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Molecular & cellular toxicology 15건

  1. [국내논문]   Up-regulation of cancer-related genes in HepG2 cells by TCDD requires PRMT I and IV   SCIE

    Lee, Joo-Hyun (Postgraduate Studies of Public Health, Graduate School, Korea University ) , Lee, Eun-Il (Postgraduate Studies of Public Health, Graduate School, Korea University ) , Kwon, Dae-Ho (Department of Preventive Medicine and Medical Research Center for Environmental Toxico-Genomics and Proteomics, Korea University College of Medicine ) , Lim, Yong-Chul (Department of Preventive Medicine and Medical Research Center for Environmental Toxico-Genomics and Proteomics, Korea University College of Medicine ) , Oh, Sang-Nam (Department of Preventive Medicine and Medical Research Center for Environmental Toxico-Genomics and Proteomics, Korea University College of Medicine ) , Oh, Min-Yeong (Postgraduate Studies of Public Health, Graduate School, Korea University ) , Hong, Eun-Young (Postgraduate Studies of Public Health, Graduate School, Korea University)
    Molecular & cellular toxicology v.6 no.2 ,pp. 111 - 118 , 2010 , 1738-642x ,

    초록

    The 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a well-known carcinogen, however, the biological mechanism of carcinogenesis by TCDD has not been established. Recently, protein arginine methyltransferases (PRMTs) have been identified as secondary transcription co-activators and are proposed to be co-activators of aryl hydrocarbon receptors binding to xenobiotic response elements. Both PRMT1 and PRMT4 were also reported to be involved with carcinogenesis. The aim of this study was to identify cancer-related genes that are regulated by TCDD exposure and the effect of arginine methylation on TCDD toxicity by transfecting human hepatocarcinoma cells with PRMT1 and PRMT4 siRNA. By micro-array analysis, 1,461 genes were up-regulated and 1,591 genes were down-regulated by TCDD exposure. Among the 16 up-regulated genes which had functions related to cancer or metastasis, 13 genes were confirmed by quantitative real time RT-PCR: ABCG2, NRP1, SOX5, BIRC3, CD109, CYP1A1, ERBB2, MTA1, FURIN, F3, PIK3R3, NPTN and NTN4. Co-inhibition of PRMT1 and PRMT4 resulted in decreased expression of eight of these genes, MTA1, ERBB2, SOX5, CD109, FURIN, NRP1, PIK3R3 and ABCG2, all of which have been reportedly involved in breast, ovary, prostate and lung cancers, and metastasis.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  2. [국내논문]   Cytotoxicity and genotoxicity of nano-silver in mammalian cell lines   SCIE

    Kim, Youn-Jung (Department of Applied Chemistry, Kyung Hee University ) , Yang, Sung-Ik (Department of Applied Chemistry, Kyung Hee University ) , Ryu, Jae-Chun (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science & Technology)
    Molecular & cellular toxicology v.6 no.2 ,pp. 119 - 125 , 2010 , 1738-642x ,

    초록

    Nano-silver (Ag) with antimicrobial activity is by far the most commercialized nano-compound. The hazards associated with human exposure to nano-sized-silver should be investigated to facilitate the risk assessment process. Recent studies have shown that inflammatory, oxidative, genotoxic, and cytotoxic consequences are associated with silver particulate exposure, and are inherently linked. In the present study, the cytotoxicity and genotoxicity of nano-silver were investigated using the dye exclusion assay, the comet assay, and the mouse lymphoma thymidine kinase ( $tk^{+/-}$ ) gene mutation assay (MLA). $IC_{20}$ values of nano-silver in L5178Y cells were determined the concentration of $3,769.53\;{\mu}g/mL$ and $1,796.88\;{\mu}g/mL$ with and without S-9, respectively. And in BEAS-2B cell, $IC_{20}$ values were calculated to $1,171.88\;{\mu}g/mL$ and $761.72\;{\mu}g/mL$ with and without S-9, respectively. From these results, nano-silver was more cytotoxic in absence of S-9 metabolic activation system and at the BEAS-2B cells. In the comet assay, L5178Y cells and BEAS-2B cells were treated with nano-silver which significantly increased > 2-fold tail moment with and without S-9. However, the mutant frequencies in the nano-silver treated L5178Y cells were slightly increased but not significant compared to the vehicle controls with and without S-9. The results of this study indicate that nano-silver can cause primary DNA damage and cytotoxicity but not mutagenicity in cultured mammalian cells.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [국내논문]   Heme oxygenase-1 mediated protective effect of methyl gallate on cadmium-induced cytotoxicity in cultured mouse mesangial cells   SCIE

    Cha, Seok-Ho (Department of Medical Biotechnology, College of Medicine, Inha University ) , Suh, Chang-Kook (Department of Physiology and Biophysics, College of Medicine, Inha University)
    Molecular & cellular toxicology v.6 no.2 ,pp. 127 - 133 , 2010 , 1738-642x ,

    초록

    To clarify the effect of a phytochemical, methyl gallate (MG) on a heavy metal (cadmium)-induced renal toxicity, cytotoxicity and the change of heme oxygenase-1 (HO-1) gene expression was studied using cultured mouse renal glomerular mesangial cells (MMC). By employing RT-PCR and Western blotting analysis, we have examined the HO-1 induction in MMCs that were treated with $Cd^{2+}$ and/or MG. Using MTT assay we have also examined the cytoprotective effect of HO-1 induction against the cytotoxicity caused by toxic dose of $Cd^{2+}$ . In MMCs exposed to $Cd^{2+}$ and MG, expression of HO-1 (mRNA and protein) was increased in a concentration- and time-dependent manner. The increments of HO-1 mRNA and protein expressions by $Cd^{2+}$ and MG were inhibited by the treatment of the cells with actinomycin D, an inhibitor of transcription. The decreased viability of the cells by $Cd^{2+}$ was partially recovered by the treatment of MG and this recovery by the MG was reduced by the treatment of zinc protoporphyrin IX (a HO-1 inhibitor). From these results, methyl gallate might have cytoprotective effect on $Cd^{2+}$ -induced cytotoxicity that is related with heme oxygenase-1 induction.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  4. [국내논문]   Antidiabetic and renoprotective effects of Corni Fructus extract in db/db mice   SCIE

    Kim, Hye-Jeong (Department of Public Health, Keimyung University, ) , Kim, Young-Chul (Department of Public Health, Keimyung University,)
    Molecular & cellular toxicology v.6 no.2 ,pp. 135 - 142 , 2010 , 1738-642x ,

    초록

    This study investigated the effect of Corni Fructus (Cornus officinalis Sieb. et Zucc.) extract on hyperglycemia, renal dysfunction and diabetic nephropathy in db/db mice. Eight weeks old male mice were divided into normal control group (NC), diabetic control group (DC) and Corni Fructus treated diabetic group (DCF). Over an 8-week experimental period, Corni Fructus extract was administered orally at 500 mg/kg BW/day. Final fasting serum glucose level in the DCF group was significantly lower than the DC group by 32% (P $\beta_1$ in kidneys were significantly lower (P $\beta_1$ in kidneys of the DCF group was more mildly expressed than the DC group. In conclusion, these results indicate that Corni Fructus can be effective on hyperglycemia, renal dysfunction and diabetic nephropathy in db/db mice.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [국내논문]   Systemic immunity of obese-diabetes model (db/db) mice   SCIE 피인용횟수: 1

    Lee, Sang-Eun (Clinical Research Institute, Samsung Medical Center Sungkyunkwan University School of Medicine ) , Jang, Ik-Soon (Korea Basic Science Institute ) , Park, Jun-Soo (Division of Biological Sciences and Technology, Yonsei University ) , Lee, Ji-Hae (Department of Life Science, Yong-In University ) , Lee, Seung-Yeul (Department of Life Science, Yong-In University ) , Baek, So-Young (Clinical Research Institute, Samsung Medical Center Sungkyunkwan University School of Medicine ) , Lee, Seung-Hoon (Department of Life Science, Yong-In University ) , Lee, Hyun-Ah (Clinical Research Institute, Samsung Medical Center Sungkyunkwan University School of Medicine)
    Molecular & cellular toxicology v.6 no.2 ,pp. 143 - 149 , 2010 , 1738-642x ,

    초록

    Obesity has recently been defined as a chronic inflammatory disease and is considered as a major cause of adult health problems including incurable diseases like diabetes and cancer. In this study, the systemic immunity, including the innate and adaptive immunity parameters, of naturally occurring (leptin receptor mutation) obesity-diabetes mice (db/db) was examined to increase our knowledge of these mice for obesity-related studies. Severe fatty liver with blood engorgement was observed in the db/db mice. Compared to background C57BL/6J mice, the adaptive immunity, as measured by mitogen-induced T and B cell proliferation and cytokine release, was significantly suppressed in db/db mice. However, significant upregulation of innate immune-inflammatory parameters including macrophage function and NK cell activity was observed in db/db mice without external stimulation. These data conclusively confirm the systemic immune-inflammatory micro-environment with suppressed adaptive immunity of db/db mice, which may cause the secondary obesity-related life-threatening diseases like diabetes or cancer.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [국내논문]   Influence of quercetin on the physiological response to cadmium stress in olive flounder, Paralichthys olivaceus: effects on hematological and biochemical parameters   SCIE

    Park, Mi-Seon (East Sea Fisheries Research Institute, National Fisheries Research & Development Institute ) , Shin, Hyun-Suk (Division of Marine Environment & BioScience, Korea Maritime University ) , Lee, Je-Hee (Department of Marine Life Sciences, Jeju National University ) , Kil, Gyung-Suk (Division of Electrical & Electronic Engineering, Korea Maritime University ) , Choi, Cheol-Young (Division of Marine Environment & BioScience, Korea Maritime University)
    Molecular & cellular toxicology v.6 no.2 ,pp. 151 - 159 , 2010 , 1738-642x ,

    초록

    The aim of the present study was to determine whether quercetin, an antioxidant and radical scavenger as natural flavonoid, would be able to offer any protection against cadmium (Cd) toxicity in olive flounder, Paralichthys olivaceus with emphasis on biochemical analysis. Fish were pre-treated with 0% (Diet 1), 0.25% (Diet 2) and 0.5% (Diet 3) quercetin for 30 and 60 day and after that, fish were post-exposed to 10 ppb Cd for 0, 6, 12, 24, and 48 hr. To understand the stress-resistance effect of quercetin, we measured the mRNA expression of metallothionein (MT), glucocorticoid receptor (GR), and level of acetylcholinesterase (AChE) in quercetin-treated flounder exposed to Cd. The MT and GR expression levels were lower in flounder fed Diets 2 and 3 than in those fed Diet 1, and AChE level was higher in flounder fed Diet 2 and 3 than in those fed Diet 1. Plasma cortisol increased in fish fed Diets 1, 2, and 3, but it was lower in fish fed Diets 2 and 3 than in those fed Diet 1. In addition, lipid peroxidation (LPO) levels lower than with Diet 1, which protected the cell membrane. We also investigated the effects of cortisol on stress resistance in vitro. Results showed that the MT and GR expression levels were lower in livers of flounder fed with Diets 2 and 3 than those fed with Diet 1, suggesting that quercetin reduced the stress induced by Cd. These results indicate that quercetin has a stress-resistance effect and acts to maintain physiological homeostasis.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [국내논문]   TNF-α and TNF-β polymorphisms with susceptibility to gastric cancer in a Korean population   SCIE

    Yoon, Jung Hwan , Song, Jae Hwi , Kang, Young Hwi , Park, Yong Kyu , Zhang, Cao , Nam, Suk Woo , Lee, Jung Young , Park, Won Sang
    Molecular & cellular toxicology v.6 no.2 ,pp. 161 - 167 , 2010 , 1738-642x ,

    초록

    The aim of the present study was to determine whether quercetin, an antioxidant and radical scavenger as natural flavonoid, would be able to offer any protection against cadmium (Cd) toxicity in olive flounder, Paralichthys olivaceus with emphasis on biochemical analysis. Fish were pre-treated with 0% (Diet 1), 0.25% (Diet 2) and 0.5% (Diet 3) quercetin for 30 and 60 day and after that, fish were post-exposed to 10 ppb Cd for 0, 6, 12, 24, and 48 hr. To understand the stress-resistance effect of quercetin, we measured the mRNA expression of metallothionein (MT), glucocorticoid receptor (GR), and level of acetylcholinesterase (AChE) in quercetin-treated flounder exposed to Cd. The MT and GR expression levels were lower in flounder fed Diets 2 and 3 than in those fed Diet 1, and AChE level was higher in flounder fed Diet 2 and 3 than in those fed Diet 1. Plasma cortisol increased in fish fed Diets 1, 2, and 3, but it was lower in fish fed Diets 2 and 3 than in those fed Diet 1. In addition, lipid peroxidation (LPO) levels lower than with Diet 1, which protected the cell membrane. We also investigated the effects of cortisol on stress resistance in vitro. Results showed that the MT and GR expression levels were lower in livers of flounder fed with Diets 2 and 3 than those fed with Diet 1, suggesting that quercetin reduced the stress induced by Cd. These results indicate that quercetin has a stress-resistance effect and acts to maintain physiological homeostasis.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  8. [국내논문]   TNF-α and TNF-β polymorphisms with susceptibility to gastric cancer in a Korean population   SCIE

    Yoon, Jung Hwan , Song, Jae Hwi , Kang, Young Hwi , Park, Yong Kyu , Zhang, Cao , Nam, Suk Woo , Lee, Jung Young , Park, Won Sang
    Molecular & cellular toxicology v.6 no.2 ,pp. 161 - 167 , 2010 , 1738-642x ,

    초록

    The aim of the present study was to determine whether quercetin, an antioxidant and radical scavenger as natural flavonoid, would be able to offer any protection against cadmium (Cd) toxicity in olive flounder, Paralichthys olivaceus with emphasis on biochemical analysis. Fish were pre-treated with 0% (Diet 1), 0.25% (Diet 2) and 0.5% (Diet 3) quercetin for 30 and 60 day and after that, fish were post-exposed to 10 ppb Cd for 0, 6, 12, 24, and 48 hr. To understand the stress-resistance effect of quercetin, we measured the mRNA expression of metallothionein (MT), glucocorticoid receptor (GR), and level of acetylcholinesterase (AChE) in quercetin-treated flounder exposed to Cd. The MT and GR expression levels were lower in flounder fed Diets 2 and 3 than in those fed Diet 1, and AChE level was higher in flounder fed Diet 2 and 3 than in those fed Diet 1. Plasma cortisol increased in fish fed Diets 1, 2, and 3, but it was lower in fish fed Diets 2 and 3 than in those fed Diet 1. In addition, lipid peroxidation (LPO) levels lower than with Diet 1, which protected the cell membrane. We also investigated the effects of cortisol on stress resistance in vitro. Results showed that the MT and GR expression levels were lower in livers of flounder fed with Diets 2 and 3 than those fed with Diet 1, suggesting that quercetin reduced the stress induced by Cd. These results indicate that quercetin has a stress-resistance effect and acts to maintain physiological homeostasis.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  9. [국내논문]   TNF-$\alpha$ and TNF-$\beta$ polymorphisms with susceptibility to gastric cancer in a Korean population   SCIE

    Yoon, Jung-Hwan ; Song, Jae-Hwi ; Kang, Young-Hwi ; Park, Yong-Kyu ; Cao, Zhang ; Nam, Suk-Woo ; Lee, Jung-Young ; Park, Won-Sang
    Molecular & cellular toxicology v.6 no.2 ,pp. 161 - 167 , 2010 , 1738-642x ,

    초록

    Tumor necrosis factors (TNF) regulate the inflammatory response, cell growth and differentiation and its genetic polymorphisms are correlated with various cancer types including colon, and ovarian cancer. To determine whether polymorphisms at TNFA -G308A and TNFB +G252A were associated with a susceptibility to gastric cancer, we investigated the genotype and allele frequencies of these genes in 181 gastric cancer patients and 290 healthy individuals. The polymorphism analysis was performed by amplifying the promoter region of TNF- $\alpha$ for TNFA -308 and the first intron of TNF- $\beta$ for TNFB +252, digestion with NCOl restriction enzymes, and then sequencing the products. The frequencies of the genotypes for TNFA -308 were G/G, G/A, and A/A, 93.4% (169/181), 5.0% (9/181) and 1.7% (3/181), respectively, in gastric cancer patients and 88.6% (257/290), 11.4% (33/290) and 0% (0/290), respectively, in the healthy controls. Statistically, there was a significant difference in the genotype frequency of the TNFA -308 between the healthy controls and the gastric cancer patients (P=0.0034). However, genotype and allele frequency for TNFB +252 was not associated with an increased risk of gastric cancer in this population. When stratified by histological subtype of gastric cancer, there was no statistical significance between the risk and the two polymorphisms of TNFA -308 and TNFB +252. Our findings suggest that the TNFA -308 polymorphism of the TNF- $\alpha$ gene may be closely associated with susceptibility to gastric cancer in Korean patients.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  10. [국내논문]   Changes of miRNA and mRNA expression in HepG2 cells treated by epigallocatechin gallate   SCIE 피인용횟수: 1

    Ahn, Joon-Ik (Pharmacological Research Division, Toxicological Evaluation Research Department. National Institute of Food and Drug Safety Evaluation ) , Jeong, Kyung-Ji (Pharmacological Research Division, Toxicological Evaluation Research Department. National Institute of Food and Drug Safety Evaluation ) , Ko, Moon-Jeong (Pharmacological Research Division, Toxicological Evaluation Research Department. National Institute of Food and Drug Safety Evaluation ) , Shin, Hee-Jung (Pharmacological Research Division, Toxicological Evaluation Research Department. National Institute of Food and Drug Safety Evaluation ) , Kim, Hye-Soo (Pharmacological Research Division, Toxicological Evaluation Research Department. National Institute of Food and Drug Safety Evaluation ) , Chung, Hye-Joo (Pharmacological Research Division, Toxicological Evaluation Research Department. National Institute of Food and Drug Safety Evaluation ) , Jeong, Ho-Sang (Pharmacological Research Division, Toxicological Evaluation Research Department. National Institute of Food and Drug Safety Evaluation)
    Molecular & cellular toxicology v.6 no.2 ,pp. 169 - 177 , 2010 , 1738-642x ,

    초록

    Epigallocatechin gallate (EGCG), a major type of green tea polyphenol, has been reported to cause hepatotoxicity when used in excess. Micro-RNAs (miRNAs) are small non-coding RNA molecules that functions as negative regulators of gene expression. They play an important role in the regulation of genes involved in the cellular and molecular responses to toxicants. To examine miRNA regulatory effect on global gene expression in EGCG-treated HepG2 cells, we performed pair-wise correlation coefficient analysis on expression levels of 22 miRNA and 27,419 mRNA, and observed negative correlations (r

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