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Journal of plant biology = 식물학회지 17건

  1. [국내논문]   Selection and Characterization of Transposon Tagging Mutants of Synechocystis sp. PCC 6803 Sensitive to High-Light and Oxidative Stresses   피인용횟수: 1

    Song, Eun-Kyeong (Department of Molecular Biology, Pusan National University ) , Zulfugarov, Ismayil S. (Department of Molecular Biology, Pusan National University ) , Kim, Jin-Hong (Department of Molecular Biology, Pusan National University ) , Kim, Eun-Ha (Department of Molecular Biology, Pusan National University ) , Lee, Woo-Sung (Department of Biological Science, Sungkyunkwan University ) , Lee, Choon-Hwan (Department of Molecular Biology, Pusan National University)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 289 - 299 , 2004 , 1226-9239 ,

    초록

    We compared several analytical tools to identify which were most applicable for the selection and characterization of specific transposon-tagged mutant strains of Synechocystis sp. PCC 6803 that are sensitive to high light and oxidative stresses. Our primary parameter was the maximum photochemical efficiency of dark-adapted cells, a very sensitive factor that can be determined in a non-destructive manner. Using this as a tool for primary selection, we identified five mutant strains with different sensitivities to photoinhibition and photooxidation. For further characterization, we obtained data describing the absorption spectra for pigment contents, the 77 K fluorescence spectra, non-photochemical quenching (as a down-regulation process), and the photosynthetic electron transfer rate. Based on these results, we were able to design a strategy for selecting mutants with specific phenotypes. Here, we also discuss the strengths and weaknesses of each selection and characterization tool.

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  2. [국내논문]   Agrobacterium-Mediated Transformation System for Large-Scale Producion of Transgenic Chinese Cabbage (Brassica rapa L. ssp. pekinensis) Plants for Insertional Mutagenesis   피인용횟수: 4

    Lee, Mi-Kyung (Graduate School of Biotechnology and Department of Horticultural Biotechnology, KyungHee University ) , Kim, Hyoung-Seok (Graduate School of Biotechnology and Department of Horticultural Biotechnology, KyungHee University ) , Kim, Jung-Sun (Brassica Genomics Team, National Institute of Agricultural Biotechnology ) , Kim, Sung-Hoon (Graduate School of East-West Medical Science, KyungHee University ) , Park, Young-Doo (Graduate School of Biotechnology and Department of Horticultural Biotechnology, KyungHee University)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 300 - 306 , 2004 , 1226-9239 ,

    초록

    In order to better utilize insertional mutagenesis and functional genomics in Chinese cabbage, we have developed an improved transformation system that more efficiently produces a large number of transgenic plants. Hypocotyl explants were inoculated with Agrobacterium tumefaciens LBA4404. This strain harbors tagging vector pRCV2, which contains a hygromycin-resistance gene, an ampicillin resistance gene, and a bacterial replication origin within the T-DNA. Transformation efficiency was highest when the explants were first co-cultivated for 3 d in a medium supplemented with 5 mg L-1 acetosyringone, then transferred to a 0.8% agar selection medium containing 10 mg L-1 hygromycin. In addition, maintaining a low pH in the co-cultivation medium was critical to enhancing transformation frequency. A total of 3369 transgenic plants were obtained, with efficiencies ranging from 2.89% to 5.00%. Southern blot analysis and T1 progeny tests from 120 transgenic plants confirmed that the transgenes were stably inherited to the next generation. We also conducted plasmid rescue and inverse PCR with some transformants, based on their phenotype, to demonstrate the applicability of T-DNA tagging in Chinese cabbage. The tagged sequences were then analyzed.

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  3. [국내논문]   Ethylene Biosynthesis in a Chilling-Sensitive Arabidopsis Mutant, chs4-2  

    Kim, Sun-A (Department of Biological Science, Ewha Womans University ) , Kim, Seong-Ki (Department of Life Science, Chung-Ang University ) , Kaufman, Peter B. (Department of Molecular, Cellular, and Developmental Biology, University of Michigan ) , Lee, June-Seung (Department of Biological Science, Ewha Womans University ) , Chang, Soo-Chul (University College, Yonsei University)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 307 - 313 , 2004 , 1226-9239 ,

    초록

    We investigated chilling-induced changes in ethylene levels in Arabidopsis to find plants with distinct patterns of ethylene production in the cold-related biosynthetic pathway. The sensitive mutants identified here included chs1-2, chs4-2, and chs6-2. Among these, plants of the chs4-2 mutant produced more ethylene than did the wild type after both were transferred from 4 $^{\cidt}C$ or 10 $^{\cidt}C$ to 22 $^{\cidt}C$ . This mutant also showed less freezing tolerance and more electrolyte leakage than the wild-type plants. Our results suggest a relationship between ethylene biosynthesis and chilling sensitivity in the mutant. To determine which of the enzymes involved in ethylene biosynthesis were induced by chilling, we tested the activities of ACC synthase and ACC oxidase in both mutant and wild-type plants, and found greater activity by ACC synthase as well as a higher ACC content in the mutants after all the plants were transferred from $^{\cidt}C$ to 22 $^{\cidt}C$ . However, ACC oxidase activity did not differ between mutant and wild-type plants in response to chilling treatment. Therefore, we conclude that chs4-2 mutants produce more ethylene than do other mutants or the wild type during their recovery from chilling conditions. Furthermore, we believe that ACC synthase is the key enzyme involved in this response.

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  4. [국내논문]   Alterations in the Photosynthetic Pigments and Antioxidant Machineries of Red Pepper (Capsicum annuum L.) Seedlings from Gamma-Irradiated Seeds   피인용횟수: 5

    Kim, Jin-Hong (Division of Radiation Application Research, Korea Atomic Energy Research Institute ) , Baek, Myung-Hwa (Division of Radiation Application Research, Korea Atomic Energy Research Institute ) , Chung, Byung-Yeoup (Division of Radiation Application Research, Korea Atomic Energy Research Institute ) , Wi, Seung-Gon (Division of Radiation Application Research, Korea Atomic Energy Research Institute ) , Kim, Jae-Sung (Division of Radiation Application Research, Korea Atomic Energy Research Institute)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 314 - 321 , 2004 , 1226-9239 ,

    초록

    To characterize the stimulatory effects of low-dose gamma radiation on early plant growth, we investigated alterations in the photosynthesis and antioxidant capacity of red pepper (Capsicum annuum L.) seedlings produced from gamma-irradiated seeds. For two cultivars (Yeomyung and Joheung), three irradiation groups (2, 4, and 8 Gy, but not 16 Gy) showed enhanced development, although Fv/Fm, the maximum photochemical efficiency of Photosystem II (PSII), did not differ significantly among any of the four groups. In contrast, values for 1/Fo - 1/Fm, i.e., a measure of functional PSII content, decreased in the irradiated groups of `Yeomyung' but increased in those of `Joheung'. Pigment analyses and enzyme activity assays revealed that irradiation altered the compositions of photosynthetic pigments (chlorophylls and carotenoids) as well as the activities of antioxidant enzymes (superoxide dismutase and glutathione reductase). However, these shifts were not directly related to the increase in early growth, although they were cultivar- and developmental stage-dependent. In addition, the effects of irradiation on the enzymatic activities measured here were at opposition between the two cultivars.

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  5. [국내논문]   Geographical Distribution of Cytotypes and Genomic Structures in Natural Populations of the Scilla scilloides Complex in Korea   피인용횟수: 1

    Choi, Hae-Woon (School of Bioscience and Biotechnology, Chungnam National University ) , Lee, Woo-Kyu (School of Bioscience and Biotechnology, Chungnam National University ) , Choi, Eun-Young (School of Bioscience and Biotechnology, Chungnam National University ) , Park, Jae-Hyun (School of Bioscience and Biotechnology, Chungnam National University ) , Bang, Jae-Wook (School of Bioscience and Biotechnology, Chungnam National University)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 322 - 329 , 2004 , 1226-9239 ,

    초록

    Cytotype distribution, B-chromosome frequency, and genomic constitution in the natural populations of the Scilla scilloides complex in Korea were analyzed. Plants with various cytotypes were found: AA (2n=16), BB (18), AAB (25), ABB (26), ABB (34), ABBB (35), BBBB (36), AABBB (43), AAABBB (51) and AAAABBBB (68). Allotetraploid AABB plants predominated with a frequency of 68.3%, and were found to distribute all over the Korean peninsula and Cheju-do. In diploids, the type AA plants distributed throughout the Korean midwest, while the type BB plants were limited to Cheju-do. Two other cytotypes, ABBB and AABBB, were found only in the southern part of the Korean peninsula including Cheju-do. Chromosomal variations, aneuploidy, and centromeric shifts were also found in the natural populations. The cytotypes AAB and AAAABBBB are reported here for the first time. B-chromosomes were found in 149 (85.6%) of 174 populations, the highest frequency being 81.8% in BB populations. The number of B-chromosomes per plant ranged from 1 to 31, and 1B plants predominated (21.0%). Subtypes, with respect to the number and composition of B-chromosomes, indicated that sexual reproduction is still prevalent in AABB populations.

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  6. [국내논문]   Defects in a Proteolytic Step of Light-Harvesting Complex II in an Arabidopsis Stay-Green Mutant, ore10, during Dark-Induced Leaf Senescence   피인용횟수: 1

    Oh, Min-Hyuk (Department of Molecular Biology, Pusan National University ) , Kim, Jin-Hong (Department of Molecular Biology, Pusan National University ) , Moon, Yong-Hwan (Department of Molecular Biology, Pusan National University ) , Lee, Choon-Hwan (Department of Molecular Biology, Pusan National University)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 330 - 337 , 2004 , 1226-9239 ,

    초록

    During dark-induced leaf senescence (DIS), the non-functional stay-green mutant ore10 showed delayed chlorophyll (Chl) degradation and increased stability in its light-harvesting complex II (LHCII). These phenomena were closely related to the formation of aggregates that mainly consisted of terminal-truncated LHCII (Oh et al., 2003). The ore10 mutant apparently lacks the protease needed to degrade the truncated LHCII. In wild-type (WT) plants, protease was found in the thylakoid fraction, but not the soluble fraction. A similar experiment using dansylated LHCII revealed that the protease degraded both WT and ore10 LHCII, indicating that its stability in ore10 perhaps did not result from a defect in the LHCII polypeptides themselves. Although protease activity was not present in non-senesced WT leaves, it was induced during DIS. It also was possible to diminish the high level of protease present in the thylakoids through high-salt washing, suggesting that this enzyme is extrinsically bound to the outer surface of the stroma-exposed thylakoid regions.

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  7. [국내논문]   Protective Role of Exogenous Spermidine against Paraquat Toxicity in Radish Chloroplasts  

    Ahn, Sun-Mi (Division of Life Sciences, Kangwon National University ) , Jin, Chang-Duck (Division of Life Sciences, Kangwon National University)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 338 - 347 , 2004 , 1226-9239 ,

    초록

    When radish chloroplasts were pretreated with 1 mM spermidine (Spd) and then exposed to 30 M paraquat (PQ), they improved their tolerance to subsequent PQ-induced oxidative damages. That included the decreases in the contents of chlorophyll, protein, and ascorbate, as well as the increases in malondialdehyde (MDA) and H2O2 levels. Analysis of antioxidant enzymes showed that Spd pretreatment effectively prevented the PQ-induced decreases in the total activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX). In contrast, the normally enhanced activities of dehydroascorbate reductase (DHAR) and glutathione reductase (GR) in PQ-treated chloroplasts were reversed by Spd pretreatment. In a native gel assay, the Cu/ZnSOD isozyme, which disappeared under the PQ alone treatment, was significantly recovered when tissues were pretreated with Spd. The dominant APX4 isozyme activity, which was preferentially decreased in response to PQ alone treatment, was also strongly reactivated by earlier Spd exposure. Therefore, we suggest that Spd could play a substantial role in protecting the radish chloroplasts from PQ stress. Furthermore, the enhancement of the Cu/ZnSOD and APX4 isozymes by Spd pretreatment seems to be responsible for prevention of the PQ-induced decreases in the total activities of SOD and APX, thereby providing a tolerance to PQ toxicity.

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  8. [국내논문]   Flowering Patterns among Angiosperm Species in Korea: Diversity and Constraints   피인용횟수: 1

    Kang, Hye-Soon (Department of Biology, College of Natural Sciences, Sungshin Women's University ) , Jang, Jung-Woon (Department of Biology, College of Natural Sciences, Sungshin Women's University)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 348 - 355 , 2004 , 1226-9239 ,

    초록

    Because of the complexities of their flowering parameters, it is difficult to make generalizations about flowering phenology among temperate angiosperms. We examined patterns of flowering time and duration among 2867 Korean angiosperm species, and these patterns were associated with climatic factors. The effect of taxonomic membership was also tested. Overall data pooled over species from 165 families showed that 63.8% of these angiosperms flowered in summer, 19.5% in spring, 16.5% in autumn, and 0.2% in winter. Summer and autumn flowering periods were significantly longer than springtime events. Both mean monthly temperature and precipitation were strongly positively correlated with the number of species in flower, but not with flowering duration. The seven largest families differed in their flowering season and duration; both parameters were also segregated among genera within large families. In a two-way analysis, both flowering season and family membership exerted significant effects on flowering duration. These results demonstrate that the evolutionary influence on flowering time and duration can be observed at the community level when two factors are considered, i.e., the interaction of flowering parameters and the taxonomic composition of species within those communities.

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  9. [국내논문]   Effect of Polyploidy Induction on Biomass and Ginsenoside Accumulations in Adventitious Roots of Ginseng   피인용횟수: 2

    Kim, Yun-Soo (Korea Ginseng Institute, Chung-Ang University ) , Hahn, Eun-Joo (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University ) , Murthy, Hosakatte Niranjana (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Department of Botany, Karnatak University ) , Paek, Kee-Yoeup (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 356 - 360 , 2004 , 1226-9239 ,

    초록

    Adventitious roots of Panax ginseng C.A. Meyer (a natural tetraploid) were treated with 50 or 100 mg L-1 colchicine for 12, 24, 36, 48, or 60 h to induce polyploid (octoploid) roots. The largest number of octoploid roots was obtained with a 100 mg L-1 colchicine treatment over 60 h. To verify that ginsenoside was being accumulated in the developing tissues, the tetraploid (control) and octoploid roots were cultured for 40 d in Murashige and Skoog media that lacked NH4NO3 but was supplemented with 2 mg L-1 naphthaleneacetic acid and 50 g L-1 sucrose. Levels of fresh and dry biomass were greater in the octoploid roots. Although total ginsenoside and Rb-group ginsenoside contents were less in the octoploid roots than in the tetraploids, the former had a higher amount of Rg-group ginsenosides (especially Rg1). These results demonstrate the benefit that polyploid adventitious roots provide in enhancing the production of secondary metabolites in ginseng.

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  10. [국내논문]   Effect of Growth Regulators on Asiaticoside Production in Whole Plant Cultures of Centella asiatica (L.) Urban   피인용횟수: 6

    Kim, Ok-Tae (Department of Biology, Chonnam National University ) , Kim, Min-Young (Department of Biology, Chonnam National University ) , Ahn, Jun-Cheul (Department of Life Sciences, Seonam University ) , Seong, Nak-Sul (National Institute of Crop Science, RDA ) , Hwang, Baik (Department of Biology, Chonnam National University)
    Journal of plant biology = 식물학회지 v.47 no.4 ,pp. 361 - 365 , 2004 , 1226-9239 ,

    초록

    We investigated the effects of growth regulators on whole-plant cultures derived from nodes of Centella asiatica. A B5 liquid medium including 0.01 mg L-1 2,4-D resulted in decreased growth and asiaticoside production. Among the cytokinins tested (TDZ, BA, zeatin, and kinetin), TDZ was the best supplement for the promotion of asiaticoside biosynthesis. To directly estimate this effect, we measured asiaticoside content in the leaf, the main organ for synthesis. The addition of TDZ did not affect asiaticoside accumulation. Nevertheless, our results suggest that treatment with exogenous TDZ may enhance the production of asiaticoside in cultures simply through an increase in biomass.

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    Fig. 1 이미지

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