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H : 소장처정보

T : 목차정보

Journal of plant biology = 식물학회지 11건

  1. [국내논문]   Multiplication and Transformation of Medicinal Plants for Production of Useful Secondary Metabolites II. Establishment of Hairy Root Cultures of Centella asiatica   피인용횟수: 1

    Paek, Yun-Woong ; Hwang, Sung-Jin ; Park, Don-Hee ; Hwang, Baik
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 161 - 166 , 1996 , 1226-9239 ,

    초록

    The hairy root cultures of Centella asiatica were established by infection leaf explants with Agrobacterium rhizogenes A4, 15834 in 1/2 Murashing and skoog liquid medium supplemented with 50 $\mu$ M acetosyringone. The induced hairy roots were subjected to paper electrophoresis for the detection of opine and opine-positive clones which were considered to have been transformed. Five hairy root clones were selected according to the different bacterial strains used, growth rate and pattern. Among media tested, MS basal medium substituted phosphate concentration by 2.5mM K2HPO4 showed the highest growth rate in the dark condition.

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  2. [국내논문]   Plant Regeneration via Organogenesis from Seed Explants in Red Pepper (Capsicum annuum L.)  

    Lee, Kwang-Woong
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 167 - 172 , 1996 , 1226-9239 ,

    초록

    Efficient plant regeneration has been achieved via organogenesis in the red pepper plant (Capsicum annum L.). Shoots were induced from seed explants of cultivar 'Friendship' on Murashige and Skoog's (MS) basal medium supplemetned with; NAA or IAA, and BAP or zeatin. Seed explants on the medium supplemented with 0.1-0.3 mg/L IAA and 2-5 mg/L zeatin for 2 weeks vigorously formed normal shoots in more than 90% of the explants. When these were transferred to MS medium containing 0.5-1.0 mg/L GA, 90-100% of the shoots have elongated within 1-2 weeks. The elongated shoots rooted in media supplemented with 0.3 mg/L NAA. It was revealed that this method is very rapid and efficient regeneration system for red pepper and regenerated plants can be obtained after only 5-6 weeks of culture.

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  3. [국내논문]   Purification and Characterization of a Novel 21 kD Calcium Binding Protein from Dunaliella salina  

    Ko, Jae-Heung ; Lee, Sun-Hi
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 173 - 177 , 1996 , 1226-9239 ,

    초록

    A novel calcium binding protein (CaBP) was purified to electrophoretic homogeneity from Dunaliella salina. In the course of purification experiment, this CaBP was identified as a monomer and its molecular weight was about 21 kDand isoelectric point (pI) value was about 4.1 using isoelectrofocusing. This CaBP was able to bind Ca2+ even in the pressence of an excess MgCl2 and KCI both in solution. In the SDS-PAGE, the Ca2+-bound form was slower than the Ca2+-free form in the nondenaturing PAGE. This means that the CaBP undergoes conformational change in the Ca2+-bound condition. Furthermore, UV absorption spectrum and fluorescence intensity of this CaBP was investigated. UV absorption peak was appeared at about 258 nm and decreased somewhat in Ca2+-bound condition. In the measurement of fluorescence, maximum intensity was appeared at 303 nm and decreased in Ca2+-bound state, similarly as UV absorption spectrum. These show distinct changes upon Ca2+-binding, which indicate of structural and/or dynamic changes largely reminiscent of other members of the EF-hand Ca2+-binding protein family.

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  4. [국내논문]   Effective Combination of Agrobacterium tumefaciens Strains and Ti Plasmids for the Construction of Plant Vector System   피인용횟수: 1

    Kim, Mi-Suk ; Park, Jeong-Du ; Eum, Jin-Seong ; Sim, Woong-Seop
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 179 - 184 , 1996 , 1226-9239 ,

    초록

    The purpose of this study is to obtain the most efficient combination of Agrobacterium tumefaciens strains and Ti plasmids for the construction of dicotyledonous plant vector system. Ti plasmid-curing A. tumefaciens A136 and KU12C3 were transformed with four kinds of Ti plasmids, pTiBo542, pTiA6, pTiKU12 and pTiAch5, respectively. The stems of 28 species of dicotyledonous plants were then inoculated with these transformants and examined for crown gall formation. The different combination of A. tumefaciens strains and Ti plasmids showed quite a difference in terms of the crown gall formation. Agrobacterium strins A136 and KU12C3 have a same plant host range in case that both strains harour the same kind of Ti plasmid, pTiBo542 or pTiAch5. However, the above-mentioned both strains have quite different host range in the event of containing the same Ti plasmid, pTiKU12 or pTiA6. In case that KU12C3 contains pTiA6 or pTiKU12, this strain has a wider plant host range than A136. The plant host range of pTiBo542 is the widest, followed by pTiA6, pTiKU12 and pTiAch5. Twelve plants among 28 tested plants are not transformed by any virulent Agrobacterium strains used in this study. In conclusion, A. tumefaciens KU12C3 and A136 harboring pTiBo542 showed the widest host range for transforming dicotyledonous plants. Also, it was acertained that the host range of Ti plasmids is affected by chromosomal level.

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  5. [국내논문]   Detection of Genetic Variation and Gene Introgression in Potato Dihaploids Using Randomly Amplified Polymorphic DNA (RAPD) Markers   피인용횟수: 1

    Cho, Un-Haing ; Cho, Hyun-Mook ; Kim, Hei-Young
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 185 - 188 , 1996 , 1226-9239 ,

    초록

    Randomly amplified polymorphic DNAs were employed to study the genetic variation and gene introgression in potato dihaploids (2n=24) which were generated after interspecific pollination of tetraploid cultivars (2n=4X=48, Solanum tuberosum cv Irish Cobbler, Superior and Dejima) by haploid inducer clones (2n=2X=24, Solanum phureja 1.22, Hes-5 and Hes-6). Genetic variation and DNA marker segregation among dihaploids were observed. Most dihaploids contain S. tuberosum specific RAPD markers but haploid inducer-specific RAPD markers were also found in some dihaploids. Of six different arbitrary 10-mer oligonucletide primers which showed polymorphism betwen tetraploid cultivars and haploid inducers used, three generated amplification products which seemed to be derived from the S. phureja parent. Our results indicate that chromosomes of dihaploids may not be pure S. tuberosum and the dihaploids may not be produced by parthenogenesis.

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  6. [국내논문]   Cloning of Epidermis-specific cDNAS Encoding a Lipid Transfer Protein and an Aldehyde Decarbonylase from Senecio odorus  

    Pyee, Jaeho
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 189 - 195 , 1996 , 1226-9239 ,

    초록

    The major cuticular components have been shown to be synthesized in the epidermis. Therefore, cloning of epidermis-specific genes could yield information to be used to isolate and characterize the enzymes involved in the cuticle biosynthesis. A subtractive cDNA library was prepared from Senecio odorus in which epidermis-specific cDNAs were enriched. Differential screening of the library using epidermal and non-epidermal probes revealed two cDNAs. One of them designated epi425 was identified, based on the sequence homology, as a member of a new class in the LTP gene family and the other clone designated epi23 as a gene encoding an aldehyde decarbonylase. Northern blot analyses showed that epi425 and epi23 cDNAs hybridized with a transcript of about 600 and 2, 100 nucleotides, respectively, from the epidermis but not from the non-epidermal tissues. Further characterization of these clones will provide more information on the mechanism of the cuticle biosynthesis.

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  7. [국내논문]   Isolation and Characterization of a Pollen-specific cDNA Clone from Easter Lily  

    Kim, Seong-Ryong ; An, Gyu-Heung
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 197 - 202 , 1996 , 1226-9239 ,

    초록

    A pollen-specific cDNA clone, LMP50, was isolated from the mature pollen cDNA library of the Easter lily. The LMP50 transcript was highly abundant in mture pollen grains but not detectable in other organs. The LMP50 cDNA clone contains 1383 nucleotides and two open reading frames. The first codes for a peptide of 15 amino acid residues. The role of this peptide is nuclear. The second encodes a protein containing 329 amino acid residues. This protein exhibited a significant homology to human tartrate-resistant acid phosphatase and porcine uteroferrin. Both of these enzymes have been suggested to play a role in iron transport. Therefore, LMP50 may act as an iron carrier protein in mature pollen grains.

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  8. [국내논문]   Intraspecific Polymorphism and Classification of Paeonia Iactiflora Based on the Giemasa C-banding Patterns  

    Seo, Bong-Bo
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 203 - 207 , 1996 , 1226-9239 ,

    초록

    On the basis of karyotypic analysis performed by conventional staining and Giemas C-banding technique, cytological relationship was inferred for 21 lines of Paeonia lactiflora Pal. cultivated in Korea. It was very difficult to infer their organized karyotypic classification system using the composition of somatic chromosomes involving sat-chromosomes, relative length of chromosomes, arm ratio and karyotypic formulae by conventional staining. From the distribution and number of Giemsa C-bands on the chromosomes b and c, 21 lines can be subclassified into 5 groups. It seems that the karyotypic polymorphism is observed in 21 lines of cultivated P. lactiflora because peony mainly propagates by outbreeding.

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  9. [국내논문]   Cytological Evolution in the Genus Lycoris (Amarylidaceae)   피인용횟수: 1

    Tae, Kyoung-Hwan ; Ko, Sung-Chul
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 209 - 214 , 1996 , 1226-9239 ,

    초록

    Cytological characters of ten taxa of the genus Lycoris were investigated to illustrate their chromosomal evolution. The fusion theory was more appropriate than the fission theory. From the viewpoint of the fusion theory L. sanguinea group, diploids with R-shaped chromosomes was the most primitive and they might have diverged into two directions, the diploids with V+R shaped chromosomes and the triped with V+R shaped chromosomes via triploids with only R-shaped chromosomes.

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  10. [국내논문]   Cytological Modification of Sorghum Leaf Tissues Showing the Early Acute Response to Maize Dwrf Mosaic Virus   피인용횟수: 1

    Choi, Chang-Won
    Journal of plant biology = 식물학회지 v.39 no.3 ,pp. 215 - 221 , 1996 , 1226-9239 ,

    초록

    Sorghum leaf tissues showing the early acute response of systemic infection with maize dwarf mosaic virus (MDMV) strain A, contained unusual virus-induced cytological modifications including cell wall thickenings and protrusions, intercellular vesicles termed as "paramural bodies", modified plasmodesmata, abnormal plastids, and cylindrical inclusion bodies. Abnormal cell wall, some of which associated with paramural bodies, was frequently contained modified plasmodesmata. Various abnormal plastids were located within infected cells of leaf tissues showing the early acute response. The most important changes in chloroplast seen in the tissues are the presence of small vesicles, deformation of membranes, reduction in granal stack height, disappearance of osmiophilic globules and degeneration of stuctures. The cytological modification was not occurred in nucleus but a group of degenerated mitochondria with abnormal membranes attached to cylindrical inclusion bodies were observed. It was hard more or less to prove the relationship clearly between virus and cellular organelles in virus replication.plication.

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