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The Korean journal of parasitology 11건

  1. [국내논문]   Effects of anti-allergic drugs on intestinal mastocytosis and worm expulsion of rats infected with Neodiplostomum seoulense  

    Shin, Eun-Hee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center ) , Kim, Tae-Heung (Department of Dermatology, College of Medicine, Gyeongsang National University ) , Hong, Sung-Jong (Department of Parasitology, Chung-Ang University College of Medicine ) , Park, Jae-Hwan (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center ) , Guk, Sang-Mee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center ) , Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 81 - 87 , 2003 , 0023-4001 ,

    초록

    The effects of anti-allergic drugs on intestinal mastocytosis and the expulsion of Neodiplostomum seoulense were observed in Sprague-Dawley rats, after oral infection with 500 metacercariae. The drugs used were hydroxyzine (a histamine receptor H $_1$ blocker), cimetidine (a H $_2$ blocker), cyclosporin-A (a helper T-cell suppressant), and prednisolone (a T- and B-cell suppressant). Infected, but untreated controls, and uninfected controls, were prepared. Worm recovery rate and intestinal mastocytosis were measured on weeks 1, 2, 3, 5, and 7 post-infection. Compared with the infected controls, worm expulsion was significantly (P

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  2. [국내논문]   Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients  

    Ahn, Hye-Jin (Department of Parasitology and Catholic Institute of Parasitic Diseases, Catholic University of Korea, College of Medicine ) , Kim, Sera (Department of Parasitology and Catholic Institute of Parasitic Diseases, Catholic University of Korea, College of Medicine ) , Nam, Ho-Woo (Department of Parasitology and Catholic Institute of Parasitic Diseases, Catholic University of Korea, College of Medicine)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 89 - 96 , 2003 , 0023-4001 ,

    초록

    Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy The protein was not released into the parasitophorous vacuole during or after invasion. The cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg621. The full length cDNA sequence was completed with 5'-RACE as 1,592 bp, which contained open reading frame of 942 bp. The deduced amino acid sequence of Tg621 consisted of a polypeptide of 313 amino acids, with significant homology to ribosomal P proteins (RPP) of other organisms especially high to those of apicomplexan species. The expressed and purified TgRPP was assayed in western blot with the sera of toxoplasmosis patients and normal sera, which resulted in the 74.0% of positive reactions in toxoplasmosis patients whereas 8.3% in normal group. Therefore, the antibody formation against TgRPP in toxoplasmosis patients was regarded as specific for T. gondii infection and suggested a potential autoantibody.

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  3. [국내논문]   A case of acute gastric anisakiasis provoking severe clinical problems by multiple infection   피인용횟수: 2

    Noh, Ji-Hun (Department of Institute of Medical Science, Kosin Medical College ) , Kim, Bong-Jin (Department of Parastology, Kosin Medical College ) , Kim, Sun-Mee (Department of Parastology, Kosin Medical College ) , Ock, Mee-Sun (Department of Parastology, Kosin Medical College ) , Park, Moo-In (Department of Institute of Medical Science, Kosin Medical College ) , Goo, Ja-Young (Department of Institute of Medical Science, Kosin Medical College)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 97 - 100 , 2003 , 0023-4001 ,

    초록

    Acute gastric anisakiasis with multiple anisakid larvae infection is reported. A 68-year-old woman residing in Busan, Korea, had epigastric pain with severe vomiting about 5 hours after eating raw anchovies. Four nematode larvae penetrating the gastric mucosae in the great curvature of the middle body and fundus were found and removed during gasteroendoscopic examination. Another one thread-like moving larva was found in the great curvature of upper body on the following day. On the basis of their morphology, the worms were identified as the 3rd stage larvae of Anisakis simplex. This case is acute gastric anisakiasis provoking severe clinical problems by the multiple infection and the greatest number of anisakid larvae found in a patient in Korea.

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  4. [국내논문]   Identification of Acanthotrema felis (Digenea: Heterophyidae) metacercariae encysted in the brackish water fish Acanthogobius flavimanus   피인용횟수: 3

    Sohn, Woon-Mok (Department of Parasitology and Institute of Health Sciences, Gyeong National University College of Medicine ) , Han, Eun-Taek (Department of Parasitology, Kangwon National University College of Medicine ) , Seo, Min (Department of Parasitology, College of Medicine, Dankook University ) , Chai, Jong-Yil (Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 101 - 105 , 2003 , 0023-4001 ,

    초록

    The metacercariae of Acanthotrema felis Sohn et at., 2003 (Digenea: Heterophyidae) were discovered in a species of the brackish water fish, Acanthogobius flavimanus, in the Republic of Korea. They were experimentally fed to kittens, and adult flukes were harvested 7 days later. The adults were morphologically characterized by the presence of a bipartite seminal vesicle, the ventral sucker associated with a ventrogenital sac enclosing 3 sclerites (2 long and pointed, and 1 short and thumb-like), and an unarmed gonotyl. The adult flukes were identified as A. felis Sohn et al., 2003, and the brackish water fish A. flavimanus has been verified as one of its second intermediate hosts.

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  5. [국내논문]   Surface ultrastructure of the adult stage of Acanthotrema felis (Trematoda: Heterophyidae)  

    Sohn, Woon-Mok (Department of Parasitology and Institute of Health Sciences, Gyeongsang National University College of Medicine ) , Seo, Min (Department of Parasitology, College of Medicine, Dankook University ) , Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 107 - 111 , 2003 , 0023-4001 ,

    초록

    The surface ultrastructure of Acanthotrema felis (Trematoda: Heterophyidae) adults, recovered from a kitten experimentally infected with the metacercariae, was observed using a scanning electron microscope. The worm was leaf-like, ventrally concave and covered with scale-like multi-pointed tegumental spines. The spines on the anterior surface were short but broad, and had 10-12 pointed tips. The cytoplasmic processes protruded around the spines, like pockets for the spines. The ventrogenital opening was crescent, or kidney-shaped, and had protuberances with minute spines on its surrounding tegument. The spines on the posterior surface were long, but narrow, with 6-8 pointed tips. The cytoplasmic processes on this tegument were ridge-like, and elevated along the row of the spines. The surface ultrastructure of A. felis is generally similar to that of other heterophyid flukes, but some features are characteristic, and may be of taxonomic and bio-ecological significance.

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  6. [국내논문]   The intestinal parasite infection status of inhabitants in the Roxas city, the Philippines   피인용횟수: 1

    Kim, Bong-Jin (Department of Parasitology, Kosin Medical College ) , Ock, Mee-Sun (Department of Parasitology, Kosin Medical College ) , Chung, Dong-Il (Department of Parasitology, Kyungpook National University School of Medicine ) , Yong, Tai-Soon (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine ) , Lee, Kyu-Jae (Department of Parasitology and IFBB, Yonsei University Wonju College of Medicine)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 113 - 115 , 2003 , 0023-4001 ,

    초록

    We carried out a small-scale survey to investigate the status of intestinal protozoa and helminthes infection of inhabitants in Roxas city, Mindoro, the Philippines. Total 301 stool samples were subjected to the formalin-ether concentration method for the detection of helminth ova and protozoan cysts. The overall positive rate was 64.5%, and that of male and female were 56.6% and 72.5%, respectively. The highest infected helminth was Ascaris lumbricoudes (51.2%), followed by Trichuris trichiura (27.6%), hookworm (8.0%) and Enterobius vermicularis (0.3%). The protozoa infection status revealed that Entamoeba coli was the most frequent (15.0%). lodoamoeba buetschlii and E. histolytica were found but few. The multiple infection more than two parasites was 29.6%, and double infection with A. lumbricoides and T. trichiura was most common. The intestinal helminth infections were highly prevalent in this area, according to this result, and we concluded that anthelminthic drugs should be given to inhabiiants, especially to children of 1 to 15 years-old.

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  7. [국내논문]   A human case of Echinostoma hortense (Trematoda: Echinostomatidae) infection diagnosed by gastroduodenal endoscopy in Korea   피인용횟수: 4

    Cho, Chang-Min (Department of Internal Medicine, Kyungpook National University School Medicine ) , Tak, Won-Young (Department of Internal Medicine, Kyungpook National University School Medicine ) , Kweon, Young-Oh (Department of Internal Medicine, Kyungpook National University School Medicine ) , Kim, Sung-Kook (Department of Internal Medicine, Kyungpook National University School Medicine ) , Choi, Yong-Hwan (Department of Internal Medicine, Kyungpook National University School Medicine ) , Kong, Hyun-Hee (Department of Parasitology, Kyungpook National University School of Medicine ) , Chung, Dong-Il (Department of Parasitology, Kyungpook National University School of Medicine)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 117 - 120 , 2003 , 0023-4001 ,

    초록

    A human Echinostoma hortense infection was diagnosed by gastroduodenoscopy. An 81-year-old Korean male, living in Yeongcheon-shi, Gyeongsangbuk-do and with epigastric discomfort of several days duration, was subjected to upper gastrointestinal endoscopy. He was in the habit of eating fresh water fish. Two live worms were found in the duodenal bulb area and were removed using an endoscopic forcep. Based on their morphological characteristics, the worms were identified as E. hortense. The patient was treated with praziquantel 10 mg/kg as a single dose. The source of the infection in this case remains unclear, but the fresh water fish consumed, including the loach, may have been the source. This is the second case of E. hortense infection diagnosed by endoscopy in Korea.

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  8. [국내논문]   Usefulness of 8 kDa protein of Fasciola hepatica in diagnosis of fascioliasis  

    Kim, Kwang-Sig (Department of General Surgery, Cheju National University Hospital ) , Yang, Hyun-Jong (Department of Parasitology, College of Medicine, Ewha Womans University ) , Chung, Young-Bae (Department of Parasitology and Institute of Medical Science, College of Medicine, Cheju National University)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 121 - 123 , 2003 , 0023-4001 ,

    초록

    This study was designed to detect and evaluate an antigenicity of low molecular weight proteins of Fasciola hepatica in fascioliasis. Low molecular weight protein of F. hepatica was purified by ammonium sulfate precipitation and Sephacryl S-100 HR gel filtration. The protein obtained was estimated to be 8 kDa on 7.5-15% gradient sodium dodecyl sulfate gel electrophoresis. Immunoblotting studies showed that the 8 kDa protein reacted with human fascioliasis sera, but not other trematodiasis sera. This result suggests that the 8 kDa protein of F. hepatica is one of diagnostic antigens in human fascioliasis without cross-reaction with other human trematodiasis.

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  9. [국내논문]   Molecular identification of Korean Trichinella isolates   피인용횟수: 4

    Sohn, Woon-Mok (Department of Parasitology and Institute of Institute of Health Sciences, Gyeongsang National University College of Medicine ) , Hun, Sun (Department of Parasitology, College of Medicine, Hallym University ) , Chung, Dong-Il (Department of Parasitology, Kyungpook National University School of Medicine ) , Pozio, Edoardo (Laboratory of Parasitology, Instituto Superiore di Sanita)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 125 - 127 , 2003 , 0023-4001 ,

    초록

    Muscle larvae of Trichinella isolates from two outbreaks in Korea were analyzed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiple-PCR. All of the muscle larvae showed a band similar to that of T. spiralis larvae of the reference strain. The two Korean Trichinella isolates (isolate code ISS623 and ISS1078) might be classifiable to Trichinella spiralis.

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  10. [국내논문]   Cloning of a pore-forming subunit of ATP-sensitive potassium channel from Clonorchis sinensis  

    Hwang, Seung-Young (Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Busan 614-735, Korea. ) , Han, Hye-Jin (Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Busan 614-735, Korea. ) , Kim, So-Hee (Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Busan 614-735, Korea. ) , Park, Sae-Gwang (Department of Microbiology, Inje University College of Medicine, Busan 614-735, Korea. ) , Seog, Dae-Hyun (Department of Microbiology, Inje University College of Medicine, Busan 614-735, Korea. ) , Kim, Nari (Department of Physiology and Biophysics, Inje University College of Medicine, Busan 614-735, Korea. ) , Han, Jin (Department of Physiology and Biophysics, Inje University College of Medicine, Busan 614-735, Korea. ) , Chung, Joon-Yong (Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Busan 614-735, Korea. ) , Kho, Weon-Gyu (Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Busan 614-735, Korea.)
    The Korean journal of parasitology v.41 no.2 = no.117 ,pp. 129 , 2003 , 0023-4001 ,

    초록

    Muscle larvae of Trichinella isolates from two outbreaks in Korea were analyzed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiple-PCR. All of the muscle larvae showed a band similar to that of T. spiralis larvae of the reference strain. The two Korean Trichinella isolates (isolate code ISS623 and ISS1078) might be classifiable to Trichinella spiralis.

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