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The journal of microbiology 12건

  1. [국내논문]   Phylogenetic Study of Trichaptum Species Based on the RFLP Analysis of Mitochondrial DNA   피인용횟수: 2

    Kim, Mi-Sun (Research Center for Molecular Microbiology, Seoul National University ;) , Jung, Hack-Sung
    The journal of microbiology v.34 no.3 ,pp. 215 - 219 , 1996 , 1225-8873 ,

    초록

    Eight strains of Trichaptun (Polyporaceae), two strains from each species of T. abietinum, T. biforme, T. fusco-violaceum, and T. laricinum were examined to see their phylogenetic relationship by digesting mitochondrial DNAs with EcoRV, Hind III, XbaI, and PstI, and then analyzing fragmentation patterns with the methods of Nei and Li. T. abietinum, T. biforme, and T. laricinum developed an independent phylogenetic lineage, respectively, but T. fusco-violaceum FP-133997-sp showed a close relationship with two strains of T. bioforme, and T. fusco-violaceum HHB-4016-sp barely grouped with those of T. laricinum. Based on the results of the RFLP analysis of mtDNA, it is concluded that T. fusco-violaceum is under way to differentiation into two different subgroups.

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  2. [국내논문]   Ultrastructural Studies of Encystment in Allomyces macrogynus  

    Kim, Jung-Soeup (Department of Microbiology, INJE University ) , Youn, Hyun-Joo (Department of Microbiology, INJE University ) , Cho, Chung-Won (Department of Microbiology, INJE University)
    The journal of microbiology v.34 no.3 ,pp. 220 - 224 , 1996 , 1225-8873 ,

    초록

    Ultrastructural organization of encysting zoospores of Allomyces macrogynus was examined using the methods of cryofixation and freeze substitution. During enxcystment, obvious changes were observed at the surface of the plasma membrane and in the structure of gamma particles. Many multivesicular bodies associated with the plasma membrane were observed at early stages of encystment. After induction of encystment, vesicles were found within the gamma particles. These vesicles appeared to leave gamma particles after forming multivesicular bodies. This study suggests that the cell wall formation during encystment is mediated by the fusion of multivesicular bodies with the plasma membrane.

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  3. [국내논문]   Analysis of cellular fatty acid methyl esters (FAMEs) for the identification of leuconostoc strains isolated from kimchi  

    Lee, Jung-Sook (Korean Collection for Type Cultures, Korea Researh Institute of Borsciene and Biotechnlolgy, KIST ) , Chun, Chang-Ouk (Korean Collection for Type Cultures, Korea Researh Institute of Borsciene and Biotechnlolgy, KIST ) , Kim, Hong-Joong (Korean Collection for Type Cultures, Korea Researh Institute of Borsciene and Biotechnlolgy, KIST ) , Joo, Yun-Jung (Cellular Response Modifier Research Unit. Korea Research Institute of Biscience and Biotechnology. KIST ) , Lee, Hun-Joo (Cellular Response Modifier Research Unit. Korea Research Institute of Biscience and Biotechnology. KIST ) , Park, Chan-Sum (Cellular Response Modifier Research Unit. Korea Research Institute of Biscience and Biotechnology. KIST ) , Park, Yong-Ha (Korean Collection for Type Cultures, Korea Researh Institute of Borsciene and Biotechnlolgy, KIST ) , Mheen, Tae-Ick (Korean Collection for Type Cultures, Korea Researh Institute of Borsciene and Biotechnlolgy, KIST)
    The journal of microbiology v.34 no.3 ,pp. 225 - 228 , 1996 , 1225-8873 ,

    초록

    The cellular fatty acid methyl esters (FAMEs) analysis data obtained for clusters defined at a Euclidian distance of 17.5, in the classification of lactic acid bacteria isolated from kimchi, described by Lee et al. (4), was used for the identification of 79 Leuconostoc isolates. The test strains were isolated using a selective isolation medium specific for the genus Leuconostoc. These strains were then characterized according to their fatty acid profiles. The results show that all seventy nine test strains were identified to the known Leuconostoc clusters B, C, and D. Cluster B had the highest relative amount of the saturated fatty acid 16 : 0. The saturated fatty acid 16 : 0 and summed feature 9 were found as a major components in cluster C, which had a higher level of summed feature 9 than cluster B. Cluster D is characterized by the highest relative amount of the unsaturated fatty acid 18 : 1 w9c. It is suggested that FAMEs analysis can be successfully applied in the identification of lactic acid bacteria isolated from kimchi.

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  4. [국내논문]   Direct Extraction of DNA from Soil for Amplification of 16S rRNA Gene Sequences by Polymerase Chain Reaction   피인용횟수: 2

    Cho, Jae-Chang (Research Center for Molecular Microbiology, Seoul National University ) , Lee, Dong-Hun (Research Center for Molecular Microbiology, Seoul National University ) , Cheol, Cho-Young (Research Center for Molecular Microbiology, Seoul National University ) , Cho, Jang-Cheon (Research Center for Molecular Microbiology, Seoul National University ;) , Kim, Sang-Jong
    The journal of microbiology v.34 no.3 ,pp. 229 - 235 , 1996 , 1225-8873 ,

    초록

    Microgram quantities of DNA per gram soil were recovered with SDS- based and freeze-and thaw procedures. The average DNA fragment size was > 23 Kb. This method generated minimal shearing of extracted DNA. However, the DNA extracts still contained considerable amounts of humic impurities sufficient to inhibit PCR. Several approaches were used to reduce the interferences with the PCR (use of CTAF in extraction step, Elutip-d column purification, addition of BSA to PCR buffer) to accomplish PCR with DNA extract as a template. Most of the DNA extracts were not digested completely by restriction endonuclease, and CTAB-TREATED ane Elutip-d column purified DNA extracts were partially digested. Regarding as restriction enzyme digestion, all PCRs failed to amplify 16S rRNA gene fragments in the DNA extracts. In the case of DNA extracts only where BSA was added to PCR buffer, PCR was successfully conducted whether the DNA extracts were treated with CTAB or purified with columns. However, these two treatments were indispensable for humic impurity-rich DNA extracts to generate the PCR-compatible DNA samples. Direct extraction of DNA, coupled with these procedures to remove and relieve interferences by humic impurities and followed by the PCR, can be rapid and simple method for molecular microbiological study on soil microorganisms.

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  5. [국내논문]   Detection of Rifampin Resistance Mutation and Its Altered Nucleotide Sequences in Mycobacterium leprae Isolated from Korean Patients with Leprosy  

    Kim, Soon-Ok (Myong Ji University ) , Kim, Min-Joo (Catholic University Medical College ) , Tae, Chae-Gue (Catholic University Medical College ;) , Suh, Joo-Won
    The journal of microbiology v.34 no.3 ,pp. 236 - 240 , 1996 , 1225-8873 ,

    초록

    Rifampin is the most powerful drug for treating leprosy and tuberculosis today. It inhibits initiation and elongation of RNA transcription by binding to $\beta$ -subunit of RNA polymerase, leading to kill mycobacteria. We isolated one variant strain of Mycobacterium leprae from 24 Korean leprosy patients who are less susceptible to rifampin or have suffered from relapse by polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP) of the rpoB gene. Direct sequencing of the rpoB region of M. leprae variant revealed missense mutations which altered the amino acids sequenceof RpoB to Ser-464, Arg-465, Arg-467 and Ala-468. This is the first finding on rpoB gene mutation of M. leprae from Korean patients ; moreover the mutant type was found to be different from the previously reported cases in other countries.

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  6. [국내논문]   Structural and Functional Stability of the Genetic Recombinant Plasmid pCU103 in Different Water Environments   피인용횟수: 1

    Kim, Chi-Kyung (Chungbuk National University ) , Kwak, Myoung-Ja (Chungbuk National University ;) , Lee, Sung-Gie
    The journal of microbiology v.34 no.3 ,pp. 241 - 247 , 1996 , 1225-8873 ,

    초록

    The stability of the genetically engineered microorganisms and their recombinant plasmids released in natural environments has been regarded as one of the molecular ecological topics. In this study, the recombinant plasmids pCU103 in which the pcbCD genes involved in biodegradation of biphenyl and 4-chlorobiphenyl were cloned in pBluescript SK(+) vector, were examined for their structural and functional stability in different waters at 15 $^{\circ}C$ by the methods of electrophoresis, Southern hybridization, quantification with fluorescent dye, and transformation. The recombinant plamids maintained their stabilities for about 30 days in sterilized distilled water (SDW), 15 days in autoclaved creek water (AW), 25 days in filtered and autoclaved non-sterile creek water (FAW), 4 days in Luria-Bertani (LB) broth, and less than one day in filtered non-sterile creek water (FW). The covalently closed circular (CCC) form of the plasmid was decreased and open circular (OC) form was increased as a function of incubation time, and then linear (L) form was produced to be ultimately degraded out. The degradation rates of the plasmid were proportionally correlated to trophic level of the water, and the biological factor such as DNases was found to be one of the most critical factors affecting structural and functional stability of the plasmid in non-sterile natural water.

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  7. [국내논문]   Immunofluorescence Localization of Schizosaccharomjyces pombe $cdc103^{+}$ Gene Product  

    Kim, Hyong-Bai
    The journal of microbiology v.34 no.3 ,pp. 248 - 254 , 1996 , 1225-8873 ,

    초록

    $cdc103^+$ gene in Schizosaccharomyces pombe which is similar to the CDC3 gene in Saccharomyces cerevisiae was cloned and sequenced. Comparison of the predicted amino acid sequences of $cdc103^+$ and CDC3 revealed that they share significant similarity (43% identity and 56% identity or similarity) to each other. The gene product of CDC3 in S. cerevisiae is known to be a highly ordered ring of filaments that lies just inside the cytoplasmic membrane in the region of the mother-bud neck. In order to characterize the gene product of $cdc103^+$ in Schizosaccharomyces pombe, fusion proteins were used to generate the polyclonal antibodies specific for the gene product (cdc103p). In immunofluorescence experiments, these antibodies decorate the region of the septum formation as a double ring structure late in the cell division cycle.

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  8. [국내논문]   Calcium in infectious hematopoietic necrosis virus (IHNV) infected fish cell lines  

    Kim, Nam-Shik (Chungbuk National University ) , Heo, Gnag-Joon (Chungbuk National University ) , Lee, Chang-Hee (Chungbuk National University)
    The journal of microbiology v.34 no.3 ,pp. 253 - 269 , 1996 , 1225-8873 ,

    초록

    Infection of fish cells with IHNV resulted in gradual increase in cytosolic free Ca $\^$ 2+/ concentration ([Ca $\^$ 2+/)] in CHSE, gradual decrease in [Ca $\^$ 2+/] in FHM, and no significant change in RTG cells. The degree of [Ca $\^$ 2+/] increase or decrease was dependent on the amount of infectious virus, and these [Ca $\^$ 2+/] variations were maximal at 16 hours after virus infection (p. i.) in both cell lines. When the fish cells were infected with inactivated IHNV, evident variation in [Ca $\^$ 2+/] was not observed. Thus, infectivity of IHNV appears to correlate with changes in [Ca $\^$ 2+/] in virus-infected cells. These IHNV-induced [Ca $\^$ 2+/] changes were partially blocked by cycloheximide, but not affected by cordycepin. It seems to be that virus-induced Ca $\^$ 2+/ variations were more related with protein synthesis than RNA synthesis. Various Ca $\^$ 2+/ related drugs were used in search for the mechanisms of the [Ca $\^$ 2+/], changes following IHNV infection of CHSE cells. Decreasing extracellular Ca $\^$ 2+/ concentration or blocking Ca $\^$ 2+/ influx from extracellular media inhibited the IHNV-induced increase in [Ca $\^$ 2+/], in CHSE cells. Similar results were obtained with intracellular Ca $\^$ 2+/ sources are important in IHNV-induced [Ca $\^$ 2+/] increase in CHSE cells.

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  9. [국내논문]   Lipopolysaccharide Yields from Rhodobacter capasulatus with indirect ELISA  

    Yoo, Tae-Eun (Department of Biology Sung Kyun Kwan University ) , Lee, Hyun-Soon (Department of Biology Sung Kyun Kwan University)
    The journal of microbiology v.34 no.3 ,pp. 255 - 262 , 1996 , 1225-8873 ,

    초록

    The lipopolysaccharide (LPS) yields were measured in Rhodobacter capsulatus under several conditions by the ELISA method. The purification of LPS was done by affinity chromatography of IgG coupled CNBr-activated sepharose-4B instead of ultra-centrifugation. The purity of the LPS didn't show much difference between affinity chromatography and ultra-centrifugation method, but affinity chromatography method required much fewer organisms and was more convenient. LPS yield was measured in ng units by the ELISA method. Mannitol was a better single carbon source than other sugars, but mixing two carbon sources resulted in greater LPS yields than any sugar alone. LPS yield was directly proportional to $NH_ 4CI$ concentration, with optimum yields at 0.05% nitrogen. In contrest to LPS yields, which decreased at 0.005% nitrogen concentration total protein was increased 16 times. Calcium influenced LPS yields. At 0.7 mM $CaCI_ 2$ , the LPS yield was 16.5 $\mu$ g/mg DW, five times the yield without calcium.

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  10. [국내논문]   Inhibition of Purine Nucleoside Phosphorylase (PNP) in Micrococcus luteus by Phenylglyoxal  

    Choi, Hye-Seon
    The journal of microbiology v.34 no.3 ,pp. 270 - 273 , 1996 , 1225-8873 ,

    초록

    Micrococcus luteus purine nucleoside phosphorylase (PNP) has been purified and characterized. The physical and kinetic properties have been described previously. Chemical modification of the enzyme was attempted to gain insight on the active site. The enzyme was inactivated in a time-dependent manner by the arginine- specific modifying reagent phenylglyoxal. There was a linear relationship between the observed rate of inactivation and the phenylglyoxal concentration. At 30 $^{\circ}C$ the bimolecular rate constant for the modification was 0.015 $min^{-1}mM^{-1}$ in 50 mM $NaHCO_3$ buffer, pH 7.5. The plot of logk versus log phenylglyoxal concentration was a strainght line with a slope value of 0.9, indicating that modification of one arginine residue was needed to inactivate the enzyme. Preincubation with saturated solutions of substrates protected the enzyme from inhibition of phenylglyoxal, indicating that reactions with phenylglyoxal were directed at arginyl residues essential for the catalytic functioning of the enzyme.

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