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Journal of crop science and biotechnology 9건

  1. [국내논문]   Genetic Variation in Mutants of Black Gram (Vigna mungo (L.) Hepper) Evaluated by RAPD Markers  

    Arulbalachandran, D. (Department of Botany, Annamalai University ) , Mullainathan, L. (Department of Botany, Annamalai University ) , Karthigayan, S. (CAS in Marine Biology, Annamalai University ) , Somasundaram, S.T. (CAS in Marine Biology, Annamalai University ) , Velu, S. (Department of Botany, Annamalai University)
    Journal of crop science and biotechnology v.13 no.1 ,pp. 1 - 6 , 2010 , 1975-9479 ,

    초록

    Genetic variation among four mutants with parent of black gram (high seed protein, tall, bushy, and dwarf mutants) was investigated. The mutants were generated with physical gamma rays and chemical ethyl methane sulphonate. These mutants had some advantages like high protein content, lodging resistance, and high seed yield compared with parent cultivars (cvs.). The objective of the present investigation is to find genetic variation in mutants of black gram with their parents. The genetic variation was evaluated with 20 random primers, generated total 202 fragments scored with 58 polymorphic alleles, and the average was 10.1 alleles per locus and a range of 1-9 alleles. The average polymorphic rates were 38.37 among the mutants and parents through the 20 primers. Primers OPA-14 and OPI-04 revealed 35% of DNA polymorphism in this investigation. The genetic distance (GD) among the genotypes was 0.19 suggesting a significant degree of genetic diversity. The five genotypes were used to construct a dendrogram based on the similarity matrix, revealing a genetic distance varying from 0.600 to 0.725. This variation was due to the mutation induced by gamma rays and ethyl methane sulphonate. Further research is needed to analyze the sequence and expression of these mutated genes and to develop and improve black gram via genetic engineering to evaluate their use.

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  2. [국내논문]   Fine Mapping of Grain Weight QTLs Using Near Isogenic Lines from a Cross between Oryza sativa and O. grandiglumis  

    Oh, Ji-Min (Department of Agronomy, Chungnam National University ) , Yoon, Dong-Beom (Department of Agronomy, Chungnam National University ) , Ahn, Sang-Nag (Department of Agronomy, Chungnam National University)
    Journal of crop science and biotechnology v.13 no.1 ,pp. 7 - 12 , 2010 , 1975-9479 ,

    초록

    In a previous study, we reported the grain weight QTL, tgw2 in the 150 $F_{2:3}$ lines derived from a cross between Oryza sativa subssp. Japonica cv. Hwaseongbyeo and HG101. This QTL was confirmed in $F_4$ lines (CR1242) segregating for the target region. For fine mapping of tgw2, one $F_5$ plant homozygous for the O. grandiglumis DNA in the target region was selected from CR1242 and crossed with Hwaseongbyeo to produce the $F_2$ and $F_3$ populations. QTL analysis using 490 $F_2$ plants confirmed the existence of tgw2 with an $R^2$ value of 28.0%. This QTL explained 61.3% of the phenotypic variance for 1,000-grain weight in 64 $F_3$ lines. Substitution mapping with 47 $F_3$ lines and 74 $F_4$ plants with informative recombination breakpoints in the target region was carried out to narrow down the position of the tgw2. The result indicated that tgw2 was located in the 384-kb interval between two SSR markers, RM12813 and RM12836. Annotation data of BACs in this 384-kb region revealed that forty-five putative genes exist in this interval including the GW2 gene responsible for grain weight and width. Considering the position of the QTL tgw2, it appears that tgw2 is functionally related to the gene GW2. However, the possibility that another unknown mechanism might be responsible for regulation of grain weight at tgw2 cannot be ruled out. Four QTLs for grain length, grain width, and grain thickness were also located in the same interval suggesting that a single gene is involved in controlling these four traits. Substitution mapping also indicated that two QTLs for grain weight and culm length, tgw2 and cl2, were tightly linked.

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  3. [국내논문]   High Frequency Direct Plant Regeneration, Micropropagation and Shikonin Induction in Arnebia hispidissima   피인용횟수: 1

    Pal, Minakshi (Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology ) , Chaudhury, Ashok (Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology)
    Journal of crop science and biotechnology v.13 no.1 ,pp. 13 - 19 , 2010 , 1975-9479 ,

    초록

    The data presented herein reports a rapid and efficient method for direct plant regeneration at high frequency without intervening callus formation from shoot tip (93%) and nodal segment (60%) cultured on MS media supplemented with 0.5 mg $1^{-1}$ KIN, 0.25 mg $1^{-1}$ BAP, 0.1 mg $1^{-1}$ IAA and 100 mg $1^{-1}$ CH. Conversely, leaf and internodal explants were poorly responsive. Adventitious shoot buds arose not only from the cut ends but all along the surface of the explants leading to the formation of clusters with multiple shoots. Multiple shoots upon transfer to MS media supplemented with 2.0 mg $1^{-1}$ IBA induced efficient rooting (80%). In vitro flowering was observed when tissue culture-raised plantlets were maintained for extended period in culture. Shikonin was induced in roots of regenerated plants which often exudates in the culture medium was quantified spectrophotometerically by recording absorbance at 620 nm and estimated to be 0.50 mg $g^{-1}$ fresh weight of tissue at the end of the 50 days of culture. The regenerated plants were successfully acclimatized, hardened, and transferred to soil in green house for micropropagation. The protocol developed here will be very useful for the supply of Arnebia hispidissima all year as a raw product necessary for obtaining Shikonin for the cosmetic, dyeing, food, and pharmaceutical industries.

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  4. [국내논문]   Comparative Study on Growth and Development of Some Accessions of Local Germplasm of Bambara Groundnut (Vigna subterrenea L. Verdc.) of Nigeria in Two Cropping Seasons  

    Chijioke, Oyiga Benedict (Department of Crop Science, University of Nigeria ) , Ifeanyi, Uguru Michael (Department of Crop Science, University of Nigeria ) , Blessing, Aruah Chineny (Department of Crop Science, University of Nigeria)
    Journal of crop science and biotechnology v.13 no.1 ,pp. 21 - 27 , 2010 , 1975-9479 ,

    초록

    Two field experiments were conducted in April (early cropping) and August, (late cropping) 2007 at the Department of Crop Science Research Farm, University of Nigeria, Nsukka, to evaluate the agronomic and yield attributes of 13 cultivars of bambara groundnut. The results obtained showed that planting dates had significant effects (P

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  5. [국내논문]   Effect of Combined Indole Acetic Acid and Mepiquat Chloride on Endogenous Gibberellins and Tuber Growth in Chinese Yam (Dioscorea opposita Thunb.)   피인용횟수: 1

    Kim, Sang-Kuk (Division of Crop Science, Gyeongbuk Agricultural Technology Administration ) , Choi, Hong-Jib (Division of Crop Science, Gyeongbuk Agricultural Technology Administration ) , Lee, In-Jung (Division of Plant Biosciences, College of Agriculture and Life Science, Kyungpook National University ) , Kim, Hak-Yoon (Division of Environmental Science & Engineering, Keimyung University)
    Journal of crop science and biotechnology v.13 no.1 ,pp. 29 - 32 , 2010 , 1975-9479 ,

    초록

    The combined effect of indole-3-acetic acid and mepiquat chloride on the endogenous gibberellins of aerial and underground tubers as well as tuber yield was studied on Chinese yam plant. Two plant hormones were combined in the following concentrations: IAA 50, 100, 200, and 300 ppm, plus MC 600 ppm. Combined plant hormones were foliar-sprayed once in early July. Exogenously applied IAA combining mepiquat chloride increased fresh weight of the aerial and underground tubers affecting the increase of endogenous gibberellins. The results suggest that IAA is likely to be one of the active plant hormones responsible for aerial tuber growth and enlargement, stimulating the promotion of endogenous gibberellin when combined with mepiquat chloride.

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  6. [국내논문]   Assessment of Genetic Divergence in Salt Tolerance of Soybean (Glycine max L.) Genotypes  

    Mannan, M.A. (Department of Agronomy, Patuakhali Science and Technology University ) , Karim, M.A. (Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University ) , Khaliq, Q.A. (Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University ) , Haque, M.M. (Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University ) , Mian, M.A.K. (Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University ) , Ahmed, J.U. (Department of Crop Botany, Bangabandhu Sheikh Mujibur Rahman Agricultural University)
    Journal of crop science and biotechnology v.13 no.1 ,pp. 33 - 37 , 2010 , 1975-9479 ,

    초록

    A large number of soybean (Glycine max L.) genotypes of diverse growth habit and adaptive characters were used in the experiment. Soil salinity-induced changes in nine morpho-physiological characters of 30-day-old seedlings of 170 soybean genotypes were compared in the study. The first and second principal components (PC) of principal component analysis (PCA) results accounted for 97 and 2.5%, respectively, of the total variations of soybean genotypes. The variation for the first PC was composed mainly of relative total dry weight (DW), relative shoot dry weight, as well as petiole dry weight. There were four clusters distinguished in the cluster analysis. The genotypes in cluster IV performed better in respect to relative total dry weight and relative shoot dry weight and hence having salt tolerance. The genotypes clusters III performed very poorly and those of clusters II and I were moderate to poor. $D^2$ analysis indicated that the clusters differed significantly from each other. Discriminant function analysis (DFA) again asserts strongly that more than 92% of the genotypes were correctly assigned to clusters. Both PCA and DFA confirmed that the relative total DW followed by shoot and petiole DW were the major discriminatory variables, and the root DW were the secondary important variables to distinguish genotypes into groups. In this study, multivariate analyses were used in identifying the soybean genotypes of desirable traits for salt tolerance.

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  7. [국내논문]   Oil Biosynthesis and its Related Variables in Developing Seeds of Mustard (Brassica juncea L.) as Influenced by Sulphur Fertilization  

    Fazili, Inayat S. (Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Hamdard University ) , Masoodi, Muzain (Department of Horticulture, SKUAST(K) ) , Ahmad, Saif (Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Hamdard University ) , Jamal, Arshad (Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Hamdard University ) , Khan, Jafar S. (Council of Scientific and Industrial Research (CSIR) ) , Abdin, Malik Z. (Centre for Transgenic Plant Development, Department of Biotechnology, Faculty of Science, Hamdard University)
    Journal of crop science and biotechnology v.13 no.1 ,pp. 39 - 46 , 2010 , 1975-9479 ,

    초록

    The aim of this research was to study the effect of sulphur (S) fertilization on oil biosynthesis and its related variables at various stages of seed development, and to find possible explanations for increased oil content in the seeds of mustard (Brassica juncea L. Czern and Coss) due to S fertilization. Acetyl-CoA carboxylase activity and contents of oil, acetyl-CoA, soluble protein, total RNA, total sugar and sulphur were determined in the developing seeds of mustard grown in the field with sulphur (+S) and without sulphur (-S). The period between 10 to 30 days after flowering was observed as the active period of oil accumulation in the developing seeds of mustard. The accumulation of the oil was preceded by a marked rise in acetyl-CoA carboxylase activity and acetyl-CoA concentration, which declined rapidly when oil accumulation reached a plateau. Total sugar content decreased, while protein content increased during the active period of oil accumulation in the developing seeds (i.e. between 10-30 days after flowering). Sulphur fertilization significantly (P

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  8. [국내논문]   Estimation of Genetic Distance and Its Predictability of $F_1$ Hybrid Performance in Barley  

    Kim, Hong-Sik (International Technology Cooperation Center, Rural Development Administration ) , Kim, Jung-Gon (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration ) , Baek, Seong-Bum (Upland Crop Division, National Institute of Crop Science, Rural Development Administration ) , Hwang, Jong-Jin (Upland Crop Division, National Institute of Crop Science, Rural Development Administration)
    Journal of crop science and biotechnology v.13 no.1 ,pp. 47 - 52 , 2010 , 1975-9479 ,

    초록

    Heterotic performance of hybrids is dependent upon the amount of genetic variability among parents and combining ability of alleles at the loci associated with target traits. This study was carried out to determine if (1) pedigree-based genetic distance could explain molecular levels of genetic diversity, and (2) molecular marker-based genetic distance could predict the degree of hybrid performance in barley. The average value of Random Amplified Polymorphic DNA (RAPD)-based genetic similarity for all 58 genotypes was 0.641. Twenty-two hulless barley and eight malt barley varieties showed low levels of genetic diversity with higher similarity values than the average of all or of the hulled barley variety pool. Coefficients of parentage (COPs) computed for 1,653 pairs of 58 Korean barley varieties ranged from 0 to 0.984 with a mean of 0.048. Correlation between the genealogical and RAPD-based genetic similarity matrices was 0.256 (P

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  9. [국내논문]   Genetic and Chemical Analyses of six Cowpea and two Phaseolus Bean species Differing in Resistance to Weevil Pest   피인용횟수: 2

    Abdel-Sabour, A.G. (Dept. of Genetics, Faculty of Agriculture, Sohag University ) , Obiadalla-Ali, H.A. (Dept. of Horticulture, Faculty of Agriculture, Sohag University ) , AbdelRehim, K.A. (Dept. of Botany, Faculty of Science, Sohag University)
    Journal of crop science and biotechnology v.13 no.1 ,pp. 53 - 60 , 2010 , 1975-9479 ,

    초록

    The objective of this work was to evaluate the genetic variability among six cowpea (Vigna unguiculata) cultivars differing in their resistance to Callosobruchus maculatus (F.) weevil. Two resistant bean cultivars were used to compare between the sensitive, moderate tolerant, and high tolerant cowpea cultivars. The differentiations were performed by using random amplified polymorphic DNA (RAPD) fingerprinting, protein concentration and organic and non-organic components in seed coat. Six polymorphic primers were identified, resulting in different informative bands. Based on polymorphic profiles, three clusters were formed. Clustering was mainly affected by the resistance to weevil pest. The sensitive cowpea cultivars were separated in one group, the moderate tolerant and high tolerant cultivars came in separate groups, and finally, the resistant bean cultivars separated clearly in one distinct group. The most interesting result was represented by concentration of total protein in the seed coat. The protein concentration in the resistant bean cultivars were approximately 50% less than concentration in each of the moderate tolerant and sensitive cultivars of cowpea. Ferric ions were about 25% less than the moderate tolerant and sensitive cultivars. The concentrations of calcium and potassium in seed coats were higher in the resistant beans than in cowpea cultivars. Cobalt was about four times higher in resistant bean than in the sensitive and moderate tolerant cowpea cultivars, which may play a major role in seed resistance to weevil.

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