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Journal of food science and nutrition 15건

  1. [국내논문]   Isolation and Identification of Anthocyanins from Purple Sweet Potatoes   피인용횟수: 1

    Lee, Lan-Sook (Dept. of Food Engineering, Mokpo National University ) , Chang, Eun-Ju (Dept. of Food Science and Nutrition, Catholic University of Taegu-Hyosung ) , Rhim, Jong-Whan (Dept. of Food Engineering, Mokpo National University ) , Ko, Byoung-Seob (Korea Institute of Oriental Medicine ) , Park, Sang-Won (Dept. of Food Science and Nutrition, Catholic University of Taegu-Hyosung)
    Journal of food science and nutrition v.2 no.2 ,pp. 83 - 88 , 1997 , 1226-332x ,

    초록

    Anthocyanin pigments of purple sweet potato roots (Ipomoea batatas L.) were extracted with 0.5% TFA (Trifluoroacetic acid) in 95% EtOh, and further isolated an purified by Amberlite XAD-7 and ODS column chromatography, and final preparative HPLC. Among nine anthocyanins isolated, the structure of three major anthocyanins were identified as 3-O-(6-O-trans-caffeyl)-2-O-(6-O-trans-caffeylglucopyranosyl)- $\beta$ -D-glucopyranosyl-5-O-( $\beta$ -D-glucopyranosyl)-peonidin, 3-O-(6-O-trans-caffeyl)-2-O-(6-O-trans-feruloyl-glucopyranosyl)- $\beta$ -D-glucopyranosyl-5-O-( $\beta$ -D-glucopyranosyl)-peonidin, and 3-O-(6-O-trans-caffeyl)-2-O-(6-O-p-hydroxylbenzoylglucopyranosyl)- $\beta$ -D-glucopyranosyl)-peonidin, by using UV-visible absorption spectra, $^1$ H-NMR and FAB-MS analysis.

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  2. [국내논문]   Effects of Ionizing Energy and Ozone Treatments on the Microbial Decontamination and Physicochemical Properties of Aloe Powders and Bee Pollen  

    Yook, Hong-Sun (Dept. of Food Irradiation, Korea Atomic Energy Research Institute ) , Chung, Young-Jin (Dept of Food and Nutrition, Chungnam National University ) , Kim, Jung-Ok (Dept. Home Economics, King Sejong University ) , Kwon, Oh-Jin (Dept. of Food Irradiation, Korea Atomic Energy Research Institute ) , Kim, Sung (Dept. of Food Irradiation, Korea Atomic Energy Research Institute ) , Byun, Myung-Woo (Dept. of Food Irradiation, Korea Atomic Energy Research Institute)
    Journal of food science and nutrition v.2 no.2 ,pp. 89 - 95 , 1997 , 1226-332x ,

    초록

    The comparative effects of gamma irradiation an ozone treatment on the microbiological and physicochemical qualities were investigated for the improvement of hygienic quality of aloe powder and bee pollen. Gamma irradiation at 7.5~10kGy could reduce total aerobic bacteria, molds and coliforms below detection levels, but ozone treatment up to 18 ppm for 8hr was not sufficient to eliminate the microorganisms from aloe powder and bee pollen. The physicochemical properties such as fatty acid an amino acid compositions, mineral content, TBA value, barbaloin and pigment contents were not significantly changed by gamma irradiation, whereas ozone treatment caused significant changes in fatty acid composition, lipid oxidation and destruction of barbaloin and natural pigments.

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  3. [국내논문]   Lack of the Initiation of Benzo[a]pyrene-induced Mouse Forestomach Neoplasia by Di(2-ethylhexyl)phthalate(DEHP)  

    Lee, Sang-Ho (Institute of Haitai Confectionary Co., LTD ) , Le, Young-Chun (Dept. of Food Technology, Chungang University ) , Kim, Jeong-Ok (H&K Laboratories ) , Ha, Yeong-Lae (Dept. of Agricultural Chemistry, Gyeongsang National University)
    Journal of food science and nutrition v.2 no.2 ,pp. 96 - 100 , 1997 , 1226-332x ,

    초록

    Carcinogenicity of di(2-ethylhexyl)phthalate(DEHP) to the mose forestomach and its inhibitor activity for the initiation of Benzo[a]pyrene(BP)-induced mouse forestomach neoplasia were studied on the mouse forestomach carcinogenesis regimen. One hundred female ICR mice(6~7 weeks of age) were hosed in a poly-carbonate cage (4 mice/cage) in a humidity- and temperature-controlled room subjected to a semipurified diet for a week. Mice were divided into 4 treatment groups (25 mice/treatment): Basal diet, DEHP, BP, and BP+DEHP. On Monday and wednesday, 0.1ML DEHP mixed with 0.1ml olive oil (for DEHP and DEHP+BP treatment groups) or 0.1ml saline+0.1ml olive oil (for basal diet group) was intubated, p.o., and on Friday, 2mg BP dissolved in 0.2ml olive oil (for BP and BP+DEHP treatment groups) was intubated, p.o. This cycle was repeated for 4 weeks. Beginning with the first intubation of BP an continuing thereafter, body weight and food intake were recorded once and twice weekly, respectively. All surviving mice were sacrificed 22 weeks after the first dose of BP intubation and countered forestomach tumor. No tumor was formed by DEHP treatment. 5.75 tumors per mouse was formed by BP treatment, whereas its number was reduced to 4.53 by BP+DEHP treatment. Similar results were seen in the tumor incidence. Body weight gain was not affected by DEHP treatment, when compared to that b basal diet treatment. The body weight was significantly reduced by BP treatment, but its reduction was recovered to the level of the basal diet group by BP+DEHP treatment. No significant difference was seen in food intake among all treatment groups. These results indicate that DEHP lacks carcinogenic activity to the mose forestomach and rather inhibits the initiation of BP-induced mose forestomach neoplasia.

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  4. [국내논문]   Characterization of Bacteriocin Production by Lactococcus lactis LAB3113 Isolated from Kimchi  

    Jong-Yeun Shin , Cheol Ahn
    Journal of food science and nutrition v.2 no.2 ,pp. 101 - 108 , 1997 , 1226-332x ,

    초록

    A lactic acid bacterium, LAB3113, isolated from traditionally fermented Kimchi was found to produce bacteriocin whose activity was very specific toward lactobacilli and not effective against any other bacteria. Lactobacilli affected by the inhibitory substance included Lactobacillus delbrueckii-lactis, L. johnsonii, L. gasseri, and L. curvatus. Based upon biochemical and physiological characteristics, LAB3113 was classified as Lactococcus lactis, and its bacteriocin was named as lactococcin K3113. Lactococcus lactis. LAB3113 produced bacteriocin at the early stage of growth and the concentration of the bacteriocin did not decrease even after late stationary phase. Optimal temperature of bacteriocin production was 25℃ at the initial pH 7.0. Partially purified lactococcin K3113 was completely inactivated by protease, but not affected by lipase, lysozyme and RNase. The bacteriocin was very heat-stable even after autoclaving for 20min. It was also stable in pH changes, and was not affected by the presence of solvents. Lactococcin K3113 appeared to act in bactericidal mode against L. delbrueckii-lactis ATCC4797. Molecular weight of lactococcin K3113 was calibrated as 10,500dal by SDS-PAGE and activity staining. Lactococcus lactis LAB3113 had four residential plasmids of 3.7kb, 11.2kb, 15.5kb, and 48kb in molecular sizes. Plasmid profile analysis of mutant strain revealed that 15.5kb plasmid was re-sponsible for the production of lactococcin K3113 and its immunity to the bacteriocin.

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  5. [국내논문]   Antibiosis and Bacteriocin Production of Lactic Acid Bacteria Isolated from Kimchi  

    Sung-Sook Bae , Cheol Ahn
    Journal of food science and nutrition v.2 no.2 ,pp. 109 - 120 , 1997 , 1226-332x ,

    초록

    In other to elucidate roles of lactic acid bacteria(LAB) for the antibiosis occurring in the fermenting environment of Kimchi, 2,052 strains of LAB were isolated from Kimchi. Fifty two strains which showed antagonistic effect against 4 indicator strains were finally selected and investigated. Based upon responses to protease treatment, antibiosis of the 52 strains of LAB were classified into 3 types. Type A antibiosis resulted from action of antibiotic-like substances which were not affected by protease treatment and which had broad action spectra against even natural inhabitants of Kimchi. Type B antibiosis was due to bacteriocinlike substances which were very sensitive to treatment of protease and more effective against foreign bacteria than original inhabitant microflora. Type C antibiosis was owing to proteinaceous compounds which were activated or induced by the presence of protease and then exerted antibacterial activities. Therefore, lactic acid bacteria appeared to contribute to antibiosis of Kimchi by the concerted action of these three different types of antibacterial compounds. As one of model system for type B bacteriocin, the antagonistic compound produced by LAB31-9 as well as the producer strain itself was further characterized. Strain LAB31-9 was identified as L. casei. Bacteriocin produced by LAB31-9 was proteinaceous and stable over wide range of pH and to various solvents, but very labile to heat treatment. Its mode of action was bactericidal. Based upon these data, bacteriocin produced by LAB31-9 was named as 'caseicin K319'. Genetic determinant for the bacteriocin production of LAB31-9 was located in the chromosome.

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  6. [국내논문]   Effects of High Cholesterol Feeding on Regulation of Plasma Lipids and Reverse Cholesterol Transport in Rabbits  

    Park, Myung-Sook (Dept. of Food Science and Nutrition, Kyungpook National University ) , Seo, Jin-Ah (Dept. of Food Science and Nutrition, Kyungpook National University ) , Cho, Kyung-Hyun (Dept. of Genetic Engineering, Kyngpook National niversity ) , Bok, Song-Hae (Bioproducts Research Group, Genetic Engneering Research Institute ) , Park, Yong-Bok (Dept. of Genetic Engineering, Kyngpook National University)
    Journal of food science and nutrition v.2 no.2 ,pp. 121 - 128 , 1997 , 1226-332x ,

    초록

    this study was conducted to examine the atherogenic effect of high cholesterol diet (experimental diet) that influences changes of lipoprotein cholesterol metabolism and arterial wall. Seven NewZealand white rabbits were fed control diet, an the other 7 rabbits 2% cholesterol diet for 10 weeks. Results obtained from this study are as follows: 1) High cholesterol diet resulted in a gradual increase of plasma total cholesterol level, reaching upto 1422 mg/dl at the seventh week. 2) CETP (cholesteryl ester transfer protein) activity was significantly higher in high cholesterol group (64.9% at the 7th week) than control group (49.3% at the 7th week) during most of the experimental period except the 6th week. 3) The cholesterol supplementation induced fatty liver and a decrease of hepatic HMG-CoA reductase activities (2.1 moles vs. 0.3nmoles) compared to control group. 4) Bands of apo B-100 and apo E in plasma lipoprotein were thicker in high cholesterol-fed animals tan control animals as visualized by SDS-PAGE. 5) Oxidizability of plasma lipoproteins measured in vitro was greater in high cholesterol group tan control group, but vitamin E level higher in control group. 6) he effect of cholesterol feeding for 10 weeks also led to early fatty streaks in aortic intima. High cholesterol feeding was atherogenic to rabbits, an this seems to be mediated through elevated CETP activities that regulate plasma HDL cholesterol level and decrease an efficiency of reverse cholesterol transport in lipoprotein cholesterol metabolism. The enhanced oxidizability of plasma lipoproteins and lowered vitamin E level may also contribute to the formation of faaty streaks in aorta of cholesterol-fed rabbits.

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  7. [국내논문]   Effect of Soybean Intake on Nutritional Status of the Healthy Elderly  

    Park, Yaung-Ja (Dept. of Food and Nutrition, Seoul National University ) , Rhie, Seung-Gyo (Dept. of Food and Nutrition, The University of Suwon)
    Journal of food science and nutrition v.2 no.2 ,pp. 129 - 137 , 1997 , 1226-332x ,

    초록

    The purpose of this study was to develop the dietary enhancement program for rural elderly. The subjects consisted of 71 normal healthy elderly aged over 60 living in rural and senior citizen center. Daily supplementation of 20g soybean powder of 3 months revealed no statistically significant elevation in serum parameters of the elderly, but resulted in the increment in the number of the subject with normal range of serum parameters and the improvement in perceptions of health status by CMI score, even though the nutrients intake had constantly lowered. The nutrients intake of rural elderly was very low: energy intake was 59-68% of RDA, that of protein 47~59%, and clacium 21~60% only. Supplementation of 20 g soybean powder per day for 3 months, did not enhance nutrients intake of rural elderly. We can recommended that constant nutrients supplementation program as well as dietary enhancement program are needed to improve the quality of life of rural elderly.

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  8. [국내논문]   Biological Effects of Korean Puerariae Radix Catechins on the Liver Function in Rats Administrated with Ethanol   피인용횟수: 1

    Lee, Chi-Ho (Animal Resources Research Center, College of Animal Husbandry at Kon-Kuk University)
    Journal of food science and nutrition v.2 no.2 ,pp. 138 - 143 , 1997 , 1226-332x ,

    초록

    Crude catechin extracts were prepared using ethyl acetate from Korean Puerariae Radix (PR) and their biological effects on the alcoholic liver damage were investigated in male Sprague-Dawley rats (6 weeks old). Ethanol (5g ethanol/kg body weight/day) administration was performed for 8 weeks and after ethanol consuming rats were treated with one or two% catechin extracts of diet for 8 weeks. At the end of experimental period, lipid hydroperoxides in liver were analyzed using a chemiluminescence-high performance liquid chromatography (CL-HPLC) method. Compared with control animals, ethanol consumed rats showed lighter body weights, lower ratios of liver/body weight, higher activities of GOT and GPT, and increased lipid hydroperoxide amount in liver. With one or two% catechin extracts treatment, GOT and GPT activities returned to normal ranges. Lipid hydroperoxide contents in liver of on or two% PR treated rats lowered to 20% or 25% respectively, compared with the levels of those ethanol consumed animals without catechin extracts treatment. Therefore, we concluded that on or two% PR crude catechins treatment could be effective for alcoholic liver damage caused by lipid peroxidation.

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  9. [국내논문]   Calcium Modulation of Insulin Secretion in Perfused Pancreata of Obese Zucker Rats  

    Park, Hyun-Ju (Dept. of Human Nutrition and Food Science, Chungnam Sanup University)
    Journal of food science and nutrition v.2 no.2 ,pp. 144 - 148 , 1997 , 1226-332x ,

    초록

    Insulin secretory response to various calcium concentrations was investigated in 10- to 12-week old male lean and obese Zucker rats using an in vitro pancreatic perfusion procedure. There was no significant difference in insulin secretion response to low, medium, and high calcium concentrations in the lean rat. However, the obese rat shows a characteristics of hypersecretion of insulin. The obese rat pancreas perfused with the low calcium concentration released as low insulin as the lean rat. When perfused with the medium calcium concentration, th obese rat pancreas released twice as much insulin as the lean rat. eh hypersecretory phenomenon was also seen in the obese rat pancreas perfused with the high calcium concentration during the first phase of erfusion period, but his phenomenon was gradually diminished during he second phase of perfusion period. These results indicate that there may be a selective insulin secretory response to the extracellular calcium in he obese Zucker rat pancreas.

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  10. [국내논문]   Effects of Exercise on Pasma Glucose and Cholesterol Level in Streptozotocin-induced Diabetic Rats Fed Buckwheat Diet  

    Park, Hyun-Ju (Dept. of Human Nutrition and Food Science, Chungnam Sanup University ) , Lee, Kyung-Hea (Dept. of Food and Nutrition, Changwon National University)
    Journal of food science and nutrition v.2 no.2 ,pp. 149 - 154 , 1997 , 1226-332x ,

    초록

    The ability of exercise and buckwheat diet to modify plasma glucose and cholesterol levels in streptozotocin-induced diabetic rats has been studied. Diabetic rats were fed corn starch as a control diet or buckwheat as an experimental diet for 4 weeks. One group of rats were exercise-trained to swim for 60 min/day, 6 days a week. Plasma glucose levels of sedentary rats both on the control diet and on the buckwheat diet were significantly increased from 367.0 $\pm$ 33.6 mg/dl to 545.0 $\pm$ 38.7 mg/dl and from 380.3 $\pm$ 18.9 mg/dl to 540.5 $\pm$ 36.6 mg/dl, respectively. However, this large increase of plasma glucose was not seen in exercised rats on the buckwheat diet (from 345.0 $\pm$ 25.6 mg/dl to 391.4 $\pm$ 34.7 mg/dl). The total plasma cholesterol level was not affected by either diet or exercise. The HDL-cholesterol level tends to increase due to the buckwheat diet or an exercise, but not with a significant increase. Our results suggest that the buckwheat diet is beneficial in lowering the plasma glucose level only when diabetic rats ar exercised.

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