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International journal of oral biology : official j... 5건

  1. [국내논문]   Effects of Achyranthes Radix Extracts on Osteoblasts and Osteocalsts  

    Kim, Kang-Yong (Dept. of Oral Biochemistry, Dankook University ) , Kim, Se-Won (Dept. of Dental Pharmacology, Dankook University ) , Kim, Jung-Keun (Dept. of Oral Biochemistry, Dankook University ) , Ko, Seon-Yle (Dept. of Oral Biochemistry, Dankook University)
    International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute v.30 no.2 ,pp. 39 - 45 , 2005 , 1226-7155 ,

    초록

    The present study was performed to investigate whether Achyranthes Radix extracts play roles in the bone metabolism. Three kinds of Achyranthes Radix extracts (methylene chloride (MC), ethylacetate (Ea), and water (W)) were used for bioassay. We examined cellular activities of osteoblasts by measurement of cell proliferation rate, alkaline phosphatase (ALP) activity, and calcified nodule formation. Osteoclast generation was assayed by measuring the number of tartrate-resistant acid phosphatase (TRAP) (+) multinucleated cells after culture of osteoclast precursor cells. There was a maximum 20% increase in proliferation rate of osteoblastic cells after treatment with MC. First and second subfraction of MC layer increased proliferation of osteoblast. Ea layer and second subfraction of MC layer increased ALP activity. Also MC layer and second subfraction of MC layer from Achyranthes Radix extracts increased the calcified nodule. MC layer and second subfraction of MC layer from Achyranthes Radix extracts significantly decreased in the number of TRAP (+) multinucleated cells. Taken together, Achyranthes Radix stimulates the proliferation and bioactivities of bone-forming osteoblasts, and inhibits activities of bone-resorbing osteoclasts.

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  2. [국내논문]   Effects of Leptin on Osteoclast Generation and Activity  

    Ko, Seon-Yle (Dept. of Oral Biochemistry, Dankook University ) , Cho, Sang-Rae (Dept. of Oral Biochemistry, Dankook University ) , Kim, Se-Won (Dept. of Dental Pharmacology, Dankook University ) , Kim, Jung-Keun (Dept. of Oral Biochemistry, Dankook University)
    International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute v.30 no.2 ,pp. 47 - 57 , 2005 , 1226-7155 ,

    초록

    Leptin, the product of the obese gene, is a circulating hormone secreted primarily from adipocytes. Several results suggest that leptin is important mediators of bone metabolism. The present study was undertaken to determine the effects of leptin on anti-osteoclastogenesis using murine precursors cultured on Ca-P coated plates and on the production of osteoprotegerin (OPG) in osteoblastic cells. Additionally, this study examined the possible involvement of prostaglandin $E_2\;(PGE_2)$ /protein kinase C (PKC)-mediated signals on the effect of leptin on anti-osteoclastogenesis to various culture systems of osteoclast precursors. Osteoclast generation was determined by counting tartrate-resistant acid phosphatase positive [TRAP (+)] multinucleated cells (MNCs). Osteoclastic activity was determined by measuring area of resorption pits formed by osteoclasts on Ca-P coated plate. The number of 1,25-dihydroxycholecalciferol $(1,25[OH]_2D_3)$ - or $PGE_2$ -induced TRAP (+) MNCs in the mouse bone marrow cell culture decreased significantly after treatment with leptin. The number of receptor activator of NF-kB ligand (RANKL)-induced TRAP (+) MNCs in M-CSF dependent bone marrow macrophage (MDBM) cell or RAW264.7 cell culture decreased significantly with leptin treatment. Indomethacin inhibited osteoclast generation induced by $1,25[OH]_2D_3$ and dexamethasone, however, no significant differences were found in the leptin treated group when compared to the corresponding indomethacin group. Phorbol 12-myristate 13-acetate (PMA), a PKC activator, inhibited osteoclast generation induced by $1,25[OH]_2D_3$ . The number of TRAP (+) MNCs decreased significantly with treatment by PMA at concentrations of 0.01 and $0.1{\mu}M$ in culture. Leptin inhibited PMA-mediated osteoclast generation. Isoquinoline-5-sulfonic 2-methyl-1-piperazide dihydrochloride (H7) had no effect on osteoclast generation induced by $1,25[OH]_2D_3$ . Cell culture treatment with leptin resulted in no significant differences in osteoclast generation compared to the corresponding H7 group. Indomethacin showed no significant effect on TRAP (+) MNCs formation from the RAW264.7 cell line. PMA inhibited TRAP (+) MNCs formation induced by RANKL in the RAW264.7 cell culture. H7 had no effect on osteoclast generation from the RAW264.7 cell line. There was no difference compared with the corresponding control group after treatment with leptin. $1,25[OH]_2D_3$ - or $PGE_2$ -induced osteoclastic activity decreased significantly with leptin treatment at a concentration of 100 ng/ml in mouse bone marrow cell culture. Indomethacin, PMA, and H7 significantly inhibited osteoclastic activity induced by $1,25[OH]_2D_3$ in mouse bone marrow cell culture. No significant differences were found between the leptin treated group and the corresponding control group. The secretion of OPG, a substance known to inhibit osteoclast formation, was detected from the osteoblasts. Treatment by leptin resulted in significant increases in OPG secretion by osteoblastic cells. Taken these results, leptin may be an important regulatory cytokines within the bone marrow microenvironment.

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  3. [국내논문]   Postantibiotic Effects and Postantibiotic Sub-MIC Effects of Antibiotics on Fusobacterium nucleatum and Porphyromonas gingivalis   피인용횟수: 1

    Hong, Jin (Department of Oral Microbiology, College of Dentistry, Research Institute of Oral Science, Kangnung National University ) , Lee, Si-Young (Department of Oral Microbiology, College of Dentistry, Research Institute of Oral Science, Kangnung National University)
    International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute v.30 no.2 ,pp. 59 - 63 , 2005 , 1226-7155 ,

    초록

    Postantibiotic effects (PAE) refer to suppression of the bacterial growth following limited periods of exposure to an antibiotic and subsequent to the removal of the antibiotic agent. Fusobacterium nucleatum and Porphyromonas gingivalis are Gram-negative anaerobic bacteria associated with several periodontal diseases. In this study, postantibiotic effects (PAE), postantibiotic sub-MIC effect (PA SME) and sub-MIC effect (SME) of antibiotics on F. nucleatum ATCC 25586 and P. gingivalis W50 were investigated. The PAE was induced by 10X the MIC of antibiotic and antibiotic was eliminated by washing. The PA SMEs were studied by addition of 0.1, 0.2 and 0.3X MICs during the postantibiotic phase of the bacteria, and the SMEs were studied by exposition of the bacteria to antibiotic at the sub-MICs only. Amoxicillin, doxycycline and tetracycline induced PAE for F. nucleatum ATCC 25586 and P. gingivalis W50. But metronidazole and penicillin induced PAE for only F. nucleatum ATCC 25586. Metronidazole and doxycycline induced PA SME and SME for both species of anaerobic bacteria used in this study. The PA SME values for both strains were substantially longer than the SME values. The present study showed the existence of PAE, PA SME and SME for various antibiotics against F. nucleatum ATCC 25586 and P. gingivalis W50.

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  4. [국내논문]   Measurement of Bacterial (Escherichia coil) Concentration by Flow Cytometry   피인용횟수: 1

    Ji, Suk (Department of Oromaxillofacial Infection & Immunity, School of Dentistry and Dental Research Institute, Seoul National University ) , Lee, Jung-Ok (Department of Oromaxillofacial Infection & Immunity, School of Dentistry and Dental Research Institute, Seoul National University ) , Choi, Young-Nim (Department of Oromaxillofacial Infection & Immunity, School of Dentistry and Dental Research Institute, Seoul National University)
    International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute v.30 no.2 ,pp. 65 - 69 , 2005 , 1226-7155 ,

    초록

    Periodontitis is a multi-microbial disease and the comparison of a series of periodontopathogenic and non-periodontopathogenic bacteria in terms of microbe-host interaction may provide clues to understand the microbial etiology of the disease better. When we deal with twenty different bacterial species in a study, the first technical issue is how to measure the accurate concentration and use the same number of bacterial cells. We measured bacterial concentration by enumerating bacteria stained with SYTOX green for constant time using a flow cytometer and compared the results with those obtained by plate counting. Concentrations calculated by two different methods were very close. Therefore, flow cytometric counting allowed the rapid analysis of live/dead bacteria, offering the advantage of turbidity measurement and that of colony counting together.

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  5. [국내논문]   GABAergic Synaptic Input to Mesencephalic Trigeminal Neurons in Rat  

    Ryu, Hyo-Chel (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University ) , Piao, Zheng Gen (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University ) , Choi, Se-Young (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University ) , Lee, Sung-Joong (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University ) , Park, Kyung-Pyo (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University ) , Kim, Joong-Soo (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University ) , Oh, Seog-Bae (Department of Physiology, College of Dentistry and Dental Research Institute, Seoul National University)
    International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute v.30 no.2 ,pp. 71 - 76 , 2005 , 1226-7155 ,

    초록

    The mesencephalic trigeminal nucleus (Mes V) contains cell bodies of primary afferent sensory neurons that relay proprioceptive information from the periodontium and masticatory muscles and function as typical sensory neurons or potentially as integrative interneurons. In the present study, we studied these two potential functions using combined experimental approaches of retrograde labeling and whole cell patch clamp recording. Mes V neurons that presumably originate from periodontal nerve fibers in subsets of Mes V nucleus were identified by retrograde labeling with a fluorescent dye, DiI, which was applied onto inferior alveolar nerve. These cells were elliptical perikarya shaped cells about $40{\mu}m$ in diameter. In these neurons, we measured high voltage-activated calcium channel (HVACC) currents. $GABA_B$ agonist, baclofen, inhibited calcium currents, and the HVACC currents inhibition by baclofen was voltage-dependent, exhibited prepulse facilitation, indicating that it was mediated by $G_i/_G_o$ protein. Taken together, our results demonstrate that Mes V neurons not only have cell bodies originating from periodontium, but also receive synaptic inputs including GABAergic neurons suggesting that Mes V neurons function as both primary sensory neurons and integrative interneurons.

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