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Journal of chromatography. B, Analytical technolog... 53건

  1. [해외논문]   Editorial Board   SCI SCIE


    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. ii - ii , 2018 , 1570-0232 ,

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  2. [해외논문]   Separation of aflatoxin B1 from synthetic physiological fluids using talc and diatomite: Kinetic and isotherm aspects   SCI SCIE

    Sprynskyy, Myroslav (Corresponding author.) , Krzemień , -Konieczka, Iwona , Gadzała-Kopciuch, Renata , Buszewski, Bogusław
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. 1 - 8 , 2018 , 1570-0232 ,

    초록

    Abstract The objective of the study was to examine adsorption of the aflatoxin B1 from synthetic gastric fluid and synthetic intestinal fluid by talc, raw and calcined diatomite. The kinetic and equilibrium adsorption processes were studied in the batch adsorption experiments applying high performance liquid chromatography for the aflatoxin B1 determination. The kinetic study showed a very fast adsorption of the aflatoxin B1 onto the selected adsorbents from the both physiological fluids with reaching equilibrium within 1–15min. The aflatoxin B1 was almost completely adsorbed in initial linear step of the kinetic process that can be described well by the zero-order kinetics model. The experimental data of the equilibrium adsorption were characterized using the Langmuir and Freundlich isotherm models. The high adsorption effectiveness was found in a range of 90%–100% and 60%–100% for the diatomite samples and the talc respectively at the initial concentrations of the aflatoxin B1 as 31–300ng/mL. The possible mechanisms of the aflatoxin adsorption onto the used mineral adsorbents are also discussed in the work. Highlights The first data on the aflatoxin B1 adsorption onto talc. The kinetics and isotherms of the aflatoxin B1 adsorption on talc and diatomite. The highly effective and fast adsorption of aflatoxin B1 on tested adsorbents.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   Micro-QuEChERS extraction coupled to GC–MS for a fast determination of Bisphenol A in human urine   SCI SCIE

    Correia-Sá (REQUIMTE/LAQV-GRAQ, Instituto Superior de Engenharia, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal ) , , Luí (CINTESIS –) , sa (Center for Research in Health Technologies and Information Systems, Porto, Portugal ) , Norberto, Só (REQUIMTE/LAQV-GRAQ, Instituto Superior de Engenharia, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal ) , nia (CINTESIS –) , Delerue-Matos, Cristina (Center for Research in Health Technologies and Information Systems, Porto, Portugal ) , Calhau, Conceiç (REQUIMTE/LAQV-GRAQ, Instituto Superior de Engenharia, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal) , ã , o , Domingues, Valentina F.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. 9 - 16 , 2018 , 1570-0232 ,

    초록

    Abstract Bisphenol A (BPA) is considered an endocrine disruptor and public concern over BPA exposure has been raised. Several studies have assessed human exposure to this plasticizer, confirming its ubiquitous presence and highlighting children as a public of special concern. A simple, efficient, cheap and green analytical procedure is reported within this paper. This paper reports, for the first time, the development of a modified Micro-QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method coupled to gas chromatography-mass spectrometry (GC–MS) as a new strategy for the efficient extraction and determination of Bisphenol A in human urine samples. Several parameters that are known to influence extraction were optimized. Good linearity was achieved at the studied concentration range (1–50μg/L), with a correlation coefficient (R 2 ) of 0.998. The optimized method proved to be accurate (≥74% recovery), reproducible ( Highlights First Micro-QuEChERS-GC/MS method for the quantification of BPA in Human Urine. Greener and cheaper alternative to traditional extraction methods. The method was specific, precise, accurate and linear from 1 to 50μg/L. The LOD and LOQ were of 0.13 and 0.43μg/L, respectively. The method was applied for BPA determination in childreńs urines.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  4. [해외논문]   Fibroin/dodecanol floating solidification microextraction for the preconcentration of trace levels of flavonoids in complex matrix samples   SCI SCIE

    Chen, Xuan (Corresponding author.) , Li, Jie , Hu, Shuang , Bai, Xiaohong , Zhao, Haodong , Zhang, Yi
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. 17 - 24 , 2018 , 1570-0232 ,

    초록

    Abstract A new fibroin/dodecanol floating solidification microextraction, coupled with high performance liquid chromatography, was developed and applied for enrichment and quantification of the trace flavonoids in traditional Chinese medicine and biological samples. Also, fibroin sensibilization mechanism was described, and influence of sample matrix to enrichment factor was investigated. In this method, a homogeneous fibroin/dodecanol of dispersed solution was employed as microextraction phase to flavonoids (myricetin, quercetin, isorhamnetin, chrysin, kaempferide), the several critical parameters affecting the performance, such as organic extractant, amount of fibroin in organic extractant, volume of extraction phase, dispersant, salt concentration, pH of sample phase, stirring rate, extraction time, and volume of sample phase were tested and optimized. Under the optimized conditions, enrichment factor of flavonoids ranged from 42.4 to 238.1 in different samples, excellent linearities with r 2 ≥ 0.9968 for all analytes were achieved, limits of detection were less than or equal to 5.0ng/mL, average recoveries were 92.5% to 115.0% in different samples. The new procedure is simple, fast, low cost, environmentally friendly and high EF , it can also be applied to the concentration and enrichment of the trace flavonoids in other complex matrixes. Highlights A new fibroin/dodecanol floating solidification microextraction is proposed. New method with HPLC is used for analysis of flavonoids in complex matrix samples. Each fibroin/dodecanol droplet is as a whole solid/liquid extraction system. Fibroin has a sensitizing effect in improving the extraction of dodecanol. The sample matrix has a critical influence on concentration of flavonoids.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Determination of perfluoroalkyl acid isomers in biosolids, biosolids-amended soils and plants using ultra-high performance liquid chromatography tandem mass spectrometry   SCI SCIE

    Zhang, Hongna (State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China ) , Wen, Bei (State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China ) , Wen, Wen (College of Electrical Engineering, Zhejiang University, Hangzhou 310027, China ) , Ma, Yibing (Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China ) , Hu, Xiaoyu (Beijing Center for Disease Prevention and Control, Beijing 100020, China ) , Wu, Yali (State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China ) , Luo, Lei (State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China ) , Zhang, Shuzhen (State Key Laboratory of Environmental Chemistry)
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. 25 - 33 , 2018 , 1570-0232 ,

    초록

    Abstract Isomer-specific analysis of perfluoroalkyl acids (PFAAs) is important to accurately assess their environmental source, fate, and human risks. In this study, a method was developed for the determination of perfluorooctanoic acid (PFOA), perfluorooctane sulfonate (PFOS), and perfluorohexane sulfonate (PFHxS) isomers in biosolids, biosolids-amended soils and plants using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC–MS/MS). The separation efficiencies of two chromatographic columns and extraction capacities of different methods were tested. Compared with the C18 column (ACQUITY UPLC BEH Shield RP18 column), the column with an alkyl perfluorinated C8 stationary phase (Epic FO LB column), in combination with the distinct MS/MS transitions of analytes, allowed better separation of most isomers. The ion-pair extraction method showed more effective matrix separation than that of the alkaline digestion method, with recoveries ranging from 79.6-105% for biosolids, 80.4-116% for soils, and 68.0-114% for plant tissues. The method detection limits ranged from 10 to 55, 3–13, and 8–58pg/g dry weight for biosolids, soil, and plants, respectively. This method was applied successfully to quantify individual isomers in biosolids, biosolids-amended soils and plants. Six PFOA, eight PFOS, and two PFHxS isomers were found in the samples, with linear isomers being the dominant species. Further analysis revealed that the translocation potentials of branched isomers within plants were higher than those of linear isomers. Highlights A method for analysis of PFAA isomers in biosolids, soil, and plant was developed. Epic FO LB column provided efficient separation of PFOA, PFOS, and PFHxS isomers. Distinct MS/MS transitions allowed further separation for PFAA isomers. Ion-pair extraction method showed satisfactory extraction capacities. Translocation of PFAAs in plants was isomer-specific.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [해외논문]   Quantification of fluorescent dyes in organ tissue samples via HPLC analysis   SCI SCIE

    Morsbach, S. (Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128 Germany ) , Garcí (Department of Anesthesiology, Medical Center of the Johannes Gutenberg University, Langenbeckstraße 1, Mainz, 55131 Germany ) , a-Bardon, A. (Department of Anesthesiology, Medical Center of the Johannes Gutenberg University, Langenbeckstraße 1, Mainz, 55131 Germany ) , Kamuf, J. (Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128 Germany ) , Mü (Department of Anesthesiology, Medical Center of the Johannes Gutenberg University, Langenbeckstraße 1, Mainz, 55131 Germany ) , ller, B. (Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128 Germany ) , Beghersa, N. (Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128 Germany) , Mohr, K. , Landfester, K.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. 34 - 39 , 2018 , 1570-0232 ,

    초록

    Abstract The determination of regional blood flow via the accumulation of fluorescent microspheres is a concept regularly used in medical research. Typically, the microbeads get extracted from the tissue of interest and are then quantified by measuring the absorption or fluorescence of the incorporated dyes without further separation from the medium. However, in that case the absorption spectra of different dyes can overlap when used simultaneously, leading to an overestimation of the concentration. Additionally, background absorption from the medium can be problematic. Therefore, a high performance liquid chromatography method for the simultaneous detection of four dyes (orange, crimson, yellow-green and red) incorporated in different microbeads in samples from biological media such as organ tissue (brain, heart and kidneys) was developed. Since for biological samples often a large sample size is required for sufficient statistics, the method was optimized to yield very short run times. With this method it was possible to detect very low concentrations of only one microsphere per gram of organ tissue. By applying this sensitive quantification technique, it was demonstrated that the application of microbeads for perfusion measurements might not be reliable due to different organ distributions in each animal. Highlights Microspheres for blood perfusion measurements in pigs were extracted from brain, heart and kidney tissue samples. Simultaneous quantification of four different microsphere types by extraction and separation of the encapsulated dyes. Short run times of Microsphere concentrations in animals varied significantly, rendering microspheres problematic for perfusion measurements.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [해외논문]   A HILIC-UHPLC–MS/MS untargeted urinary metabonomics combined with quantitative analysis of five polar biomarkers on osteoporosis rats after oral administration of Gushudan   SCI SCIE

    Wu, Xiao (Corresponding author.) , Huang, Yue , Sun, Jinghan , Wen, Yongqing , Qin, Feng , Zhao, Longshan , Xiong, Zhili
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. 40 - 49 , 2018 , 1570-0232 ,

    초록

    Abstract A HILIC-UHPLC–MS/MS untargeted urinary metabonomic method combined with quantitative analysis of five potential polar biomarkers in rat urine was developed and validated, to further understand the anti-osteoporosis effect of Gushudan(GSD) and its mechanism on prednisolone-induced osteoporosis(OP) rats in this study. The metabolites were separated and identified on Waters BEH HILIC (2.1mm×100mm, 1.7μm) column using the Waters ACQUITY™ ultra performance liquid chromatography system (Waters Corporation, Milford, USA) coupled with a Micromass Quattro Micro™ API mass spectrometer (Waters Corp, Milford, MA, USA). Principal component analysis (PCA) was used to identify potential biomarkers. Primary potential polar biomarkers including creatinine, taurine, betaine, hypoxanthine and cytosine, which were related to energy metabolism, lipid metabolism and amino acid metabolism, were found in the untargeted metabonomic research. Moreover, these targeted biomarkers were further separated and quantified in multiple-reaction monitoring (MRM) with positive ionization mode, using tinidazole as internal standard (I.S.). Good linearities (r>0.99) were obtained for all the analytes with the low limit of quantification from 1.00 to 12.8μg/mL. The relative standard deviation (RSD) of the intra-day and inter-day precisions were within 15.0% and the accuracy ranged from −14.3% to 13.5%. The recovery was more than 85.0%. And the validated method was successfully applied to investigate the urine samples of the control group, prednisolone-induced osteoporosis model group and Gushudan-treatment group in rats. Compared to the control group, the level of creatinine, taurine, betaine, hypoxanthine and cytosine in the model group revealed a significant decrease trend (p t -test. This paper provided a better understanding of the therapeutic effect and mechanism of GSD on prednisolone-induced osteoporosis rats. Highlights A HILIC-UHPLC–MS/MS integrated untargeted and targeted metabonomics was developed. Five potential biomarkers associated with osteoporosis were identified. A HILIC-UHPLC–MS/MS method was developed and validated for the determination of five potential biomarkers.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [해외논문]   Efficient and relatively safe emulsification microextraction using a deep eutectic solvent for influential enrichment of trace main anti-depressant drugs from complicated samples   SCI SCIE

    Moghadam, Ahmad Ghoochani (Corresponding author.) , Rajabi, Maryam , Asghari, Alireza
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. 50 - 59 , 2018 , 1570-0232 ,

    초록

    Abstract In this research work, an efficient, facile, prompt, and relatively safe enrichment procedure, named as air agitated-emulsification microextraction based on a low density-deep eutectic solvent (AA-EME-LD-DES), was applied for the first time to separate trace amounts of the drugs escitalopram, desipramine, and imipramine from complex sample solutions. This bio-degradable and cheap choline chloride-based extracting agent was readily prepared by the easy blending process at the ambient temperature, resulting in a eutectic liquid mixture with distinct features. Also the subsequent usage of an effective proceeding of the current microextraction procedure without a vital requirement for a further purification was adopted as another impressive benefit. Investigation of the main parameters influencing the multivariate technique based on the central composite design (CCD) combined with the desirability function (DF) revealed that pH12.0, 200μL of the extraction solvent, 430μL of the emulsifier solvent, and 14 air agitation cycles led to maximum extraction efficiencies with the DF value close to 0.97. Under the optimal experimental conditions, the wide linear dynamic ranges (LDRs) of 10.0–5000, 15.0–8000, and 15.0–6000ngmL −1 for escitalopram, desipramine, and imipramine were accurately obtainable, respectively, with the determination coefficients (R 2 s) higher than 0.98 and the low limits of detection (LODs) of 3.0–4.5ngmL −1 . The percent extraction recoveries and enrichment factors were found to be adequately quantitative in the spans of 42–68% and 25–40, respectively, possessing good relative standard deviations (%RSDs, n=3) in the range of 3.6–5.7%. Finally, accurate analyses at therapeutically low ranges for the human plasma sample and trace levels for the pharmaceutical wastewater sample were successfully obtained, certifying the appropriate pre-concentration and enrichment capabilities of the proposed microextraction approach. Highlights A new version of low-density solvent based emulsification microextraction was introduced for highly effective enrichment of three antidepressant drugs. The influential extraction agent based on choline chloride was easily synthesized via a facile, safe, and mild route without need to further purification step. Accurate analyses at trace levels close to therapeutic/potentially toxic ranges in human plasma were readily feasible. Proper efficiency, notable simplicity, high speed, tolerable safety, and low cost are prominent features of this proposed extraction approach. Optimization of main parameters impacts were efficiently accomplished through multivariate procedures. Graphical abstract [DISPLAY OMISSION]

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  9. [해외논문]   Development and validation of modified QuEChERS method coupled with LC–MS/MS for simultaneous determination of cymiazole, fipronil, coumaphos, fluvalinate, amitraz, and its metabolite in various types of honey and royal jelly   SCI SCIE

    Zheng, Weijia (Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea ) , Park, Jin-A. (Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea ) , Abd El-Aty, A.M. (Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea ) , Kim, Seong-Kwan (Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea ) , Cho, Sang-Hyun (Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea ) , Choi, Jeong-min (Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea ) , Yi, Hee (Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea ) , Cho, Soo-Min (Department of Veteri) , Ramadan, Amer , Jeong, Ji Hoon , Shim, Jae-Han , Shin, Ho-Chul
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. 60 - 69 , 2018 , 1570-0232 ,

    초록

    Abstract Over the past few decades, honey products have been polluted by different contaminants, such as pesticides, which are widely applied in agriculture. In this work, a modified EN – quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method was developed for the simultaneous quantification of pesticide residues, including cymiazole, fipronil, coumaphos, fluvalinate, amitraz, and its metabolite 2,4-dimethylaniline (2,4-DMA), in four types of honey (acacia, wild, chestnut, and manuka) and royal jelly. Samples were buffered with 0.2M dibasic sodium phosphate (pH 9), and subsequently, acetonitrile was employed as the extraction solvent. A combination of primary secondary amine (PSA) and C18 sorbents was used for purification prior to liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI + /MS-MS) analysis. The estimated linearity measured at six concentration levels presented good correlation coefficients ( R 2 )≥0.99. The recovery, calculated from three different spiking levels, was 62.06–108.79% in honey and 67.58–106.34% in royal jelly, with an RSD Highlights Six pesticide residues in four types of honey and royal jelly were determined by LC–MS/MS analysis. Samples were extracted by a modified EN-QuEChERS method using both C18 sorbent and PSA. Recovery was good when the honey samples were extracted with 0.2M dibasic sodium phosphate buffer and acetonitrile. Three of the market samples tested positive for cymiazole residues.

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  10. [해외논문]   Determination of tropane alkaloids by heart cutting reversed phase – Strong cation exchange two dimensional liquid chromatography   SCI SCIE

    Long, Zhen (Thermofisher Scientific Corporation, Beijing 100080, China ) , Zhang, Yanhai (Thermofisher Scientific Corporation, Beijing 100080, China ) , Gamache, Paul (ThermoFisher Scientific Corporation, Chelmsford 01824, USA ) , Guo, Zhimou (Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China ) , Steiner, Frank (Thermofisher Scientific Corporation, Germering 82110, Germany ) , Du, Nana (Thermofisher Scientific Corporation, Germering 82110, Germany ) , Liu, Xiaoda (Thermofisher Scientific Corporation, Beijing 100080, China ) , Jin, Yan (Thermofisher Scientific Corporation, Beijing 100080, China ) , Liu, Xingguo (Thermofisher Scientific Corporation, Beijing 100080, China ) , Liu, Lvye (Thermofisher Scientific Corporation, Beijing 100080, China)
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1072 ,pp. 70 - 77 , 2018 , 1570-0232 ,

    초록

    Abstract Current Chinese Pharmacopoeia (ChP) standards apply liquid extraction combined with one dimensional liquid chromatography (1DLC) method for determining alkaloids in herbal medicines. The complex pretreatments lead to a low analytical efficiency and possible component loss. In this study, a heart cutting reversed phase – strong cation exchange two dimensional liquid chromatography (RP – SCX 2DLC) approach was optimized for simultaneously quantifying tropane alkaloids (anisodine, scopolamine and hyoscyamine) in herbal medicines and herbal medicine tablets without further treatment of the filtered extract. The chromatographic conditions were systematically optimized in terms of column type, mobile phase composition and flow rate. To improve peak capacity and obtain symmetric peak shape of alkaloids, a polar group embedded C18 column combined with chaotropic salts was used in the first dimension. To remove the disturbance of non-alkaloids, achieve unique selectivity and acquire symmetric peak shape of alkaloids, an SCX column combined with phosphate buffer was used in the second dimension. Method validation was performed in terms of linearity, precision (0.54–0.82%), recovery (94.1–105.2%), limit of detection (LOD) and limit of quantification (LOQ) of the three analytes varied between 0.067–0.115mgL −1 and 0.195–0.268mgL −1 , respectively. The method demonstrated superiority over 1DLC method in respect of resolution (less alkaloid co-eluted), sample preparation (no pretreatment procedure) and transfer rate (minimum component loss). The optimized RP – SCX 2DLC approach was subsequently applied to quantify target alkaloids in five herbal medicines and herbal medicine tablets from three different manufactures. The results demonstrated that the developed heart cutting RP – SCX 2DLC approach represented a new, strategically significant methodology for the quality evaluation of tropane alkaloid in related herbal medicines that involve complex chemical matrix. Highlights A heart cutting RP-SCX 2DLC method was optimized for determining tropane alkaloids. The developed method provided more accurate measurement of tropane alkaloids than 1DLC method. It offered a good precision (0.82–1.54%) and recovery (94.1–105.2%). It was sensitive (LOD: 0.067–0.115mgL −1 and LOQ: 0.195–0.268mgL −1 ). It was broadly applicable with the potential for the quantitative assessment of tropane alkaloids in herbal medicines and herbal medicine tablets.

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