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Journal of bacteriology and virology : JBV 20건

  1. [국내논문]   Epidemiology of Shigellosis in Korea   피인용횟수: 2

    Lee, Je-Chul (Department of Microbiology, Kyungpook National University School of Medicine ) , Jeong, Young-Sook (Department of Microbiology, Kyungpook National University School of Medicine ) , Oh, Jae-Young (Department of Microbiology, Kyungpook National University School of Medicine ) , Kang, Hee-Young (Department of Microbiology, Kyungpook National University School of Medicine ) , Kim, Kwang-Hoon (Department of Microbiology, Kyungpook National University School of Medicine ) , Kim, Jung-Min (Department of Microbiology, Kyungpook National University School of Medicine ) , Lee, Yoo-Chul (Department of Microbiology, Kyungpook National University School of Medicine ) , Cho, Dong-Taek (Department of Microbiology, Kyungpook National University School of Medicine ) , Seol, Sung-Yong (Department of Microbiology, Kyungpook National University School of Medicine)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 41 - 49 , 2006 , 1598-2467 ,

    초록

    Shigellosis is an acute diarrheal disease caused by bacteria of the genus Shigella. Following the occurrence of a large outbreak of shigellosis as well as sporadic cases since 1998, shigellosis has been a major health problem in Korea. There have been major changes in epidemiology during the last five decades concerning shigellosis in terms of total incidence of shigellosis, prevalence of certain serogroups, selection of specific clones, and introduction of new Shigella clones. S. dysenteriae was the most prevalent species until the early twentieth century, S. flexneri was the most prevalent until the late 1980s, and S. sonnei has been the most prevalent since 1990. Diverse serotypes of S. dysenteriae (4 serotypes), S. flexneri (8 serotypes), and S. boydii (4 serotypes) were found during the Korean War and many of these Korean endemic Shigella strains circulated in the community until the late 1970s. However, the endemic strains of S. dysenteriae, S. boydii, and S. sonnei disappeared in the late 1980s. A new clone of S. sonnei that was introduced between the late 1980s and the early 1990s was responsible for a large proportion of shigellosis in recent years. S. flexneri serotype 4a was the most frequently found during the Korean War and then the incidence of S. flexneri 2a gradually increased with time. S. flexneri isolates detected from 1991 to 1997 were all serotype 2a. However, the diverse clones of S. flexneri reemerged in Korea since 1999. It has not been determined whether the S. flexneri strains from the 2000s were the descendants of the Korean endemic strains or imported new strains, but the PFGE patterns were different between S. flexneri strains from the 1980s and 2000s. The widespread of new S. sonnei strains and the persistence of S. flexneri strains are responsible for the endemicity of shigellosis in Korea.

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  2. [국내논문]   Stabilizing Microtubular Network Facilitates the Intracellular Growth of Orientia tsutsugamushi  

    Kim, Mee-Kyung (Inha Research Institute for Medical Sciences ) , Kim, Mi-Jeong (Department of Microbiology, Inha University College of Medicine ) , Lim, Byung-Uk (Department of Microbiology, Inha University College of Medicine ) , Kang, Jae-Seung (Department of Microbiology, Inha University College of Medicine)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 51 - 57 , 2006 , 1598-2467 ,

    초록

    Microtubule network provides many intracellular microbes with an efficient way to move within host cells. Orientia tsutsugamushi move from the cell periphery to the microtubule organizing center (MTOC) by dynein-dependent mechanism. In this study, we investigated the role of microtubule on the growth of O. tsutsugamushi. The treatment of infected cells with taxol as well as daunomycin enhanced the bacterial growth in contrast to colchicine. Immunofluorescent (IF) staining of taxol-treated cells exhibited that O. tsutsugamushi clustered tightly near the nucleus with thick bundles of microtubules, whereas dispersed in the cytoplasm in colchicine-treated cells. These results suggest that microtubule network facilitate the growth of O. tsutsugamushi.

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  3. [국내논문]   Identification of Proteins Induced at Hypoxic and Low pH Conditions inMycobacterium tuberculosisH37Rv  

    Lee, Kil-Soo (Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Daejeon 301-747, Korea. ) , Park, Jeong-Kyu (Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Daejeon 301-747, Korea. ) , Lim, Jae-Hyun (Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Daejeon 301-747, Korea. ) , Kim, Su-Young (Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Daejeon 301-747, Korea. ) , Shin, A-Rum (Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Daejeon 301-747, Korea. ) , Yang, Chul-Su (Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Daejeon 301-747, Korea. ) , Oh, Jae-Hee (Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Daejeon 301-747, Korea. ) , Kwon, Yu-Mi (Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jun) , Song, Chang-Hwa , Jo, Eun-Kyeong , Kim, Hwa-Jung
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 59 , 2006 , 1598-2467 ,

    초록

    Microtubule network provides many intracellular microbes with an efficient way to move within host cells. Orientia tsutsugamushi move from the cell periphery to the microtubule organizing center (MTOC) by dynein-dependent mechanism. In this study, we investigated the role of microtubule on the growth of O. tsutsugamushi. The treatment of infected cells with taxol as well as daunomycin enhanced the bacterial growth in contrast to colchicine. Immunofluorescent (IF) staining of taxol-treated cells exhibited that O. tsutsugamushi clustered tightly near the nucleus with thick bundles of microtubules, whereas dispersed in the cytoplasm in colchicine-treated cells. These results suggest that microtubule network facilitate the growth of O. tsutsugamushi.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  4. [국내논문]   저산소 분압과 낮은 산도 조건에서 발현이 증가되는 결핵균 단백질의 동정  

    이길수 (충남대학교 의과대학 미생물학교실 ) , 박정규 (충남대학교 의과대학 미생물학교실 ) , 임재현 (충남대학교 의과대학 미생물학교실 ) , 김수영 (충남대학교 의과대학 미생물학교실 ) , 신아름 (충남대학교 의과대학 미생물학교실 ) , 양철수 (충남대학교 의과대학 미생물학교실 ) , 오재희 (충남대학교 의과대학 미생물학교실 ) , 권유미 (충남대학교 의과대학 미생물학교실 ) , 송창화 (충남대학교 의과대학 미생물학교실 ) , 조은경 (충남대학교 의과대학 미생물학교실 ) , 김화중 (충남대학교 의과대학 미생물학교실)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 59 - 68 , 2006 , 1598-2467 ,

    초록

    Mycobacterium tuberculosis likely reside within a granuloma as a dormant state. An area of necrosis forms at the center of lung granulomas. Within this area, the bacteria are deprived of nutrients and exposed to harsh conditions, including low pH and anoxia. The response of M. tuberculosis to low pH and low oxygen conditions was investigated in both cellular and extracellular proteins by two-dimensional polyacrylamide gel electrophoresis analysis and MALDI-TOF. Several proteins intensively expressed under low pH and/or hypoxic conditions were found. In the culture filtrate, PhoS1 (Rv0934) and ScoB (Rv2503c) were found in significant amounts under both the low oxygen and acidic stress conditions. These results indeed extend our understanding of acidic response as well as hypoxic in M. tuberculosis and provide an important insight into physiology of the latent bacilli.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [국내논문]   Prevalence ofSalmonellaSerovars Isolated from Domestic Residents and Overseas Travelers in Korea, 2004~2005  

    Kim, Shukho (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul, Korea. ) , Kim, Sung Hun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul, Korea. ) , Chun, Sung Guen (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul, Korea. ) , Choi, Eun Sook (Incheon Airport National Quarantine Station, Korea. ) , Lee, Bok-Kwon (Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul, Korea.)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 69 , 2006 , 1598-2467 ,

    초록

    Mycobacterium tuberculosis likely reside within a granuloma as a dormant state. An area of necrosis forms at the center of lung granulomas. Within this area, the bacteria are deprived of nutrients and exposed to harsh conditions, including low pH and anoxia. The response of M. tuberculosis to low pH and low oxygen conditions was investigated in both cellular and extracellular proteins by two-dimensional polyacrylamide gel electrophoresis analysis and MALDI-TOF. Several proteins intensively expressed under low pH and/or hypoxic conditions were found. In the culture filtrate, PhoS1 (Rv0934) and ScoB (Rv2503c) were found in significant amounts under both the low oxygen and acidic stress conditions. These results indeed extend our understanding of acidic response as well as hypoxic in M. tuberculosis and provide an important insight into physiology of the latent bacilli.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [국내논문]   사람에서 분리된 살모넬라의 국내 유행 혈청형과 해외 유입 혈청형, $2004{\sim}2005$   피인용횟수: 1

    김석호 (국립보건연구원 감염병센터 장내세균팀 ) , 김성훈 (국립보건연구원 감염병센터 장내세균팀 ) , 천성균 (국립보건연구원 감염병센터 장내세균팀 ) , 최은숙 (국립인천공항검역소 ) , 살모넬라 감시그룹 (전국 17개 시.도 보건환경연구원, 13개 검역소 ) , 이복권 (국립보건연구원 감염병센터 장내세균팀)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 69 - 72 , 2006 , 1598-2467 ,

    초록

    Salmonella is a communicable enteric pathogen commonly found in foodborne diseases. We showed the prevalence of S. enterica serovars from domestic residents and overseas travelers. In 2004-2005 years, Weltevreden, Anatum, and Tallahassee were predominant Salmonella serovars from overseas travelers, but Enteritidis, Typhimurium, and Typhi were major Salmonella serovars from domestic residents. In order to analyze the trends of prevalence and distribution of the serovars, Salmonella surveillance with an international study is essential for the disease control and prevention.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [국내논문]   Alteration ingyrA andparC Gene Associated with Fluoroquinolone Resistance ofEnterococcusspp. Isolated from Feces of Chicken  

    Cho, Jae-Keun (Daegu Metropolitan City Research Institute of Health & Environment, Daegu, 706-732, Korea. ) , Kim, Ki-Seuk (College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Korea. ) , Lee, Young-Ju (College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Korea. ) , Park, Cheong-Kyu (College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Korea. ) , Kwak, Dong-Mi (College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Korea. ) , Kim, Ae-Ran (National Veterinary Research and Quarantine Service, Anyang, 430-824, Korea. ) , Kang, Min-Su (National Veterinary Research and Quarantine Service, Anyang, 430-824, Korea. ) , Kim, Jong-Wan (National Veterinary Research and Quarantine Service, Anyang, 430-824, Korea. ) , Kim, Byoung-Han (National Veterinary Research and Quarantine Service, Anyang, 430-824, Korea. ) , Ku, Bok-Kyung (National Veterinary Research and Quarantine Service, Anyang, 430-824, Korea.)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 73 , 2006 , 1598-2467 ,

    초록

    Salmonella is a communicable enteric pathogen commonly found in foodborne diseases. We showed the prevalence of S. enterica serovars from domestic residents and overseas travelers. In 2004-2005 years, Weltevreden, Anatum, and Tallahassee were predominant Salmonella serovars from overseas travelers, but Enteritidis, Typhimurium, and Typhi were major Salmonella serovars from domestic residents. In order to analyze the trends of prevalence and distribution of the serovars, Salmonella surveillance with an international study is essential for the disease control and prevention.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [국내논문]   닭 분변유래 Enterococcus spp.의 Fluoroquinolone계 내성관련 gyrA 및 parC Gene의 변이  

    조재근 (대구광역시 보건환경연구원 ) , 김기석 (경북대학교 수의과대학 ) , 박청규 (경북대학교 수의과대학 ) , 곽동미 (경북대학교 수의과대학 ) , 김애란 (국립수의과학검역원 ) , 강민수 (국립수의과학검역원 ) , 김종완 (국립수의과학검역원 ) , 김병한 (국립수의과학검역원 ) , 구복경 (국립수의과학검역원 ) , 이영주 (경북대학교 수의과대학)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 73 - 78 , 2006 , 1598-2467 ,

    초록

    The purpose of this study was to investigate the fluoroquinolone resistance frequency of Enterococcus spp. from normal chicken feces and to analyse mutations of the gyrA and parC gene associated with fluoroquinolone resistance. Among 52 Enterococcus faecalis and 25 E. faecium isolates, 23 (44.2%) E. faecalis and 7 (28.0%) E. faecium were resistant to ciprofloxacin (CIP) by disc diffusion method. Genetic exchange in gyrA and parC gene among 2 CIP intermediate isolates and 15 CIP resistant isolates were found in the amino acid codon of Ser-83 and Asp-87, and Ser-80 and Glu-84, respectively. These mutants contained a change from Ser to Phe, Val, Tyr, Ile, Thr or Pro at codon 83 and from Glu to Gly or Leu at codon 87 in gyrA gene, and a change from Ser to Ile or Thr at codon 80 and from Glu to Asp or Lys at codon 84 in parC gene. The isolates with mutation in gyrA regardless of a mutation in parC showed high resistance ( $MIC{\ge}232{\mu}g/ml$ ) to CIP, enrofloxacin, norfloxacin and ofloxacin. These results suggested that gyrA gene is the primary target for 4 fluoroquinolones resistance in Enterococcus spp.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  9. [국내논문]   Genetic Variation in the NSP4 Gene of Human Rotavirus Isolated in Seoul  

    Cho, Sung-Lim (Department of Microbiology, Chung-Ang University College of Medicine, Seoul 156-756, Korea. ) , Ahn, Jang-Hoon (Department of Microbiology, Chung-Ang University College of Medicine, Seoul 156-756, Korea. ) , Kim, Kijeong (Department of Microbiology, Chung-Ang University College of Medicine, Seoul 156-756, Korea. ) , Chung, Sang-In (Department of Microbiology, Chung-Ang University College of Medicine, Seoul 156-756, Korea. ) , Lim, Inseok (Department of Pediatrics, Chung-Ang University College of Medicine, Seoul 156-756, Korea. ) , Kim, Wonyong (Department of Microbiology, Chung-Ang University College of Medicine, Seoul 156-756, Korea.)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 79 , 2006 , 1598-2467 ,

    초록

    The purpose of this study was to investigate the fluoroquinolone resistance frequency of Enterococcus spp. from normal chicken feces and to analyse mutations of the gyrA and parC gene associated with fluoroquinolone resistance. Among 52 Enterococcus faecalis and 25 E. faecium isolates, 23 (44.2%) E. faecalis and 7 (28.0%) E. faecium were resistant to ciprofloxacin (CIP) by disc diffusion method. Genetic exchange in gyrA and parC gene among 2 CIP intermediate isolates and 15 CIP resistant isolates were found in the amino acid codon of Ser-83 and Asp-87, and Ser-80 and Glu-84, respectively. These mutants contained a change from Ser to Phe, Val, Tyr, Ile, Thr or Pro at codon 83 and from Glu to Gly or Leu at codon 87 in gyrA gene, and a change from Ser to Ile or Thr at codon 80 and from Glu to Asp or Lys at codon 84 in parC gene. The isolates with mutation in gyrA regardless of a mutation in parC showed high resistance ( $MIC{\ge}232{\mu}g/ml$ ) to CIP, enrofloxacin, norfloxacin and ofloxacin. These results suggested that gyrA gene is the primary target for 4 fluoroquinolones resistance in Enterococcus spp.

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  10. [국내논문]   서울지역에서 분리한 사람 로타바이러스 비 구조단백 NSP4의 유전적 변이   피인용횟수: 1

    조성림 (중앙대학교 의과대학 미생물학교실 ) , 안장훈 (중앙대학교 의과대학 미생물학교실 ) , 김기정 (중앙대학교 의과대학 미생물학교실 ) , 정상인 (중앙대학교 의과대학 미생물학교실 ) , 임인석 (중앙대학교 의과대학 소아과학교실 ) , 김원용 (중앙대학교 의과대학 미생물학교실)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 79 - 87 , 2006 , 1598-2467 ,

    초록

    The nonstructural protein 4 (NSP4) of rotavirus encoded by gene 10, plays an important role in rotavirus pathogenicity. In this study, NSP4 gene sequences of human rotaviruses circulating in Seoul, Korea between March 2004 and April 2005 were determined. The nucleotide sequence data indicated that the NSP4 genes of human rotavirus Korean isolates were 750 or 751 bases in length and encoded one open reading frame of 175 amino acids with two glycosylation sites. The NSP4 of Korean isolates exhibited amino acid sequence homologies between 59.4% and 98.9%. The NSP4 of CAU4 and CAU15 showed a high degree of amino acid sequence homologies with NSP4 genotype A viruses, but the NSP4 of CAU5, CAU6, CAU11, CAU14, CAU16 and CAU22 exhibited a high degree of amino acid sequence homologies with NSP4 genotype B viruses. Interestingly, CAU3 and CAU7 showed low degree of amino acid sequence homology with those of currently described NSP4 genotypes A to D and belonged a distinct lineage on the phylogenetic tree. These findings suggests that distinct NSP4 type was circulating among human rotavirus strains in the local community of Seoul and raising intriguing questions regarding possible explanations for new genotype.

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