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H : 소장처정보

T : 목차정보

한국연초학회지 = Journal of the Korean society of tobacco... 12건

  1. [국내논문]   담배, 토마토 및 고추에서 분리된 TMV 계통의 생물학적, 물리화학적 및 혈청학적 특성   피인용횟수: 1

    박은경 (한국인삼연초연구원 ) , 이청호 (한국인삼연초연구원 ) , 이영기 (한국인삼연초연구원 ) , 김영호 (한국인삼연초연구원)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 5 - 10 , 1997 , 1225-2131 ,

    초록

    Three strains of W isolated from tobacco, tomato and Pepper plants in Korea were characterized based on biological response, serological relationship, and peptide mapping of the capsid Proteins. The strains designated as TMV-common, TMV-Pepper, and TMV-tomato could be distinguishable by different visual symptoms on 3 varieties of tobacco, one variety of tomato and Pepper for each among 27 plant specieces. Serological relationships were examined by agar gel double diffusion test. Only traceable or weak reaction was observed in the incompatible antigen-antibody combinations. The Pepper strain, however, showed trace in reaction with other two antisera. Peptide maps of the capsid proteins digested by V8 protease or by trypsin were also distinguishable, suggesting differences in composition and/or sequence of the amino acids among the strains.

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  2. [국내논문]   감자 바이러스 Y 복제유전자 cDNA로 형질전환된 황색종 담배의 저항성 특성  

    박은경 (한국인삼연초연구원 ) , 백경희 (고려대학교 생명공학원 ) , 유진삼 (고려대학교 생명공학원 ) , 조혜선 (생명공학연구소 ) , 강신웅 (한국인삼연초연구원 ) , 김영호 (한국인삼연초연구원)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 11 - 17 , 1997 , 1225-2131 ,

    초록

    A flue-cured tobacco variety (Nicotiana tabacum cv. Wisconsin) was used for Plant transformation with the complementary DNA (cDNA) of potato virus Y-necrosis strain (PVY-VN) replicase gone (Nb) which was synthesized through reverse-transcription Primed with oligo(dT) and Polymerization using RNase H-digested template. The cDNA was cloned into Plant expression vector Plasmid (PMBP2), and introduced into tobacco plants by co-culturing tobacco leaf disks with Agrobacterium tumefaciens LBA4404 containing the plasmid before Plant regeneration. Eight Plants, in which the inserted cDNA fragment was detected by Polymerase chain reaction (PCR), out of 70 putative transformants inserted with sense-oriented Mb cDNA showed no symptom at 3 weeks after inoculation, while the other 62 plants, and all plants with vector gone only and antisense-oriented NIb cDNA had susceptible vein-necrosis symptoms. However, only 2 of the 8 resistant plants were highly resistant, which remained symptomless up to 10 weeks after inoculation. Among the first progenies (T1) from self-fertilized seeds of the two resistant transgenic plants, less than 10 % of 71 plants appeared highly resistant (with no symptom), 70% moderately resistant (with mild symptoms on 1 - 2 leaves), and about 20% susceptible (with susceptible symptoms on 3 or more leaves) at 3 weeks after inoculation. These results suggest that the PVY resistance was inherited in the 71 generation. Key words : potato virus Y. viral replicase gene, transgenic tobacco Plants, resistance.

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  3. [국내논문]   육묘포트 깊이가 담배의 뿌리발달 및 분포에 미치는 영향  

    이상각 (고려대학교 자연자원대학 ) , 심상인 (고려대학교 자연자원대학 ) , 강병화 (고려대학교 자연자원대학 ) , 이학수 (충북대학교 농과대학 ) , 석영선 (충북대학교 농과대학)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 18 - 23 , 1997 , 1225-2131 ,

    초록

    The study was carried out to clarify reasonable production of healthy seedling, optimal Pot depth, and appropriate transplanting time, which can be deduced from understanding of seedling quality. Seedling quality results from growth of root and shoot, morphology and distribution of root system under influence of Pot depth during seedling growing period. Stem height, shoot dry weight, leaf area and leaf number were increased in proportion to depth of pots. Growth of shoot and root during seedling growing period showed the most dramatic development between 20th and 25th day after temporary planting. Root number increased as pot depth decrease and total root length and dry weight increased as pot depth increase. In 5cm pot, relative multiplication rate was higher and mean extension rate was lower than other depth of Pot. The limitation of pot volume in which rhizosphere was located enhance the development of roots of second and third order. At 20th days after temporary Planting root distribution was relatively uniform in length and development of adventitious root on stem base was poor as Pot depth decreased.

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  4. [국내논문]   사료작물 윤작재배에 의한 연초포지의 제염효과  

    이철환 (한국인삼연초연구원 대구시험장 ) , 진정의 (한국인삼연초연구원 대구시험장 ) , 한철수 (한국인삼연초연구원 대구시험장)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 24 - 28 , 1997 , 1225-2131 ,

    초록

    The field experiment for field desalinization by precultivation of orchard crops were carried out to evaluate relationship between the varieation of chlorine contents of soil and crop uptake in the upland diverted from paddy field. After harvest of grass crops, soil samples were taken for analysis of chlorine contents of soil layers. Regardless of kinds of grass crops cutivated, contents of soil chlorine were decreased comparing to non-crop plot. Chlorine content in plant harvested at just before the flowering stage was much higher than that of after flowering. Chlorine uptake and dry matter were increased in order of Italian ryegrass, Perennial ryegrass, and Sudan grass. Positive correlations were showed between chlorine uptake and dry matter. The content of soil chlorine decreased by higher yield of dry matter.

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  5. [국내논문]   버어리종 잎담배 대말림시 수평대걸기에 관한 연구   피인용횟수: 1

    배성국 (한국인삼연초연구원 전주시험장 ) , 김요태 (한국인삼연초연구원 전주시험장 ) , 임해건 (한국인삼연초연구원 전주시험장 ) , 조천준 (한국인삼연초연구원 전주시험장)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 29 - 32 , 1997 , 1225-2131 ,

    초록

    This study was carried out to develop a new stalk curing of the horizontal hanging method that would reduce the harvesting and curing labor, and improve its quality of cured leaf than the conventional stalk curing method in burley tobacco. The horizontal hanging stalk-curing method designed from 1988 was compared with the conventional method. The horizontal method shortened the curing Period for 7 days and reduced to 0.3%, while it occurred 9.4% in the vertical method. In the horizontal method, the quality of cured leaf improved 9%, but the contents of chemical components and physical properties were similar to those of vertical method. The labor of stalk-cut and loading in curing facility reduced 28% by the horizontal method. In harvesting and curing process, the horizontal method resulted 12% of labor saving as compared with the vertical method. Key words : horizontal hanging stalk-curing method, vertical method, stalk-curing.

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  6. [국내논문]   시비방법이 버어리종 잎담배의 농경적 특성에 미치는 영향  

    조천준 (한국인삼연초연구원 전주시험장 ) , 배성국 (한국인삼연초연구원 전주시험장 ) , 임해건 (한국인삼연초연구원 전주시험장 ) , 김요태 (한국인삼연초연구원 전주시험장)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 33 - 39 , 1997 , 1225-2131 ,

    초록

    Recently the method of basal compound fertilizer (N : P : K = 18.2 : 9.8 : 35.0) Placement has been changed from banding to broadcasting in tobacco cultivation. The effects of the compound fertilizer placement, level of fertilizer and additional urea application on the agronomic characteristics, two chemical compositions and physical Properties of burley tobacco(Nicotiana tabacum L. cv. KB 108) were investigated at Chonju Experiment Station, Korea Ginseng and Tobacco Research Institute in 1995-1996. Six treatments consisted of (1) band (method of fertilizer Placement) + In kg/10a(compound fertilizer) + 0(additional urea applied), (2) broadcast+140+0, (3) broadcast+140+25, (4) band+180+0, (5) broadcast+180+0, and (6) broadcast +180+25. The additional urea was applied at hilling. No significant differences were detected between banding and broadcasting method of compound fertilizer Placement in field 9rowth, wield, organoleptic qualify(price Per kilogram), chemical composition and Physical properties of cured leaf, The increased compound fertilizer by 30%(40k/10a) or the additional urea application by 25kg per 10a produced slightly higher yield than the recommended amount of basal compound fertilizer without additional urea application did. It also increased the total alkaloid content of cured leaf. It is recommended that no more basal fertilizer above the recommended amount and no additional urea application are needed in burley tobacco fertilization, even though the method of basal fertilizer placement being chanced from banding to broadcasting. Key words : Nicotiana tabacum, fertilizer placement, additional urea.

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  7. [국내논문]   흡연자 및 비흡연자의 뇨중 니코틴 및 코티닌 함량  

    이문수 (한국인삼연초연구원 분석부 ) , 나도영 (한국인삼연초연구원 분석부 ) , 황건중 (한국인삼연초연구원 분석부)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 40 - 45 , 1997 , 1225-2131 ,

    초록

    This study was conducted to evaluate the personal effects of tobacco smoke and environmental tobacco smoke(ETS) by measuring the concentration of nicotine and cotinine in the urine. While 129 urine samples were being collected, Personal characteristics such as sex, age, number of years since a Person has been a smoker, average consumption number of cigarettes per day, and number of smoker in family were also surveyed. Collected urine samples were used for analysis of nicotine and cotinine by GC/NPD after Passing the extrelut column. In the urine of the smoker, the average contents of nicotine and cotinine were 5.38 $\mu\textrm{g}$ /ml and 3.14 $\mu\textrm{g}$ /ml, respectively. The average contents of nicotine and cotinine were 0.18 $\mu\textrm{g}$ /ml and 0.07 $\mu\textrm{g}$ /ml in the urine of male non-smoker, respectively. The contents of nicotine and cotinine in the non-smoker's urine were dependent on sex and age. On the other hand, the contents of nicotine and cotinine in smoker's urine were dependent on average consumption amount of cigarettes per day. Also, there was a direct relation between nicotine levels in the smoker's urine and the average consumption number of cigarettes Per day of smoker. The Possible sources of nicotine and cotinine in the non-smoker's urine seemed to be caused by food, beverage and En, Our results indicate that the number of smoker in family had no effect on increasing nicotine and cotinine contents in the urine of non-smoker.

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  8. [국내논문]   활성탄의 pH에 따른 Ammonia, Hydrogen Sulfide 및 Methylmercaptan 흡착 특성   피인용횟수: 2

    김정열 (한국인삼연초연구원 ) , 신창호 (한국인삼연초연구원 ) , 서문원 (한국인삼연초연구원 ) , 김종열 (한국인삼연초연구원 ) , 김영호 (한국인삼연초연구원 ) , 이근회 (한국인삼연초연구원)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 46 - 50 , 1997 , 1225-2131 ,

    초록

    The pH of coconut based activated carbon was changed by treating with NaOH and HNO3 and we have calculated the adsorption amounts of ammonia, hydrogen sulfide and methylmercaptan on the activated carbons using the break-through time which was obtained from break-through curve experiments. As a result of this study, the adsorption amounts of ammonia Has on the activated carbons were 2,6 mg/g, 17.2 mg/g and 31.6 mg/g with the pH 11, pH 7 and pH 3, respectively. These results indicated that the adsorption ability of ammonia on activated carbon was increased with decreasing the pH of activated carbon. Otherwise, in the cases of the adsorption experiment of hydrogen sulfide and methylmercaptan on the activated carbons with different pH. the activated carbon with pH 11 showed higher adsorption capacity than the activated carbons with pH 7 and pH 3. The adsorption amounts of hydrogen sulfide and methylmercaptan were 39.9 mg/g and 178 mg/g with pH 11, respectively. Finally, we analyzed the amount of ammonia delivered from 88 Lights cigarette made of triple filter which contained the activated carbon. The amount of ammonia delivered to smoke from the filter cigarette containing the activated carbon with pH 3 was 45.1 ${\mu}g$ /cig. This value was lower 23.8 % than that from the activated carbon with the pH 11.

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  9. [국내논문]   오리엔트 잎담배 부산물(scraps)을 이용한 판상엽 제조  

    김용옥 (한국인삼연초연구원 ) , 김기환 (한국인삼연초연구원 ) , 김천석 (한국인삼연초연구원 ) , 박영수 (한국인삼연초연구원 ) , 이태호 (한국인삼연초연구원)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 51 - 56 , 1997 , 1225-2131 ,

    초록

    This experiment was conducted to reduce the cost of orient leaf using izmir, samsun and basma scraps in cigarettes manufacturing process. We manufactured rolled and paper sheet tobacco using orient scraps and substituted orient leaf in Pine tree to the rolled and paper sheet tobacco using orient scraps. Orient scraps were lower in total sugar, total sugar/nicotine and total nitrogen/nicotine, higher in nicotine and crude ash than those of orient leaves. Izmir and basma scraps were higher of 2-methyl butanoic, 3-methyl butanoic and 3-methyl pentanoic acid, but the chemical contents in samw scraps were lower than those of orient leaves. The specific gravity thickness, flness index, filling value were similar between orient scraps blended sheet tobacco and present sheet tobacco. The physical characteristics of orient scraps blended sheet tobacco were within Present sheet tobacco quality specification. The sheet tobacco using orient scraps was higher 2-metal butanoic, 3-methyl butanoic and 3-methyl pentanoic acid than those of present sheet tobacco. The rolled processed sheet tobacco using orient scraps was higher 2-methyl butanoic, 3-methyl butanoic and 3-methyl Pentanoic acid than those of paper sheet tobacco using orient scraps. The sample cigarettes using orient scraps sheet tobacco were lower 2-methyl butanoic, 3-mettwl butanoic and 3-mettwl Pentanoic acid than those of control cigarettes. The result of sensory test showed no great difference compared with sample of orient scraps and control cigarettes. This result shows that we can possible to substitute orient leaf to orient scraps to reduce the cost of orient leaf.

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  10. [국내논문]   황색종 잎담배의 엽면지질 성분 조성 및 건조중 변화   피인용횟수: 1

    지상운 (한국인삼연초연구원 분석부 ) , 김도연 (한국인삼연초연구원 분석부 ) , 이문용 (한국인삼연초연구원 분석부 ) , 김영희 (한국인삼연초연구원 분석부 ) , 신승구 (한국인삼연초연구원 음성시험장)
    한국연초학회지 = Journal of the Korean society of tobacco science v.19 no.1 ,pp. 57 - 63 , 1997 , 1225-2131 ,

    초록

    The composition of leaf surface lipid in Flue-cured tobacco and their changes during curing was investigated. The flue-cured variety, Nicotiana tabacum cv. NC 82 was cultivated at Eumsung experiment station in 1996. The samples of riced leaves with different stalk Position(Lugs, Cutter, Leaf and Tips) and different curing stage at half yellowing(24hr), yellowing(48 hr) , color axing(72 hr), midrib drying(96 hr) and cured(120 hr) were collected for analysis of leaf surface lipid. $\alpha$ - and $\beta$ - 4, 8, 13-Duvatriene-1, 3-diol( $\alpha$ , $\beta$ -DVT) were major components in leaf surface lipid extracted with methylene chloride and sugar ester was detected slightly DVT content was increased with ascending stalk position, but increasing trend of total hydrocarbon was not observed. While DVT was decreased throughout curing of tobacco leaves, hydrocarbon content did not show significant change during curing process. Twenty-two duvane compounds were detected by capillary GC in duvane fraction isolated from leaf surface lipid and of which 11 compounds were identified by GC-MS. These compounds were decreased with curing in all stalk position.

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