본문 바로가기
HOME> 저널/프로시딩 > 저널/프로시딩 검색상세

저널/프로시딩 상세정보

권호별목차 / 소장처보기

H : 소장처정보

T : 목차정보

Journal of microbiology and biotechnology 35건

  1. [국내논문]   Optimal Criterion for the Scale-Up Production of Schizophyllan in the Stirred Tank Reactor   피인용횟수: 1

    KWAK, JUNG-KI (Institute for Biotechnology, Technical University of Berlin ) , KOO, JAE-GUEN (College of Ocean Science and Technology, Kunsan University ) , PARK, SUNG-WOO (College of Ocean Science and Technology, Kunsan University ) , CHO, MAN-GI (Engineering Research Center, Dongseo University ) , KANG, BYEONG-CHUL (Engineering Research Center, Dongseo University ) , RAINER BUCHHOLZ (Institute for Biotechnology, Technical University of Berlin ) , PETER GOETZ (Institute for Biotechnology, Technical University of Berlin)
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 1 - 6 , 2005 , 1017-7825 ,

    초록

    Optimal criterion for the scale-up production of schizophyllan, a fungal polysaccharide secreted by Schizophyllum commune, was investigated. For the production of the polysaccharide in a 150-l bioreactor, the culture conditions optimized in a 15-l bioreactor were applied to a 150-l bioreactor with scale-up process, by changing impeller speed and airflow rate. The optimized impeller speed in the 15-l bioreactor was 50 rpm in a technical medium based on barley. For establishment of the scale-up process, 3 kinds of criteria were used while the gas throughput number was kept constant, as follows; constant volume-related power input, constant tip speed of stirrer, and constant Reynolds number. In the 150-l bioreactor, the highest values for the maximum specific growth rate (1.17/day) and productivity (0.63 g/L ${\cdot}$ day) were achieved in the culture condition from constant volumerelated power input criterion.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  2. [국내논문]   Optimization of Chitosan-Alginate Encapsulation Process Using Pig Hepatocytes or Development of Bioartificial Liver   피인용횟수: 4

    LEE , JI-HYUN (Samsung Biomedical Research Institute ) , LEE, DOO-HOON (Department of Chemical Engineering, Dongguk University ) , SON, JEONG-HWA (Department of Biotechnology and Bioengineering, Pukyong National University ) , PARK, JUNG-KEUG (Department of Chemical Engineering, Dongguk University ) , KIM, SUNG-KOO (Department of Biotechnology and Bioengineering, Pukyong National University)
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 7 - 13 , 2005 , 1017-7825 ,

    초록

    Chitosan-alginate capsules were formed by electrostatic interactions and exhibited an appropriate mechanical strength, permeability, and stability for the culture of hepatocytes. Pig hepatocytes were isolated and hepatocyte spheroids formed and immobilized in chitosan-alginate capsules. An encapsulation procedure of 3 min and spheroid formation period of 24 h were the optimum conditions for the best liver functions. Pig hepatocytes with a cell density of $6.0{\tomes}10^6$ cells/ml in the capsules were found to be most suitable for application in a bioartificial liver support system. The encapsulated pig hepatocyte spheroids exhibited stable ammonia removal and urea secretion rates in a bioreactor for 2 weeks. Accordingly, chitosan-alginate encapsulated hepatocyte spheroids in a packed-bed bioreactor would appear to have potential as a bioartificial liver.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  3. [국내논문]   New nirS-Harboring Denitrifying Bacteria Isolated from Activated Sludge and Their Denitrifying Functions in Various Cultures  

    LEE, SOO-YOUN (School of Life Sciences and Biotechnology, Korea University ) , LEE, SANG-HYON (School of Life Sciences and Biotechnology, Korea University ) , PARK, YONG-KEUN (School of Life Sciences and Biotechnology, Korea University)
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 14 - 21 , 2005 , 1017-7825 ,

    초록

    By using PCR with nirS gene primers, three nirSharboring denitrifying bacteria (strain N6, strain N23, and strain R13) were newly isolated from activated sludge of a weak municipal wastewater treatment plant. Small-subunit rRNA gene-based analysis indicated that strain N6, strain N23, and strain R13 were closely related to Arthrobacter sp.,Staphylococcus sp., and Bacillus sp., respectively. In an attempt to identify their roles in biological nitrate and nitrite removal from sewage, we investigated their specific denitrification rates (SDNRs) for $NO_-^3$ - and $NO_-^2$ - in various cultures. All purecultures of each isolated nirS-harboring bacterial strain could remove $NO_-^3$ - and $NO_-^2$ - simultaneously in high efficiency, and the carbon requirements for $NO_-^3$ - removal of strain N6 and strain R13 were effectively low at 3.1 and 4.1 g COD/g $NO_3N$ , respectively. In the case of mix-cultures of the strains (N6+N23, N6+R13, N23+R13, and N6+N23+R13), their SDNRs for $NO_-^3$ - were also effective, and their carbon requirements for $NO_-^3$ - removal were also effective at 3.0- 3.8 g COD/g NO3N. However, all tested mix-cultures accumulated $NO_-^2$ - in their culture media. On the other hand, the continuous culture of activated sludge mixed with strain N6 showed no significant increase of $NO_-^3$ - removal in comparison with strain N6's pure culture. These results suggest that nitrate and nitrite removal in biological wastewater treatment might be dependent on complicated bacterial interactions, including several effective denitrifying bacteria isolated in this study, rather than the specific bacterial types present and the number of bacterial types in activated sludge.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  4. [국내논문]   Isolation of Gibberellins-Producing Fungi from the Root of Several Sesamum indicum Plants   피인용횟수: 8

    CHOI, WHA-YOUL (Department of Microbiology, Kyungpook National University ) , RIM, SOON-OK (Department of Microbiology, Kyungpook National University ) , LEE, JIN-HYUNG (Department of Microbiology, Kyungpook National University ) , LEE, JIN-MAN (Department of Microbiology, Kyungpook National University ) , LEE, IN-JUNG (Department of Agriculture, Kyungpook National University ) , CHO, KANG-JIN (National Institute of Agricultural Biotechnology ) , RHEE, IN-KOO (Department of Agricultural Chemistry, Kyungpook National University ) , KWON, JUNG-BAE (Institute for Bioresources Research Gyeongbuk Provincial A.T.A. ) , KIM, JONG-GUK (Department of Microbiology, Kyungpook National University)
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 22 - 28 , 2005 , 1017-7825 ,

    초록

    Gibberellins (GAs) play important roles in plant growth and development. Fifty-four fungi were isolated from the roots of 4 kinds of Sesamum indicum plants, and the production of GAs was spectrophotometrically examined. The number of GA-producing fungi was two strains from S. indicum, four strains from Gold S. indicum, and five strains from Brown S. indicum. Eleven fungi with GAs-producing activity were incubated for seven days in 40 ml of Czapek's liquid medium at $25^{\circ}C$ and 120 rpm, and the amount of each GA in the medium was measured by gas chromatographymass spectrometery (GC-MS). Penicillium commune KNU5379 produced more $GA_3$ , $GA_4$ , and $GA_7$ than Gibberella fujikuroi, Fusarium proliferatum, and Neurospora crassa which are known as GAs-producing fungi. GAs-producing activity of the P. commune KNU5379 was shown to produce 71.69 ng of $GA_1$ , 252.42 ng of $GA_3$ , 612.00 ng of $GA_4$ , 259.00 ng of $GA_7$ , and 202.69 ng of $GA_9$ in 25 ml of liquid medium. Bioassay of culture fluid of GA-producing fungi was also performed on rice sprout.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  5. [국내논문]   Development of W/O/W Multiple Emulsion Formulation Containing Burkholderia gladioli   피인용횟수: 1

    KIM, HWA-JIN (Department of Biotechnology, Yonsei University ) , CHO, YOUNG-HEE (Department of Biotechnology, Yonsei University ) , BAE, EUN-KYUNG (Department of Biotechnology, Yonsei University ) , SHIN, TAEK-SU (Green Biotech Co., Ltd ) , CHOI, SUNG-WON (Green Biotech Co., Ltd ) , CHOI, KEE-HYUN (Green Biotech Co., Ltd ) , PARK, JI-YONG (Department of Biotechnology, Yonsei University, Department of Biotechnology, Yonsei University)
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 29 - 34 , 2005 , 1017-7825 ,

    초록

    W/O/W (water-in-oil-in-water) type multiple emulsion was applied to improve the storage stability of an antagonistic microorganism, Burkholderia gladioli. Encapsulation of microorganism into a W/O/W emulsion was conducted by using a two-step emulsification method. W/O/W emulsion was prepared by the incorporation of B. gladioli into rapeseed oil and the addition of polyglycerin polyriconolate (PGPR) and castor oil polyoxyethylene (COG 25) as the primary and secondary emulsifier, respectively. Microcrystalline cellulose was used as an emulsion stabilizer. To evaluate the usefulness of W/O/W emulsion formulation as a microbial pesticide for controlling the bacterial wilt pathogen (Ralstonia solanacearum), the storage stability and antagonistic activity of emulsion formulation were tested in vitro. The storage stability test revealed that the viability of formulated cells in emulsion was higher than that of unformulated cells in culture broth. At $4^{\circ}C$ , the viabilities of formulated cells and unformulated cells at the end of 20 weeks decreased to about 2 and 5 log cycles, respectively. At $37^{\circ}C$ , the viability of formulated cells decreased to only 2 log cycles at the end of storage. On the other hand, the viable cells in culture broth were not detected after 13 weeks. In activity test, formulated cells in emulsion were more effective in inhibiting the growth of pathogen than unformulated cells in culture broth. Unformulated cells completely lost their antagonistic activity during storage under similar conditions. The W/O/W multiple emulsion formulation was shown to be useful as the novel liquid formulation for biological control.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  6. [국내논문]   Identification of Recombinant Subtilisins   피인용횟수: 8

    CHOI , NACK-SHICK (Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, Department of Microbiology, Chungnam National University ) , YOO, KI-HYUN (Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) , YOON, KAB-SEOG (Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) , CHANG, KYU-TAE (Primate Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) , MAENG, PIL-JAE (Department of Microbiology, Chungnam National University ) , KIM, SEUNG-HO (Proteome Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 35 - 39 , 2005 , 1017-7825 ,

    초록

    To identify the activity of recombinant subtilisins (subtilisin BPN' and subtilisin Carlsberg), three different zymography methods, SDS-fibrin zymography (SDS-FZ), reverse fibrin zymography (RFZ), and isoelectric focusingfibrin zymography (IEF-FZ), were used. The recombinant subtilisins BPN' and Carlsberg did not migrate into the electrophoretic field based on a Laemmli buffer system, instead forming a "binding mode" at the top part of the separating gels with the SDS-FZ and RFZ techniques. Yet, this problem was resolved when using IEF-FZ with a pH range from 3 to 10. In addition, all these methods enabled the activity of a recombinant pro-subtilisin DJ-4 to be detected without a refolding pathway.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  7. [국내논문]   Modeling and Simulation of Simultaneous Saccharification and Fermentation of Paper Mill Sludge to Lactic Acid   피인용횟수: 3

    LIN, JIAN-QIANG (State Key Lab of Microbial Technology, School of Life Science, Shandong University ) , LEE, SANG-MOK (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University ) , KOO, YOON-MO (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University)
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 40 - 47 , 2005 , 1017-7825 ,

    초록

    Modeling and simulation for simultaneous saccharification and fermentation (SSF) process in bioconversion of paper mill sludge to lactic acid was carried out. The SSF process combined the enzymatic hydrolysis of paper mill sludge into glucose and the fermentation of glucose into lactic acid in one reactor. A mathematical modeling for cellulose hydrolysis was developed, based on the proposed mechanism of cellulase adsorption deactivation. Another model for simple lactic acid fermentation was also made. A whole mathematical model for SSF was developed by combining the above two models for cellulose hydrolysis and lactic acid fermentation. The characteristics of the SSF process were investigated using the mathematical model.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  8. [국내논문]   Comparison of Photorhabdus luminescens and Vibrio fischeri lux Fusions to Study Gene Expression Patterns   피인용횟수: 6

    MITCHELL, ROBERT J. (National Research Laboratory on Environmental Biotechnology, Depart. Environmental Science and Engineering, Institute of Science and Technology (GIST) ) , AHN, JOO-MYUNG (National Research Laboratory on Environmental Biotechnology, Depart. Environmental Science and Engineering, Institute of Science and Technology (GIST) ) , GU, MAN BOCK (National Research Laboratory on Environmental Biotechnology, Depart. Environmental Science and Engineering, Institute of Science and Technology (GIST))
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 48 - 54 , 2005 , 1017-7825 ,

    초록

    A comparison of promoter fusions with the luxCDABE genes from Vibrio fischeri and Photorhabdus luminescens was made using promoters from several genes (katG, sodA, and pqi-5) of E. coli that are responsive to oxidative damage. The respective characteristics, such as the basal and maximum bioluminescence and the relative bioluminescence, were compared. E. coli strains carrying fusions of the promoters to P. luminescens lux showed higher basal and maximally induced bioluminescent levels than strains carrying the same promoter fused to the luxCDABE genes from V. fischeri. The sensitivities between the strains were similar, regardless of the luciferase used, but lower response ratios were seen from strains harboring the P. luminescens lux fusions. Furthermore, using the two katG::lux fusion strains, the bioluminescence from the P. luminescens lux fusion strain, DK1, was stable after reaching a maximum, while that of strain DPD2511 decreased very rapidly due to substrate limitation.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  9. [국내논문]   Sophorolipid Production by Candida bombicola ATCC 22214 from a Corn-Oil Processing Byproduct   피인용횟수: 1

    KIM , HYUN-SOO (Nano Research Center, Moogunghwa Co. ) , KIM, YOUNG-BUM (Department of Biological Engineering, Inha University ) , LEE, BAEK-SEOK (Department of Biological Engineering, Inha University ) , KIM, EUN-KI (Department of Biological Engineering, Inha University)
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 55 - 58 , 2005 , 1017-7825 ,

    초록

    Sophorolipid was produced by Candida bombicola ATCC 22214 from soybean dark oil, a byproduct of soybean oil processing. With a fed-batch culture of C. bombicola for 7 days, 90 g/l of sophorolipid was obtained. The CMC (critical micelle concentration) and minimum surface tension of the sophorolipid in aqueous solution were found to be 150 mg/l and 48 mN/m, respectively. The dispersion capability of sophorolipid was higher than that of the chemical surfactants such as SDS and Brij30. The molar solubility ratio (MSR) of 4-methylnaphthalene was 0.2. Linoleic and oleic acids were the main constituents of the fatty acid composition of the sophorolipid. The sophorolipid showed antimicrobial activity against Propionibacterium acne and Bacillus subtilis.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  10. [국내논문]   Isolation and characterization of a new γ-polyglutamic acid producer Bacillus mesentericus MJM1, from Korean domestic Chungkukjang bean paste /  

    Zhao, Xin Qing , Park, Kwan Hyong , Jin, Ying Yu , Lee, In Hyung , Yang, Young Yell , Suh, Joo Won
    Journal of microbiology and biotechnology v.15 no.1 ,pp. 59 - 65 , 2005 , 1017-7825 ,

    초록

    Sophorolipid was produced by Candida bombicola ATCC 22214 from soybean dark oil, a byproduct of soybean oil processing. With a fed-batch culture of C. bombicola for 7 days, 90 g/l of sophorolipid was obtained. The CMC (critical micelle concentration) and minimum surface tension of the sophorolipid in aqueous solution were found to be 150 mg/l and 48 mN/m, respectively. The dispersion capability of sophorolipid was higher than that of the chemical surfactants such as SDS and Brij30. The molar solubility ratio (MSR) of 4-methylnaphthalene was 0.2. Linoleic and oleic acids were the main constituents of the fatty acid composition of the sophorolipid. The sophorolipid showed antimicrobial activity against Propionibacterium acne and Bacillus subtilis.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지

논문관련 이미지