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Biotechnology and bioprocess engineering 20건

  1. [국내논문]   A Simple Purification Procedure of Biologically Active Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) Secreted in Rice Cell Suspension Culture   피인용횟수: 2

    Sharma Niti (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University ) , Park Seung Moon (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University ) , Kwon Tae Ho (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University ) , Kim Dae Hyuk (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University ) , Yang Moon Sik (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 423 - 427 , 2004 , 1226-8372 ,

    초록

    A simple purification procedure of bioactive human granulocyte macrophage colony stimulating factor (hGM-CSF) secreted in rice cell suspension culture has previously been described. In this study the protein was purified to apparent homogeneity with an overall yield of $80.1\%$ by ammonium sulfate precipitation and a single chromatographic step involving FPLCanion exchange chromatography. The purified hGM-CSF revealed at least five glycosylated forms ranging from $21.5{\~}29$ kDa, and its biological activity was independent of the glycosylation pattern. This is the first purification report of recombinant hGM-CSF to apparent homogeneity from rice cell suspension cultures.

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  2. [국내논문]   A Proposal for Optical Diagnostics Through the Enhancement of Diffraction Patterns Using Thin-film Interference Filters  

    Stefanita Carmen Gabriela (University of Alberta, Department of Electrical and Computer Engineering Edmonton ) , Shao Yun Feng (University of Alberta, Department of Electrical and Computer Engineering Edmonton)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 428 - 434 , 2004 , 1226-8372 ,

    초록

    Coarse clumping of solid materials within diseased biological cells can have a marked influence on the light scattering pattern. Perturbations in refractive index lead to distinct varia­tions in the cytometric signature, especially apparent over wide scattering angles. The large dynamic range of scattering intensities restricts collection of data to narrow angular intervals be­lieved to have the highest potential for medical diagnosis. We propose the use of an interfer­ence filter to reduce the dynamic range. Selective attenuation of scattering intensity levels is expected to allow simultaneous data collection over a wide angular interval. The calculated angu­lar transmittance of a commercial shortwave-pass filter of cut-off wavelength 580 nm indicates significant attenuation of scattering peaks below ${\~}\;10^{circ}$ , and reasonable peak equalization at higher angles. For the three-dimensional calculation of laser light scattered by cells we use a spectral method code that models cells as spatially varying dielectrics, stationary in time. How­ever, we perform preliminary experimental testing with the interference filter on polystyrene microspheres instead of biological cells. A microfluidic toolkit is used for the manipulation of the microspheres. The paper intends to illustrate the principle of a light scattering detection system incorporating an interference filter for selective attenuation of scattering peaks.

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  3. [국내논문]   Fermentative Production of White Pepper Using Indigenous Bacterial Isolates  

    Thankamani Vaidyanatha Lyer (Department of Biotechnology, University of Kerala, Kariavattom Campus ) , Giridhar Raghavan Nair (Senior Lecturer, Department of Chemical Engineering, TKM College of Engineering)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 435 - 439 , 2004 , 1226-8372 ,

    초록

    Three Bacillus strains were isolated from soil samples. Morphological and physiologi­cal characterization indicated that the isolated strains were B. mycoides, B. licheniformis and B. brevis. White pepper was produced from black pepper by the fermentative method using the isolates in shake flaks as well as in a large-scale fermenter. Volatile oil and piperine contents of the product were $3.2\%$ (v/w) and $4\%$ (v/w) respectively. The moisture content was $15\%$ . The mi­crobial contamination was less than 10 per 100 g. The product also exhibited excellent storage stability.

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  4. [국내논문]   Bipolar Heterothallism, a Principal Mating System of Cordyceps militaris In Vitro   피인용횟수: 1

    Shrestha Bhushan (Department of Applied Biology, Kangwon National University ) , Kim Ho Kyung (Mushtech Co. Ltd ) , Sung Gi Ho (Department of Botany and Plant Pathology, Oregon State University ) , Spatafora Joseph W. (Department of Botany and Plant Pathology, Oregon State University ) , Sung Jae Mo (Department of Applied Biology, Kangwon National University)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 440 - 446 , 2004 , 1226-8372 ,

    초록

    Interest in in vitro study of entomopathogenic fungi, including Cordyceps species, has been increasing due to their valuable bioactive compounds and biocontrol effects. Among Cordyceps species, in vitro stromata of C militaris has been successfully produced and cultivated for industrial purposes. However, genetic study on in vitro stromata formation of C militaris has not been carried out yet. Here, relationship between mating system and perithecial stromata formation of C militaris is reported. Mating system was determined by observing perithecial stromata formation from mono-ascospore cultures and their pair-wise combinations. Certain combinations of mono-ascospore strains produced perithecial club-shaped stromata, whereas other combinations produced either no stromata or only abnormal non-perithecial stromata. Similarly, mono­ascospore cultures without combination produced either no stromata or only abnormal non­perithecial stromata. Despite obvious heterothallism, self-fertility was occasionally observed in few strains of C militaris. These observations indicated that C militaris behaves as a bipolar het­erothallic fungus and requires two mating compatible strains in order to produce regular club­shaped perithecial stromata, a fundamental requirement for its industrial cultivation.

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  5. [국내논문]   Effects of Modifiers on the Supercritical $CO_{2}$ Extraction of Glycyrrhizin from Licorice and the Morphology of Licorice Tissue after Extraction   피인용횟수: 3

    Kim Hyun Seok (Department of Chemical and Biochemical Engineering, University of Suwon ) , Lee Sang Yun (Department of Chemical and Biochemical Engineering, University of Suwon ) , Kim Byung Yong (Department of Food Science and Technology, Kyunghee University ) , Lee Eun Kyu (Department of Chemical Engineering, Hanyang University ) , Ryu Jong Hoon (Department of Chemical and Biochemical Engineering, University of Suwon ) , Lim Gio Bin (Department of Chemical and Biochemical Engineering, University of Suwon)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 447 - 453 , 2004 , 1226-8372 ,

    초록

    Optimal conditions for the supercritical carbon dioxide $(scCO_{2})$ extraction of glycyr­rhizin from licorice (Glycyrrhiza glabra) were investigated, with an emphasis on the types and levels of modifiers. The morphology of the licorice tissue remaining after the $scCO_{2} $ extraction of glycyrrhizin was examined by scanning electron microscopy, coupled with measurements of ab­solute density. Conventional organic solvent extraction was also carried out for purpose of quantitative comparison. At 50 MPa and $60^{circ}C$ glycyrrhizin could not be extracted with pure $scCO_{2}$ , while a considerable amount of glycyrrhizin was extracted when water was added to $scCO_{2}$ as a modifier. The highest recovery was found to be about $97\%$ when $70\%$ aqueous methanol was added to $scCO_{2}$ at a concentration of $15\%$ . The optimal pressure and temperature for the supercritical fluid extraction of glycyrrhizin were observed to be 30 M Pa and $60^{circ}C$ , respectively. Under these conditions, the percentage recovery of glycyrrhizin attained a maximum value of 102.67\pm$ $1.13\%$ within 60 min. Furthermore, in the case of $scCO_{2}$ modified with $70\%$ aqueous methanol, the licorice tissue obtained after extraction was found to be severely de­graded by excessive swelling, and the absolute density of the licorice residues was observed to be the highest.

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  6. [국내논문]   Predispersed Solvent Extraction of Succinic Acid Aqueous Solution by Colloidal Liquid Aphrons in Column  

    Kim Bong Seock (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology ) , Hong Yeon Ki (Department of Chemical Engineering, Chungju National University ) , Huh Yun Suk (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology ) , Hong Won Hi (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 454 - 458 , 2004 , 1226-8372 ,

    초록

    A study of the POSE (predispersed solvent extraction) for succinic acid by colloidalliq­uid aphrons was conducted. The organic phase contaning TOA (tri-n-octylamine) and 1-octanol permits a selective extraction of succinic acid from its aqueous solution. There was no difference of the extractability of POSE and that of conventional mixer-settler type extraction. Taking into account the no mechanical mixing in POSE, it was concluded that the POSE process is more adaptive than the conventional mixer-settler type extraction process. From mass transfer analysis at the various concentration of TOA in counter-current continuous operation, the concentration of TOA had no influence on the mass transfer coefficient. The loading values in continuous POSE were almost same as those in batch operation.

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  7. [국내논문]   The Improvement of Cephalosporin C Production by Fed-batch Culture of Cephalosporium acremonium M25 Using Rice Oil   피인용횟수: 1

    Kim Jin Hee (Department of Chemical and Biological Engineering, Korea University ) , Lim Jung Soo (Department of Chemical and Biological Engineering, Korea University ) , Kim Seung Wook (Department of Chemical and Biological Engineering, Korea University)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 459 - 464 , 2004 , 1226-8372 ,

    초록

    The objective of this study is to improve cephalosporin C (CPC) production byoptimization of medium and culture conditions. A statistical method was introduced to optimize the main culture medium. The main medium for CPC production was optimized using a statistical method. Glucose and corn steep liquor (CSL) were found to be the most effective factors for CPC production. Glucose and CSL were optimized to 2.84 and $6.68\%$ , respectively. CPC produc­tion was improved $50\%$ by feeding of $5\%$ rice oil at day 3rd and 5th day during the shake flask culture of C acremonium M25. The effect of agitation speeds on CPC production in a 2.5-L bio­reactor was also investigated with fed-batch mode. The maximum cell mass (54.5 g/L) was obtained at 600 rpm. However, the maximum CPC production (0.98 g/L) was obtained at 500 rpm. At this condition, the maximum CPC production was improved about $132\%$ compared to the re­sult with batch flask culture.

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  8. [국내논문]   Optimized Conditions for In Situ Immobilization of Lipase in Aldehyde-silica Packed Columns  

    Seo Woo Yong (Department of Environmental Engineering and Biotechnology, Myongji University ) , Lee Kisay (Department of Environmental Engineering and Biotechnology, Myongji University)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 465 - 470 , 2004 , 1226-8372 ,

    초록

    Optimal conditions for the in situ immobilization of lipase in aldehyde-silica packed columns, via reductive amination, were investigated. A reactant mixture, containing lipase and sodium borohydride (NaCBH), was recirculated through an aldehyde-silica packed column, such that the covalent bonding of the lipase, via amination between the amine group of the enzyme and the aldehyde terminal of the silica, and the reduction of the resulting imine group by NaCBH, could occur inside the bed, in situ. Mobile phase conditions in the ranges of pH $7.0{\~}7.8$ , temperatures between $22{\~}28^{circ}C$ and flow rates from $0.8{\~}1.5\;BV/min$ were found to be optimal for the in situ immobilization, which routinely resulted in an immobilization of more than 70 mg­lipase/g-silica. Also, the optimal ratio and concentration for feed reactants in the in situ immobilization: mass ratio [NaCBH]/[lipase] of 0.3, at NaCBH and lipase concentrations of 0.75 and 2.5 g/L, respectively, were found to display the best immobilization characteristics for concentrations of up to 80 mg-lipase/g-silica, which was more than a 2-fold increase in immobilization compared to that obtained by batch immobilization. For tributyrin hydrolysis, the in situ immobilized lipase displayed lower activity per unit mass of enzyme than the batch-immobilized or free lipase, while allowing more than a $45\%$ increase in lipase activity per unit mass of silica compared to batch immobilization, because the quantity of the immobilization on silica was aug­mented by the in situ immobilization methodology used in this study.

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  9. [국내논문]   Effective Extraction of Oligomeric Proanthocyanidin (OPC) from Wild Grape Seeds   피인용횟수: 1

    Huh Yun Suk (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology ) , Hong Tae Hee (Department of Food & Nutrition, Daejeon Health Science College ) , Hong Won Hi (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 471 - 475 , 2004 , 1226-8372 ,

    초록

    The Oligomeric proanthocyanidin (OPC) in green and black tea, grape seeds, grapes and wine has raised much attention but that OPC in wild grape seed remains to be intensively investigated. This study investigated the total OPC contents and total antioxidant activity of wild grape seeds and developed an efficient extraction process with various temperatures, solvent compositions and times. Also, a chromatography column packed with the Dia-ion HP-20 resin was used for further purification of the ope. The total OPC contents were determined with the Folin-Ciocalteu reagent, and the antioxidant activity using total antioxidant potential (TAP) and 1,1-dipheny|-2picrylhydrazy| (DPPH). The yield of final purified OPC was 1.78 (+)-catechin equivalent (CE) g/100 g, with $IC_{50}$ activities of TAP and DPPH of 31.60 and $15.70\;{\mu}\;g/mL$ . These activi­ties of the final purified OPC were about two times higher than that of the BHA used as a refer­ence sample.

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  10. [국내논문]   Rosin Microparticles as Drug Carriers: Influence of Various Solvents on the Formation of Particles and Sustained-release of Indomethacin  

    Lee Chang Moon (Interdisciplinary Program of Biomedical Engineering, Chonnam National University ) , Lim Seung (Ecobio Inc., Business Incubation Center, Chonnam National University ) , Kim Gwang Yun (Ecobio Inc., Business Incubation Center, Chonnam National University ) , Kim Do Man (School of biological Sciences and Technology and Research Institute for Catalysis, Chonnam National University ) , Kim Dong Woon (Department of Health and Hygiene, Gwang-Yang Health College ) , Lee Hyun Chul (Department of Microbiology, Chonnam National University Medical School ) , Lee Ki Young (Department of Applied Chemical Engineering & The Research Institute for Catalysis, Chonnam National University)
    Biotechnology and bioprocess engineering v.9 no.6 ,pp. 476 - 481 , 2004 , 1226-8372 ,

    초록

    The aim of this study was to formulate a sustained release system for indomethacin (IND) with rosin gum obtained from a pine tree. Rosin microparticles were prepared by a disper­sion and dialysis method without the addition of surfactant. In order to investigate the influence of solvents on the formation of colloidal microparitcles, various solvents like ethanol, DMF, DMAc, and acetone were used. The rosin microparticles containing IND were characterized by X­ray differactometry (XRD) and differential scanning calorimetry (DSC). The morphologies of rosin microparticles observed by scanning electron microscopy (SEM) were spherical. The solvents used to dissolve rosin significantly affected the drug content and drug release rate of IND. The release behaviors of IND from the rosin microparticles were dependent on the drug content and size of the particles. Rosin micorparticles with a higher drug content and of a larger particle size had a slower drug release rate. Also, the IND release rate from the rosin microparticles could be regulated by the rosin content in the microparticles. From these results, rosin microparticles have the potential of being used as a sustained release system of IND.

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