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Infection, genetics and evolution : journal of mol... 26건

  1. [해외논문]   Editorial Board   SCIE


    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. ii - ii , 2018 , 1567-1348 ,

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  2. [해외논문]   Phylogenetic analysis of porcine circovirus type 3 and porcine circovirus type 2 in China detected by duplex nanoparticle-assisted PCR   SCIE

    Zhang, Lingling (Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China ) , Luo, Yakun (Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China ) , Liang, Lin (Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China ) , Li, Jinxiang (Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China ) , Cui, Shangjin (Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. 1 - 6 , 2018 , 1567-1348 ,

    초록

    Abstract Porcine circovirus 2 causes different clinical syndromes resulting in a significant economic loss in the pork industry and PCV3 infection was mainly distributed in the pig herds exhibiting PDNS and reproductive failure symptoms and was widely prevalent in the swine population in several states of the USA. Subsequently, PCV3 was also detected in the Chinese pig herds in Hubei and Shandong Provinces, etc. In a screening of 265 clinical samples from Beijing, Zhejiang, Guangdong and Hebei in China for PCV2, PCV3 and co-infection of PCV2 and PCV3, 14.2%, 16.6% and 6.8% of the samples tested positive, respectively. Under novel and optimized nano-dPCR reaction conditions, two specific fragments of 528 bp (PCV2) and 251 bp (PCV3) were amplified, respectively. No fragments were amplified when other porcine viruses were used as template. In this study, three PCV2 and three PCV3 isolates were evaluated by phylogenetic and alignment analysis and the results revealed that the PCV2 and PCV3 isolates appear new nucleotide substitutions and PCV3 isolates from China still included branch of porcine circovirus in USA. Highlights A new nano-dPCR method could detect PCV2 and PCV3 specifically. Nano-dPCR could be considered as a tool in PCV2 or PCV3 control and prevention strategies. The sequence analysis indicated that three PCV2 isolates appear new nucleotide substitutions and phylogenetic analysis showed that two PCV3 isolates were identical to American PCV3 strain (GenBank: KX778720).

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  3. [해외논문]   A novel high-resolution multilocus sequence typing of Giardia intestinalis Assemblage A isolates reveals zoonotic transmission, clonal outbreaks and recombination   SCIE

    Ankarklev, Johan (Department of Molecular Biosciences, Stockholm University, SE-10691 Stockholm, Sweden ) , Lebbad, Marianne (Department of Cell and Molecular Biology, BMC, Box 596, Uppsala University, SE-751 24 Uppsala, Sweden ) , Einarsson, Elin (Department of Cell and Molecular Biology, BMC, Box 596, Uppsala University, SE-751 24 Uppsala, Sweden ) , Franzé (Integrated Cardio Metabolic Center, Karolinska Institutet, Novum, Box 285, SE-14157 Huddinge, Stockholm, Sweden ) , n, Oscar (Department of Microbiology, National Veterinary Institute, SE-751 89 Uppsala, Sweden ) , Ahola, Harri (Department of Microbiology, National Veterinary Institute, SE-751 89 Uppsala, Sweden ) , Troell, Karin (Department of Cell and Molecular Biology, BMC, Box 596, Uppsala University, SE-751 24 Uppsala, Sweden) , Svä , rd, Staffan G.
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. 7 - 16 , 2018 , 1567-1348 ,

    초록

    Abstract Molecular epidemiology and genotyping studies of the parasitic protozoan Giardia intestinalis have proven difficult due to multiple factors, such as low discriminatory power in the commonly used genotyping loci, which has hampered molecular analyses of outbreak sources, zoonotic transmission and virulence types. Here we have focused on assemblage A Giardia and developed a high-resolution assemblage-specific multilocus sequence typing (MLST) method. Analyses of sequenced G . intestinalis assemblage A genomes from different sub-assemblages identified a set of six genetic loci with high genetic variability. DNA samples from both humans (n = 44) and animals (n = 18) that harbored Giardia assemblage A infections, were PCR amplified (557–700 bp products) and sequenced at the six novel genetic loci. Bioinformatic analyses showed five to ten-fold higher levels of polymorphic sites than what was previously found among assemblage A samples using the classic genotyping loci. Phylogenetically, a division of two major clusters in assemblage A became apparent, separating samples of human and animal origin. A subset of human samples (n = 9) from a documented Giardia outbreak in a Swedish day-care center, showed full complementarity at nine genetic loci (the six new and the standard BG , TPI and GDH loci), strongly suggesting one source of infection. Furthermore, three samples of human origin displayed MLST profiles that were phylogenetically more closely related to MLST profiles from animal derived samples, suggesting zoonotic transmission. These new genotyping loci enabled us to detect events of recombination between different assemblage A isolates but also between assemblage A and E isolates. In summary, we present a novel and expanded MLST strategy with significantly improved sensitivity for molecular analyses of virulence types, zoonotic potential and source tracking for assemblage A Giardia . Highlights A new high-resolution genotyping method for Giardia assemblage A has been developed. Specific Giardia assemblage AI isolates have zoonotic potential. Giardia parasites undergo intra- and inter-assemblage recombination. Graphical abstract [DISPLAY OMISSION]

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  4. [해외논문]   Prevalence, risk factors and genetic characterization of Toxoplasma gondii in sick pigs and stray cats in Jiangsu Province, eastern China   SCIE

    Hou, Zhao-feng (Corresponding author at: College of Veterinary Medicine, Yangzhou University, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Jiangsu Key Laboratory of Zoonosis, Yangzhou, Jiangsu Province 225000, PR China.) , Su, Shi-jie , Liu, Dan-dan , Wang, Le-le , Jia, Chuan-li , Zhao, Zhen-xing , Ma, Yi-fei , Li, Qiao-qiao , Xu, Jin-jun , Tao, Jian-ping
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. 17 - 25 , 2018 , 1567-1348 ,

    초록

    Abstract Toxoplasma gondii is an obligate intracellular parasitic protozoan with a worldwide distribution. The parasites in edible tissues of pigs and oocysts from cats are the major sources of T. gondii infection in humans. However, there are no data from sick pigs in veterinary clinics or from stray cats in Jiangsu Province , eastern China. In total, biological samples from 141 sick pigs and 64 stray cats were collected from this region. The rate of T. gondii infection in sick pigs was 46.81% using a polymerase chain reaction (PCR), and the overall prevalence of toxoplasmosis in stray cats was 34.38% by PCR and an enzyme-linked immunosorbent assay (ELISA). T. gondii was significantly more prevalent in lungs and heart than in liver and spleen ( P T. gondii infection in these pigs. The DNA samples from 17 sick pigs and seven stray cats, were successfully genotyped by multilocus PCR-restriction fragment length polymorphism (PCR-RFLP) with 10 genetic markers [ SAG1 , SAG2 (5’-3’ SAG2 , alt. SAG2 ), SAG3 , GRA6 , PK1 , c22–8 , c29–2 , BTUB , L358 and Apico]. Six distinct genotypes were found, which were designated ToxoDB PCR-RFLP genotypes #9 (Chinese I), #10 (Type I), #213, and #89, and New 1 and New 2. Chinese I is the most prevalent T. gondii genotype in this region. The two new genotypes (designated New 1 and New 2) are reported and the ToxoDB PCR-RFLP genotype #89 is found for the first time in China. Such information will be useful for the prevention, diagnosis and treatment of porcine toxoplasmosis in Jiangsu Province , eastern China. Highlights This is first report of genotyping of T. gondii from sick pigs in veterinary clinics in Eastern China. A widespread exposure to T. gondii among sick pigs and stray cats. Age and geographic region were the risk factors related to T. gondii infection in sick pigs. This is first report of two new genotypes and ToxoDB #89 of T. gondii in China.

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  5. [해외논문]   Genetic diversity and functional analysis of oipA gene in association with other virulence factors among Helicobacter pylori isolates from Iranian patients with different gastric diseases   SCIE

    Farzi, Nastaran (Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran ) , Yadegar, Abbas (Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran ) , Aghdaei, Hamid Asadzadeh (Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran ) , Yamaoka, Yoshio (Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Oita, Japan ) , Zali, Mohammad Reza (Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran)
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. 26 - 34 , 2018 , 1567-1348 ,

    초록

    Abstract Helicobacter pylori ( H . pylori ) is one of the most genetically diverse bacterial pathogens that persistently colonizes the human gastric epithelium. This remarkable genomic plasticity may act as a driving force for successful adaptation and persistence of the bacteria in the harsh gastric environment. Outer inflammatory protein A (OipA) encoded by oipA gene ( HP0638 / hopH ) is a member of the outer membrane proteins (OMPs) of H . pylori involved in induction of IL-8 secretion and is associated with development of peptic ulcer and gastric cancer. Expression of OipA is regulated by phase variation within a CT dinucleotide repeat motif of the oipA gene. In this study we carried out direct DNA sequence analysis of 53 amplified fragments to investigate the oipA “On/Off” status among Iranian H . pylori isolates from patients with various gastric diseases. The prevalence of cagL , cagA , EPIYA motifs, vacA alleles, babA2 and sabA genotypes as well as cag PAI integrity of the isolates were determined by PCR. Our results demonstrated a high prevalence of strains with functional oipA status (79%) and significant associations were found between functional oipA and cagA ( P = 0.027) and vacA s1m1 ( P = 0.022) genotypes. The vacA s1m2 genotype was also found to be statistically associated with PUD ( P = 0.0001). Interestingly, we showed that H . pylori strains with intact cag PAI co-expressed oipA gene in a significant synergistic relationship ( P oipA status and clinical outcomes ( P > 0.05). In conclusion, our findings denotes great diversity in the number and pattern of CT dinucleotide repeats of oipA among Iranian H . pylori strains. The synergistic link between functional oipA and other important virulence factors is proposed to be critical in the pathogenesis of H . pylori , which needs further studies with a larger number of samples. Highlights High prevalence of H . pylori strains with functional oipA status was identified. The vacA s1m2 genotype was found to be statistically associated with PUD. Significant associations were found between functional oipA and cagA and vacA s1m1. Strains with intact cag PAI co-expressed oipA gene in a significant relationship. Great diversity in the number of CT dinucleotide repeats of oipA were observed.

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  6. [해외논문]   Detection and molecular characterization of enteric viruses in children with acute gastroenteritis in Northern Italy   SCIE

    Biscaro, Valeria (Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia-Spedali Civili, Brescia, Italy ) , Piccinelli, Giorgio (Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia-Spedali Civili, Brescia, Italy ) , Gargiulo, Franco (Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia-Spedali Civili, Brescia, Italy ) , Ianiro, Giovanni (Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy ) , Caruso, Arnaldo (Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia-Spedali Civili, Brescia, Italy ) , Caccuri, Francesca (Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia-Spedali Civili, Brescia, Italy ) , De Francesco, Maria Antonia (Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia-Spedali Civili, Brescia, Italy)
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. 35 - 41 , 2018 , 1567-1348 ,

    초록

    Abstract Enteric viral infections are a major concern for public health, and viral acute gastroenteritis is the principal cause of pediatric morbidity and mortality worldwide, mostly in developing countries. The purpose of this study was to determine the prevalence of different enteric viruses detected in a pediatric population with acute gastroenteritis symptoms, and to characterize the strains detected. Stools were collected from children, aged from 2 months to 15 years old, admitted to one of the main hospitals of Northern Italy, between November 2015 and October 2016. Stools were tested for nine enteric viruses (adenovirus, rotavirus A, norovirus, astrovirus, sapovirus, enterovirus, parechovirus, bocavirus and aichivirus) by molecular methods. Furthermore, rotavirus, norovirus and adenovirus were deeply characterized by nucleotide sequencing and phylogenetic analysis. A total of 151 out of 510 (29.6%) stools analyzed resulted positive for at least one of the enteric virus investigated. The most common virus detected was rotavirus A (53/151, 35.1%), followed by norovirus (39/151, 25.8%), adenovirus (35/151, 23.1%), sapovirus (9/151, 6%), enterovirus (5/151, 3.3%), astrovirus (5/151, 3.3%), parechovirus (4/151, 2.6%) and bocavirus (1/151, 0.6%). Aichi virus was not detected in any sample. Co-infections were detected in 12 out of 510 faecal samples (2.3%). These data improved the knowledge of the enteric viruses circulating in children in Northern Italy. In fact, besides rotavirus, adenovirus and norovirus, several viruses circulated across the whole year in the pediatric population object of this study. The introduction of specific viral diagnosis in our clinical setting will improve patient care by reducing unnecessary use of antibiotics addressing the right etiologic diagnosis. Highlights The prevalence of different enteric viral pathogens isolated in a paediatric population was analysed. Our results improved the knowledge of the enteric viruses circulating in children in Northern Italy. The introduction of specific viral diagnosis in our clinical setting will improve patient care by reducing unnecessary use of antibiotics.

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  7. [해외논문]   Vitamin D pathway genetic variants are able to influence sofosbuvir and its main metabolite pharmacokinetics in HCV mono-infected patients   SCIE

    Cusato, Jessica (Corresponding author at: Laboratory of Clinical Pharmacology and Pharmacogenetics, University of Turin, Department of Medical Sciences, Amedeo di Savoia Hospital, Corso Svizzera 164, 10149 Turin, (Italy).) , De Nicolò , , Amedeo , Boglione, Lucio , Favata, Fabio , Ariaudo, Alessandra , Mornese Pinna, Simone , Carcieri, Chiara , Guido, Federica , Avataneo, Valeria , Cariti, Giuseppe , Di Perri, Giovanni , D'Avolio, Antonio
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. 42 - 47 , 2018 , 1567-1348 ,

    초록

    Abstract Vitamin D levels and genetic variants were associated with drug outcome/toxicity and concentrations. The plasma exposure of GS-331007, the main sofosbuvir metabolite, has been related to SVR. We evaluated the impact of polymorphisms in genes ( CYP27B1 , CYP24A1 , VDBP and VDR ) related to vitamin D pathway on sofosbuvir and GS-331007 plasma levels in HCV mono-infected patients at one month of treatment. Polymorphisms were investigated through real-time PCR; drug plasma quantification was performed through a UHPLC-MS/MS method. GS-331007 levels were associated with CYP24A1rs2248359 and VDRCdx2 variants in all the analyzed patients and linear regression analysis showed that sex, body mass index, HCV genotype, baseline estimated glomerular filtration rate, VDRCdx2AG/GG and CYP27B1-1260TT genotypes significantly predict concentrations. We performed sub-analyses considering the HCV genotype and the concomitant drug, identifying polymorphisms associated with GS-331007 concentrations. This is the first study focusing on vitamin D pathway gene variants and DAAs concentrations, but further studies are required. Highlights Vitamin D is a modulator of biological processes, including immune system. Genetic polymorphisms involved in vitamin D pathway influenced drug concentrations. Vitamin D gene SNPs significantly predict sofosbuvir metabolite concentrations. Pharmacogenetics could be helpful in the prediction of good response patients. Graphical abstract [DISPLAY OMISSION]

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  8. [해외논문]   Infectious bursal disease virus in Algeria: Detection of highly pathogenic reassortant viruses   SCIE

    Abed, Mouna (Algiers High Veterinary School (ENSV), Issad Abbes Street, Oued Smar 16000, Algiers, Algeria ) , Soubies, Sé (Avian and Rabbit Virology Immunology and Parasitology Unit (VIPAC), French Agency for Food, Environmental and Occupational Heath Safety (ANSES), Zoopole –) , bastien (rue des Fusillés BP 53, 22440 Ploufragan, France ) , Courtillon, Cé (Avian and Rabbit Virology Immunology and Parasitology Unit (VIPAC), French Agency for Food, Environmental and Occupational Heath Safety (ANSES), Zoopole –) , line (rue des Fusillés BP 53, 22440 Ploufragan, France ) , Briand, Franç (Avian and Rabbit Virology Immunology and Parasitology Unit (VIPAC), French Agency for Food, Environmental and Occupational Heath Safety (ANSES), Zoopole –) , ois-Xavier (rue des Fusillés BP 53, 22440 Ploufragan, France ) , Allé (Avian and Rabbit Virology Immunology and Parasitology Unit (VIPAC), French Agency for Food, Environmental and Occupational Heath Safety (ANSES), Zoopole –) , e, Chantal (rue des Fusillés BP 53, 22440 Ploufragan, France ) , Amelot, Michel (Experimental Poultry Unit) , De Boisseson, Claire , Lucas, Pierrick , Blanchard, Yannick , Belahouel, Ali , Kara, Redouane , Essalhi, Abdelhalim , Temim, Soraya , Khelef, Djamel , Eterradossi, Nicolas
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. 48 - 57 , 2018 , 1567-1348 ,

    초록

    Abstract Infectious bursal disease (IBD) is an immunosuppressive viral disease, present worldwide, which causes mortality and immunosuppression in young chickens. The causative agent, the Avibirnavirus IBDV, is a non-enveloped virus whose genome consists of two segments (A and B) of double-stranded RNA. Different pathotypes of IBDV exist, ranging from attenuated vaccine strains to very virulent viruses (vvIBDV). In Algeria, despite the prophylactic measures implemented, cases of IBD are still often diagnosed clinically and the current molecular epidemiology of IBDV remains unknown. The presence of the virus and especially of strains genetically close to vvIBDV was confirmed in 2000 by an unpublished OIE report. In this study, nineteen IBDV isolates were collected in Algeria between September 2014 and September 2015 during clinical outbreaks. These isolates were analyzed at the genetic, antigenic and pathogenic levels. Our results reveal a broad genetic and phenotypic diversity of pathogenic IBDV strains in Algeria, with, i) the circulation of viruses with both genome segments related to European vvIBDV, which proved as pathogenic for specific pathogen-free chickens as vvIBDV reference strain, ii) the circulation of viruses closely related - yet with a specific segment B - to European vvIBDV, their pathogenicity being lower than reference vvIBDV, iii) the detection of reassortant viruses whose segment A was related to vvIBDV whereas their segment B did not appear closely related to any reference sequence. Interestingly, the pathogenicity of these potentially reassortant strains was comparable to that of reference vvIBDV. All strains characterized in this study exhibited an antigenicity similar to the cognate reference IBDV strains. These data reveal the continuous genetic evolution of IBDV strains in Algerian poultry through reassortment and acquisition of genetic material of unidentified origin. Continuous surveillance of the situation as well as good vaccination practice associated with appropriate biosecurity measures are necessary for disease control. Highlights Samples collected in 2014–2015 during IBD outbreaks in Algeria were analyzed. Typical and atypical vvIBDV strains, vaccine and reassortant strains were detected. Reassortant strains had a vvIBDV-like segment A and a segment B of uncertain origin. Pathogenicity of reassortant strains was comparable to that of reference vvIBDV. There is continuous genetic evolution of IBDV strains in Algeria.

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  9. [해외논문]   Ribonucleotide reductase as a drug target against drug resistance Mycobacterium leprae: A molecular docking study   SCIE

    Mohanty, Partha Sarathi (Department of Epidemiology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, M. Miyazaki Marg, Tajganj, Agra, India ) , Bansal, Avi Kumar (Department of Epidemiology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, M. Miyazaki Marg, Tajganj, Agra, India ) , Naaz, Farah (Department of Epidemiology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, M. Miyazaki Marg, Tajganj, Agra, India ) , Gupta, Umesh Datta (National JALMA Institute for Leprosy and Other Mycobacterial Diseases, M. Miyazaki Marg, Tajganj, Agra, India ) , Dwivedi, Vivek Dhar (Department of Epidemiology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases, M. Miyazaki Marg, Tajganj, Agra, India ) , Yadava, Umesh (Department of Physics, Deen Dayal Upadhyay Gorakhpur University, Civil Lines, Gorakhpur, India)
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. 58 - 65 , 2018 , 1567-1348 ,

    초록

    Abstract Leprosy is a chronic infection of skin and nerve caused by Mycobacterium leprae . The treatment is based on standard multi drug therapy consisting of dapsone, rifampicin and clofazamine. The use of rifampicin alone or with dapsone led to the emergence of rifampicin-resistant Mycobacterium leprae strains. The emergence of drug-resistant leprosy put a hurdle in the leprosy eradication programme. The present study aimed to predict the molecular model of ribonucleotide reductase (RNR), the enzyme responsible for biosynthesis of nucleotides, to screen new drugs for treatment of drug-resistant leprosy. The study was conducted by retrieving RNR of M. leprae from GenBank. A molecular 3D model of M. leprae was predicted using homology modelling and validated. A total of 325 characters were included in the analysis. The predicted 3D model of RNR showed that the ϕ and φ angles of 251 (96.9%) residues were positioned in the most favoured regions. It was also conferred that 18 α-helices, 6 β turns, 2 γ turns and 48 helix–helix interactions contributed to the predicted 3D structure. Virtual screening of Food and Drug Administration approved drug molecules recovered 1829 drugs of which three molecules, viz., lincomycin, novobiocin and telithromycin, were taken for the docking study. It was observed that the selected drug molecules had a strong affinity towards the modelled protein RNR. This was evident from the binding energy of the drug molecules towards the modelled protein RNR (−6.10, −6.25 and −7.10). Three FDA-approved drugs, viz., lincomycin, novobiocin and telithromycin, could be taken for further clinical studies to find their efficacy against drug resistant leprosy. Highlights The use of rifampicin alone or with dapsone led to the emergence of rifampicin-resistant Mycobacterium leprae strains. Virtual screening of Food and Drug Administration (FDA)-approved drug molecules recovered 1829 drugs of which three molecules, viz., lincomycin, novobiocin and telithromycin, were taken for the docking study. It was observed that the selected drug molecules had a strong affinity towards the modelled protein RNR. This was evident from the binding energy of the drug molecules towards the modelled protein RNR (−6.08, −5.93 and −7.55). Three FDA-approved drugs, viz., lincomycin, novobiocin and telithromycin, could be taken for further clinical studies to find their efficacy against drug resistant leprosy.

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  10. [해외논문]   Identification and characterization of plasmid-mediated quinolone resistance determinants in Enterobacteriaceae isolated from healthy poultry in Brazil   SCIE

    Ferreira, Joseane Cristina (School of Pharmaceutical Sciences of Ribeirão Preto, São Paulo University (USP), Ribeirão Preto, SP 14040-903, Brazil ) , Penha Filho, Rafael Antonio Casarin (School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP 14884-900, Brazil ) , Kuaye, Ana Paula Yorika (School of Pharmaceutical Sciences of Ribeirão Preto, São Paulo University (USP), Ribeirão Preto, SP 14040-903, Brazil ) , Andrade, Leonardo Neves (School of Pharmaceutical Sciences of Ribeirão Preto, São Paulo University (USP), Ribeirão Preto, SP 14040-903, Brazil ) , Berchieri Junior, Angelo (School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP 14884-900, Brazil ) , Darini, Ana Lú (School of Pharmaceutical Sciences of Ribeirão Preto, São Paulo University (USP), Ribeirão Preto, SP 14040-903, Brazil) , cia da Costa
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases v.60 ,pp. 66 - 70 , 2018 , 1567-1348 ,

    초록

    Abstract The expression of plasmid-mediated quinolone resistance (PMQR) genes confers low-level quinolone and fluoroquinolones resistance alone. However, the association to chromosomal resistance mechanisms determines an expressively higher resistance in Enterobacteriaceae . These mechanisms are horizontally disseminated within plasmids and have contributed to the emergence of bacteria with reduced susceptibility or resistant to therapies worldwide. The epidemiological characterization of PMQR dissemination is highly relevant in the scientific and medical context, to investigate the dissemination within enterobacteria, from different populations, including humans and food-producing animals. In the present study, 200 Enterobacteriaceae isolates were harvested from poultry with cloacal swabs and identified as Escherichia coli (90.5%), Escherichia fergusonii (5.5%), Klebsiella oxytoca (2.5%) and Klebsiella pneumoniae (1.5%). Among isolates evaluated, 46 (23%) harboured PMQR genes including qnr B (43/200), qnr S (2/200) and aac(6′)-Ib -cr (1/200). All isolates carrying PMQR genes showed multidrug-resistance phenotype. The 36 E. coli isolates showed 18 different PFGE types. All E. fergusonii isolates showed the same PFGE type. The two Klebsiella oxytoca belonged to two different PFGE types. The phylogenetic groups A, B1, and D were found among the E. coli harboring PMQR genes. Based on the phylogenetic analysis and PFGE, the population structure of E. coli isolates was diverse, even within the same farm. All isolates carrying qnr B and qnr S genes also harboured ColE-like plasmids. The Southern blot hybridization using the S1 -PFGE revealed that the qnr B genes were located on low molecular weight plasmids, smaller than 10Kb. Resistance plasmids were sequenced and showed 100% identity with plasmid pPAB19-3. The association of PMQR genes with mobile genetic elements, such as transferable plasmids, favours the selection and dissemination of (fluoro) quinolones resistant bacteria among food-producing animals, and may play an important role in the current increased prevalence of resistant bacteria in different environments reported worldwide. Highlights Evaluation of antimicrobial resistance in enterobacteria from commercial chickens High prevalence of PMQR determinants in Enterobacteriaceae isolated from healthy chickens Multidrug resistance (MDR) in commensal Enterobacteriaceae carrying PMQR determinants Dissemination of PMQR occurred by ColE-like plasmids Characterization of plasmids carrying qnr B genes

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