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Journal of microbiology and biotechnology 35건

  1. [국내논문]   Identification of Aspergillus Strain with Antifungal Activity Against Phytophthora Species   피인용횟수: 2

    KANG SUNG WOO (Department of Chemical and Biological Engineering, Korea University ) , HONG SUK IN (Graduate School of Biotechnology, Korea University ) , KIM SEUNG WOOK (Applied Rheology Center (ARC), Korea University)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 227 - 233 , 2005 , 1017-7825 ,

    초록

    Fungal strain CGF was isolated from the soil of ChungNam Province, South Korea. Based on the 28S rDNA sequence analysis and the sequence of the internal transcribed spacer (ITS) region of ribosomal DNA, together with morphological and cultural characteristics, this strain was identified as Aspergillus sclerotiorum and renamed Aspergillus sclerotiorum CGF. This is the first strain of Aspergillus sclerotiorum identified in Korea. When the antifungal activity of A. sclerotiorum CGF was evaluated, among the phytopathogenic fungi, mycelial growth of only Phytophthora species was inhibited. Oermination of P. capsid zoospore was also inhibited. The bioactive compound of A. sclerotiorum CGF was highly thermo- and pH-stable.

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  2. [국내논문]   Immobilization of Glucose Oxidase on Multi-Wall Carbon Nanotubes for Biofuel Cell Applications   피인용횟수: 1

    JUNG SOO KEUN (School of Chemical Engineering and Bioengineering, College of Engineering, The University of Ulsan ) , CHAE YOUNG RAE (School of Chemical Engineering and Bioengineering, College of Engineering, The University of Ulsan ) , YOON JONG MOON (School of Chemical Engineering and Bioengineering, College of Engineering, The University of Ulsan ) , CHO BYUNG WON (Eco-Nano Research Center, Korea Institute of Science and Technology ) , RYU KEUN GARP (School of Chemical Engineering and Bioengineering, College of Engineering, The University of Ulsan)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 234 - 238 , 2005 , 1017-7825 ,

    초록

    Glucose oxidase was immobilized on the carboxylated multi-wall carbon nanotubes (MWNT-COOHs) in the presence of a coulping reagent, 1-ethy1-3-(3-dimethylaminopropy1) carbodiimide. Significant amounts of glucose oxidase were also immobilized on MWNT-COOHs without the coupling reagent. Various conditions for the immobilization of glucose oxidase were optimized. Optimal pH for the maximal activity of the immobilized glucose oxidase shifted to 7 from the optimal pH of 6 for the maximal activity of free enzyme due to the carboxy1 groups on the surface of MWNT-COOHs. An electrode of graphite rod with a diameter of 6 mm was fabricated using the immobilized glucose oxidase. The cyclic voltammetry study of the enzyme electrode revealed that the oxidation of glucose and subsequent transfer of electrons from the oxidation of glucose to the electrode were possible by the immobilized glucose oxidase without a mediator, implying that the enzyme electrode can be utilized for the development of biofuel cells.

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  3. [국내논문]   Characterization of Two Forms of Glucoamylase from Traditional Korean Nuruk Fungi, Aspergillus coreanus NR 15-1   피인용횟수: 3

    HAN YOUNG JIN (Department of Microbiology, College of Natural Science, Keimyung University ) , YU TAE SHICK (Department of Microbiology, College of Natural Science, Keimyung University)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 239 - 246 , 2005 , 1017-7825 ,

    초록

    Some characteristics of two forms of glucoamylase (glucan 1 A- $\alpha$ -glucosidase, EC 3. 2. I. 3) purified from Aspergillus coreanus NR 15-1 were investigated. The enzymes were produced on a solid, uncooked wheat bran medium of A. coreanus NR 15-1 isolated from traditional Korean Nuruk. Two forms of glucoamylase, GA-I and GA-II, were purified to homogenity after 5.8-fold and 9.6-fold purification, respectively, judged by disc- and SDS-polyacrylamide gel electrophoresis. The molecular mass of GA-I and GA-II were estimated to be 62 kDa and 90 kDa by Sephadex G-1OO gel filtration, and 64 kDa and 91 kDa by SDS-polyacrylarnide gel electrophoresis, respectively. The optimum temperatures of GA-I and GA-II were 60 $^circ$ C and 65 $^circ$ C, respectively, and the optimum pH was 4.0. The activation energy (Ea value) of GA-I and GA-II was 11.66 kcal/mol and 12.09 kcal/mol, respectively, and the apparent Michaelis constants (K_{m}) of GA-I and GA-II for soluble starch were found to be 3.57 mg/ml and 6.25 mg/ml, respectively. Both enzymes were activated by 1 mM Mn^{2+} and Cu^{2+}, but were completely inhibited by 1 mM N­bromosuccinimide. The GA-II was weakly inhibited by 1 mM p-CMB, dithiothreitol, EDTA, and pyridoxal 5-phosphate, but GA-I was not inhibited by those compounds. Both enzymes had significant ability to digest raw wheat starch and raw rice starch, and hydrolysis rates of raw wheat starch by GA-I and GA-II were 7.8- and 7.3-fold higher than with soluble starch, respectively.

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  4. [국내논문]   Comparison of Bacterial Cellulose Production in a Jar Fermentor Between Acetobacter xylinum BPR2001 and its Mutant, Acetan-Nonproducing Strain EP1   피인용횟수: 2

    BAE SANG OK (Chemical Resources Laboratory, Tokyo Institute of Technology ) , SUGANO YASUSHI (Chemical Resources Laboratory, Tokyo Institute of Technology ) , SHODA MAKOTO (Chemical Resources Laboratory, Tokyo Institute of Technology)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 247 - 253 , 2005 , 1017-7825 ,

    초록

    The bacterial cellulose (BC) production by a wild­strain Acetobacter xylinum BPR2001 and that by its acetan­nonproducing mutant, EPI, were compared in a jar fermentor. EPI produced about $28\%$ less BC than the wild-strain. The apparent difference in the cultivation of the two strains was the viscosity increase in the culture broth that was closely associated with acetan production. Increasing the viscosity of the culture broth of EPI by adding agar led to the formation of relatively small and uniform BC pellets, and BC production consequently became two-fold higher than that in the absence of agar and was almost equal to that by BPR2001. Therefore, acetan has an important role in BC production by inducing physical changes in the culture broth of the wild-type strain.

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  5. [국내논문]   In Vitro Formation of Protein Nanoparticle Using Recombinant Human Ferritin H and L Chains Produced from E. coli   피인용횟수: 7

    RO HYEON SU (BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology ) , PARK HYUN KYU (BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology ) , KIM MIN GON (BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology ) , CHUNG BONG HYUN (BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 254 - 258 , 2005 , 1017-7825 ,

    초록

    We have conducted in vitro reconstitution study of ferritin from its subunits FerH and FerL. For the reconstitution, FerH was produced from an expression vector construct in Escherichia coli and was purified from a heat treated cell extract by using one-step column chromatography. FerL was expressed as inclusion bodies. The denatured form of FerL was obtained by a simple washing step of the inclusion bodies with 3 M urea. The reconstitution experiment was conducted with various molar ratios of urea-denatured FerH and FerL to make the ferritin nanoparticle with a controlled composition of FerH and FerL. SDS-PAGE analysis of the reconstituted ferritins revealed that the reconstitution required the presence of more than 40 molar $\%$ of FerH in the reconstitution mixture. The assembly of the subunits into the ferritin nanoparticle was confmned by the presence of spherical particles with diameter of 10 nm by the atomic force microscopic image. Further analysis of the particles by using a transmission electron microscope revealed that the reconstituted particles exhibited different percentages of population with dense iron core. The reconstituted ferritin nanoparticles made with molar ratios of [FerH]/[FerL]=l00/0 and 60/40 showed that 80 to $90\%$ of the particles were apoferritin, devoid of iron core. On the contrary, all the particles formed with [FerH]/[FerL]=85/ 15 were found to contain the iron core. This suggests that although FerH can uptake iron, a minor portion of FerL, not exceeding $40\%$ at most, is required to deposit iron inside the particle.

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  6. [국내논문]   Use of Neonatal Chondrocytes for Cartilage Tissue Engineering   피인용횟수: 1

    KANG SUN WOONG (Department of Chemical Engineering, Hanyang University ) , PARK JUNG HO (Department of Orthopeadic Surgery, Ansan Hospital, College of Medicine, Korea University ) , KIM BYUNG SOO (Department of Chemical Engineering, Hanyang University)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 259 - 264 , 2005 , 1017-7825 ,

    초록

    Transplantation of cultured chondrocytes can regenerate cartilage tissues in cartilage defects in humans. However, this method requires a long culture period to expand chondrocytes to a large number of cells for transplantation. In addition, chondrocytes may dedifferentiate during long-term culture. These problems can potentially be overcome by the use of undifferentiated or partially developed cartilage precursor cells derived from neonatal cartilage, which, unlike chondrocytes from adult cartilage, have the capacity for rapid in vitro cell expansion and may retain their differentiated phenotype during long-term culture. The purpose of this study was to compare the cell growth rate and phenotypic modulation during in vitro culture between adult chondrocytes and neonatal chondrocytes, and to demonstrate the feasibility of regenerating cartilage tissues in vivo by transplantation of neonatal chondrocytes expanded in vitro and seeded onto polymer scaffolds. When cultured in vitro, chondrocytes isolated from neonatal (immediately postpartum, 2 h of age) rats exhibited much higher growth rate than chondrocytes isolated from adult rats. After 5 days of culture, more neonatal chondrocytes were in the differentiated state than adult chondrocytes. Cultured neonatal chondrocytes were seeded onto biodegradable polymer scaffolds and transplanted into athymic mice's subcutaneous sites. Four weeks after implantation, neonatal chondrocyte-seeded scaffolds formed white cartilaginous tissues. Histological analysis of the implants with hematoxylin and eosin showed mature and well-formed cartilage. Alcian blue/ safranin-O staining and Masson's trichrome staining indicated the presence of highly sulfated glycosarninoglycans and collagen, respectively, both of which are the major extracellular matrices of cartilage. Immunohistochemical analysis showed that the collagen was mainly type II, the major collagen type in cartilage. These results showed that neonatal chondrocytes have potential to be a cell source for cartilage tissue engineering.

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  7. [국내논문]   Effect of Bifidobacteria on Production of Allergy-Related Cytokines from Mouse Spleen Cells   피인용횟수: 12

    KIM HYE YOUNG (Department of Food and Nutrition, Seoul National University ) , YANG JIN OH (Research Center, Maeil Dairy Com ) , JI GEUN EOG (Department of Food and Nutrition, Seoul National University, Research Center, BIFIDO Co. Ltd, Research Institute of Human Ecology, Seoul National University)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 265 - 268 , 2005 , 1017-7825 ,

    초록

    To study the effect of bifidobacteria on preventing allergy response, levels of IFN- $\gamma$ , IgG2a, IL-4, and IgG1 were investigated in splenocytes isolated from ovalbumin (OVA)­sensitized allergic mice and BGN4-administered allergy­suppressed mice in the presence of various bifidobacterial strains. Most of the bifidobacteria, except 2A, increased production of Th I-associated immune markers, IFN - $\gamma$ and IgG2a. In addition, most of the bifidobacteria, except 2A and 19A, decreased production of IL-4, whereas the differences in the production of IgG1 were less pronounced. These results suggest that some strains of bifidobacteria may have the potential to prevent the occurrence of allergy by switching Th1/Th2-type antibodies and/or related cytokines.

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  8. [국내논문]   Screening and Biotransformation of Interleukin-1$\beta$ Converting Enzyme Production Inhibitors from Arctii fructus   피인용횟수: 4

    KIM HYUN A (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) , YOON DO YOUNG (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) , LEE SANG MYUNG (KT & G Central Research ) , BAEK SEUNG HWA (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) , HAN GYOON HEE (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) , KHO YOUNG HEE (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology ) , LEE CHOONG HWAN (Immune Modulator Research Laboratory, Korea Research Institute of Bioscience and Biotechnology)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 269 - 273 , 2005 , 1017-7825 ,

    초록

    Five dibenzylbutyrolactones were isolated from a methanol extract of Arctii fructus (Arctium lappa L.) by bioassay-guided isolation, using the interleukin-l $\beta$ converting enzyme (caspase-l, ICE) production inhibitory assay in vitro. These compounds were spectroscopically identified as lappaol E (1), lappaol A (2), matairesinol (3), arctigenin (4), and arctiin (5). Among the compounds tested, arctigenin (4) showed the strongest inhibitory activity for ICE production in IL- $\beta$ -induced proliferation of D 1 OS cells. Western blot analysis demonstrated that the arctigenin suppressed the expression of ICE protein in a dose-dependent manner. To estimate the biotransformation of Arctii fructus in vivo by human intestinal bacteria, we carried out an anaerobic incubation of the Arctii fructus extract with a human fecal suspension. From the HPLC analysis of metabolites, Arctiin (IC $_{50}$ =74.2<T 74.2 $\mu$ g/ml), a major component of Arctii fructus, was transformed to aglycone, arctigenin (IC $_{50}$ =12.5<T 12.5 $\mu$ g/ml), by human intestinal bacteria. The ICE production inhibitory activity of Arctii fructus would be much stronger in vivo than in vitro due to the biotransformation by human intestinal bacteria.

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  9. [국내논문]   Purification and Characterization of Chitinase from Paenibacillus illinoisensis KJA-424   피인용횟수: 4

    JUNG WOO JIN (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University ) , KUK JU HEE (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University ) , KIM KIL YONG (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University ) , KIM TAE HWAN (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University ) , PARK RO DONG (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 274 - 280 , 2005 , 1017-7825 ,

    초록

    A chitinase was purified from the culture supernatant of Paenibacillus illinoisensis KJA-424 by protein precipitation, DEAE-Sephadex anion-exchange chromatography, and Sephadex G-150 gel filtration. The molecular weight of the purified chitinase was 54 kDa on SDS-PAGE and activity staining. Optimal pH and temperature were pH 5.0 and 60 $^{circ}$ C, the presence of 10 ruM Ag $^{+}$ and Hg $^{2+}$ inhibited the activity by $92.1/%$ and $97.7/%$ , and the K $_{m}$ and V $_{max}$ values were 1.12 mg chitin mrl and 1.48 $\mu$ mol GlcNAc min $^{-1}$ , respectively. The enzyme hydrolyzed tetramer to dimer, pentamer to dimer and trimer, and hexamer to dimer, trimer and tetramer, indicating an endo-splitting mechanism. The chitinase had no hydrolytic activity toward dimer and trimer. The chitinase inhibited the mycelial growth of Rhizoctonia solani, suggesting an antifungal property.

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  10. [국내논문]   Electrochemical Regeneration of FAD by Catalytic Electrode Without Electron Mediator and Biochemical Reducing Power   피인용횟수: 4

    JEON SUNG JIN (Department of Biological Engineering, Seokyeong University ) , SHIN IN HO (Department of Biological Engineering, Seokyeong University ) , SANG BYUNG IN (Division of Water Environment and Remediation, KIST ) , PARK DOO HYUN (Department of Biological Engineering, Seokyeong University)
    Journal of microbiology and biotechnology v.15 no.2 ,pp. 281 - 286 , 2005 , 1017-7825 ,

    초록

    We created a new graphite-Cu(II) electrode and found that the electrode could catalyze FADH $_2$ oxidation and FAD reduction coupled to electricity production and consumption, respectively. In a fuel cell with graphite-Cu(II) anode and graphite-Fe(III) cathode, the electricity was produced by coupling to the spontaneous oxidation of FADH $_2$ Fumarate and xylose were not produced from the enzymatic oxidation of succinate and xylitol without FAD, respectively, but produced with FAD. The production of fumarate and xylose in the reactor with FAD electrochemically regenerated was maximally 2- 5 times higher than that in the reactor with FAD. By using this new electrode with catalytic function, a bioelectrocatalysts can be engineered; namely, oxidoreductase (e.g., lactate dehydrogenase) and FAD can function for biotransformation without an electron mediator and second oxidoreductase for cofactors recycling.

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    Fig. 1 이미지

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