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H : 소장처정보

T : 목차정보

The journal of microbiology 13건

  1. [국내논문]   Numerical Classification of Actinomycetes Isolated from Volcanic Soil  

    Kim, Seung-Bum (Research Center for Molecular Microbiology, Seoul National University ) , Lee, Soon-Dong (Research Center for Molecular Microbiology, Seoul National University ) , Kim, Seon-Young (Research Center for Molecular Microbiology, Seoul National University ) , Oh, Hyung-Myung (Research Center for Molecular Microbiology, Seoul National University ) , Kang, Sa-Ouk (Research Center for Molecular Microbiology, Seoul National University)
    The journal of microbiology v.34 no.2 ,pp. 105 - 116 , 1996 , 1225-8873 ,

    초록

    Of actinomycetes isolated from volcanic compost soils, 115 representative strains which showed distinctive morphologicla features were numerically classified, compared with reference strains of Streptomyces. One hundred and twenty unit characters were tested and the average probability of error was 4.27%. The cluster analysis resulted in two groups: group A included strains of actinomycetes except streptomycetes. Group A was divided into 2 major clusters (over 5 strains), 10-diaminopimelic acid. Group B was divided into 5 clusters, of which 4 clusters contained mesodiminopimelic acid and 1 cluster LL-diaminopimelic acid. The major clusters of group A showed higher abilities of substrate utilization and degradation, and higher resistance to inhibitors, whereas the minor and single member clusters of group A showed relatively higher antimicrobial activities. On the other hand, all clusters of group B showed relatively lower abilities of substrate utilization and degradation and lower resistance to inhibitors.

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  2. [국내논문]   Isolation and Identification of Infectious Pancreatic Necrosis Virus from Rainbow Trout Cultured in Korea  

    Lee, Jin-Hee (Department of Veterinary Medicine, Chungbuk National University ) , Heo, Gang-Joon (Department of Veterinary Medicine, Chungbuk National University ;) , Lee, Chan-Hee
    The journal of microbiology v.34 no.2 ,pp. 124 - 130 , 1996 , 1225-8873 ,

    초록

    A survey was conducted to determine the prevalance of infectious pancreatic necrosis virus (IPNV) on fish farms in Korea and the epidemiology of IPNV infection in the farmed rainbow trout. In total, 43 pools of rainbow trout with apparent signs of viral infection from five provinces were obtained and analyzed. Evident cytopathic effects, including karyopycnosis and cell destruction, were observed in CHSE (chinook samlmon embyro)-214 cells infected with the virus isolates. Of these, ten viral isolates were assumed to be IPNV based on biophysical properties. RNA analysis revealed that the isolates contained two-segmented RNA genomes, further indicating that the viral isolates are IPNV. Antigenic comparison of the IPNV isolates identified three distinct serological groups separable by the cross-neutralization test. Of the ten IPNV isolates, six could be classified as strain DRT, two as strain Ab, and two as strain VR299. We were not able to isolate new strain of IPNV or any isolate serologically similar to the standard strain Sp.poraceae and families of the Agaricales, they are genetically more related to the Polyporaceae. These results are consistent with morphological characters observed in those mushrooms. However, it is premature to conclude taxonomic status Ganoderma species in the present study employing small sample size.

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  3. [국내논문]   Distribution and activity of sulfate-reducing bacteria in lake soyang sediments  

    Jin, Hoo-Yong (Research Center for Molecular Microbiology, Seoul National University ) , Lee, Dong-Hun (Research Center for Molecular Microbiology, Seoul National University ) , Zo, Young-Gun (Research Center for Molecular Microbiology, Seoul National University ) , Kang, Chan-Su (Research Center for Molecular Microbiology, Seoul National University ;) , Kim, Sang-Jong
    The journal of microbiology v.34 no.2 ,pp. 131 - 136 , 1996 , 1225-8873 ,

    초록

    In order to known the extend of contribution to the degradation of organic materials and nutrient recycling by sulfate-reducing bacteria (SRB) and methane-producing bacteria (MPB) in sediment, the distribution and activity of these two groups of microorganisms were studied montly in 1994 at two sites, one littoral (Sanggulri) and the other profunndal (DAM), in Lake Soyang. In the seasonal distribution of two microorganisms, SRB were 1.07 $\times$ 10 $^{3}$ -2.42 $\times$ 10 $^{5}$ cells/g-dry weight at Sanggulri, 2.40 $\times$ 10 $^{5}$ -1.29 $\times$ 10 $^{6}$ at Dam and MPB were 0.52 $\times$ 10 $^{3}$ cells/g-dry weight at Sangguri and 1.44 $\times$ 10 $^{3}$ -6.89 $\times$ 10 $^{3}$ at Dam. In these results, the density of SRB in Lake Soyang is much higher than other lakes. These high values might be due to higher sulfate concentration, 0.69-4.05 mM, than normal freshwater, 0.01-1.2 mM. And a good correlation of SRB and chlorophyll a concentration implied that the important environmental factor on distribution of SRB might be the concentration of available organic matter. In a comparison of sulfate-reducing rate and methane producing rate in 1995, the activity of SRB for the degradation of organic matter was higher than MPB by factor of 359. Conclusively SRB superior to MPB in the distribution and activity are more important annearobic bacteria in Lake Soyang sediments.

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  4. [국내논문]   Regulation of fpr Gene Encoding NADPH : Ferredoxin Oxidoreductase by the soxRS Locus in Escherichia coli  

    Koh, Young-Sang (Research Center for Molecular Microbiology, Seoul National University ) , Choih, Jenny (Research Center for Molecular Microbiology, Seoul National University ;) , Roe, Jung-Hye
    The journal of microbiology v.34 no.2 ,pp. 137 - 143 , 1996 , 1225-8873 ,

    초록

    We isolated a promoter inducible by paraquat, a superoxide-generating agent, from Escherichia coli using a promoter-probing plasmid pRS415. From sequence analysis we found out the promoter is for fpr ENCODING nadph : ferredoxin oxidoreductase. We constructed on operon fusion of lacZ gene with fpr promoter to monitor the expression of the gene in the single-copy state. LacZ expression generators, menadione and plumbagin, also induced the expression of .betha.-galactosidase in the fusion strain. On the other hand, no significant induction was observed by treatment with hydrogen peroxide, ethanol, and heat shock. Induction of .betha.-galactosidase was significantly reduced by introducing a .DELTA. sox 8 :: cat of soxS3 :: Tn10 mutation into the fusion strain, indicating that fpr gene is a member of the soxRS regulon. The transcriptional start site was determined by primer extension analysis. Possible roles of fpr induction in superoxide stress were discussed.

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  5. [국내논문]   Extracellular DNAs Released form the Genetically Engineered E. coli CU103 During Growth in Different Liquid Media  

    Kim, Chi-Kyung (Department of Microbilogy, Chungbuk National University ) , Park, Sang-Ho (Department of Microbilogy, Chungbuk National University ) , Lim, Jai-Yun (Department of Microbilogy, Chungbuk National University ) , Kim, Young-Chang (Department of Microbilogy, Chungbuk National University ) , Kim, Youngsoo (Department of Pharmacy, Chungbuk National University ) , Min, Kyung-Hee (Department of Biology, Sookmyong Womens University ) , Lee, Ki-Sung (Department of Biology, Pai-Chai University)
    The journal of microbiology v.34 no.2 ,pp. 144 - 150 , 1996 , 1225-8873 ,

    초록

    During growth of the genetically engineered E. coli CU103 in different media, extracellular DNAs released from the cells were studied. The extracellular DNAs released in the medium were concentrated by an thanol precipitation method and then quantified by a fluorescence method using Hoechst 33258. The released extracellular DNAs were also examined by gel electrophoresis and identified by Southern hybridization for the cloned pcbCD genes. The chromosomal DNAs and recombinant plasmid containing the cloned genes were observed to be released in an exponential growth phase. In Luria-Bertani (LB) broth and MM2-GLUCOSE, 210 and 69 ng/ml of DNAs were detected, respectively, after 3-4 days incubation at $30^{\circ}C$ and at pH 7.0. But the released DNAs were measured to be about 10-15 ng/ml in filtered river water (FW) and Tris-EDTA (TE). The at both $15^{\circ}C$ and $4^{\circ}C$ , but the released DNAs were more easily degraded at the higher temperature. The extracellular DNAs were produced about 2 times more at pH 7.0 than at both pH 5.0 and pH 9.0 in MM2-glucose medium at $30^{\circ}C$ . Therefore, the extracellular DNAs were found to be released actively from the cells during growth in liquid media.

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  6. [국내논문]   Genetic Relationship between the SPT3 Gene and RAS/cAMP Pathway in Yeast Cell Cycle Control  

    Shin, Deug-Yong (Korea Research Institute of Bioscience and Biotechnology ;) , Yun, Jean-Ho
    The journal of microbiology v.34 no.2 ,pp. 158 - 165 , 1996 , 1225-8873 ,

    초록

    The signal transduction pathways through the RAS gene product and adenyl cyclease play a critical role in regulation of the cell cycle in yeast, Saccharomyces cerevisiae. We examined the genetic relationship between the spt3 gene and ras/cAMP pathway. A mutation in the SPT3 gene suppressed cell cycle arrest at the G1 phase caused by either an inactivation of the RAS or CYR1 gene which encodes a yeast homologue of human ras proto-oncogene or adenyl cyclase, respectively. The phenotypes such as sporulation and heat shock resistancy, that resulted from a partial inactivation of the RAS or CYR1 genes, were also suppressed by the spt3 mutation. Expression of the SSA1 gene encoding one of th heat shock proteins (Hsp70) can be induced by heat shock or nitrogen starvation. Expression of this gene is derepressed in cry1-2 and spt3 mutants. The bcy 1 mutation repressed by the bcy1 mutation, but not in spt3 mutants. These results suggest that the SPT gene is involved in expression of genes that are affected by the RAS/cAMP pathway.

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  7. [국내논문]   Fast Genetic Variation among Coliphage Quasispecies Revealed by a Random Amplified Polymorphic DNA (RAPD) Analysis   피인용횟수: 1

    Kwon, Oh-Sik (Department of Biology Keimyung University ) , Lee, Jae-Yung (Department of Biology, Mokpo National University)
    The journal of microbiology v.34 no.2 ,pp. 166 - 171 , 1996 , 1225-8873 ,

    초록

    Genetic analysis was conducted on newly isolated coliphages form soil by using a RAPD assay. From the initial result, the coliphages were turned out to be different form one another but were closely related to .psi..lambda. due to the fact that they shared the samed RAPD maker in which other T phage testings failed to show. By using the primers EC01 or EC02, a fast genetic mutation of .psi.C1 was found by producing specific RAPD markers on the phages from the first filial progeny to the second filial progeny. When we made a RAPD assay with combined primers (EC01, EC05 and EC08), the genetic mutation was again confirmed in .psi.C1. The assay detection showed mutations in other coliphages such as .psi.C2 and .psi.C3 by revealing specific RAPD bands among different progeny phages, where genetic instability of the coliphages in implied.

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  8. [국내논문]   Effect of Copper on the Growth and Methanol Dehydrogenase Activity of Methylobacillus sp. Strain SK1 DSM 8269  

    Kim, Si W. (Dept. of Biology, Yonsei Univ. ) , Kim, Young M. (Dept. of Biology, Chosun University)
    The journal of microbiology v.34 no.2 ,pp. 172 - 178 , 1996 , 1225-8873 ,

    초록

    Methylobacillus sp. strain SK1, which grows only on methanol, was found to grow in the absence of added copper. The doubling time (t $_{d}$ = 1.3 1.3 h) of the bacterium growing at the exponential growth phase at 30.deg.C in the absence of copper was the same as that of the cell growing in the presence of copper. The bacterium growing after the exponential phase in the absence of copper, however, grew faster than the cell growing in the presence of copper. Cells harvested after thee arly stationary phase in the presence of copper were found to exhibit no methanol dehydrogenase (MDH) activity, but the amount and subunit structure of the enzyme in the cells were almost the same as that in cells harboring active MDH. Pellets of the cells harvested after the early stationary phase in the presence of copper were pale green. Cell-free extracts prepared from cells harvested at the early stationary phase in the presence of copper were pink and exhibited MDH activity, but it turned dark-green rapidly from the surface under air. The green-colored portions of the extracts showed no MDH activity and contained c-type cytochromes that were oxidized completely. The inactive MDH activity and contained c-type cytochromes that were oxidized completely. The inactive MDH proteins in the green portions were found to have antigenic sites identical to those of the active one as the inactive MDHs in cells grown in the presence of copper. The bacterium was found to accumulate copper actively during the exponential growth phase. MDH prepared from cells grown in the presence or absence of copper was found to be more stable under nitrogen gas than under air. Methanol at 10 mM was found to enhance the stability of the MDH under air.r.

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  9. [국내논문]   Optimization of Culture Conditions for Production of Pneumococcal Capsular Polysaccharide Type I  

    Kim, Su-Nam (Department of Pharmacy, SungKyunKwan University ) , Min, Kwan-Ki (Department of Pharmacy, SungKyunKwan University ) , Kim, Seung-Hwan (Department of Pharmacy, SungKyunKwan University ) , Choi, In-Hwa (Department of Pharmacy, SungKyunKwan University ) , Lee, Suhk-Hyung (Department of Pharmacy, SungKyunKwan University ) , Pyo, Suhk-Noung (Department of Pharmacy, SungKyunKwan University ) , Rhee, Dong-Kwon (Department of Pharmacy, SungKyunKwan University)
    The journal of microbiology v.34 no.2 ,pp. 179 - 183 , 1996 , 1225-8873 ,

    초록

    Streptoccus Pneumoniae (pneumococcus), the most common cause of bacterial pneumonia, has an ample polysaccharide (PS) capsule that is highly antigenic and is the source of PS vaccine. This investigation was undertaken to optimize the culture conditions for the production of capsulard PS by type 1 pneumococcus. Among several culture media, brain heart infusion (BHI) and Casitone based media were found to support luxuriant growth of pneumococcus type 1 at the same level. Because BHI medium is rather expensive and more complex than the Casitone based media, the Casitone based media was uwed to study optimization of the culture condition. The phase of growth which accomodated maximum PS production was logarithmic phase. Concentrations of glucose greater than 0.2% did not ehnahce growth or PS production. Substitution of netrogen sources with other resources or supplementation of various concentrations of metal ion (with the exception of calcium ion) had adverse affects on growth and PS production. On the other hand, low level aeration was beneficial for increased PS production. Addition of 3 mg/1 concentration of methionine, phenylalanine, and threonine were found to enhance growth and PS production. The synerigistic effect of all the favorable conditions observed in pneumococcal growth assays provided a two-fold cummulative increase in capsular PS production.

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  10. [국내논문]   Lipid analysis of streptomycetes isolated form volcanic soil   피인용횟수: 1

    Kim, Seung-Bum (Research Center for Molecular Microbiology, Seoul National University ) , Kim, Min-Young (Research Center for Molecular Microbiology, Seoul National University ) , Seong, Chi-Nam (Suncheon National University ) , Ouk, Kang-Sa (Research Center for Molecular Microbiology, Seoul National University ;) , Hah, Yung-Chil
    The journal of microbiology v.34 no.2 ,pp. 184 - 191 , 1996 , 1225-8873 ,

    초록

    The cellular fatty acids and quinones of streptomycetes isolated from volcanic soils were analysed. The strains contained fatty acids of 14 to 17 carbon chains, and 12-methyltetradecanoic acid and 14 methylpentadecanoic acid were dominant in most strains. The total profiles consisted of 74% branched fatty acid family, 16.8% linear family and 8.2% unsaturated family. The largest cluster of grey spore meases defined by numerical classification was separated from the remainders in the principal component analysis, but the other clusters were overlapped with one another. In the analysis of respiratory quinones, all of the strains contained either the menaquinone of 9 isoprene units with 6 hydrogenations of 8 hydrogenations as the major species. The distribution of menaquinones among the clusters could provide an important key in the chemotaxonomy of streptomycetes.

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