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Journal of chromatography. B, Analytical technolog... 19건

  1. [해외논문]   Editorial Board   SCI SCIE


    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. ii - ii , 2018 , 1570-0232 ,

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  2. [해외논문]   Simultaneous accelerated solvent extraction and hydrolysis of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid glucuronide in meconium samples for gas chromatography–mass spectrometry analysis   SCI SCIE

    Mantovani, Cinthia de Carvalho (Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580, 13B, 05508-900 São Paulo, SP, Brazil ) , Silva, Jefferson Pereira e (Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580, 13B, 05508-900 São Paulo, SP, Brazil ) , Forster, Guilherme (Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580, 13B, 05508-900 São Paulo, SP, Brazil ) , Almeida, Rafael Menck de (Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580, 13B, 05508-900 São Paulo, SP, Brazil ) , Diniz, Edna Maria de Albuquerque (Pediatrics Department, University Hospital of São Paulo, Av. Prof. Lineu Prestes 2565, 05508-900 São Paulo, SP, Brazil ) , Yonamine, Mauricio (Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, Univers)
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. 1 - 7 , 2018 , 1570-0232 ,

    초록

    Abstract Cannabis misuse during pregnancy is associated with severe impacts on the mother and baby health, such as newborn low birth weight, growth restriction, pre-term birth, neurobehavioral and developmental deficits. In most of the cases, drug abuse is omitted or denied by the mothers. Thus, toxicological analyzes using maternal-fetal matrices takes place as a suitable tool to assess drug use. Herein, meconium was the chosen matrix to evaluate cannabis exposure through identification and quantification of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic (THCCOOH). Accelerated solvent extraction (ASE) was applied for sample preparation technique to simultaneously extract and hydrolyze conjugated THCCOOH from meconium, followed by a solid-phase extraction (SPE) procedure. The method was developed and validated for gas chromatography–mass spectrometry (GC–MS), reaching hydrolysis efficiency of 98%. Limits of detection (LOD) and quantification (LOQ) were, respectively, 5 and 10 ng/g. The range of linearity was LOQ to 500 ng/g. Inter and intra-batch coefficients of variation were cannabis exposure. Highlights Analysis of the major metabolite of THC in a maternal-fetal biological matrix Simultaneous extraction and hydrolysis of THCCOOH in meconium samples Feasibility of a “green” procedure in toxicological analysis of cannabinoids

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [해외논문]   Hydrophilic interaction liquid chromatography in the speciation analysis of selenium   SCI SCIE

    Sentkowska, Aleksandra (University of Warsaw, Heavy Ion Laboratory, Pasteura 5A, 02-093 Warsaw, Poland ) , Pyrzynska, Krystyna (University of Warsaw, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland)
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. 8 - 15 , 2018 , 1570-0232 ,

    초록

    Abstract The hydrophilic interaction liquid chromatography (HILIC) coupled to mass spectrometry was employed to study retention behavior of selected selenium compounds using two different HILIC stationary phases: silica and zwitterionic. Two organic solvents – acetonitrile and methanol – were compared as a component of mobile phase. Separation parameters such as a content of organic modifier, the eluent pH and inorganic buffer concentration were investigated. Based on all observations, methanol seems to be beneficial for the separation of studied compounds. The optimal HILIC separation method involved silica column and eluent composed of 85% MeOH and CH 3 COONH 4 (8 mM, pH 7) was compared to RP method in terms of time of the single run, the separation efficiency and limit of detection.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  4. [해외논문]   Determination of IMM-H004 and its active glucuronide metabolite in rat plasma and Ringer's solution by ultra-performance liquid chromatography-tandem mass spectrometry   SCI SCIE

    Jiang, Jianwei (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China ) , Zhang, Ziqian (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China ) , Zou, Xiaowen (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China ) , Wang, Rui (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China ) , Bai, Jie (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Xiannongtan Street, Beijing 100050, China ) , Zhao, Shengyu (Department of Drug Metabolism, Institute of Materia Medica, Chinese Academy of Medical Sciences and Pekin) , Fan, Xiaoqing , Sheng, Li , Li, Yan
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. 16 - 24 , 2018 , 1570-0232 ,

    초록

    Abstract IMM-H004 is a novel neuroprotective agent and its glucuronide metabolite IMM-H004G has similar protective effects against cerebral ischemic injury in vivo and in vitro . A specific and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method was established and validated for determination of IMM-H004 and IMM-H004G simultaneously in rat plasma and Ringer's solution. Plasma samples containing IMM-H004, IMM-H004G and internal standard propranolol were prepared by direct protein precipitation in a sample-to-solvent ratio of 1:2:6 (plasma: water: acetonitrile), whereas no protein precipitation was required for Ringer's solution samples. Separation was performed with a gradient mobile phase of methanol/water with 0.5% formic acid (v/v) on Eclipse Plus C18 column (2.1×50mm, 3.5μm) at a flow rate of 0.3mL/min. The detection was operated on a triple quadrupole mass spectrometer in positive ion multiple reaction monitoring (MRM) mode. The monitored transitions were 305.1→248.1 for IMM-H004, 481.3→305.1 for IMM-H004G and 260.1→183.1 for propranolol. The linear ranges of IMM-H004 and IMM-H004G were 5 to 3000ng/mL and 10 to 3000ng/mL for plasma method and 0.5 to 500ng/mL for Ringer's solution method. All the intra-day and inter-day precision and accuracy for the two analytes in rat plasma were below 7.5% and the intra-day precision and accuracy for analytes in Ringer's solution were within ±14.7%. There was no obvious matrix effect and the recoveries of the analytes were higher than 94.2%. IMM-H004 and IMM-H004G were stable during one analytic process. The established method was applied successfully to plasma pharmacokinetic and brain microdialysis studies of IMM-H004 and IMM-H004G in rats after a single intravenous administration of IMM-H004.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Effect of aspirin on the pharmacokinetics and absorption of panax notoginseng saponins   SCI SCIE

    Tian, Zhihao (Corresponding authors. ) , Pang, Huanhuan (Corresponding authors.) , Zhang, Qiang , Du, Shouying , Lu, Yang , Zhang, Lin , Bai, Jie , Li, Pengyue , Li, Danqi , Zhao, Mengdi , Chen, Xiaonan
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. 25 - 33 , 2018 , 1570-0232 ,

    초록

    Abstract Background Panax notoginseng saponins, a traditional Chinese medicine extraction, and aspirin are both widely used to treat cerebral infarction in China. Good results in clinical practice have been achieved, when Panax notoginseng saponins was taken together with aspirin. Methods To investigate the interaction of the two drugs in vivo, the concentration of notoginsenoside R 1 , ginsenoside Rg 1 , Rb 1 , Re and Rd. in blood were simultaneously measured by UPLC/MS/MS. Sample preparation was carried out by the protein precipitation technique with an internal standard saikosaponin A standard. The separation of six components was achieved by using an ACQUITY UPLC ?BEH C18 column (1.7μm 2.1×100mm) by gradient elution using water (containing 0.2% formic acid) and acetonitrile (containing 0.2% formic acid) as the mobile phase at a flow rate of 0.2mL/min. The pharmacokinetic parameters were determined using non-compartmental analysis. The transport of notoginsenoside R 1 , ginsenoside Rg 1 , Rb 1 , Re and Rd. in MDCK -MDR1 cell monolayer was also used to verify the conclusion of pharmacokinetic drug-drug interaction and study the mechanism of drug interaction. Results The concentrations of the five components increased in a certain extent when the two drugs administered together in rats. The values of apparent permeability coefficients were significantly increased when the two drugs were used together. Aspirin and salicylic acid could destroy the tight junction protein and open the intercellular space to increase the absorption of Panax notoginseng saponins. Conclusion Pharmacokinetic drug-drug interaction in vivo existed between Panax notoginseng saponins and aspirin. The drug-drug interaction mainly occurred in the process of absorption. Highlights In summary, this is the first report to evaluate the effect of aspirin on the pharmacokinetics of panax notoginseng saponins in rats. The pharmacokinetic curve and Cmax of Notoginsenoside R1(NGR1) and ginsenoside Rg1(GRg1), Rb1(GRb1), Re(GRe), Rd(GRd) show marked increase when the drugs were taken together. MDCK-MDR1 was also used to study the mechanism of ASA and SA in promoting the absorption of PNS. ASA or SA had a disruptive effect on the configuration and integrity of tight junction (TJ) proteins.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [해외논문]   Size exclusion chromatography (SEC-HPLC) as an alternative to study thrombin inhibition   SCI SCIE

    Pé (Universidad Autónoma del Estado de Hidalgo, Área Académica de Química, Ciudad del Conocimiento, Carretera Pachuca-Tulancingo km 4.5, Colonia Carboneras, CP 42184 Mineral de la Reforma, Hidalgo, Mexico ) , rez-Escalante, Emmanuel (Universidad Autónoma del Estado de Hidalgo, Área Académica de Química, Ciudad del Conocimiento, Carretera Pachuca-Tulancingo km 4.5, Colonia Carboneras, CP 42184 Mineral de la Reforma, Hidalgo, Mexico ) , Gonzá (Universidad Autónoma Metropolitana, Unidad Iztapalapa, División de Ciencias Biológicas y de la Salud, Departamento de Biotecnología, Av. San Rafael Atlixco 186, Colonia Vicentina, CP 09340 Ciudad de México, Mexico ) , lez-Olivares, Luis Guillermo (Universidad Autónoma del Estado de Hidalgo, Área Académica de Química, Ciudad del Conocimiento, Carretera Pachuca-Tulancingo km 4.5, Colonia Carboneras, CP 42184 Mineral de la Reforma, Hidalgo, Mexico ) , Cruz-Guerrero, Alma Elizabeth (Universidad Autónoma del Estado de Hidalgo, &Aacu) , Galá , n-Vidal, Carlos André , s , Pá , ez-Herná , ndez, Ma. Elena , Á , lvarez-Romero, Giaan Arturo
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. 34 - 38 , 2018 , 1570-0232 ,

    초록

    Abstract In vitro analysis of anticoagulant compounds with a potential use as antithrombotic drugs, has been traditionally performed using techniques like spectrophotometry, turbidimetry, as well as electrochemical and clinical assays. Although, these techniques have some disadvantages such as: the inability to measure the total biological activity of thrombin, interferences and, sometimes, the quantitative determination of the inhibition ratio is not accurate. In the present work, the conversion of fibrinogen to fibrin was monitored by molecular exclusion chromatography (SEC-HPLC) in three different reaction systems. An inhibition percentage of 43.19±2.02% was obtained using heparin as an anticoagulant, in addition to the determination of the percentage of heparin bonded to thrombin. This methodology has not been previously described and has high potential for the determination of anticoagulant capacity with higher precision, the determination of thrombin's total biological activity and the quantitative determination of the inhibition ratio. Highlights New chromatographic methodology for the determination of anticoagulant capacity. SEC-HPLC analysis of the fibrinogen - fibrin conversion process. Interference analysis during the fibrinogen - fibrin conversion process. Heparin inhibition percentage determination by means of SEC-HPLC. Graphical abstract [DISPLAY OMISSION]

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [해외논문]   A sensitive and selective immunoaffinity column clean up coupled to UPLC-MS/MS for determination of trace methyl-3-quinoxaline-2-carboxylic acid in animal tissues   SCI SCIE

    Li, Peipei (Marine Fishery Institute of Zhejiang Province, Zhoushan 316021, Zhejiang Province, China ) , Zhang, Xiaojun (Marine Fishery Institute of Zhejiang Province, Zhoushan 316021, Zhejiang Province, China ) , Zhang, Juanmei (Pharmaceutical College of Henan University, Kaifeng 475001, Henan Province, China ) , Yan, Zhongyong (Marine Fishery Institute of Zhejiang Province, Zhoushan 316021, Zhejiang Province, China ) , Zhang, Shuai (Marine Fishery Institute of Zhejiang Province, Zhoushan 316021, Zhejiang Province, China ) , Chen, Si (Marine Fishery Institute of Zhejiang Province, Zhoushan 316021, Zhejiang Province, China ) , Fang, Yi (Marine Fishery Institute of Zhejiang Province, Zhoushan 316021, Zhejiang Province, China)
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. 39 - 45 , 2018 , 1570-0232 ,

    초록

    Abstract This paper described a reliable and simple method for the selective determination of MQCA in animal tissues using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). A highly targeted immunoaffinity column was used for sample purification after enzymatic hydrolysis. The purified extracts were analyzed by reversed-phase HPLC–MS/MS in positive ESI and multiple reaction monitoring mode. The calibration curves showed good linearity with correlation coefficient (r 2 ) larger than 0.995. The average recoveries at the spiked levels of 0.5, 2.0 and 20μgkg −1 were 90.2% to 103.5% with intra-day and inter-day relatives standard deviations (RSD, n=6) ranging from 1.8% to 6.7% and 3.5% to 7.6% respectively. The limit of quantification (LOQ) was 0.5μgkg −1 , which can fulfil the maximum residue level (MRL) of 4.0μgkg −1 stipulated by the Agricultural Minister of China and the requirement of the confirmatory criteria according to the European Commission Decision 2002/657/EC. The method is sensitive, accurate, convenient and rapid, and has been successfully applied in real samples.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [해외논문]   Preliminary investigation of human exhaled breath for tuberculosis diagnosis by multidimensional gas chromatography – Time of flight mass spectrometry and machine learning   SCI SCIE

    Beccaria, Marco (Thayer School of Engineering, Dartmouth College, Hanover, NH, USA ) , Mellors, Theodore R. (Thayer School of Engineering, Dartmouth College, Hanover, NH, USA ) , Petion, Jacky S. (Groupe Haitien d'Etude du Sarcome de Kaposi et des Infections Opportunistes (GHESKIO), Port-au-Prince, Haiti ) , Rees, Christiaan A. (Geisel School of Medicine, Dartmouth College, Hanover, NH, USA ) , Nasir, Mavra (Geisel School of Medicine, Dartmouth College, Hanover, NH, USA ) , Systrom, Hannah K. (Geisel School of Medicine, Dartmouth College, Hanover, NH, USA ) , Sairistil, Jean W. (Groupe Haitien d'Etude du Sarcome de Kaposi et des Infections Opportunistes (GHESKIO), Port-au-Prince, Haiti ) , Jean-Juste, Marc-Antoine (Groupe Haitien d'Etude du Sarcome de Kaposi et des Infections Opportunistes (GHESKIO), Port-au-Prince, Haiti ) , Rivera, Vanessa (Groupe Haitien d'Etude du Sarcome de Kaposi et des Infections Opportunistes (GHESKIO), Port-au-Prince, Haiti ) , Lavoile, Kerline (Groupe Haitien d'Etude du Sarcome de Kaposi et des Infections Opportunistes (GHESKIO), Port-au-Prince, Haiti ) , Severe, Patrice (Groupe Haitien d'Etude du Sarcome de Kaposi et des Infections Opportunistes (GHESKIO), Port-au-Prince, Haiti ) , Pape, Jean W. (Gro) , Wright, Peter F. , Hill, Jane E.
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. 46 - 50 , 2018 , 1570-0232 ,

    초록

    Abstract Tuberculosis (TB) remains a global public health malady that claims almost 1.8 million lives annually. Diagnosis of TB represents perhaps one of the most challenging aspects of tuberculosis control. Gold standards for diagnosis of active TB (culture and nucleic acid amplification) are sputum-dependent, however, in up to a third of TB cases, an adequate biological sputum sample is not readily available. The analysis of exhaled breath, as an alternative to sputum-dependent tests, has the potential to provide a simple, fast, and non-invasive, and ready-available diagnostic service that could positively change TB detection. Human breath has been evaluated in the setting of active tuberculosis using thermal desorption-comprehensive two-dimensional gas chromatography–time of flight mass spectrometry methodology. From the entire spectrum of volatile metabolites in breath, three random forest machine learning models were applied leading to the generation of a panel of 46 breath features. The twenty-two common features within each random forest model used were selected as a set that could distinguish subjects with confirmed pulmonary M. tuberculosis infection and people with other pathologies than TB. Highlights For the first time a GC×GC system was used to evaluate human breath in the setting of tuberculosis disease Three random forest models were applied for data elaboration Twenty-two common features within each random forest model used were selected as a set to distinguish between infected and control group

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  9. [해외논문]   Urinary metabolomics study the mechanism of Taohong Siwu Decoction intervention in acute blood stasis model rats based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry   SCI SCIE

    Zhang, Xiaosong (Corresponding author.) , Li, Pengling , Hua, Yongli , Ji, Peng , Yao, Wanling , Ma, Qi , Yuan, Ziwen , Wen, Yanqiao , Yang, Chaoxue , Wei, Yanming
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. 51 - 60 , 2018 , 1570-0232 ,

    초록

    Abstract Taohong Siwu Decoction (TSD) is a classic prescription in traditional Chinese medicine and is widely used to promote blood circulation to remove blood stasis. However, the effect mechanisms are not yet well understood. Here, a urinary metabolomic approach based on liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (LC/Q-TOF-MS) was conducted to explore the changes in the endogenous metabolites and to assess the integral efficacy of TSD on acute blood stasis model rats. Then, parameters for hemorheology and coagulation functions were detected. Principal component analysis (PCA) and orthogonal partial least squares discriminate analysis (OPLS-DA) was used to investigate the global metabolite alterations and to evaluate the preventive effects of TSD in rats. Potential metabolite markers were found using OPLS-DA and t -test. Furthermore, metabolic pathway analysis was performed to construct metabolic networks. The results showed that TSD could significantly decrease whole blood viscosity and plasma viscosity. It also significantly prolonged partial thromboplastin time (APPT) and prothrombin time (PT), increased thrombin time (TT) and lowered fibrinogen content (FIB). Moreover, 24 potential metabolite markers of acute blood stasis were screened, and the levels were all reversed to different degrees after TSD administration. In metabolic networks, amino acid metabolism (arginine and proline metabolism; histidine metabolism; alanine, aspartate, and glutamate metabolism; phenylalanine, tyrosine, and tryptophan biosynthesis; phenylalanine metabolism) and lipid metabolism (glycerophospholipid metabolism; linoleic acid metabolism; alpha-linolenic acid metabolism) were closely related with the intervention mechanism of TSD on acute blood stasis. The urinary metabolomic approach can be applied to clarify the mechanism of TSD in promoting blood circulation to remove acute blood stasis and to provide the theoretical basis for further research on the therapeutic mechanism of TSD in clinical practice. Highlights Based on urinary metabolomics to explain the intervention mechanism of TSD in acute blood stasis model rats. The PCA and OPLS-DA statistical analysis method was used to investigate the global metabolite alterations in this study. The preventive mechanism of TSD in acute blood stasis may mainly involve in regulating the lipid and amino acid metabolism.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  10. [해외논문]   Development of a method to determine axitinib, lapatinib and afatinib in plasma by micellar liquid chromatography and validation by the European Medicines Agency guidelines   SCI SCIE

    Albiol-Chiva, Jaume (Departament de Química Física i Analítica, Universitat Jaume I, 12071 Castelló, Spain ) , Esteve-Romero, Josep (Departament de Química Física i Analítica, Universitat Jaume I, 12071 Castelló, Spain ) , Peris-Vicente, Juan (Departament de Química Física i Analítica, Universitat Jaume I, 12071 Castelló, Spain)
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences v.1074 ,pp. 61 - 69 , 2018 , 1570-0232 ,

    초록

    Abstract A method based on micellar liquid chromatography to quantify the tyrosine kinase inhibitors axitinib, lapatinib and afatinib in plasma is reported. The sample pretreatment was a simple 1/5-dilution in a pure micellar solution, filtration and direct injection, without requiring extraction or purification steps. The three drugs were resolved from the matrix in 17min, using an aqueous solution of 0.07M sodium dodecyl sulfate – 6.0% 1-pentanol, buffered at pH7 with 0.01M phosphate salt as mobile phase, running under isocratic mode at 1mL/min through a C18 column. The detection was performed by absorbance at 260nm. An accurate mathematical relationship was established between the retention factor of each drug and the surfactant/organic solvent concentration in the mobile phase, achieved with a limited number of experiments, in order to optimize these factors. A binding behavior of the analytes face to the micelles was found out. The method was successfully validated by the guidelines of the European Medicines Agency in terms of: selectivity, linearity ( r 2 >0.9995), calibration range (0.5 to 10mg/L), limit of detection (0.2mg/L), carry-over effect, accuracy (−8.1 to +6.9%), precision ( Highlights We determine three anticancer drugs in plasma by micellar liquid chromatography. The sample pretreatment consists of a one-step dilution and direct injection. The mobile phase was effortless optimized using a regression model. The method was validated by the guidelines of the European Medicines Agency. It was inexpensive, rapid, eco-friendly, safe, and useful for routine analysis.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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