byoung-Hee
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 145 - 148 , 1996 , 1226-9239 ,

    초록

    A new species, Indigofera grandiflora B. Choi et S. Cho, was described from sourthern Korea. The new species is related to I. kirilowii and I. koreana in the Far East. However, the species differs from these two species in having larger flowers and leaflets and hairiness on leaflets. The new species is also allied to I. decora in southest China and Japan, but clearly distinguished from the latter by its larger flowers, shorter inflorescences, and hairiness on leaflets.

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  10. [국내논문]   Floristic Characterization of the Temperate Oak Forests in the Korean Peninsula Using High-rank Taxa   피인용횟수: 3

    Kim, Jong-Won
    Journal of plant biology = 식물학회지 v.39 no.2 ,pp. 149 - 159 , 1996 , 1226-9239 ,

    초록

    The order of Rhododendro-Quercetalia mongolicae representing temperate oak forests in Korea was characterized in terms of presence of taxa of different rank. 140 releves were analysed for percentage contribution of each species, genera and families in syntaxa under consideration as well as extracton of diagnostic genera and families for syntaxa by an ordination technique. The Rhododendro-Quercetalia is characterized by high diversity of tree and shrub species contributing 40% of the total floristic composition as well as by a high contribution of the genus Rhododendron and the absence of the genus Fagus, characteristic of the QuercoFagetea sensu lato. The character families for the Rhododendron and Quercus include Liliaceae and Compositae, whereas Acer, Carex, Viola, Rhododendron and Quercus are the most common among genera. Rhododendron and Quercus are regarded as the transgressive cheractergenera, whereas the families of Pinaceae and Ericaceae are considered companions (in sense of the terminology of the Braun-Blanquet syntaxonomy) for the order. Family appeared to be an inadequate rank for diagnoses of alliances and suballiances. On the other hand, genus was found to be the most effective rank in differentiating the alliances and suballiances. The Lindero-Quercenion shares the same character-genera with the order Rhododendro-Quercetalia. Character genera of the suballiances Callicarpo-quercenion are Carpinus, Styrax, Smilax and Callicarpa, and those of the Pino-Quercion list Euonymus, Saussurea and Tilia.

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