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Journal of fisheries science and technology 19건

  1. [국내논문]   Stimulatory Effects of cyclic AMP on Vitellogenin Induction by Estradiol-17$\beta$ in the Primary Culture of Hepatocytes in the Rainbow Trout Oncorhynchus mykiss  

    Yeo In-Kyu (Laboratory of Comparative Physiology, Faculty of Fisheries, Hokkaido University)
    Journal of fisheries science and technology v.1 no.2 ,pp. 153 - 158 , 1998 , 1226-9204 ,

    초록

    Effects of cyclic (c) AMP and G-protein related reagents (3-isobutyl-l-methyxanthine (IBMX), Forskolin (FSK), cholera toxin (CTX), and pertussis toxin (PTX≫ on estradiol-17 $\beta$ induced vitellogenin (VTG) induction were examined in primary hepatocyte cultures in rainbow trout Oncorhynchus mykiss. The addition of IBMX, FSK, or CTX to the incubation medium markedly increased VTG production, while PTX was not effective in stimulating the production. It is well known that cAMP regulates phosphorylation and dephosphorylation through mediation of protein kinase A. These results suggest that VTG production is highly dependent on cAMP state in hepatocytes because of its highly phosphorylated nature.

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  2. [국내논문]   Water and Sediment Characteristics in the Shellfish Farms of the Western Part of Jinhae Bay   피인용횟수: 2

    Choi Hee Gu (Harmful Algal Blooms Research Department, National Fisheries Research & Development Institute ) , Lee Won Chan (Harmful Algal Blooms Research Department, National Fisheries Research & Development Institute ) , Kim Pyoung Joong (Harmful Algal Blooms Research Department, National Fisheries Research & Development Institute ) , Lee Pil Yong (Harmful Algal Blooms Research Department, National Fisheries Research & Development Institute)
    Journal of fisheries science and technology v.1 no.2 ,pp. 159 - 167 , 1998 , 1226-9204 ,

    초록

    The environmental characteristics in shellfish farms were investigated in the western part of Jinhae Bay, 1996. During summer, anoxia and high nutrient concentrations were found in the bottom waters of shellfish farms. The concentrations of particulate organic species in seawaters were enriched, showing an average 57.44 uM for POC, an average 5.45 uM for PON, and an average 0.42uM for PP. The sediments environment in the farms was very polluted. The concentrations of COD and AVS were more than 20 mg/g.dry and 0.5 mg/g.dry, respectively. The total sedimentation rate was high as an average $7.81g/m^2/day$ with organic matter contents of $26\%$ . Oxygen consumption rate was similar to polluted area as an average of $439mg/m^2/day$ . Nutrient release rates were an average of $8.25mg/m^2/day$ for nitrogen and an average of $1.38mg/m^2/day$ for phosphorous. The cluster analysis through environmental data in summer indicated that DO, nutrient in the bottom water, and AVS in the sediment were important factors to characterize the polluted environmental site.

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  3. [국내논문]   On the Circulation in the Jinhae Bay using the Princeton Ocean Model -I. Characteristic in Vertical Tidal Motion-  

    Hong Chul-hoon (Research Center for Ocean Industrial Development, Pukyong National University)
    Journal of fisheries science and technology v.1 no.2 ,pp. 168 - 179 , 1998 , 1226-9204 ,

    초록

    Circulation in the Jinhae Bay in the southern sea of Korea is examined using the Princeton Ocean Model (POM) with a free surface in a sigma coordinate, governed by primitive equations. The model well corresponds to the time series of the observed velocities at several layers obtained from a long-term mooring observation. In the residual velocity field of the model, persistent downward flow fields are formed along the central deep regions in the bay, and they are caused by bottom topographic effect. In addition, a comparison between a depth-averaged (2D) model and the POM is given, and a dependance of the results on bottom drag coefficient is also examined.

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  4. [국내논문]   Dietary Optimum Phosphorus Level of Juvenile Korean Rockfish (Sebastes schlegeli)  

    Lee Sang-Min (Faculty of Marine Bioscience & Technology, Kangnung National University ) , Park Sung-Real (National Fisheries Research and Development Institute ) , Kim Jeong Dae (Dept. of Animal Science, Kangwon National University)
    Journal of fisheries science and technology v.1 no.2 ,pp. 180 - 186 , 1998 , 1226-9204 ,

    초록

    A 10-week feeding experiment was conducted to determine the phosphorus requirement of juvenile Korean rockfish (Sebastes schlegeli). Three replicate groups of fish initially averaging 4.2g were fed the semipurified experimental diets containing graded levels of $NaH_2PO_4\;\cdot\;2H_2O$ to provide from $0.1\%$ to $1.32\%$ total phosphorus level in a flow-through seawater system. Korean rockfish muscle and casein were used as the protein sources of the basal diet. Weight gain, feed efficiency and protein retention of fish fed the $0.35\%$ phosphorus were higher than those of fish fed the $0.1\%$ phosphorus, although no significant improvements $(P>0.01)$ were observed above the level. Determined phosphorus requirement using the broken line model was found to be $0.3\%$ for weight gain. Moisture, protein and lipid contents of whole body and muscle were not affected by dietary phosphorus levels $(P>0.01)$ . Lipid contents of liver in fish fed the $0.1\%$ phosphorus were lower than those in fish fed the $0.35\%$ and $1.32\%$ phosphorus $(P . Dietary phosphorus increased ash and phosphorus contents of the whole body, while those of bone were not affected $(P>0.01)$ . The data obtained in this study indicate that a $0.3\%$ dietaryphosphorus level could be recommended for the optimum growth and efficient nutrient utilization of juvenile Korean rockfish.

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  5. [국내논문]   Application on Microwave Energy in the Preparation of Fish Samples for Electron Microscopic Observation  

    Kim Soo Jin (Department of Microbiology, Pukyong National University ) , Oh Hae Keun (Department of Microbiology, Pukyong National University ) , Song Young-Hwan (Department of Microbiology, Pukyong National University ) , Chung Hyun-Do (Aquatic Life Medicine, Pukyong National University ) , Kim Young-Tae (Department of Microbiology, Pukyong National University ) , Park Nam-Kyu (Biotechnology and Bioengineering Pukyong National University ) , Choi Tae-Jin (Department of Microbiology, Pukyong National University)
    Journal of fisheries science and technology v.1 no.2 ,pp. 187 - 191 , 1998 , 1226-9204 ,

    초록

    Chemotherapy can not be applied for the control of fish viral diseases because viruses depend on host machinery for their replication. Although new control strategies including vaccination are under development, avoidance of virus introduction by rapid and correct diagnosis is the best way of fish viral disease control. Although observation of virus particles with an electron microscope is an easy method for virus detection, it take a few days for the sample preparation. In order to shorten the sample preparation time, microwave radiation was applied in the procedure. With this method, 15 seconds was enough for fixation of virus infected fish samples or cultured cells inoculated with infectious hematopoietic necrosis virus, which takes 2-4 hours with routine methods. Also four minutes was enough for polymerization of embedding resin which takes 24-48 hours with routine methods. Samples prepared with microwave were good enough for direct electron microscopic observation and immunogold labeling assay.

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  6. [국내논문]   Effect of Variable Feed Allowance with Constant Protein Input on Water Quality in Channel Catfish Production Ponds  

    Cho Sung Hwoan (Department of Fisheries and Allied Aquacultures, Auburn University)
    Journal of fisheries science and technology v.1 no.2 ,pp. 192 - 200 , 1998 , 1226-9204 ,

    초록

    This study was carried out to evaluate the effect of feeding higher protein feeds with lesser amount, but feeding the constant total protein input for all treatments, on water quality and nitrite toxicity in channel catfish ponds. There was no significant difference in survival rate among treatments $(P>0.05)$ . Specific growth rate (SGR) for Treatment 1 $(28\%\;protein\;and\;100\%\;of\;satiation)$ was significantly higher $(P>0.05)$ than for Treatment 3 $(36\%\;protein\;and\;87.5\%\;of\;satiation)$ , but not significantly higher than for Treatment 2 $(32\%\;protein\;and\;77.8\%\;of\;satiation)$ at constant digestible energy (DE), 3.08kcal/g (treatments 1, 2 and 3). At constant DE/P (treatments 4, 2 and 5), no significant difference in SGR was observed among treatments. Feed conversion ratio (FCR) slightly improved or improved as dietary protein level increased from $28\%$ to $32\%$ and feed allowance decreased by $12.5\%$ , but did not improve as dietary protein level increased from $32\%$ to $36\%$ and feed allowance decreased by $22.2\%$ , at constant DE and constant DE/P. There was no significant difference in water quality variables, such as total ammonia nitrogen (TAN), nitrite, chlorophyll a, soluble phosphorous concentrations among treatments, but significant difference in water quality variables over time as amount of feed fed increased $(P . There was a trend toward increase in TAN and nitrite over time. A strong linear regression was observed between mean total ammonia nitrogen and nitrite for all treatments Y (Nitrite) = $0.04\times (TAN)+0.01$ , $R_2=0.89$ . Methemoglobin percent in the blood of catifish was not significantly different among treatments. And its mean value was $7.5\%$ , which was relatively low, so that it was not serious problem in catfish production pond under these experiment conditions. There was the stronger linear regression between the percentage of Methemoglobin and the molar ratio of nitrite to chloride rather than nitrite alone: $Y\;(Methemoglobin\;\%)\;=\;58.45\;\times\;(NO^{2-}/Cl^-)\;+\;0.41,\;R^2=0.60$ . These results indicate that deterioration of water quality has no strong impact on poor weight gain for $36\%$ dietary protein in this study.

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  7. [국내논문]   Comparative Biochemical Properties of Proteinases from the Hepatopancreas of Shrimp. -I. Purification of Protease from the Hepatopancreas of Penaeus japonicus-  

    Choi Sung-Mi (Department of Food Science and Nutrition, Yosu National University ) , Oh Eun-Sil (Department of Food Science and Nutrition, Yosu National University ) , Kim Doo-Sang (Department of Food Science and Nutrition, Yosu National University ) , Pyeun Jae-Hyeung (Department of Food and Life Science, Pukyong National University ) , Cho Deuk-Moon (Department of Food and Nutrition, Dong-Pusan University ) , Ahn Chang-Bum (Department of Food Science and Nutrition, Yosu National University ) , Kim Hyeung-Rak (Department of Food Science and Nutrition, Yosu National University)
    Journal of fisheries science and technology v.1 no.2 ,pp. 201 - 208 , 1998 , 1226-9204 ,

    초록

    A protease, which had no tryptic and chymotryptic activity, was purified from the hepatopancreas of shrimp, P. japonicus, through ammonium sulfate fractionation, Q­Sepharose ionic exchange, benzamidine Sepharose 6B affinity, and Sephacryl S-100 gel chromatography. Molecular weight (M.W.) of the protease was estimated to be 24 kDa by gel filtration and showed a single peptide band by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). The protease had a low ratio of acidic to basic amino acids, which is different with pro teases from marine animals. The enzyme was partially inhibited by benzamidine, tosyl-L-lysine chioromethyl ketone (TLCK), phenylmethylsulfonyl fluoride (PMSF), soybean trypsin inhibitor (SBTI), and pepstatin. The enzyme did not have any activity against benzoyl-D,L-arginine p-nitroanilide (BAPNA) or benzoyl-L-tyrosine ethyl ester (BTEE) which is a specific substrate of trypsin and chymotrypsin, respectively. However, the enzyme showed activity forward N-CBZ-L-tyrosine p-nitrophenyl ester (CBZ-Tyr-pNE), N­CBZ-L-tryptophan p-nitrophenyl ester (CBZ-Trp-pNE), and N-CBZ-L-proline p-nitrophenyl ester (CBZ-Pro-pNE). The protease did not showed tryptic and chymotryptic activity, which was not reported in shrimp hepatopancreas.

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  8. [국내논문]   Comparative Biochemical Properties of Proteinases from the Hepatopancreas of Shrimp. -II. Purification of Trypsin from the Hepatopancreas of Penaeus orientalis-  

    Oh Eun-Sil (Department of Food Science and Nutrition, Yosu National University ) , Kim Doo-Sang (Department of Food Science and Nutrition, Yosu National University ) , Jung Kyoo-Jin (Fisheries Research Institute of Chunnam ) , Pyeun Jae-Hyeung (Department of Food and Life Science, Pukyong National University ) , Heu Min-Soo (Department of Food Science, Gyeongsang National University ) , Kim Hyeung-Rak (Department of Food Science and Nutrition, Yosu National University)
    Journal of fisheries science and technology v.1 no.2 ,pp. 209 - 215 , 1998 , 1226-9204 ,

    초록

    Trypsin-like enzyme was purified from shrimp hepatopancreas through Q-Sepharose ionic exchange, benzamidine Sepharose-6B affinity, and Superdex 75 gel chromatography. Purity of trypsin-like enzyme was increased 69-fold with $44\%$ yield. The enzyme consisted of a single polypeptide chain with a molecular weight (M.W.) of 32 kDa judged by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was completely inactivated by serine enzyme inhibitors such as soybean trypsin inhibitor (SBTI), tosyl-L­lysine chloromethyl ketone (TLCK), and leupeptin. However, the enzyme was not affected by tosyl-L-phenylalanine chloromethyl ketone (TPCK) which is a chymotrypsin specific inhibitor. The enzyme had no activity against benzoyl-tyrosine ethyl ester (BTEE) which is a chymotrypsin specific substrate. The enzyme showed high activity on the carboxyl terminal of Phe, Tyr. Glu, Arg, and Asp. However. no activity was detected against the carboxyl terminal of Pro, Trp, Cys, Gly, Val, and Ala.

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  9. [국내논문]   Five species of the Genus Prionospio (Polychaeta: Spionidae) in Kwangyang Bay, Korea  

    Jung Rae-Hong (Department of Oceanography, Inha University ) , Choi Byoung-Mi (Department of Oceanography, Inha University ) , Hong Jae-Sang (Department of Oceanography, Inha University)
    Journal of fisheries science and technology v.1 no.2 ,pp. 216 - 226 , 1998 , 1226-9204 ,

    초록

    Specimens of the spionid polychaetes (Polychaeta: Spionidae) were collected and examined in the subtidal shallow waters of Kwangyang Bay, southern coast of Korea, from April 1990 to November 1994. Five species of the genus Prionospio are described and illustrated: Prionospio (Minuspio) multibranchiata Berkeley, 1927, P. (Prionospio) saccifera Mackie and Hartley, 1990, P. (P.) bocki Soderstrom, 1920, P. (P.) membranacea Imajima, 1990, and P. (P.) paradisea Imajima, 1990. These five species of spionid polychaetes are reported for the first time in Korean waters.

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  10. [국내논문]   Molecular divergence of the fish somatomedins: the single family of insulin­like growth factor (IGF)-I and -II from the teleost, flounder  

    Kim Dong Soo (Department of Microbiology, Pukyong National University ) , Kim Young Tae (Department of Microbiology, Pukyong National University)
    Journal of fisheries science and technology v.1 no.2 ,pp. 227 - 231 , 1998 , 1226-9204 ,

    초록

    The teleosts represent ancient real-bony vertebrates in phylogeny and resemble major genetic patterns to higher vertebrates. In the present study, we have defined the single family of insulin-like growth factors (IGFs) from flounder (Paralichthys olivaceus), compared to the prototype of IGFs observed in the Agnathan hagfish. In flounder, IGFs are clearly diverged into two major types including type I and II, and they are structurally similar by displaying a multidomain structure consisting of five functional regions as previously found in other vertebrates. However, flIGF-I appears to be more basic (pI 8.03) than the flIGF-II (pI 5.34) in the fully processed form for the B to D domain region. The flIGF-I seems to contain an evolutionary conserved Asn-linked glycosylation in E domain, which is not found in flIGF­II. The most interesting feature is that flIGF-II appeared to be structurally close to hagfish IGF in secondary structures, particularly in Band D domains. This could tell us an idea on the molecular divergence of IGFs from the Agnatha to teleosts during the vertebrate phylogeny. It also support, in part, a notion regarding on how IGF-II is appeared as more embryonic during development. Nonetheless, the biologically active B to D domain region of flIGF-II shows significant sequence homology of $65.6\%$ to flIGF-Is and contains the evolutionary conserved insulin-family signature, as well as a reserved recognition site (Lys) in D domain, necessary to generate proteolytic cleavage for E-peptide. A significant structural difference was found in E domain in which flIGF-I possesses two potential alternative splicing donor site at $Val^{17,\;24}$ of E domain. Therefore, it seems so far that IGF-I sorely produces spliced variants due to the spliced E-peptide moiety while IGF-II appears to be maintained in a single type during evolution. IGF-II, however, may be also possible to transcribe unidentified variants, depending on the physiological conditions of tissues in vertebrates in vivo.

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    Fig. 1 이미지

논문관련 이미지