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大韓口腔顎顔面外科學會誌 = Journal of the Korean Association o... 24건

  1. [국내논문]   가토에서 자가유래 골아줄기세포를 이용한 상악동 골 이식술시 비계체로서 Bio-$Oss^{(R)}$의 효과에 관한 연구   피인용횟수: 1

    이준 (원광대학교 치과대학 구강악안면외과학교실 ) , 성대혁 (원광대학교 치과대학 구강악안면외과학교실 ) , 최재영 (원광대학교 치과대학 구강악안면외과학교실 ) , 최성림 (원광대학교 치과대학 구강악안면외과학교실 ) , 차수련 (원광대학교 치과대학 구강악안면외과학교실 ) , 장재덕 (세원 셀론텍 RMS-Bio Net ) , 김은철 (원광대학교 치과대학 구강악안면병리학교실)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 405 - 418 , 2007 , 1225-1585 ,

    초록

    Mesenchymal stem cells(MSCs) have been though to be multipotent cells that can replicate that have the potential to differentiate into lineages of mesenchymal tissue including the bone, cartilage, fat, tendon, muscle, and marrow stroma. Especially, scaffolds to support cell-based tissue engineering are critical determinants of clinical efforts to regenerate and repair the body. Selection of a matrix carrier imvolves consideration of the matrix's role as a scaffold for physical support and host tissue integration as well as its ability to support of synergize the osteoinductive program of the implanted mesenchymal stem cell. The aim of this study is to evaluate the effect of autobone and Bio- $Oss^{(R)}$ to adherent mesenchymal stem cells as scaffolds on sinus augmentation with fibrin glue mixture in a rabbit model. 16 New Zealand White rabbits were divided randomly into 4 groups based on their time of sacrifice(1, 2, 4 and 8 weeks). First, mesenchymal stem cells were isolated from iliac crest marrow of rabbits and expanded in vitro. Cell culture was performed in accordance with the technique described by Tsutsumi et al. In the present study, the animals were sacrificed at 1, 2, 4 and 8 weeks after transplantation, and the bone formation ability of each sides was evaluated clinically, radiologically, histologically and histomorphologically. According to the histological observations, autobone scaffolds group showed integrated graft bone with host bone from sinus wall. At 2 and 4 weeks, it showed active newly formed bone and neovascularization. At 8 weeks, lamellae bone was observed in sinus graft material area. Radiologically, autobone with stem cell showed more radiopaque than Bio- $Oss^{(R)}$ scaffolds group. there were significant differences in bone volume between 4 and 8 weeks(p

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  2. [국내논문]   나노 에멀젼이 잇몸 염증의 예방 및 치료에 미치는 효과에 대한 연구   피인용횟수: 1

    채창훈 (나노큐어텍 나노-바이오 퓨전 부설연구소 ) , 박준우 (한림대학교 의과대학 구강악안면외과학교실)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 419 - 425 , 2007 , 1225-1585 ,

    초록

    We have found out the relationship of nanoemulsion containing nano vitamin C, E and propolis and gingival disease. We've confirmed effect of nanoemulsion through the experiment of in vivo and in vitro. We tested cell viability of gingival fibroblast cells by MTT assay and mRNA appearance of interleukin- $1{\beta}$ , using mouse that was guided inflammation. Anti-microbacterial activity for Antibacterial effect's experiment was carried out by using S.aureus and E.coli. In addition, inflammation tissue has been observed with scanning electrical microscopy. In this study, expression of interleukin- $1{\beta}$ was decreased after adding nanoemulsion containing nanovitamin C, E and propolis. We've also obtained good results from the test of Antibacterial effect against S.aureus and E.coli. Also, swelling of inflammation tissues observed by scanning electrical microscopy has gone down. In conclusion, we have gained confidence that nanoemulsion containing nano vitamin C, E and propolis has very high Antibacterial effect against bacteria in oral. And it made us guess that inflammation of gingival reduces after decreasing interleukin- $1{\beta}$ . Thus, we expect that nanoemulsion containing nano vitamin C, E and propolis gives good effects to patient having gingival disease.

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  3. [국내논문]   구강 편평세포암종 세포주에서 Cyclosporin A와 Taxol 투여시 PI-3 kinase/Akt1 Pathway에 의한 세포사멸 병용효과  

    김규영 (단국대학교 치과대학 구강악안면외과학교실 ) , 이재훈 (단국대학교 치과대학 구강악안면외과학교실)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 426 - 436 , 2007 , 1225-1585 ,

    초록

    Oral cancer take up 2-6% of all carcinomas and squamous cell carcinoma, which is the most common type in oral cancer, has a poor prognosis due to its high metastasis and recurrence rates. In treating oral cancer, chemotherapy to the primary, metastasized and recurrent lesion is a very important and useful treatment, even though its widespread usage is limited due to high general toxicity and local toxicity to other organs. Taxol, a microtubule stabilizing agent, is an anticancer drug that induces cell apoptosis by inhibiting depolymerization of microtubules in between the metaphase and anaphase of the cell mitosis. Recently, its effectiveness and mechanism on various tumor has been reported. However, not much research has been done on the application of Taxol to oral squamous cell carcinoma. Cyclosporin A, which is an immunosuppressant, is being used on cancers and when co-administered with Taxol, effectiveness of Taxol is enhanced by inhibition of Taxol induced multidrug resistance. In this study, Cyclosporin A with different concentration of Taxol was co-administered to HN22, the oral squamous cell carcinomacell line. To observe the cell apoptosis and the mechanisms that take part in this process, mortality evaluation of tumor cell using wortmannin, c-DNA microarray, RT-PCR analysis, cytometry analysis and western blotting were used, and based upon the observation on the effect and mechanism of the agent, the following results were obtained: 1. The HN22 cell line viability was lowest when $100{\mu}M$ of Wortmannin and $5{\mu}g/ml$ of Taxol were co-administered, showing that Taxol participates in P13K-AKT1 pathway. 2. In c-DNA microarray, where $1{\mu}g/ml$ of cyclosporine A and 3mg/ml of Taxol were co-administered, no up regulation of AKT1, PTEN and BAD c-DNA that participate in cell apoptosis was observed. 3. When $1{\mu}g/ml$ of Cyclosporin A was applied alone to HN22 cell line, no difference was found in AKT1, PTEN and BAD mRNA expression. 4. Increased AKT1, mRNA expression was observed when $3{\mu}g/ml$ of Taxol was applied alone to HN22 cell line. 5. When $1{\mu}g/ml$ of Cyclosporin A and Taxol( $3{\mu}g/ml\;and\;5{\mu}g/ml$ ) were co-administered to HN22 cell line, PTEN mRNA expression increased, whereas AKT1 and BAD mRNA decreased. 6. As a result of cytometry analysis, in the group of Cyclosporin A( $1{\mu}g/ml$ ) and Taxol( $3{\mu}g/ml$ ) co-administration, increased Annxin V was observed, which shows that apoptosis occurred by deformation of plasma membrane. However, no significant difference was observed with vary ing concentration. 7. In western blot analysis, no caspase 3 was observed in the group of Cyclosporin A( $1{\mu}g/ml$ ) and Taxol( $3{\mu}g/ml$ ) co-administration. From the results of this study, it can be concluded that synergistic effect can be observed in combination therapy of Taxol and Cyclosporin A on oral squamous cell carcinoma cell line, where decreased activity of the cell line was observed. This resulted in decreased AKT1 and BAD mRNA and increased PTEN mRNA expression and when wortmannin and Taxol were co-administered, the viability decreased which confirms that Taxol decreases the viability of tumor cell line. Hence, when Taxol and cyclosporine A are co-administered, it can be assumed that cell apoptosis occurs through AKt1 pathway.

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  4. [국내논문]   인체상피세포를 이용한 Vinyl acetate의 발암성 및 작용기전  

    조준현 (경북대학교 치과대학 구강악안면외과학교실 ) , 김진수 (경북대학교 치과대학 구강악안면외과학교실)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 437 - 444 , 2007 , 1225-1585 ,

    초록

    Vinyl acetate has been widely used for the manufacture of polyvinyl alcohol emulsion, which is primary ingredient of adhesive, paints, textile, paperboard coatings, etc. Since these products are plentiful and frequently used around us, workers and consumers are at health risk. International Agency for Research on Cancer(IARC) classified vinyl acetate as group 2B(possibly carcinogenic to humans). Among the organs targeted, the oral cavity is the most vulnerable organ affected by the carcinogenic effects of vinyl acetate. Since the origin of most of oral cancer is derived from the epithelial cells, it is important to understand the carcinogenic potential of vinyl acetate in human epithelial cells. Thus, the present study has attempted to utilize the immortalized human epithelial cell model to assess the carcinogenic potency of this chemical and to understand the underlying mechanisms.

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  5. [국내논문]   Phosphatidic acid에 의한 intercellular adhesion molecule-1 발현 조절에 관여한 MAPK와 PKC-${\delta}$의 역할  

    조우성 (영남대학교 의과대학 치과학교실 ) , 윤홍식 (영남대학교 의과대학 치과학교실 ) , 진병로 (영남대학교 의과대학 치과학교실 ) , 백석환 (영남대학교 의과대학 생화학.분자생물학교실)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 445 - 454 , 2007 , 1225-1585 ,

    초록

    Background: Phosphatidic acid(PA), an important second messenger, is involved in inflammation. Notably, cell-cell interactions via adhesion molecules playa central role in inflammation. This thesis show that PA induces expression of intercellular adhesion molecule-1(ICAM-1) on macrophages and describe the signaling pathways. Materials and methods: Macrophages were cultured in the presence of 10% FBS and assayed cell to cell adhesion using HUVEC. For the gene and protein analysis, RT-PCR, Western blot and flow cytometry were performed. In addition, overexpressed cell lines for dominant negative PKC- ${\delta}$ mutant established and tested their effect on the promoter activity and expression of ICAM-1 protein by PA. Results: PA-activated macrophages significantly increased adhering to human umbilical vein endothelial cell and this adhesion was mediated by ICAM-1. Pretreatment with rottlerin(PKC- ${\delta}$ inhibitor) or expression of a dominant negative PKC- ${\delta}$ mutant, but not Go6976(classical PKC- ${\alpha}$ inhibitor) and myristoylated PKC- ${\xi}$ inhibitor, attenuated PA-induced ICAM-1 expression. The p38 mitogen-activated protein kinase(MAPK) inhibitor blocked PA-induced ICAM-1 expression in contrast, ERK upstream inhibitor didn't block ICAM-1. Conclusion: These data suggest that PA-induced ICAM-1 expression and cell-cell adhesion in macrophages requires PKC- ${\delta}$ activation and that PKC- ${\delta}$ activation is triggers to sequential activation of p38 MAPK.

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  6. [국내논문]   다양한 NSAID가 인간 치주인대세포의 prostaglandin 합성 및 세포 형태에 미치는 효과에 대한 연구  

    김혁수 (서울대학교 치과대학 교정학교실 ) , 심혜영 (서울특별시립 보라매병원 치과 ) , 채창훈 (나노큐어텍 나노-바이오 퓨전 부설연구소 ) , 장영일 (서울대학교 치과대학 교정학교실 ) , 박준우 (한림대학교 의과대학 치과학교실)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 455 - 463 , 2007 , 1225-1585 ,

    초록

    The present study was designed to evaluate effects of the commonly used NSAIDs(acetaminophen, aspirin, and ibuprofen) on human periodontal ligament cells. Human periodontal ligament cells were grown from a cell line provided by Kyungpook National University. Effects of NSAIDs on the proliferation of human periodontal ligament cells were assessed using MTT assays. And then $PGE_2$ concentrations were determined by ELISA and the changes of cellular configuration were found by electron micrograph. The results were as follows; 1. The MTT assay demonstrated that the commonly used NSAIDs(acetaminophen, aspirin, and ibuprofen) had not significant cytotoxic effect on human periodontal ligament cells. 2. NSAIDs inhibited the $PGE_2$ synthesis of human periodontal ligament cells compared with the control group. These inhibitory effects had no significant differences with NSAID type and concentration. 3. Electron micrographs of human periodontal ligament cells treated with NSAIDs showed more narrow and irregular shape.

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  7. [국내논문]   가토 두개골 결손부에 이식된 새로운 합성 골이식재의 골치유능력   피인용횟수: 1

    노규섭 (단국대학교 치과대학 구강악안면외과학교실 ) , 한세진 (단국대학교 치과대학 구강악안면외과학교실 ) , 김철환 (단국대학교 치과대학 구강악안면외과학교실 ) , 김경욱 (단국대학교 치과대학 구강악안면외과학교실)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 464 - 469 , 2007 , 1225-1585 ,

    초록

    The bone graft materials are grossly divided into autogenous bone, allogenic bone, xenogenic bone, and alloplastic material. Among the various allogenic graft materials, hydroxyapatite( $Ca_{10}(PO_4)_6(OH)_2$ , HA), the main inorganic phase of human hard tissue, is widely used as a repair material for bones. When HA applied to bony defect, however, it may be encapsulated with fibrous tissue and floated in the implanted area by the lack of consolidation. Fluoridated hydroxyapatite( $Ca_{10}(PO_4)_6(OH)_2$ , FHA), where F- partially replaces the OH- in the hydroxyapatite, is considered as an alternative material for bone repair due to its solubility and biocompatibility. This study was designed to find out the bone healing capacity of FHA newly produced as a nanoscale fiber in the laboratory. We implanted HA and FHA in the rabbit cranium defect and histologically analysed the specimen. The results were as follows. 1. In the 4 weeks, fibrous connective tissue and little bone formation around materials of the experimental group I implanted HA were observed. In the experimental group II implanted FHA, newly formed bone around materials were observed. 2. In the 8 weeks, the amount of newly formed and matured bone of the experimental group II was more than the experimental group I and control group. From the results obtained, we suggest that FHA, newly synthesized, is relatively favorable bone substitute with bioconpatibility and has better bone healing capacity than pure HA.

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  8. [국내논문]   하악골 신장술에서 압축자극을 통한 골 재생방식에 대한 생체 역학적 평가  

    허준 (부산대학교 치과대학 구강악안면외과학교실 ) , 김욱규 (부산대학교 치과대학 구강악안면외과학교실 ) , 황대석 (부산대학교 치과대학 구강악안면외과학교실 ) , 김용덕 (부산대학교 치과대학 구강악안면외과학교실 ) , 신상훈 (부산대학교 치과대학 구강악안면외과학교실 ) , 정인교 (부산대학교 치과대학 구강악안면외과학교실 ) , 김철훈 (동아대학교병원 구강악안면외과 ) , 윤석영 (부산대학교 공과대학)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 470 - 478 , 2007 , 1225-1585 ,

    초록

    The purpose of this study was to investigate the clinical, biomechanical, and histologic changes in new distraction osteogenesis(DO) technique combined with a compression stimulation in accordance to different compression-distraction force ratio. 23 adult male rabbits underwent open-osteotomy at the mandibular body area and a external distraction device was applied. In the control group of 8 rabbits, only a 8 mm of distraction was performed by conventional DO technique. In an experimental group of 15 rabbits, a distraction followed by a compression force was performed according to the ratio of compression-distraction suggested by authors. The rate of experimental group I was set up as a 2 mm compression versus 10 mm distraction and the rate of experimental group II was set up as a 3 mm compression versus 11 mm distraction. All the rabbits were sacrificed for a gross finding, biomechanical, histomorphometric and histologic findings at the time of 55 days from the operation day. The results were as follows: 1. On the gross findings, because all rabbits had a sufficient healing time, every distracted new bone had good bone quality and we could not find any difference among all three groups. 2. In the histologic findings, rapid bone maturation(wide lamellar bone formation in the cancellous and cortical bone areas) was observed in two experimental groups compared to the control group. 3. On the bone density tests, the experimental group II showed higher bone density than the other experimental group and control group(control group- $0,2906g/cm^2$ , experimental group I- $0.2961g/cm^2$ , experimental group II- $0.3328g/cm^2$ ). 4. On the biomechanical tests, the experimental group II had significantly higher bone microhardness than the other experimental group and control group(control group-252.7 MPa, experimental group I-263.5 MPa, experimental group II-426.0 MPa). 5. On the microhardness tests, when we compared the hardness ratio of distracted bone versus normal bone, we could find experimental group II had significantly higher hardness ratio than the other experimental group and control group(control group-0.47, experimental group I-0.575, experimental group II-0.80). From this study, we could deduce that the modified distraction osteogenesis method with a compression stimulation might improve the quality of bone regeneration and shorten the consolidation period in comparison with conventional distraction osteogenesis techniques.

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  9. [국내논문]   다형성 선종에서 발생한 구개부의 상피성-근상피암종   피인용횟수: 1

    김경욱 (단국대학교 치과대학 구강악안면외과학교실 ) , 한세진 (단국대학교 치과대학 구강악안면외과학교실)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 479 - 484 , 2007 , 1225-1585 ,

    초록

    본 교실에서는 상악 구개부의 무통성 종창을 주소로 내원한 다형성 선종의 병력을 가진 환자에 대한 임상적, 조직병리학적, 면역병리학적 검사들을 통하여 최종적으로, 드물게 발병하는 다형성 선종 유래 악성 상피성-근상피암종으로 진단하였으며, 상악골 부분 절제술 및 측두근 피판 지연 재건술 시행 후 현재까지 재발의 소견 없이 양호한 치료 결과를 얻었기에 보고하는 바이다.

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  10. [국내논문]   제 3 대구치 발치시 환자의 호흡 패턴이 스트레스 변화 양상에 미치는 영향  

    이상명 (한림대학교 전자공학과 의공학연구실 ) , 김형욱 (한림대학교 임상치의학 대학원 ) , 박양호 (한림대학교 임상치의학 대학원 ) , 김진철 (한림대학교 임상치의학 대학원 ) , 박준우 (한림대학교 임상치의학 대학원 ) , 이성준 (한림대학교 전자공학과 의공학연구실 ) , (한림대학교 전자공학과 의공학연구실 ) , 안재목 (한림대학교 전자공학과 의공학연구실)
    大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons v.33 no.5 ,pp. 485 - 493 , 2007 , 1225-1585 ,

    초록

    Purpose: Heart rate variability(HRV) is the clinical consequence of various influences of the autonomic nervous system(ANS) on heart beat. HRV can estimate the potential physiologic rhythm from the interval between consecutive beats(RR interval or HRV data). It is known as RSA which represents respiration-related HR rhythmic oscillation. Previous studies demonstrated a specific breathing pattern(0.1Hz, 6breaths/min) to improve a physiological body condition related to the stress. In this paper, the level of stress would be evaluated in terms of three phases of the dental treatment, combined with 6breaths/min. Methods: These phases include before, during and after tooth extraction or anesthesia or something.36 patients' stresses were assessed using HRV stress analyzer in each phase in Kangdong Sacred Heart Hospital, and Chuncheon Sacred Heart Hospital, Hallym University Medical Center from Jun. to Sept. of 2007. HRV 5-min data collected were analyzed in time-domain and frequency-domain to evaluate the activity of autonomic nervous system(ANS) which represents the level of stress. Results: All HRV parameters including HF(high frequency), LF(low frequency) and LF/HF ratio showned a significant change affecting the ANS balance. There was a 6.4% difference between R(LF/HF)s on general breathing pattern for balance of Autonomic nervous system, but on controlled breathing pattern, 0.1Hz, was made narrow till 1.4%. The activity of ANS has increased by 1.4% on general breathing pattern, and by 2.9% on controlled breathing pattern, 0.1Hz. Conclusion: After analysis of preoperative stress changes and effect of breathing pattern of 0.1 Hz on the stress in 36 patients who have undergone third molar extraction, following was concluded. In the preoperative stage, the sympathetic change was the greatest?after the?anesthetic injection, and stress was relieved by controlling the breathing pattern to a frequency of 0.1Hz.

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