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Metabolic engineering 37건

  1. [해외논문]   C2/ Ed. Board  


    Metabolic engineering v.44 ,pp. IFC , 2017 , 1096-7176 ,

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  2. [해외논문]   C2/ Ed. Board   SCIE


    Metabolic engineering v.44 ,pp. IFC - IFC , 2017 , 1096-7176 ,

    초록

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   Engineering Escherichia coli membrane phospholipid head distribution improves tolerance and production of biorenewables   SCIE

    Tan, Zaigao (Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA ) , Khakbaz, Pouyan (Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, USA ) , Chen, Yingxi (Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA ) , Lombardo, Jeremy (NSF Engineering Research Center for Biorenewable Chemicals, Iowa State University, Ames, IA 50011, USA ) , Yoon, Jong Moon (Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA ) , Shanks, Jacqueline V. (Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA ) , Klauda, Jeffery B. (Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, USA ) , Jarboe, Laura R. (Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA)
    Metabolic engineering v.44 ,pp. 1 - 12 , 2017 , 1096-7176 ,

    초록

    Abstract Economically competitive microbial production of biorenewable fuels and chemicals is often impeded by toxicity of the product to the microbe. Membrane damage is often identified as a major mechanism of this toxicity. Prior efforts to strengthen the microbial membrane by changing the phospholipid distribution have largely focused on the fatty acid tails. Herein, a novel strategy of phospholipid head engineering is demonstrated in Escherichia coli . Specifically, increasing the expression of phosphatidylserine synthase (+ pssA ) was found to significantly increase both the tolerance and production of octanoic acid, a representative membrane-damaging solvent. Tolerance of other industrially-relevant inhibitors, such as furfural, acetate, toluene, ethanol and low pH was also increased. In addition to the increase in the relative abundance of the phosphoethanolamine (PE) head group in the +pssA strain, there were also changes in the fatty acid tail composition, resulting in an increase in average length, percent unsaturation and decreased abundance of cyclic rings. This + pssA strain had significant changes in: membrane integrity, surface potential, electrochemical potential and hydrophobicity; sensitivity to intracellular acidification; and distribution of the phospholipid tails, including an increase in average length and percent unsaturation and decreased abundance of cyclic rings. Molecular dynamics simulations demonstrated that the +PE membrane had increased resistance to penetration of ethanol into the hydrophobic core and also the membrane thickness. Further hybrid models in which only the head group distribution or fatty acid tail distribution was altered showed that the increase in PE content is responsible for the increase in bilayer thickness, but the increased hydrophobic core thickness is due to altered distribution of both the head groups and fatty acid tails. This work demonstrates the importance of consideration of the membrane head groups, as well as a modeling approach, in membrane engineering efforts. Highlights Altering the native phospholipid head distribution impacts E. coli robustness. Increasing the PE head group abundance increases fatty acid tolerance and production. The engineered membrane has increased integrity during fatty acid challenge. Simulation of the engineered membrane shows reduced hydrophobic core penetration. Simulation of hybrid membrane models provide mechanistic insight.

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  4. [해외논문]   Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli   SCIE

    Li, Qingyan (Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China ) , Fan, Feiyu (Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China ) , Gao, Xiang (Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China ) , Yang, Chen (Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China ) , Bi, Changhao (Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China ) , Tang, Jinlei (Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China ) , Liu, Tao (Key Laboratory of Systems Microbial Bi) , Zhang, Xueli
    Metabolic engineering v.44 ,pp. 13 - 21 , 2017 , 1096-7176 ,

    초록

    Abstract The MEP pathway genes were modulated to investigate whether there were new rate-limiting steps and toxic intermediates in this pathway. Activating IspG led to significant decrease of cell growth and β-carotene production. It was found that ispG overexpression led to accumulation of intermediate HMBPP, which seriously interfered with synthesis machinery of nucleotide and protein in Escherichia coli . Activation of the downstream enzyme IspH could solve HMBPP accumulation problem and eliminate the negative effects of ispG overexpression. In addition, intermediate MECPP accumulated in the starting strain, while balanced activation of IspG and IspH could push the carbon flux away from MECPP and led to 73% and 77% increase of β-carotene and lycopene titer respectively. Our work for the first time identified HMBPP to be a cytotoxic intermediate in MEP pathway and demonstrated that balanced activation of IspG and IspH could eliminate accumulation of HMBPP and MECPP and improve isoprenoids production. Highlights Activating IspG led to decreased cell growth and β-carotene production. ispG overexpression led to accumulation of intermediate HMBPP. HMBPP accumulation interfered with nucleotide and protein synthesis. Balanced activation of IspG and IspH eliminated HMBPP and MECPP accumulation. β-carotene titer increased 73% by balanced activation of IspG and IspH.

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  5. [해외논문]   Harnessing the respiration machinery for high-yield production of chemicals in metabolically engineered Lactococcus lactis   SCIE

    Liu, Jianming (National Food Institute, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark ) , Wang, Zhihao (National Food Institute, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark ) , Kandasamy, Vijayalakshmi (National Food Institute, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark ) , Lee, Sang Yup (Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Republic of Korea ) , Solem, Christian (National Food Institute, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark ) , Jensen, Peter Ruhdal (National Food Institute, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark)
    Metabolic engineering v.44 ,pp. 22 - 29 , 2017 , 1096-7176 ,

    초록

    Abstract When modifying the metabolism of living organisms with the aim of achieving biosynthesis of useful compounds, it is essential to ensure that it is possible to achieve overall redox balance. We propose a generalized strategy for this, based on fine-tuning of respiration. The strategy was applied on metabolically engineered Lactococcus lactis strains to optimize the production of acetoin and ( R , R )-2,3-butanediol (R-BDO). In the absence of an external electron acceptor, a surplus of two NADH per acetoin molecule is produced. We found that a fully activated respiration was able to efficiently regenerate NAD + , and a high titer of 371mM (32g/L) of acetoin was obtained with a yield of 82% of the theoretical maximum. Subsequently, we extended the metabolic pathway from acetoin to R-BDO by introducing the butanediol dehydrogenase gene from Bacillus subtilis . Since one mole of NADH is consumed when acetoin is converted into R-BDO per mole, only the excess of NADH needs to be oxidized via respiration. Either by fine-tuning the respiration capacity or by using a dual-phase fermentation approach involving a switch from fully respiratory to non-respiratory conditions, we obtained 361mM (32g/L) R-BDO with a yield of 81% or 365mM (33g/L) with a yield of 82%, respectively. These results demonstrate the great potential in using finely-tuned respiration machineries for bio-production. Highlights Fine-tuning of the reducing power availability via respiration. Respiration capacity modulated through hemin concentration. High titer and yield production of acetoin by harnessing respiration. Production of (R,R)-2,3-butanediol by using a fine-tuned respiration. A dual phase fermentation approach developed for (R,R) 2,3-butanediol biosynthesis.

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  6. [해외논문]   Controlling cell volume for efficient PHB production by Halomonas   SCIE

    Jiang, Xiao-Ran (MOE Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China ) , Yao, Zhi-Hao (MOE Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China ) , Chen, Guo-Qiang (MOE Key Lab of Bioinformatics, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China)
    Metabolic engineering v.44 ,pp. 30 - 37 , 2017 , 1096-7176 ,

    초록

    Abstract Bacterial morphology is decided by cytoskeleton protein MreB and cell division protein FtsZ encoded by essential genes mreB and ftsZ , respectively. Inactivating mreB and ftsZ lead to increasing cell sizes and cell lengths, respectively, yet seriously reduce cell growth ability. Here we develop a temperature-responsible plasmid expression system for compensated expression of relevant gene(s) in mreB or ftsZ disrupted recombinants H. campaniensis LS21, allowing mreB or ftsZ disrupted recombinants to grow normally at 30°C in a bioreactor for 12h so that a certain cell density can be reached, followed by 36h cell size expansions or cell shape elongations at elevated 37°C at which the mreB and ftsZ encoded plasmid pTKmf failed to replicate in the recombinants and thus lost themselves. Finally, 80% PHB yield increase was achieved via controllable morphology manipulated H. campaniensis LS21. It is concluded that controllable expanding cell volumes (widths or lengths) provides more spaces for accumulating more inclusion body polyhydroxybutyrate (PHB) and the resulting cell gravity precipitation benefits the final separation of cells and product during downstream. Highlights 80% PHB yield increase was achieved via controllable morphology manipulated Halomonas . PyrF- based gene deletion method was constructed in Halomonas . Larger spherical cells was achieved via inactivation actin-like protein MreB. Longer filamentous cells was achieved via disruption Z-ring formation protein FtsZ. Cell sizes can be controlled by regulating mreB or ftsZ gene expression.

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  7. [해외논문]   Metabolic engineering of Escherichia coli for the synthesis of the quadripolymer poly(glycolate-co-lactate-co-3-hydroxybutyrate-co-4-hydroxybutyrate) from glucose   SCIE

    Li, Zheng-Jun (Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China ) , Qiao, Kangjian (Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States ) , Che, Xue-Mei (School of Life Sciences, Tsinghua University, Beijing 100084, China ) , Stephanopoulos, Gregory (Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States)
    Metabolic engineering v.44 ,pp. 38 - 44 , 2017 , 1096-7176 ,

    초록

    Abstract Escherichia coli was metabolically engineered to effectively produce a series of biopolymers consisted of four types of monomers including glycolate, lactate, 3-hydroxybutyrate and 4-hydroxybutyrate from glucose as the carbon source. The biosynthetic route of novel quadripolymers was achieved by the overexpression of a range of homologous and heterologous enzymes including isocitrate lyase, isocitrate dehydrogenase kinase/phosphatase, glyoxylate/hydroxypyruvate reductase, propionyl-CoA transferase, β-ketothiolase, acetoacetyl-CoA reductase, succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, CoA transferase and PHA synthase. In shake flask cultures using Luria-Bertani medium supplemented with glucose, the recombinant E. coli reached 7.10g/l cell dry weight with 52.60wt% biopolymer content. In bioreactor study, the final cell dry weight was 19.61g/l, containing 14.29g/l biopolymer. The structure of the produced polymer was chemically characterized by proton NMR analysis. Assessment of thermal and mechanical properties demonstrated that the quadripolymer possessed decreased crystallinity and improved toughness, in comparison to poly-3-hydroxybutyrate homopolymer. This is the first study reporting efficient microbial production of the quadripolymer poly(glycolate- co -lactate- co -3-hydroxybutyrate -co -4-hydroxybutyrate) from glucose. Highlights E. coli was engineered to synthesize novel quadripolymer from glucose. The quadripolymer was produced up to 14.29 g/L in bioreactor cultivation. The elongation at break of the synthesized polymer was found to reach 39%.

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  8. [해외논문]   The importance of sourcing enzymes from non-conventional fungi for metabolic engineering and biomass breakdown   SCIE

    Seppä (Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA ) , lä (Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA ) , , Susanna (Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA ) , Wilken, St. Elmo (Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA ) , Knop, Doriv (Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA) , Solomon, Kevin V. , O'Malley, Michelle A.
    Metabolic engineering v.44 ,pp. 45 - 59 , 2017 , 1096-7176 ,

    초록

    Abstract A wealth of fungal enzymes has been identified from nature, which continue to drive strain engineering and bioprocessing for a range of industries. However, while a number of clades have been investigated, the vast majority of the fungal kingdom remains unexplored for industrial applications. Here, we discuss selected classes of fungal enzymes that are currently in biotechnological use, and explore more basal, non-conventional fungi and their underexploited biomass-degrading mechanisms as promising agents in the transition towards a bio-based society. Of special interest are anaerobic fungi like the Neocallimastigomycota , which were recently found to harbor the largest diversity of biomass-degrading enzymes among the fungal kingdom. Enzymes sourced from these basal fungi have been used to metabolically engineer substrate utilization in yeast, and may offer new paths to lignin breakdown and tunneled biocatalysis. We also contrast classic enzymology approaches with emerging ‘omics’-based tools to decipher function within novel fungal isolates and identify new promising enzymes. Recent developments in genome editing are expected to accelerate discovery and metabolic engineering within these systems, yet are still limited by a lack of high-resolution genomes, gene regulatory regions, and even appropriate culture conditions. Finally, we present new opportunities to harness the biomass-degrading potential of undercharacterized fungi via heterologous expression and engineered microbial consortia. Highlights A wealth of enzymes in industrial use are sourced from fungi. Only a few fungal clades have been explored for metabolic engineering applications. Novel biomass-degrading functions are likely to be found in the basal clades. Anaerobic fungi have the highest diversity of biomass-degrading enzymes across fungi. Modern biotechnology promises to unlock the potential of non-model fungi.

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  9. [해외논문]   Production of a bioactive unnatural ginsenoside by metabolically engineered yeasts based on a new UDP-glycosyltransferase from Bacillus subtilis   SCIE

    Liang, Huichao (Corresponding author.) , Hu, Zongfeng , Zhang, Tingting , Gong, Ting , Chen, Jingjing , Zhu, Ping , Li, Yan , Yang, Jinling
    Metabolic engineering v.44 ,pp. 60 - 69 , 2017 , 1096-7176 ,

    초록

    Abstract Ginsenosides are the main bioactive constituents of Panax species, which are biosynthesized by glycosylation at C3-OH and/or C20-OH of protopanaxadiol (PPD), C6-OH and/or C20-OH of protopanaxatriol (PPT). The C12-glycosylated ginsenosides have scarcely been identified from Panax species. The C12-glycosylated ginsenosides produced from PPD by chemical semi-synthesis have been reported to exhibit higher cytotoxicity than the natural ginsenosides. However, the chemical semi-synthesis approach is not practical due to its complexity and high cost. In our study, a new UDP-glycosyltransferase UGT109A1 was identified from Bacillus subtilis . This enzyme transferred a glucose moiety to C3-OH and C20-OH of dammarenediol-II (DM), C3-OH and C12-OH of PPD and PPT respectively to produce the unnatural ginsenosides 3 β-O -Glc-DM, 3 β ,20 S- Di -O -Glc-DM, 3 β ,12 β -Di- O -Glc-PPD and 3 β ,12 β -Di- O -Glc-PPT. Among these unnatural ginsenosides, 3 β ,12 β- Di -O -Glc-PPT is a new compound which has never been reported before. The anti-cancer activities of these unnatural ginsenosides were evaluated in vitro and in vivo . 3 β ,12 β -Di- O -Glc-PPD exhibited higher anti-lung cancer activity than Rg3, which is the most active natural ginsenoside against lung cancer. Finally, we constructed metabolically engineered yeasts to produce 3 β ,12 β -Di- O -Glc-PPD by introducing the genes encoding B. subtilis UGT109A1, Panax ginseng dammarenediol-II synthase (DS), P. ginseng cytochrome P450-type protopanaxadiol synthase (PPDS) together with Arabidopsis thaliana NADPH-cytochrome P450 reductase (ATR1) into Saccharomyces cerevisiae INVSc1. The yield of 3 β ,12 β -Di- O -Glc-PPD was increased from 6.17mg/L to 9.05mg/L by overexpressing tHMG1. Thus, this study has established an alternative route to produce the unnatural ginsenoside 3 β ,12 β -Di- O -Glc-PPD by synthetic biology strategies, which provides a promising candidate for anti-cancer drug discovery. Highlights A new UDP-glycosyltransferase UGT109A1 was identified from Bacillus subtilis . Several unnatural ginsenosides were produced by UGT109A1 catalysis. 3 β ,12 β -Di- O -Glc-PPD exhibited higher anti-lung cancer activity than Rg3. Engineered yeasts were constructed to produce 3 β ,12 β -Di- O -Glc-PPD.

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  10. [해외논문]   Directing enzyme devolution for biosynthesis of alkanols and 1,n-alkanediols from natural polyhydroxy compounds   SCIE

    Dai, Lu (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China ) , Tao, Fei (State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China ) , Tang, Hongzhi (State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China ) , Guo, Yali (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China ) , Shen, Yaling (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, People's Republic of China ) , Xu, Ping (State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China)
    Metabolic engineering v.44 ,pp. 70 - 80 , 2017 , 1096-7176 ,

    초록

    Abstract Primordial enzymes are proposed to possess broad specificities. Through divergence and evolution, enzymes have been refined to exhibit specificity towards one reaction or substrate, and are thus commonly assumed as “specialists”. However, some enzymes are “generalists” that catalyze a range of substrates and reactions. This property has been defined as enzyme promiscuity and is of great importance for the evolution of new functions. The promiscuities of two enzymes, namely glycerol dehydratase and diol dehydratase, were herein exploited for catalyzing long-chain polyols, including 1,2-butanediol, 1,2,4-butanetriol, erythritol, 1,2-pentanediol, 1,2,5-pentanetriol, and 1,2,6-hexanetriol. The specific activities required for catalyzing these six long-chain polyols were studied via in vitro enzyme assays, and the catalytic efficiencies were increased through protein engineering. The promiscuous functions were subsequently applied in vivo to establish 1,4-butanediol pathways from lignocellulose derived compounds, including xylose and erythritol. In addition, a pathway for 1-pentanol production from 1,2-pentanediol was also constructed. The results suggest that exploiting enzyme promiscuity is promising for exploring new catalysts, which would expand the repertoire of genetic elements available to synthetic biology and may provide a starting point for designing and engineering novel pathways for valuable chemicals. Highlights The promiscuity of GDHt and DDHt for catalyzing long-chain polyols was systematically characterized. Novel pathways for 1,4-BD production from erythritol and 1-pentanol production from 1,2-PD were established. The mutants with enhanced promiscuity may serve as a platform for the production of various alcohols and acids. Exploiting enzyme promiscuity is proposed as a promising strategy that enriches the toolbox for biotechnologists.

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