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Journal of photoscience : an international journal... 8건

  1. [국내논문]   Photokinesis of Cyanobacterium Synechocystis sp. PCC 6803  

    Chung, Young-Ho (Proteome Analysis Team, Korea Basic Science Institute ) , Park, Young-Mok (Proteome Analysis Team, Korea Basic Science Institute ) , Moon, Yoon-Jung (Proteome Analysis Team, Korea Basic Science Institute ) , Lee, Eun-Mi (Proteome Analysis Team, Korea Basic Science Institute ) , Choi, Jong-Soon (Proteome Analysis Team, Korea Basic Science Institute)
    Journal of photoscience : an international journal officail organ of the korean society of photoscience v.11 no.3 ,pp. 89 - 94 , 2004 , 1225-8555 ,

    초록

    Motile cyanobacterium Synechocystis sp. PCC 6803 cells show photomovement with respect to the light stimulus. Under lateral irradiation, Synechocystis displays a phototactic gliding movement toward the light source by a twodimensional random biased walk. Under vertical irradiation, Synechocystis decreased the frequency of mean vectorial gliding speed dependent on the applied fluence rate, whereas the deviation distribution width of the speed increased. This strongly suggests the involvement of photokinesis. Evidence for the cyanobacterial photokinesis was discussed in the previous report (Choi et al., 1999. Photochem. Photobiol. 70, 95-102) demonstrating that the gross scalar speed of vertically irradiating cells increased by about 50% compared with that of dark-adapted cells. In the visible wavelength range, Synechocystis cells showed a maximal photokinetic activity at 420 nm and a second maximal activity at 680 nm. The threshold action spectrum for the photokinesis resembles the absorption spectrum of chlorophyll with major differences in the phototaxis action spectrum at 560 nm and 660 nm. We postulate that the cyanobacterial photokinesis is powered by the energy-generating chlorophyll pigments.

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  2. [국내논문]   Effect of Deuterated Solvents on the Excited State Photophysical Properties of Curcumin  

    Barik, A. (Radiation Chemistry & Chemical Dynamics Division, Bhabha Atomic Research Center ) , Goel, N.K. (Radiation Technology Development Section, Bhabha Atomic Research Center ) , Priyadarsini, K.I. (Radiation Chemistry & Chemical Dynamics Division, Bhabha Atomic Research Center ) , Mohan, Hari (Radiation Chemistry & Chemical Dynamics Division, Bhabha Atomic Research Center)
    Journal of photoscience : an international journal officail organ of the korean society of photoscience v.11 no.3 ,pp. 95 - 99 , 2004 , 1225-8555 ,

    초록

    Optical absorption and emission studies have been carried out to understand the effect of deuterium on the solvent dependent photophysical properties of curcumin in deuterated solvents such as $CDCl_3,\;(CD_3)_2SO,\;(CD_3)_2CO,\;CD_3OD\;and\;CD_3CN$ . Optical absorption spectral studies showed that there is no significant shift in absorption maxima compared to the non-deuterated solvent. The fluorescence maxima shows significant shift with polarity of solvent but not much affected by the deuteration. The fluorescence quantum yield of curcumin increased marginally in almost all the deuterated solvents, indicating reduction in the non-radiative pathways. The fluorescence decay was biexponential in all the solvents and the average fluorescence lifetime was not much affected with deuteration, but showed decrease with increasing solvent polarity. Based on these studies, it is concluded that intermolecular hydrogen transfer is only partially responsible for the excited state deactivation of curcumin.

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  3. [국내논문]   Synthesis, Fabrication and Characterization of Photorefractive Composite Utilizing Flexible NLO Chromophores and Crosslinkable Photoconducting Matrix  

    Suh, Sang-Chul (Department of Chemistry, Korea Advanced Institute of Science and Technology ) , Shim, Sang-Chul (Department of Chemistry, Korea Advanced Institute of Science and Technology ) , Kim, Ae-Rhan (Department of Chemistry, Chonbuk National University ) , Yoo, Dong-Jin (Department of Chemistry, Seonam University)
    Journal of photoscience : an international journal officail organ of the korean society of photoscience v.11 no.3 ,pp. 101 - 106 , 2004 , 1225-8555 ,

    초록

    The photorefractive composites fabricated by blending NLO chromophores such as 3-(4-{bis-[4-(2-ethylhexyloxy)-phenyl]-amino}-phenyl)-2-isocyano-acrylonitrile (PAIA), bis-[4-(2-ethyl-hexyloxy)-phenyl]-{4-[2-(4-nitrophenyl)-vinyl]-phenyl}-amine (PNPA) with photoconducting crosslinkable matrix, bis-(4-ethynylphenyl)-(4-octyloxyphenyl)-amine (BEOPEA), showed photorefractive property due to the high composition of NLO chromophores caused by introducing alkoxy chain, and many problems faced in typical organic photorefractive systems such as timeconsuming chemical synthesis, difficulty in rational design, intrinsic instability and phase separation could be avoided.

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  4. [국내논문]   Absorption and Fluorescence Studies of 3-Ethenylindoles  

    Singh, Anil K. (Department of Chemistry, Indian Institute of Technology ) , Hota, Prasanta K. (Department of Chemistry, Indian Institute of Technology)
    Journal of photoscience : an international journal officail organ of the korean society of photoscience v.11 no.3 ,pp. 107 - 113 , 2004 , 1225-8555 ,

    초록

    Synthesis, absorption and fluorescence properties of 3-methyl indole (1), N-(benzenesulfonyl)-3-(3-oxo-but-1-enyl)-indole (2) and 1H-3-(3-oxo-but-1-enyl)-indole (3) are described. Extended conjugation at C-3 of indole as in 3 causes moderate resolution of $^1L_a$ and $^1L_b$ bands. However, 2 having an electron-withdrawing group at indolic nitrogen shows only the $^1L_a$ band. While the $^1L_b$ band largely remains solvent polarity independent, the $^1L_a$ band undergoes moderate red shift in polar solvents. The fluorescence in 2 and 3 originates from the $L_b$ transition. Additionally, interaction of 2 and 3 with BSA indicates that these compounds bind to the hydrophobic site of BSA with the formation of a highly fluorescent BSA-probe complex.

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  5. [국내논문]   Construction of Gene-Specific Primers for Various Antioxidant Isoenzyme Genes and Their Expressions in Rice (Oryza sativa L.) Seedlings Obtained from Gamma-irradiated Seeds  

    Kim, Jin-Hon (Division of Radiation Application Research, Korea Atomic Energy Research Institute ) , Chung, Byung-Yeoup (Division of Radiation Application Research, Korea Atomic Energy Research Institute ) , Kim, Jae-Sung (Division of Radiation Application Research, Korea Atomic Energy Research Institute ) , Wi, Seung-Gon (Division of Radiation Application Research, Korea Atomic Energy Research Institute ) , Yang, Dae-Hwa (Division of Radiation Application Research, Korea Atomic Energy Research Institute ) , Lee, Choon-Hwan (Department of Molecular Biology, Pusan National University ) , Lee, Myung-Chul (National Institute of Agricultural Biotechnology, R.D.A.)
    Journal of photoscience : an international journal officail organ of the korean society of photoscience v.11 no.3 ,pp. 115 - 120 , 2004 , 1225-8555 ,

    초록

    For the expression study of antioxidant isoenzyme genes in rice (Oryza sativa L.) plants, extensive searches for genes of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) isoforms were performed through the GenBank database. The genes for two cytosolic and one plastidic CuZn-SOD, one Fe-SOD, two Mn-SOD, two cytosolic and two chloroplastic (stromal and thylakoid) APX, and three CAT isoforms were available in japonica-type rice. These isoforms were named as cCuZn-SOD1, cCuZn-SOD2, pCuZn-SOD, Fe-SOD, Mn-SOD1, Mn-SOD2, cAPXa, cAPXb, Chl_sAPX, Chl_tAPX, CATa, CATb, and CATc, respectively. Since they shared a high degree of homology in the nucleotide and amino acid sequences, the gene-specific primers for the genes were designed directly from their full-length cDNAs found in the database except for the CATa gene. These primers were used in the RT-PCR analysis to investigate the differential expression of antioxidant isoenzyme genes in rice plants from the seeds irradiated with low doses (2, 4, 8, and 16 Gy) of gamma-radiation. The gammairradiation slightly increased the transcripts of pCuZn-SOD, while those of Fe-SOD, cAPXb, and CATb decreased. However, no substantial differences were observed in the expression of all the isoenzyme genes between the control and irradiated groups. In this study, gene specific primers for thirteen SOD, APX and CAT isoenzymes were constructed from the full-length cDNAs. The results of RT-PCR analysis obtained by using these primers suggests that the expression levels of SOD, APX, and CAT isoenzyme genes in rice seedlings were hardly affected by gamma-irradiation at the seed stage.

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  6. [국내논문]   Oligomeric Structure of ${\beta}$-Glucosidases  

    Kim, Sang-Yeob (Department of Genetic Engineering, College of Natural Science, Kyungpook National University ) , Kimm, In-Soo (Department of Genetic Engineering, College of Natural Science, Kyungpook National University)
    Journal of photoscience : an international journal officail organ of the korean society of photoscience v.11 no.3 ,pp. 121 - 127 , 2004 , 1225-8555 ,

    초록

    The ${\beta}$ -glucosidases occur widely in all living organisms and has in general a tendency to form oligomers of varying numbers of subunits or aggregates, although the functional implications of such diverse oligomerization schemes remain unclear. In particular, the assembly mode of the oat ${\beta}$ -glucosidase is very unique in that it multimerizes by linear stacking of a hexameric building block to form long fibrillar multimers. Some structural proteins such as actin and tubulin assemble into long fibrils in a helical fashion and several enzymes such as GroEL and Pyrodictium ATPase functional complexes, 20S proteasome of the archaebacterium Thermoplasma acidophilum, and lutamine synthetase fromblue-green algae, assemble into discrete oligomers upto 4 stacked rings to maintain their enzymatic activities. In particular, oat ${\beta}$ -glucosidase exists in vivo as a discrete long fibrillar multimer assembly that is a novel structure for enzyme protein. It is assembled by linear stacking of hollow trimeric units. The fibril has a long central tunnel connecting to the outer medium via regularly distributed side fenestrations. The enzyme active sites are located within the central tunnel and multimerization increases enzyme affinity to the substrates and catalytic efficiency of the enzyme. Although it is suggested that oligomerization may contribute to the enzyme stability and catalytic efficiency of ${\beta}$ -glycosidases, the functional implications of such diverse oligomerization schemes remain unclear so far.

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  7. [국내논문]   Photochemical Cleavage of Dibenzoylmethane and Curcumin in the Presence of N,N-Dimethylaniline in Methanol  

    Kim, Sung-Sik (Department of Chemistry, Chonbuk National University ) , Mah, Yoon-Jung (Department of Chemistry, Chonbuk National University ) , Kim, Ae-Rhan (Department of Chemistry, Chonbuk National University ) , Cho, Kyung-Won (Department of Chemistry, Chonbuk National University)
    Journal of photoscience : an international journal officail organ of the korean society of photoscience v.11 no.3 ,pp. 129 - 132 , 2004 , 1225-8555 ,

    초록

    Photochemically stable dibenzoylmethane and curcumin were cleaved dramatically when they were irradiated in the presence of N,N-dimethylaniline in methanol with 300 nm UV light. Several products such as benzil, secondary product derived from 1,4-diphenyl-1,4-butanedione, and unidentified compound were observed from the photoreactions of dibenzoylmethane with N,N-dimethylaniline. It was also found that one of the primary fragments produced by irradiation of curcumin in methanol were coupled with N,N-dimethylaniline to give a new enone compound, i.e., 1-(4-dimethylaminophenyl)-4-(4-hydroxy-3-methoxyphenyl)-but-3-en-2-one, as the major product.

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  8. [국내논문]   Comparative Dynamics of $tRNA^{val}$ and pBluescript II SK(+) Phagemid Studied with Ethidium Bromide and a Long-lifetime Metal-ligand Complex  

    Kang, Jung-Sook (Department of Oral Biochemistry and Molecular Biology, College of Dentistry, Pusan National University ) , Yoon, Ji-Hye (Department of Oral Biochemistry and Molecular Biology, College of Dentistry, Pusan National University)
    Journal of photoscience : an international journal officail organ of the korean society of photoscience v.11 no.3 ,pp. 133 - 139 , 2004 , 1225-8555 ,

    초록

    The metal-ligand complex, $[Ru(phen)_2(dppz)]^{2+}$ (phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2',3'-c]phenazine) (RuPD), was used as a spectroscopic probe for studying nucleic acid dynamics. The RuPD complex displays a long lifetime and a molecular light switch property upon DNA binding due to shielding of its dppz ligand from water. To show the usefulness of this luminophore (RuPD) for probing nucleic acid dynamics, we compared its intensity and anisotropy decays when intercalated into the $tRNA^{val}$ and pBluescript (pBS) II SK(+) phagemid through a comparison with ethidium bromide (EB), a conventional nucleic acid probe. We used frequency-domain fluorometry with a blue light-emitting diode (LED) as the modulated light source. The mean lifetime for the $tRNA^{val}$ ( TEX>${\tau}$ ${\tau}$ $tRNA^{val}$ (99.9 and 23.6 ns) than for the pBS II SK(+) phagemid (968.7 and 39.5 ns). These results indicate that RuPD can be useful for studying nucleic acid dynamics.

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