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Biotechnology and bioprocess engineering 18건

  1. [국내논문]   Separation of Amino Acids by Simulated Moving Bed Using Competitive Langmuir Isotherm   피인용횟수: 1

    Yang, Yun-Jeong (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University ) , Lee, Chong-Ho (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University ) , Koo, Yoon-Mo (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University)
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 331 - 338 , 2004 , 1226-8372 ,

    초록

    The Separation of two amino acids, phenylalanine and tryptophan, was carried out using laboratory simulated moving bed (SMB) chromatography. The SMB process consisted of four zones, with each zone having 2 columns. The triangle theory was used to obtain the operating conditions for the SMB. The mass transfer coefficients of the two amino acids were obtained from the best-fit values by comparing simulated and experimental pulse data. The competitive adsorption isotherms of the two amino acids were obtained by single and binary frontal analyses, taking into consideration the competition between the two components. A competitive Langmuir isotherm, obtained from single-component frontal chromatography, was used in the first run, and the isotherm from binary frontal chromatography in the second, with the flow rate of zone 1 modified to improve the purity. Compared to the first and second runs, the competitive Langmuir isotherm from the binary frontal chromatography Showed good agreement with the experimental results. Also, adjusting the flow rate in zone 1 increased the purity of the products. The purities of the phenylalanine in the raffinate and the tryptophan in the extract were 99.84 and $99.99\%$ , respectively.

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  2. [국내논문]   Synthesis and Characterization of Fructooligosaccharides Using Levansucrase with a High Concentration of Sucrose  

    Seo Eun-Seong (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University, Department of Material Chemical and Biochemical Engineering, Chonnam National University ) , Lee Jin-Ha (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University, Engineering Research Institute, Chonnam National University ) , Choi Jae-Young (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University, Department of Material Chemical and Biochemical Engineering, Chonnam National University ) , Seo Mi-Young (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University ) , Lee Hee-Sun (Laboratory of Functional Carbohydrate Enzyme and Microbial Genomics, Chonnam National University, Department of Physics, Chonnam National University ) , Chang Seuk-Sang (Pohang Accelerator Laboratory ) , Lee Hyung-Jong (Department of Molecular and Biotechnology, Chonnam National University, Biology Research Center for Industrial Accelerators, Dongshin University ) , Choi Jeong-Sik (Biology Research Center for Indus) , Kim Doman
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 339 - 344 , 2004 , 1226-8372 ,

    초록

    A method for synthesizing branched fructo-oligosaccharides (BFOS) with a high concentration of sucrose ( $1{\~}3$ M) was developed using levansucrase prepared from Leuconortoc mesenteroides B-1355C. The degree of polymerization of oligosaccharides synthesized according to the present method ranged from 2 to over 15. The synthesized BFOS were stable at a pH ranges of 2 to 4 under $120^{\circ}C$ . The percentage of BFOS in the reaction digest was $95.7\%$ (excluding monosaccharides; $4.3\%$ was levan). BFOS reduced the insoluble glucan formation by Streptococcus sobrinus on the surfaces of glass vials or stainless steel wires in the presence of sucrose. They also reduced the growth and acid productions of S, sobrinus. Oligosaccharides can be used as sweeteners for foods such as beverages requiring thermo- and acid-stable properties and 3s potential inhibitors of dental caries.

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  3. [국내논문]   Anammox Bacteria Enrichment in Upflow Anaerobic Sludge Blanket (UASB) Reactor   피인용횟수: 1

    Thuan Tran-Hung (Department of Environmental Engineering and Biotechnology, Myongji University ) , Jahng Deok-Jin (Department of Environmental Engineering and Biotechnology, Myongji University ) , Jung Jin-Young (Environment and Process Technology Division, Korea Institute of Science and Technology (KIST) ) , Kim Dong-Jin (Department of Environmental Systems Engineering, Hallym University ) , Kim Won-Kyoung (Department of Environmental Engineering and Biotechnology, Myongji University ) , Park Young-Joo (Department of Environmental Engineering and Biotechnology, Myongji University ) , Kim Ji-Eun (Department of Environmental Engineering and Biotechnology, Myongji University ) , Ahn Dae-Hee (Department of Environmental Engineering and Biotechnology, Myongji University)
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 345 - 351 , 2004 , 1226-8372 ,

    초록

    We investigated the anaerobic ammonium oxidation (anammox) reaction in a lab-stale upflow anaerobic sludge blanket (UASB) reactor. Our aim was to detect and enrich the organisms responsible for the anammox reaction using a synthetic medium that contained low concentrations of substrates (ammonium and nitrite). The reactor was inoculated with granular sludge collected from a full-scale anaerobic digestor used for treating brewery wastewater The experiment was performed during 260 days under conditions of constant ammonium concentration ( $50\;mg\;NH_4^+-N/L$ ) and different nitrite concentrations ( $50{\~}150\;mg\;NO_2-N/L$ ). After 200 days, anammox activity was observed in the system. The microorganisms involved in this anammox reaction were identified as Candidatus B. Anammoxidans and K. Stuttgartiensis using fluorescence in situ hybridization (FISH ) method.

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  4. [국내논문]   Mass Production of HzSNPV Baculoviruses in Immobilized Heliothis zea (HzAM1) Insect Cell Culture  

    Son Jeong Hwa (Department of Biotechnology & Bioengineering, Pukyong National University ) , Buchholz Rainer (Department of Bioprocess Engineering, Friedrich-Alexander University ) , Kim Sung-Koo (Department of Biotechnology & Bioengineering, Pukyong National University)
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 352 - 355 , 2004 , 1226-8372 ,

    초록

    Heliothis zea (HzAM1) insect cells were immobilized in microspheres by sodium-cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). The highest HzAMl cell density was $7.5{\times}10^7$ cells/mL in the microspheres. After infection of the immobilized cells by Heliothis zea single nuclear polyhedrosis virus (HzSNPV), the highest concentration of HzSNPV (polyhedral inclusion bodies: PIBs) produced was $2.87{\times}10^{10}$ PIBs/mL in the microspheres.

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  5. [국내논문]   Effect of Pleurotus ferulae Extracts on Viability of Human Lung Cancer and Cervical Cancer Cell Lines   피인용횟수: 4

    Choi DuBok (Department of Chemical Engineering, Chosun University ) , Cha Wol-Suk (Department of Chemical Engineering, Chosun University ) , Kang Si-Hyung (Department of Chemical Engineering, Chosun University ) , Lee Byoung-Rai (Department of Biochemistry, College of Medicine, Chosun University)
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 356 - 361 , 2004 , 1226-8372 ,

    초록

    When SiHa cells were incubated for varying periods of time with extracts of PFF and PFM, the cytotoxicity of the ethanol extracts of PFF was higher than those of the other extracts. These results indicated that the extracts from fruiting bodies of p. ferulae contain antitumor Substances. When A549, SiHa and HeLa cells were incubated with different concentrations of PFF and PFM extracts, the ethanol extracts of PFF showed strong cytotoxicity against A549 tells at concentrations over $10{\mu}g/mL$ and against SiHa and HeLa cells at concentrations over $40{\mu}g/mL$ . However, the differences in the cytotoxic effects of the hot water and ethanol extracts of PFM and the hot water extracts of PFF on all 3 cancer cells were not significant. Also, the PFF ethanol extracts induced synergistic effects on the TRAIL-induced apoptosis in A549 cells, which were strongly resistant to TRAIL. These results indicated that ethanol extracts of PFF were the most prominent antitumor agents toward lung cancer cells (A549).

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  6. [국내논문]   Comparing the Performance of One-column Process and Four-zone Simulated Moving Bed by Computer Simulation  

    Kim Young Sik (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University ) , Lee Chong Ho (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University ) , Wankat Phillip C. (School of Chemical Engineering, Purdue University ) , Koon Yoon Mo (Department of Biological Engineering, ERC for Advanced Bioseparation Technology, Inha University)
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 362 - 368 , 2004 , 1226-8372 ,

    초록

    A new one-column chromatography process, analogous to a four-zone simulated moving bed (SMB), was presented. The basic principle of the process was identical to that of a four-zone SMB. The process consisted of one chromatographic column and four tanks, instead of the four columns in the four-zone SMB (1-1-1-1), and has been used for the separation of two amino acids, phenylalanine and tryptophan, using an ion exchange resin. The operating parameters for the one-column process and four-zone SMB were obtained from equilibrium theory. Computer simulations were used to compare the performances of the new one column process to that of the general four-zone SMB, using Aspen $Chromatography^{TM}$ v 11.1. The differences between the one-column and SMB processes in terms of the purities and yields of phenylalanine and tryptophan were less than 4 and about $6\%$ , respectively. The lower purities of the one-column process were due to the loss of the developed concentration profiles in the column when the liquid was stored in tanks. The one-column process gave great flexibility, and would be useful for reconstructing an existing conventional chromatography process to one of a SMB.

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  7. [국내논문]   Optimization of Culture Media for Solid-state Culture of Pleurotus ferulae   피인용횟수: 3

    Cha Wol-Suk (Department of Chemical Engineering, Chosun University ) , Choi DuBok (Department of Chemical Engineering, Chosun University ) , Kang Si-Hyung (Department of Chemical Engineering, Chosun University)
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 369 - 373 , 2004 , 1226-8372 ,

    초록

    In order to elucidate the possibility of artificial production of p. ferulae by solid-state culture, the optimization of culture conditions was carried out. When $NH_4H_2PO_4$ and $CaCO_3$ were used in the cultures using test tube with 30 g of Populus sawdust at $25^{\circ}C{\pm}1$ in the dark, the favored mycelial growth was observed with $1\%$ of $NH_4H_2PO_4$ and the production of polysaccharide was 7.85 mg/100 mg of mycelium with $1\%$ of $CaCO_3$ . The mixtures of $80\%$ of Populus Sawdust and $20\%$ of rice bran at $60\%$ of water content were determined to be optimal for the production of fruiting bodies in the sawdust culture. When three treatments containing various ratios of garlic powder were conducted, yields of fruiting bodies were drasti[ally higher than those of Synthetic mixture without garlic powder The highest yield (143 g/bag) was obtained with $7\%$ garlic powder. The yield of synthetic mixture containing $7\%$ of garlic powder was $83\%$ higher than that of Sawdust culture. The reason why garlic powder did support growth was not clear but it is possible that garlic powder might contain effective components for the formation of fruiting body. The optimal synthetic mixture composition consisted of cotton seed $77\%$ , lime $6.4\%,\;K_2HPO_4\;0.2\%,\;KH_2PO_4\;0.2\%,\;CaHPO_4\;0.2\%$ , corn flour $4\%$ , wheat flour $5\%$ , and garlic pow-der $7\%$ .

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  8. [국내논문]   Optimal conditions for hepatitis B core antigen production in shaked flask fermentation  

    Tey, Beng Ti , Yong, Kok Hoe , Ong, Hong Puay , Ling, Tau Chuan , Ong, Swee Tin , Tan, Yan Peng , Ariff, Arbakariya , Tan, Wen Siang
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 374 - 378 , 2004 , 1226-8372 ,

    초록

    In order to elucidate the possibility of artificial production of p. ferulae by solid-state culture, the optimization of culture conditions was carried out. When $NH_4H_2PO_4$ and $CaCO_3$ were used in the cultures using test tube with 30 g of Populus sawdust at $25^{\circ}C{\pm}1$ in the dark, the favored mycelial growth was observed with $1\%$ of $NH_4H_2PO_4$ and the production of polysaccharide was 7.85 mg/100 mg of mycelium with $1\%$ of $CaCO_3$ . The mixtures of $80\%$ of Populus Sawdust and $20\%$ of rice bran at $60\%$ of water content were determined to be optimal for the production of fruiting bodies in the sawdust culture. When three treatments containing various ratios of garlic powder were conducted, yields of fruiting bodies were drasti[ally higher than those of Synthetic mixture without garlic powder The highest yield (143 g/bag) was obtained with $7\%$ garlic powder. The yield of synthetic mixture containing $7\%$ of garlic powder was $83\%$ higher than that of Sawdust culture. The reason why garlic powder did support growth was not clear but it is possible that garlic powder might contain effective components for the formation of fruiting body. The optimal synthetic mixture composition consisted of cotton seed $77\%$ , lime $6.4\%,\;K_2HPO_4\;0.2\%,\;KH_2PO_4\;0.2\%,\;CaHPO_4\;0.2\%$ , corn flour $4\%$ , wheat flour $5\%$ , and garlic pow-der $7\%$ .

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  9. [국내논문]   Optimal Conditions for Hepatitis B Cove Antigen Production in Shaked Flask Fermentation   피인용횟수: 2

    Tey Beng Ti (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia ) , Yong Kok Hoe (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia ) , Ong Hong Puay (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia ) , Ling Tau Chuan (Department of Process and Food Engineering, Faculty of Engineering, University Putra Malaysia ) , Ong Swee Tin (Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies, University Putra Malaysia ) , Tan Yan Peng (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia ) , Ariff Avbakariya (Department of Biotechnology, Faculty of Food Science and Biotechnology, University Putra Malaysia ) , Tan Wen Siang (Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies, University Putra Malaysia)
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 374 - 378 , 2004 , 1226-8372 ,

    초록

    The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and $40^{\circ}C$ ) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present Study. The highest growth rate is achieved at pH 7, $37^{\circ}C$ and at a rotational Speed of 250 rpm which is 0.927 $h^{-1}$ . The effect of pH on cell growth is more substantial compared to other parameters; it recorded a $123\%$ different between the highest growth rate (0.927 $h^{-1}$ ) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and $40^{\circ}C$ . The yield of protein at pH 7 is $154\%$ higher compared to the lowest yield achieved at pH 5. There is about $28\%$ different of the protein yield for the E. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at $40^{\circ}C$ is $38\%$ higher compared to the lowest yield achieved at $30^{\circ}C$ .

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  10. [국내논문]   Partial Purification and Characterization of an Extracellular Protease from Xenorhabdus nematophilus a Symbiotic Bacterium Isolated from an Entomopathogenic Nematode, Steinernema glaseri   피인용횟수: 1

    Chae Young-Rae (School of Chemical Engineering and Bioengineering, University of Ulsan ) , Ryu Keun-Garp (School of Chemical Engineering and Bioengineering, University of Ulsan)
    Biotechnology and bioprocess engineering v.9 no.5 ,pp. 379 - 382 , 2004 , 1226-8372 ,

    초록

    Entomopathogenic nematodes are used for insect control. Herein, an extracellular protease was partially purified from a culture supernatant of Xenorhabdus nematophilus, a symbiotic bacterium of an entomopathogenic nematode, Steinernema glaseri: using precipitation with $80\%$ v/v isopropyl alcohol followed by gel permeation chromatography with a packed Sephacryl S-300 HR media. The partially purified protease exhibited maximal activity at pH 7 in the presence of 1 mM $CaCl_2$ . The protease was identified as a metallo-protease based on the inhibition of its activity by the metal chelating agent, EDTA.

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