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Fungal genetics and biology : FG & B 7건

  1. [해외논문]   Editorial Board and barcode  


    Fungal genetics and biology : FG & B v.108 ,pp. IFC , 2017 , 1087-1845 ,

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  2. [해외논문]   Editorial Board and barcode   SCI SCIE


    Fungal genetics and biology : FG & B v.108 ,pp. IFC - IFC , 2017 , 1087-1845 ,

    초록

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    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   The novel Aspergillus fumigatus MAT1-2-4 mating-type gene is required for mating and cleistothecia formation   SCI SCIE

    Yu, Yidong (Mikrobiologisches Institut –) , Amich, Jorge (Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen and Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany ) , Will, Cornelia (Research Center for Infectious Diseases, Julius-Maximilians-Universität Würzburg, Germany ) , Eagle, Carly E. (Mikrobiologisches Institut –) , Dyer, Paul S. (Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen and Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany ) , Krappmann, Sven (School of Life Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom )
    Fungal genetics and biology : FG & B v.108 ,pp. 1 - 12 , 2017 , 1087-1845 ,

    초록

    Abstract Sexual propagation accompanied by recombination and the formation of spore-containing fruiting bodies is a cornerstone of fungal genetics and biology. In the human pathogen Aspergillus fumigatus sexual identity has previously been shown to be determined by MAT1 - 1 - 1 or MAT1-2 - 1 genes which act as transcriptional regulators and are present within idiomorphs found at the MAT locus. We here report the identification and first characterization of a further novel gene, termed MAT1-2 - 4 , that is present in the MAT1-2 idiomorph of A. fumigatus . A mating-type swapping strategy was used to achieve an unbiased deletion of the MAT1-2 - 4 gene with no impact on MAT1-2 - 1 gene expression. Phenotypical characterization of the resulting strain revealed an inability to mate with the compatible MAT1-1 progenitor, demonstrating that the MAT1-2 - 4 gene product is a genuine mating-type factor required for correct sexual development. A GPI-anchored protein of unknown function was identified as interaction partner. However, no functional role in the mating process or ascosporogenesis could be demonstrated by deletion analysis for this latter protein, although a role in heterokaryon formation is suggested. Bioinformatic analysis also demonstrated the presence of MAT1-2 - 4 homologues in some, but not all, other Aspergillus species and the evolutionary origins and implications of the MAT1-2 - 4 gene are discussed. Highlights First characterization of a novel Aspergillus fumigatus mating-type gene: MAT1-2 - 4 . MAT1-2 - 4 homologues are present in some, but not all, other Aspergillus species. Deletion of the MAT1-2 - 4 gene was achieved by a mating-type swapping strategy. A MAT1-2-4Δ deletion strain does not mate with the compatible MAT1-1 progenitor. A GPI-anchored protein was identified as MAT1-2-4 interaction partner.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  4. [해외논문]   A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome   SCI SCIE

    Upadhya, Rajendra (Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA ) , Lam, Woei C. (Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA ) , Maybruck, Brian T. (Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA ) , Donlin, Maureen J. (Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St. Louis, MO, USA ) , Chang, Andrew L. (Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA ) , Kayode, Sarah (Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA ) , Ormerod, Kate L. (Australian Infectious Diseases Research Centre and School of Chemistry& Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia ) , Fraser, James A. (Australian Infectious Diseases Research Centre and School of Chemistry& Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia ) , Doering, Tamara L. (Department of Molecular Microbiology, Washingto) , Lodge, Jennifer K.
    Fungal genetics and biology : FG & B v.108 ,pp. 13 - 25 , 2017 , 1087-1845 ,

    초록

    Abstract C. neoformans is an encapsulated fungal pathogen with defined asexual and sexual life cycles. Due to the availability of genetic and molecular tools for its manipulation, it has become a model organism for studies of fungal pathogens, even though it lacks a reliable system for maintaining DNA fragments as extrachromosomal plasmids. To compensate for this deficiency, we identified a genomic gene-free intergenic region where heterologous DNA could be inserted by homologous recombination without adverse effects on the phenotype of the recipient strain. Since such a site in the C. neoformans genome at a different location has been named previously as “safe haven”, we named this locus second safe haven site ( SH2 ). Insertion of DNA into this site in the genome of the KN99 congenic strain pair caused minimal change in the growth of the engineered strain under a variety of in vitro and in vivo conditions. We exploited this ‘safe’ locus to create a genetically stable highly fluorescent strain expressing mCherry protein (KN99mCH); this strain closely resembled its wild-type parent (KN99α) in growth under a variety of in vitro stress conditions and in the expression of virulence traits. The efficiency of phagocytosis and the proliferation of KN99mCH inside human monocyte-derived macrophages were comparable to those of KN99α, and the engineered strain showed the expected organ dissemination after inoculation, although there was a slight reduction in virulence. The mCherry fluorescence allowed us to measure specific association of cryptococci with leukocytes in the lungs and mediastinal lymph nodes of infected animals and, for the first-time, to assess their live/dead status in vivo . These results highlight the utility of KN99mCH for elucidation of host-pathogen interactions in vivo . Finally, we generated drug-resistant KN99 strains of both mating types that are marked at the SH2 locus with a specific drug resistant gene cassette; these strains will facilitate the generation of mutant strains by mating. Highlights A second safe haven site ( SH2 ) in the C. neoformans genome that is amenable for targeted heterologous DNA insertions. A strain encoding mCherry at the SH2 site (KN99mCH) is highly fluorescent. KN99mCH phenotypes are comparable to those of the parent KN99α strain. KN99mCH is ideal for in vitro and in vivo host-pathogen interaction studies employing fluorescence detection. C. neoformans marked at the SH2 locus will facilitate studies involving genetic crosses.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   The two-component response regulator VdSkn7 plays key roles in microsclerotial development, stress resistance and virulence of Verticillium dahliae   SCI SCIE

    Tang, Chen (Corresponding author.) , Xiong, Dianguang , Fang, Yulin , Tian, Chengming , Wang, Yonglin
    Fungal genetics and biology : FG & B v.108 ,pp. 26 - 35 , 2017 , 1087-1845 ,

    초록

    Abstract The fungus Verticillium dahliae causes vascular wilt disease on various plant species resulting in devastating yield losses worldwide. The capacity of V. dahliae to colonize in host plant xylem and disseminate by microsclerotia has led to studies to evaluate genes associated with pathogenesis and microsclerotia formation. Here, we identified and characterized a V. dahliae homolog to Skn7, a two-component stress response regulator of Saccharomyces cerevisiae . Results showed that melanized microsclerotia formation and conidiation were significantly inhibited in the VdSkn7 deletion mutants. VdSkn7 -deficient mutants displayed severe growth defect under heat shock, cell wall perturbing agents and H 2 O 2 , and were significantly less virulent but were not sensitive to osmotic stresses compared to the wild-type strain. Finally, we demonstrated that VdSkn7 is required for the plant penetration. Taken together, our study thus provides new evidence on the functional conservation and divergence of Skn7 orthologs among fungal organisms and indicates that VdSkn7 contributes to microsclerotial development, virulence and stress response of V. dahliae . Highlights Disruption of VdSkn7 postpones microsclerotia formation. VdSkn7 regulates thermal stress response. VdSkn7 mutant exhibits high sensitivity to H 2 O 2 stress response. Deletion of VdSkn7 attenuates virulence and impairs infectious ability.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [해외논문]   The calcium-binding protein EpANN from the lichenized fungus Endocarpon pusillum enhances stress tolerance in yeast and plants   SCI SCIE

    Zhang, Yongli (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10010, PR China ) , Li, Hui (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10010, PR China ) , Wang, Yanyan (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10010, PR China ) , Wei, Jiangchun (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 10010, PR China)
    Fungal genetics and biology : FG & B v.108 ,pp. 36 - 43 , 2017 , 1087-1845 ,

    초록

    Abstract Annexins are calcium-phospholipid binding proteins that play a significant role in the Ca 2+ signaling pathway. These proteins are essential for plants to effectively respond to abiotic stresses. However, their functions and mechanisms remain largely unknown in fungi. In this study, an annexin gene, Epann , was cloned from the lichenized fungus Endocarpon pusillum , a drought resistant organism. Our results showed that Epann was induced by several abiotic stresses in E. pusillum . Heterologous expression of the Epann gene enhanced the stress tolerance of Saccharomyces cerevisiae . Under heat-shock conditions, the EpANN proteins were significantly aggregated and the aggregation sites were located on peroxisomes. In heat-shocked cells, Epann reduced the reactive oxygen species level mainly through its intracellular peroxidase activity and regulation of stress-related genes. Transgenic Arabidopsis plants overexpressing Epann exhibited a higher germination rate under oxidative stress and stronger drought tolerance. Our results provide a mechanistic understanding of the role of annexins in abiotic stress responses and suggest that this lichenized fungal gene could be a promising resource to generate stress-tolerant transgenic organisms. Highlights Epann -transgenic yeast had improved abiotic stress resistance, especially to heat. Under heat stress, EpANN-GFP protein accumulated in peroxisomes in transgenic yeast. Epann reduced ROS levels mainly through intracellular peroxidase activity. Transgenic Arabidopsis germinated better under stress and had drought tolerance.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [해외논문]   High-density genetic mapping identifies the genetic basis of a natural colony morphology mutant in the root rot pathogen Armillaria ostoyae   SCI SCIE

    Heinzelmann, Renate (Swiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland ) , Croll, Daniel (Laboratory of Evolutionary Genetics, Institute of Biology, University of Neuchâtel, Rue Emile-Argand 11, CH-2000 Neuchâtel, Switzerland ) , Zoller, Stefan (Genetic Diversity Centre, ETH Zürich, Universitätstrasse 16, CH-8092 Zürich, Switzerland ) , Sipos, Gyö (Functional Genomics and Bioinformatics Group, Research Center for Forestry and Wood Industry, University of Sopron, Bajcsy-Zsilinszky. u. 4, H-9400 Sopron, Hungary ) , rgy (Functional Genomics and Bioinformatics Group, Research Center for Forestry and Wood Industry, University of Sopron, Bajcsy-Zsilinszky. u. 4, H-9400 Sopron, Hungary ) , Mü (Department of Genome-oriented Bioinformatics, Center of Life and Food Science Weihenstephan, Technische Universität München, Maximus-von-Imhof-Forum 3, D-85354 Freising, Germany ) , nsterkö (Swiss Federal Research Institute WSL, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland) , tter, Martin , Gü , ldener, Ulrich , Rigling, Daniel
    Fungal genetics and biology : FG & B v.108 ,pp. 44 - 54 , 2017 , 1087-1845 ,

    초록

    Abstract Filamentous fungi exhibit a broad spectrum of heritable growth patterns and morphological variations reflecting the adaptation of the different species to distinct ecological niches. But also within species, isolates show considerable variation in growth rates and other morphological characteristics. The genetic basis of this intraspecific variation in mycelial growth and morphology is currently poorly understood. By chance, a growth mutant in the root rot pathogen Armillaria ostoyae was discovered. The mutant phenotype was characterized by extremely compact and slow growth, as well as shorter aerial hyphae and hyphal compartments in comparison to the wildtype phenotype. Genetic analysis revealed that the abnormal phenotype is caused by a recessive mutation, which segregates asa single locus in sexual crosses. In order to identify the genetic basis of the mutant phenotype, we performed a quantitative trait locus (QTL) analysis. A mapping population of 198 haploid progeny was genotyped at 11,700 genome-wide single nucleotide polymorphisms (SNPs) making use of double digest restriction site associated DNA sequencing (ddRADseq). In accordance with the genetic analysis, a single significant QTL was identified for the abnormal growth phenotype. The QTL confidence interval spans a narrow, gene dense region of 87kb in the A. ostoyae genome which contains 37 genes. Overall, our study reports the first high-density genetic map for an Armillaria species and shows its successful application in forward genetics by resolving the genetic basis of a mutant phenotype with a severe defect in hyphal growth. Highlights The first high-density and quality genetic map for Armillaria ostoyae is presented. A single QTL linked to defective hyphal growth is identified. The QTL confidence interval contains 37 genes and is characterized by a 100% phenotype-genotype association.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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