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Journal of microbiology and biotechnology 35건

  1. [국내논문]   Assessment of Characteristics of Biofilm Formed on Autotrophic Denitrification   피인용횟수: 3

    JANG AM (Department of Civil and Environmental Engineering, University of Cincinnati ) , BUM MINSU (Department of Environmental Science and Engineering, Gwangju Institute of Science & Technology (GIST) ) , KIM SUNGYOUN (Department of Environmental Science and Engineering, Gwangju Institute of Science & Technology (GIST) ) , AHN YEONGHEE (Department of Environmental Science and Engineering, Gwangju Institute of Science & Technology (GIST) ) , KIM IN S (Department of Environmental Science and Engineering, Gwangju Institute of Science & Technology (GIST) ) , BISHOP PAUL L (Department of Civil and Environmental Engineering, University of Cincinnati)
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 455 - 460 , 2005 , 1017-7825 ,

    초록

    A pilot-scale sulfur particle autotrophic denitrification (SPAD) process for the treatment of municipal wastewater was operated for 10 months at Shihwa, Korea, and higher than $90\%\;NO^{-}_{3}-N$ removal efficiency was observed. Plate counting showed that the lower part of the denitrifying column reactor had the most autotrophic denitrifiers. The biofilm thickness formed on sulfur particles from the SPAD reactor was approximately $25-30\;{\mu}m$ , measured by DAPI (4,6-diamidino-2-phenylindole) staining. The presence of bacteria inside the highly porous sulfur particle was also monitored by SEM observation of the internal surfaces of broken sulfur particles. Biofilm extracellular polymeric substances (EPS) analysis showed that the ratio of carbohydrate to protein decreased with the reactor heights at which biofilm-formed sulfur particles were obtained.

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  2. [국내논문]   Biological Fixation of $CO_2$ by Chlorella sp. HA-1 in a Semi-Continuous and Series Reactor System   피인용횟수: 4

    LEE JAE-YOUNG (Transportation Key Technology Team, Korea Railroad Research Institute (KRRI) ) , KWON TAE-SOON (Environmental Remediation Engineering Laboratory, Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science & Technology (KAIST) ) , BAEK KITAE (Department of Environmental Engineering, Kumoh National Institute of Technology (KIT) ) , YANG JI-WON (Environmental Remediation Engineering Laboratory, Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science & Technology (KAIST))
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 461 - 465 , 2005 , 1017-7825 ,

    초록

    Characteristics of biological $CO_2$ fixation by Chlorella sp. HA-1 were investigated in a semi-continuous and series reactor system using an internally illuminated photobioreactor to overcome shortcomings of physicochemical technologies such as adsorption and membrane separation. High $CO_2$ fixation rate was achieved in the semi-continuous reactor system, in which the dilution ratios of the culture medium were controlled. The average $CO_2$ fixation rate was maintained almost constantly when the dilution ratio increased by 0.1 increment from the initial value of 0.5. The total removal efficiency of $CO_2$ was enhanced by employing a series reactor system. The average $CO_2$ fixation rate increased until 4.013 g $CO_2\;day^{-1}$ in a series operation of four reactors, compared to 0.986 g $CO_2\;day^{-1}$ in a batch operation mode. The total $CO_2$ fixation rate was proportional to the number of reactors used in the series reactor system. In the series reactor system of semi-continuous operation, a large amount of $CO_2$ was removed continuously for 30 days. These results showed that the present reactor systems are efficient and economically feasible for a biological $CO_2$ fixation.

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  3. [국내논문]   Overexpression and Characterization of appA Phytase Expressed by Recombinant Baculovirus-Infected Silkworm   피인용횟수: 4

    CHEN YIN (Sericultural Research Institute, Chinese Academy of Agricultural Sciences ) , ZHU ZHONGZE (Sericultural Research Institute, Chinese Academy of Agricultural Sciences ) , LIN XU'AI (Sericultural Research Institute, Chinese Academy of Agricultural Sciences ) , YI YONGZHU (Sericultural Research Institute, Chinese Academy of Agricultural Sciences ) , ZHANG ZHIFANG (Sericultural Research Institute, Chinese Academy of Agricultural Sciences ) , SHEN GUIFANG (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences)
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 466 - 471 , 2005 , 1017-7825 ,

    초록

    An Escherichia coli strain with high phytase activity was screened from pig excreta. The phytase gene, appA, was amplified by PCR technique. To obtain large amounts of appA phytase, the appA gene was subcloned into the baculovirus transfer vector pVL1393 under the control of the Polyhedrin promoter. The recombinant baculovirus harboring the appA gene was obtained after co-transfection and screening. The early $5^{th}$ instar larvae of silkworm were infected with the recombinant virus. Using this system, the appA phytase was overproduced up to 7,710 U per ml hemolymph. SDS-PAGE analysis revealed the baculovirus-derived appA phytase to be approximately 47 kDa in size. The optimal temperature and pH of the expressed phytase were $60^{\circ}C$ and pH 4.5, respectively. The enzymatic activity was increased by the presence of 1 mM $Ca^{2+}$ , 1 mM $Mn^{2+}$ , or $0.02\%$ Triton X-100.

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  4. [국내논문]   Preparation of Feather Digests as Fertilizer with Bacillus pumilis KHS-1   피인용횟수: 1

    Kim, Jin-Man (Department of Food Science and Biotechnology of Animal Resources, Konkuk University ) , Choi, Yang-Mun (Department of Food Service and Industry, Shinsung College ) , Suh, Hyung-Joo (Department of Food and Nutrition, College of Health Sciences, Korea University)
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 472 - 476 , 2005 , 1017-7825 ,

    초록

    The present study was untaken to assess the capacity of Bacillus pumilis KHS-1 to grow on chicken flour and to prepare feather digest as fertilizer. To increase keratinolytic activity, the addition of cysteine (5.0 mM) showed the highest keratinolytic activity (245 unit) among the reducing agents tested. The production of soluble protein (feather digests) paralleled the tendency to the production of keratinolytic protease. In the growth curve of B. pumilis KHS-1 at $30^{\circ}C$ in the feather medium with 5 mM cysteine, the maximum keratinolytic activity of B. pumilis was about 161 units/ml after 84 h of incubation. The maximum enzyme activities were observed at the late logarithmic growth phase, and remained thereafter with little changes. Using 27-day plant growth assays on carrot and Chinese cabbage, feather digests and reference fertilizer were compared. In terms of the length and the weight of the above-ground vegetations, feather digests showed the same effect as that of the fertilizer. Therefore, our investigation shows that the feather digests can be used in agriculture.

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  5. [국내논문]   Thymidine Production by Corynebacterium ammoniagenes Mutants   피인용횟수: 2

    Song, Kyung-Hwa (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies ) , Kwon, Do-Young (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies ) , Kim, Sang-Yong (BioNgene Co., Ltd. ) , Lee, Jung-Kul (BioNgene Co., Ltd. ) , Hyun, Hyung-Hwan (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 477 - 483 , 2005 , 1017-7825 ,

    초록

    Corynebacterium ammoniagenes ATCC 6872, which does not accumulate pyrimidine nucleoside or nucleotide, was metabolically engineered to secrete a large amount of thymidine. Characteristics of 5-fluorouracil resistance ( $FU^r$ ), hydroxyurea resistance ( $HU^r$ ), trimethoprim resistance ( $TM^r$ ), thymidylate phosphorylase deficiency ( $deoA^-$ ), inosine auxotrophy ( $ino^-$ ), 5-fluorocytosine resistance ( $FC^r$ ), thymidine kinase deficiency, and thymidine resistance ( $thym^r$ ) were successively introduced into mutant strains KR3 and DY5T9-5, and shake-flask cultures were able to accumulate 408.1 mg/l and 428.2 mg/l of thymidine, respectively, as a major product. The mutant strains did not accumulate thymine at all and accumulated less than 10 mg/l of other pyrimidine nucleosides, such as cytosine, cytidine, and deoxycytidine, as byproducts.

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  6. [국내논문]   DNA Repair Activity of Human rpS3 is Operative to Genotoxic Damage in Bacteria   피인용횟수: 3

    JANG CHANG-YOUNG (Laboratory of Biochemistry, School of Life Sciences & Biotechnology, and Bioinstitute, Korea University ) , LEE JAE YUNG (Department of Biology, Mokpo National University ) , KIM JOON (Laboratory of Biochemistry, School of Life Sciences & Biotechnology, and Bioinstitute, Korea University)
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 484 - 490 , 2005 , 1017-7825 ,

    초록

    Human ribosomal protein S3 (rpS3), which has a DNA repair endonuclease activity, is a multifunctional protein. This protein is involved in DNA repair, translation, and apoptosis. In particular, rpS3 has a lyase activity, which cleaves the phosphodiester bond of damaged sites such as cyclobutane pyrimidine dimers and AP sites. Here, using deletion analysis, we identified that the repair endonuclease domain resides in the C-terminal region (165-243 aa) of rpS3. We also found that ectopic expression of GST-rpS3 in bacterial strain BL21 promoted the resistance of these cells to ultraviolet (UV) radiation and hydrogen peroxide ( $H_{2}O_{2}$ ) treatment. The repair domain of rpS3 was sufficient to exhibit the resistance to UV irradiation and recover cell growth and viability, showing that the repair activity of rpS3 is responsible for the resistance to UV irradiation. Our study suggests that rpS3 is able to process DNA damage in bacteria via its repair domain, showing the resistance to genotoxic stress. This implies that rpS3-like activity could be operative in bacteria.

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  7. [국내논문]   Isolation and Characterization of Kasugamycin Biosynthetic Genes from Streptomyces kasugaensis KACC 20262   피인용횟수: 5

    JO YOU-YOUNG (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University ) , LIU JING (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University ) , JIN YING-YU (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University ) , YANG YOUNG-YELL (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University ) , SUH JOO-WON (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University)
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 491 - 496 , 2005 , 1017-7825 ,

    초록

    The biosynthetic gene cluster for the aminoglycoside antibiotic kasugamycin was isolated and characterized from the kasugamycin producing strain, Streptomyces kasugaensis KACC 20262. By screening a fosmid library using kasA, the gene encoding aminotransferase, we isolated a 22 kb DNA fragment. The fragment contained seventeen complete open reading frames (ORFs); one of these ORFs, kasD, was identified as the gene for dNDP-glucose 4,6-dehydratase, which catalyzes the conversion of dNDP-glucose to 4-keto-6-deoxy-dNDP-glucose. The enzyme showed a broad spectrum of substrate specificity. In addition, ksR was overexpressed in E. coli BL21 and proved to be a self-resistance gene against kasugamycin. These findings suggest that the isolated gene cluster is highly likely responsible for the biosynthesis of kasugamycin.

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  8. [국내논문]   Mercury Ion Removal Using a Packed-Bed Column with Granular Aminated Chitosan   피인용횟수: 6

    JEON, CHOONC (Department of Environmental & Applied Chemical Engineering, Kangnung National University)
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 497 - 501 , 2005 , 1017-7825 ,

    초록

    This study deals with the removal of mercury species using a packed-bed column with spherical aminated chitosan material. These adsorbents revealed a high adsorption capacity for mercury species. Experiments with feed solutions of 10 ppm Hg dissolved in distilled water showed an excellent removal with a sharp increase of the filter effluent concentration after a total throughput of 900 bed volumes of feed water. Up to $95\%$ desorption was reached by using 3 bed volumes of 0.01 N EDTA solution. EDTA could be recovered by means of sulfuric acid with about $75\%$ efficiency. Almost the same results were obtained in repeated sorption and desorption experiments at identical conditions. The experiments demonstrated that the sorbents possessed practically no sorption capacity for alkaline earth ions ( $Ca^{2+}\;and\;Mg^{2+}$ ). Their influence on the sorption of mercury was negligible. In experiments with spiked tap water of the Karlsruhe Research Centre and a feed mercury concentration of 0.01 mg/l, the breakthrough of Hg was observed only after a total throughput of about 6,000 bed volumes of feed water.

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  9. [국내논문]   A Genetic Marker Associated with the A1 Mating Type Locus in Phytophthora infestans   피인용횟수: 4

    KIM KWON-JONG (Division of Bio-resources Technology, College of Agriculture and Life Sciences, Kangwon National University ) , EOM SEUNG-HEE (Kangwon Agricultural Technology Administration ) , LEE SANG-PYO (Division of Bio-resources Technology, College of Agriculture and Life Sciences, Kangwon National University ) , JUNG HEE-SUN (Division of Bio-resources Technology, College of Agriculture and Life Sciences, Kangwon National University ) , KAMOUN SOPHIEN (Department of Plant Pathology, Ohio State University ) , LEE YOUN SU (Division of Bio-resources Technology, College of Agriculture and Life Sciences, Kangwon National University)
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 502 - 509 , 2005 , 1017-7825 ,

    초록

    Sexual reproduction plays an important role in the biology and epidemiology of oomycete plant pathogens such as the heterothallic species Phytophthora infestans. Recent worldwide dispersal of A2 mating type strains of P. infestans resulted in increased virulence, gene transfer, and genetic variation, creating new challenges for disease management. To develop a genetic assay for mating type identification in P. infestans, we used the Amplified Fragment Length Polymorphism (AFLP) technique. The primer combination E+AT/M+CTA detected a fragment specific to A1 mating type (Mat-A1) of P. infestans. This fragment was cloned and sequenced, and a pair of primers (INF-1, INF-2) were designed and used to differentiate P. infestans Mat-A1 from Mat-A2 strains. The Mat A1-specific fragment was detected using Southern blot analysis of PCR products amplified with primers INF-1 and INF-2 from genomic DNA of 14 P. infestans Mat-A1 strains, but not 13 P. infestans Mat-A2 strains or 8 other isolates representing several Phytophthora spp. Southern blot analysis of genomic DNAs of P. infestans isolates revealed a 1.6 kb restriction enzyme (EcoRI, BamHI, AvaI)-fragment only in Mat-A1 strains. The A1 mating type-specific primers amplified a unique band under stringent annealing temperatures of $63^{\circ}C-64^{\circ}C$ , suggesting that this PCR assay could be developed into a useful method for mating type determination of P. infestans in field material.

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  10. [국내논문]   PLGA Microspheres in Hyaluronic Acid Gel as a Potential Bulking Agent for Urologic and Dermatologic Injection Therapies   피인용횟수: 4

    KANG SUN-WOONG (Department of Chemical Engineering, Hanyang University ) , CHO EUI RI (Department of Chemical Engineering, Hanyang University ) , KIM BYUNG-SOO (Department of Chemical Engineering, Hanyang University)
    Journal of microbiology and biotechnology v.15 no.3 ,pp. 510 - 518 , 2005 , 1017-7825 ,

    초록

    In this study, we investigated whether PLGA microspheres in combination with hyaluronic acid (HA) gel have appropriate properties as a bulking agent for urologic injection therapies and whether the implantation of PLGA microspheres and HA gel induces angiogenesis in the newly formed tissues. In order to investigate whether this bulking agent is injectable, this material was injected through 24-gauge needles into the subcutaneous dorsum of the mouse. The bulking agent was easily injected without needle obstruction. Histological analyses of the hybrid tissues at 2 weeks showed that host cells at the surrounding tissues migrated into the spaces between the implanted PLGA microspheres and formed tissue-like structures. An inflammatory response to the implants was mild at 2 weeks and diminished at 8 weeks. Importantly, extensive ingrowth of blood vessels was observed in the hybrid tissues formed by the injection of PLGA microspheres and HA, whereas blood vessels rarely formed in the hybrid tissues formed by the injection of PLGA microspheres only. The implant volume was conserved for almost the entire implantation period. Histological analyses of the distant organs of the bulking agent-implanted animals, such as the lungs, liver, heart, brain, kidney, and spleen, showed no evidence of the injected microsphere migration. These results show that PLGA microspheres in combination with HA possess the appropriate characteristics for a bulking agent for urologic injection therapies and induce extensive blood vessel formation in the hybrid tissues.

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