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Journal of bacteriology and virology : JBV 19건

  1. [국내논문]   Purification of Native Ag85 Complex, 38-kDa and MTB12 Protein Antigens from the Culture Filtrate ofMycobacterium tuberculosis  

    Lee, Ji-Sook (Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea. ) , Paik, Tae-Hyun (Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea. ) , Yoo, Yung-Choon (Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea. ) , Lee, Junglim (Department of Microbiology, College of Medicine, Konyang University, Daejeon 302-718, Korea. ) , Shin, Arum (Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 301-747, Korea. ) , Song, Chang-Hwa (Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 301-747, Korea. ) , Jo, Eun-Kyung (Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 301-747, Korea. ) , Kim, Hwa-Jung (Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 301-747, Korea. ) , Park, Jung-Kyu (Department of Microbiology, College of Medicine, Chungnam National University, Daejeon 301-747, Korea.)
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 211 , 2006 , 1598-2467 ,

    초록

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  2. [국내논문]   결핵균 배양여과액으로부터 Ag85 Complex, 38-kDa 및 MTB12 항원의 분리정제   피인용횟수: 1

    이지숙 (건양대학교 의과대학 미생물학교실 ) , 유영춘 (건양대학교 의과대학 미생물학교실 ) , 이정림 (건양대학교 의과대학 미생물학교실 ) , 신아름 (충남대학교 의과대학 미생물학교실 ) , 송창화 (충남대학교 의과대학 미생물학교실 ) , 조은경 (충남대학교 의과대학 미생물학교실 ) , 김화중 (충남대학교 의과대학 미생물학교실 ) , 박정규 (충남대학교 의과대학 미생물학교실 ) , 백태현 (건양대학교 의과대학 미생물학교실)
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 211 - 220 , 2006 , 1598-2467 ,

    초록

    The purification of immunodominant native protein antigens from the culture filtrates of Mycobacterium tuberculosis is needed for the development of new vaccines and immunodiagnostic reagents against tuberculosis. In the present study, we conducted large scale purification of well-known secreted antigens, Ag85 complex, 38-kDa, and MTB12, from the culture filtrate proteins (CFPs) prepared from M. tuberculosis H37Rv grown as a surface pellicle on synthetic Sauton medium. The protein and antigen concentrations of culture filtrates were sufficiently increased after 6 week of culture. The MTB12 antigen was detected as early as 1 week of culture, and Ag85 complex and 38-kDa antigen were detected after 2 and 3 week of culture, respectively, by immunodiffusion with specific antiserum against 100-fold concentrated culture filtrates. For large-scale purification, the six-week-culture filtrates of M. tuberculosis H37Rv diluted 2.5-fold with 20 mM Tris-HCl, pH 8.3 were subjected to anion-exchange chromatography. The CFPs were eluted with 100 mM NaCl-20 mM Tris-HCl, pH 8.3 and concentrated by ultrafiltration. The concentrated CFPs were fractionated with ammonium sulfate, and followed by hydrophobic interaction chromatography and anion-exchange chromatography (FPLC). Eventually, 10 mg of Ag85 complex, 0.56 mg of 38-kDa, and 1.81 mg of MTB12 antigens were purified from 1 liter of the six-week-culture filtrates of M. tuberculosis H37Rv which contained 307.81 mg of protein of culture filtrate.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [국내논문]   Comparison of Biological and Genetic Characteristics Between Sucrose-Fermenting and Sucrose-NonfermentingVibrio vulnificusIsolates  

    Kim, Shin-Moo (Department of Clinical Laboratory Science, Wonkwang Health Science College, Iksan 570-750, Korea. ) , So, Hyang-Ah (Department of Microbiology, Jellabukdo Instituite of Health & Environmental, Jeonju 561-844, Korea. ) , Song, Kye-Min (Department of Biology, Graduate School, Sunchon National University, Sunchon 540-742, Korea. ) , Lee, Young-Youp (Department of Biology, Graduate School, Jeonju University, Jeonju 560-759, Korea. ) , Lim, Chae-Won (Department of Clinical Laboratory Science, Wonkwang Health Science College, Iksan 570-750, Korea. ) , Lee, Jae-Hyung (Vestibulocochlear Research Center & Department of Miocrobiology, Wonkwang University School of Medicine, Iksan 570-749, Korea. ) , So, Hong-Seob (Vestibulocochlear Research Center & Department of Miocrobiology, Wonkwang University School of Medicine, Iksan 570-749, Korea. ) , Kim, Jin-Kyung (Vestibulocochlear Research Center & Department of Miocrobiology, Wonkwang University School of Medicine, Iksan 570-749, Korea. ) , Park, Rae-kil (Vestibulocochlear Research Center & Department of Miocrobiology, Wonkwang University School of Medicine, Iksan 570-749, Korea. ) , Park, Seok-Don (Dep)
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 221 , 2006 , 1598-2467 ,

    초록

    The purification of immunodominant native protein antigens from the culture filtrates of Mycobacterium tuberculosis is needed for the development of new vaccines and immunodiagnostic reagents against tuberculosis. In the present study, we conducted large scale purification of well-known secreted antigens, Ag85 complex, 38-kDa, and MTB12, from the culture filtrate proteins (CFPs) prepared from M. tuberculosis H37Rv grown as a surface pellicle on synthetic Sauton medium. The protein and antigen concentrations of culture filtrates were sufficiently increased after 6 week of culture. The MTB12 antigen was detected as early as 1 week of culture, and Ag85 complex and 38-kDa antigen were detected after 2 and 3 week of culture, respectively, by immunodiffusion with specific antiserum against 100-fold concentrated culture filtrates. For large-scale purification, the six-week-culture filtrates of M. tuberculosis H37Rv diluted 2.5-fold with 20 mM Tris-HCl, pH 8.3 were subjected to anion-exchange chromatography. The CFPs were eluted with 100 mM NaCl-20 mM Tris-HCl, pH 8.3 and concentrated by ultrafiltration. The concentrated CFPs were fractionated with ammonium sulfate, and followed by hydrophobic interaction chromatography and anion-exchange chromatography (FPLC). Eventually, 10 mg of Ag85 complex, 0.56 mg of 38-kDa, and 1.81 mg of MTB12 antigens were purified from 1 liter of the six-week-culture filtrates of M. tuberculosis H37Rv which contained 307.81 mg of protein of culture filtrate.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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    Fig. 1 이미지
  4. [국내논문]   Sucrose 발효 및 비발효 Vibrio vulnificus 균주의 생물학적 및 유전학적 성상 비교  

    김신무 (원광보건대학 임상병리과 ) , 소향아 (전북보건환경연구원 미생물부 ) , 송계민 (순천대학교 대학원 생물학과 ) , 이영엽 (전주대학교 대학원 생물학과 ) , 임채원 (원광보건대학 임상병리과 ) , 이재형 (원광대학교 의과대학 전정와우 연구센터 ) , 소홍섭 (원광대학교 의과대학 전정와우 연구센터 ) , 김진경 (원광대학교 의과대학 전정와우 연구센터 ) , 박래길 (원광대학교 의과대학 전정와우 연구센터 ) , 박석돈 (원광대학교 의과대학 피부과교실)
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 221 - 228 , 2006 , 1598-2467 ,

    초록

    Twelve strains of V. vulnificus isolated from clinical specimens in $2002{\sim}2004$ in Jeollado province were determined for their biologic groups, serotypes, presence of vvhA (hemolysin/cytolysin) gene, DNA sequence, and PFGE patterns of NotI-restricted genomic DNA. The following results were obtained. All 12 isolates were biogroup 1, and API 20E profiles were: 5146105 for 5 (41.7%) isolates, and 5148125 for 2 isolates with sucrose fermentation. Ten (83.3%) of the 12 isolates was V. vulnificus serotype O4A, and two sucrose-fermenting isolates belonged to serotype O2. Alleles of cytolysin-hemolysin gene were detected in all 12 isolates. The nucleotide sequences of vvhA genes from strains WKHC 212 and WKHC 221 showed $94{\sim}97%$ similarity compared with those from previously reported 7 strains, YJ016, CMCP6, L-180, CDC B3547, IF Vv10, CIP 75.4T and CNRVC 970121. PFGE of NotI-restricted genomic DNA from the 12 isolates showed approximately 48.5 to 873-kb fragments and they were clustered to five (A to E) patterns. Two sucrose-fermenting isolates belonged to pattern D with 95% similarity with each other. Two strains isolated from two different patients had two identical patterns C and D. It is concluded that sucrose-fermenting strains also exist among clinical isolates of V. vulnificus in Korea, and they can be identified by using API 20E system, and by detecting vvhA gene. DNA sequences and PFGE pattern of NotI-restricted genomic DNA suggested that the two sucrose-fermenting isolates belonged to an identical clone, and two strains each isolated from two different patients belonged to two identical clones.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [국내논문]   Development of a Mass Production Method of Platelet Activating Factor Acetylhydrolase  

    Jong, Yong-Hwa (Department of Microbiology, College of Medicine, Yeungnam University, 317-1 Daemyung-5-dong, Nam-gu, Daegu 705-035, Korea. ) , Chang, Hyeun-Wook (College of Pharmacy, Yeungnam University, Gyongsan 712-749, Korea. ) , Lee, Tae-Yoon (Department of Microbiology, College of Medicine, Yeungnam University, 317-1 Daemyung-5-dong, Nam-gu, Daegu 705-035, Korea.)
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 229 , 2006 , 1598-2467 ,

    초록

    Twelve strains of V. vulnificus isolated from clinical specimens in $2002{\sim}2004$ in Jeollado province were determined for their biologic groups, serotypes, presence of vvhA (hemolysin/cytolysin) gene, DNA sequence, and PFGE patterns of NotI-restricted genomic DNA. The following results were obtained. All 12 isolates were biogroup 1, and API 20E profiles were: 5146105 for 5 (41.7%) isolates, and 5148125 for 2 isolates with sucrose fermentation. Ten (83.3%) of the 12 isolates was V. vulnificus serotype O4A, and two sucrose-fermenting isolates belonged to serotype O2. Alleles of cytolysin-hemolysin gene were detected in all 12 isolates. The nucleotide sequences of vvhA genes from strains WKHC 212 and WKHC 221 showed $94{\sim}97%$ similarity compared with those from previously reported 7 strains, YJ016, CMCP6, L-180, CDC B3547, IF Vv10, CIP 75.4T and CNRVC 970121. PFGE of NotI-restricted genomic DNA from the 12 isolates showed approximately 48.5 to 873-kb fragments and they were clustered to five (A to E) patterns. Two sucrose-fermenting isolates belonged to pattern D with 95% similarity with each other. Two strains isolated from two different patients had two identical patterns C and D. It is concluded that sucrose-fermenting strains also exist among clinical isolates of V. vulnificus in Korea, and they can be identified by using API 20E system, and by detecting vvhA gene. DNA sequences and PFGE pattern of NotI-restricted genomic DNA suggested that the two sucrose-fermenting isolates belonged to an identical clone, and two strains each isolated from two different patients belonged to two identical clones.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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    Fig. 1 이미지
  6. [국내논문]   Platelet Activating Factor Acetylhydrolase의 대량 생산법 개발  

    정영화 (영남대학교 의과대학 미생물학교실 ) , 장현욱 (영남대학교 약학대학 ) , 이태윤 (영남대학교 의과대학 미생물학교실)
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 229 - 235 , 2006 , 1598-2467 ,

    초록

    Platelet activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a potent lipid mediator in a variety of physiological events. PAF is also involved in various pathological events including allergy and inflammation. PAF-acetylhydrolase (PAF-AH) hydrolyzes PAF to produce inactive lyso-PAF. Thus, overproduction of PAF-AF will be useful for the therapeutic valuation of the enzyme. In this study, we established an overproduction method of bovine PAF-AH in Escherichia coli system. We used bovine mammary gland for cDNA cloning. The cDNA had two mismatches of amino acid sequences (Thr-247 to Met and Ile-431 to Thr) compared with the previously reported PAF-AH cDNA (bovine spleen, NM_174578). The recombinant PAF-AH of 43 kDa in molecular size reacted with human PAF-AH polyclonal antibody and showed a strong PAF-AH enzyme activity in an in vitro assay system. The recombinant PAF-AH produced by this study can be applied for various experiments including in vivo models to test its protective activity against PAF-related diseases.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [국내논문]   Expression and Packaging of a Human Endogenous Retrovirus-K Genomic DNA Clone  

    Ha-Lee, Young-Mie (Department of Molecular Biology and Microbiology Tufts University School of Medicine)
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 237 - 245 , 2006 , 1598-2467 ,

    초록

    Human contains large number of human endogenous retroviruses (HERVs) in its genome. One of the HERV families, HERV-K, entered human genome most recently and includes many members with full-length intact proviruses. Normally, these proviruses do not express but infrequently they seem to express in cancers or autoimmune disease patients. To investigate expression mechanisms of these endogenous retroviruses, a DNA copy of HERV-K was cloned and its expression was studied. The transfection of the full-length clone into human cell lines did not produce any detectable viral capsid protein, Gag, and the transcription from its own promoter in LTR was extremely poor. The transcription was less than 10 percent compare to the exogenous retrovirus. However, when the Gag coding region was cloned under CMV promoter, Gag could be expressed efficiently and secreted as particles, probably virus like particles. The efficient expression also required a nuclear export signal. The expressed Gag could also package its own genomic RNA. These results indicate that the LTR of HERV-K is normally not active but its genes have a potential to express and possibly produce infectious particles.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [국내논문]   Spectrin Cleavage Induced by LLP-1 Lentivirus Lytic Peptide Domain in the Intracytoplasmic Tail of Human Immunodeficiency Virus Type 1 GP41 in Rat Organotypic Hippocampal Slice Cultures  

    Lee, Jee-Hee (Department of Microbiology, College of Medicine, Division of Molecular Biology and Neuroscience, Medical Research Center, Ewha Womans University ) , Lee, Eun-Ok (Department of Microbiology, College of Medicine, Division of Molecular Biology and Neuroscience, Medical Research Center, Ewha Womans University ) , Chong, Young-Hae (Department of Microbiology, College of Medicine, Division of Molecular Biology and Neuroscience, Medical Research Center, Ewha Womans University)
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 247 - 254 , 2006 , 1598-2467 ,

    초록

    We previously demonstrated that the lentivirus lytic peptide 1 (LLP-1) corresponding to the carboxyl terminus of HIV-1 gp41 induced cell death in human neuronal cells. Present study was conducted to further elucidate the pathogenic mechanisms involved in HIV-1 gp41-induced neurodegeneration in AIDS patients with cognitive deficits. The effect of LLP-1 on activation of calpain-1, a calcium-activated cysteine protease, which has been implicated in neuronal degeneration and death, was monitored by the proteolysis of spectrin in rat organotypic hippocampal slice cultures. Protease specific spectrin breakdown products revealed that LLP-1 generated ${\sim}150/145-kDa$ fragments characteristic of calpain-1 activation in hippocampus undergoing cell death as evidenced by LDH release. This spectrin cleavage pattern was further confirmed by in vitro calpain-1 proteolysis. Futhermore, ca1pectin and MDL28170, inhibitors of calpain activity, blocked ca1pain-1-mediated spectrin cleavage. Spectrin cleavage likely occurred in the absence of overt synaptic loss, as suggested by the preserved levels of synaptophysin. Among pharmacological agents tested, apocynin, NADPH oxidase inhibitor, ameliorated the LLP-1-induced spectrin. Given the role of spectrin essential for synapse stabilization, LLP-1-induced spectrin cleavage as occurs with the activation of calpain-1 may be an important effector in LLP-1-mediated cell injury in hippocampus, which is primarily linked to cognitive dysfunction.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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    Fig. 1 이미지
  9. [국내논문]   Determination of Porcine Rotavirus Serotypes by RT-PCR and RFLP Analysis   피인용횟수: 1

    Min, Hong-Gi (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University ) , Lim, Yong-Hwan (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University ) , Kang, Shien-Young (Research Institute of Veterinary Medicine/College of Veterinary Medicine, Chungbuk National University)
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 255 - 261 , 2006 , 1598-2467 ,

    초록

    G and P tying of group A porcine rotaviruses (PoRV) from field fecal samples were performed using reversetranscriptase polymerization chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) analysis. After amplifying full length VP7 and partial length VP4 genes, restriction endonucleases were used to digest and analyze the cutting pattern of the gene products. After analysis of digests with restriction endonucleases, seven and six RFLP types were observed for VP7 and VP4, respectively. The G typing analysis of 50 fecal samples revealed that 68% (34/50) were G4, which included G4-like (22/50); 22% (11/50) were G5; 6% (3/50) were G4 and G5 mixed types. The P typing analysis of the same fecal samples revealed that 36% (18/50) were P2B, 52% (26/50) were P9, 1 sample (2%) was a mixture ofP2B and P9. Combinations of G and P types, the G4P2B and G4P9 types including G4-like accounted for 26% (13/50) and 32% (16/50), respectively. The G5P2B and G5P9 type also represented 4% (2/50) and 18% (9/50) of the samples. No G3 and G11 or other new P types were identified from the samples tested. Information on the G and P types and G/P combinations in the field fecal samples is useful for developing more effective PoRV vaccines and understanding the epidemiology of PoRV infections in the field.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  10. [국내논문]   Seroepidemiological Characteristics of Haemorrhagic Fever with Renal Syndrome from 1996 to 2005 in Korea  

    Noh, Yoon-Tae (Division of Arboviruses, Center for Immunology and Pathology, National Institute of Health, Center for Disease Control and Prevention, 194, Tongil-lo, Eunpyung-gu, Seoul 122-701, Korea. ) , Cho, Jung-Eun (Division of Arboviruses, Center for Immunology and Pathology, National Institute of Health, Center for Disease Control and Prevention, 194, Tongil-lo, Eunpyung-gu, Seoul 122-701, Korea. ) , Han, Myung Guk (Division of Arboviruses, Center for Immunology and Pathology, National Institute of Health, Center for Disease Control and Prevention, 194, Tongil-lo, Eunpyung-gu, Seoul 122-701, Korea. ) , Lee, Na-Yeon (Division of Arboviruses, Center for Immunology and Pathology, National Institute of Health, Center for Disease Control and Prevention, 194, Tongil-lo, Eunpyung-gu, Seoul 122-701, Korea. ) , Kim, Su-Yeon (Division of Arboviruses, Center for Immunology and Pathology, National Institute of Health, Center for Disease Control and Prevention, 194, Tongil-lo, Eunpyung-gu, Seoul 122-701, Korea. ) , Chu, Chaeshin (Division of Epidemic Intelligence Service, National Institut) , Lee, Ho-Dong , Nam, Jae-Hwan , Park, Keun-Yong , Shin, Young-Hack , Cho, Hae Wol , Song, Hyeon-Je , Ju, Young-Ran
    Journal of bacteriology and virology : JBV v.36 no.4 ,pp. 263 , 2006 , 1598-2467 ,

    초록

    G and P tying of group A porcine rotaviruses (PoRV) from field fecal samples were performed using reversetranscriptase polymerization chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) analysis. After amplifying full length VP7 and partial length VP4 genes, restriction endonucleases were used to digest and analyze the cutting pattern of the gene products. After analysis of digests with restriction endonucleases, seven and six RFLP types were observed for VP7 and VP4, respectively. The G typing analysis of 50 fecal samples revealed that 68% (34/50) were G4, which included G4-like (22/50); 22% (11/50) were G5; 6% (3/50) were G4 and G5 mixed types. The P typing analysis of the same fecal samples revealed that 36% (18/50) were P2B, 52% (26/50) were P9, 1 sample (2%) was a mixture ofP2B and P9. Combinations of G and P types, the G4P2B and G4P9 types including G4-like accounted for 26% (13/50) and 32% (16/50), respectively. The G5P2B and G5P9 type also represented 4% (2/50) and 18% (9/50) of the samples. No G3 and G11 or other new P types were identified from the samples tested. Information on the G and P types and G/P combinations in the field fecal samples is useful for developing more effective PoRV vaccines and understanding the epidemiology of PoRV infections in the field.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지

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