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International journal of veterinary science and me... 14건

  1. [해외논문]   PCR-RFLP of bone morphogenetic protein 15 (BMP15/FecX) gene as a candidate for prolificacy in sheep  

    Nagdy, Hiam (Department of Animal Reproduction & A.I, National Research Centre, Dokki, Giza, Egypt ) , Mahmoud, Karima Gh.M. (Department of Animal Reproduction & A.I, National Research Centre, Dokki, Giza, Egypt ) , Kandiel, Mohamed M.M. (Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt ) , Helmy, Nermeen A. (Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt ) , Ibrahim, Shawky S. (Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt ) , Nawito, Mahmoud F. (Department of Animal Reproduction & A.I, National Research Centre, Dokki, Giza, Egypt ) , Othman, Othman E. (Cell Biology Department, National Research Centre, Dokki, Giza, Egypt)
    International journal of veterinary science and medicine v.6 suppl. ,pp. S68 - S72 , 2018 , 2314-4599 ,

    초록

    Abstract Bone morphogenetic protein 15 (BMP15/FecX) gene is considered one of the major genes and a candidate marker for the reproduction in farm animals, especially sheep. The present study aimed to detect the genetic polymorphisms of BMP15 gene in sheep using PCR-RFLP technique. In the present study, 115 ewes were assigned into high and low prolificacy categories according to their reproductive history. In high prolific group (n = 20), ewes produced twins more than single births. In the low prolific type (n = 95), the ewes produced single births more than twins. DNA was extracted from blood samples of all ewes, subjected to PCR-RFLP analysis and confirmed by sequence analysis. The PCR products of 356 bp size were cut with HinƒI restriction enzyme. Three digested fragments of 70, 117 and 169 bp were obtained in both types of sheep. All animals were homozygous with CC genotype. In conclusion, the accessible findings did not detect any mutation in FecX gene in sheep, regardless their prolificacy. Therefore, further attempts are necessary to detect other SNP for BMP-15 gene in Egyptian sheep breeds.

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  2. [해외논문]   Evaluation of bull spermatozoa during and after cryopreservation: Structural and ultrastructural insights  

    Khalil, Wael A. (Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt ) , El-Harairy, Mostafa A. (Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt ) , Zeidan, Alaa E.B. (Animal Production Research Institute, Dokki, Giza, Egypt ) , Hassan, Mahmoud A.E. (Animal Production Research Institute, Dokki, Giza, Egypt ) , Mohey-Elsaeed, Omnia (Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt)
    International journal of veterinary science and medicine v.6 suppl. ,pp. S49 - S56 , 2018 , 2314-4599 ,

    초록

    Abstract Semen cryopreservation is a well-established procedure used in veterinary assisted reproduction technology applications. We investigated damaging effects of cryopreservation on the structural and ultrastructural characteristics of bull sperm induced at different temperatures and steps during standard cryopreservation procedure using transmission (TEM) and scanning electron microscopy. We also examined the effect of cryopreservation on sperm DNA and chromatin integrity. Five healthy, fertile Friesian bulls were used, and the ejaculates were obtained using an artificial vagina method. The semen samples were pooled and diluted in a tris-yolk fructose (TYF) for a final concentration of 80 × 10 6 spermatozoa/ml. The semen samples were packed in straws (0.25 ml), and stored in liquid nitrogen (−196°C). Samples were evaluated before dilution, just after dilution (at 37°C), at 2 h and 4 h during equilibration, and after thawing (37°C for 30 s in water bath). In association with step-wise decline in motility and viability, our results showed that the plasma membrane surrounding the sperm head was the most vulnerable structure to cryo-damage with various degrees of swelling, undulation, or loss affecting about 50% of the total sperm population after equilibration and freezing. Typical acrosome reaction was limited to 10% of the spermatozoa after freezing. We also observed increased number of mitochondria with distorted cristae (15%). Chromatin damage was significantly increased by cryopreservation as evident by TEM (9%). This was mainly due to DNA breaks as confirmed by Sperm Chromatin Structure Assay (SCSA) (8.4%) whereas the chromatin structure was less affected as evaluated microscopically by toluidine blue staining. We concluded that, using standard cryopreservation protocol, the most pronounced damage induced by cryopreservation is observed in the plasma membrane. Further improvement of cryopreservation protocols should thus be targeted at reducing plasma membrane damage. Acrosomal, mitochondrial and chromatin damage are also evident but appear to be within acceptable limits as discussed.

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  3. [해외논문]   Sex differences in single IVF-derived bovine embryo cultured in chemically defined medium  

    Saadeldin, Islam M. (Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, 11451 Riyadh, Saudi Arabia ) , Jang, Goo (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, 08826, South Korea)
    International journal of veterinary science and medicine v.6 suppl. ,pp. S78 - S80 , 2018 , 2314-4599 ,

    초록

    Abstract Single embryo culture is essential for culturing embryos derived from few oocytes obtained from elite cows through ultrasonography guidance. Bovine in vitro fertilization (IVF) and individual embryo culture is a challenge as it generally leads to impaired embryo development. In this study, we explored the embryonic development and the sex ratio of IVF-derived bovine embryo cultured individually in chemically defined two-step culture medium. Total 63 cumulus-oocyte complexes were collected, in vitro matured, in vitro fertilized and the resultant fertilized oocytes were randomly cultured individually (4 trials, 15–16 oocytes each) in microdrops of 5 μL of a chemically defined two-step culture medium. Blastocysts were counted in every trial (n = 32, 50.79%) and all of them were used for both genomic DNA and total RNA extraction, cDNA synthesis and PCR using specific primers for GAPDH , GDP6 , XIST and SRY genes. Results showed significant difference in expression of XIST (positive expression in 11 blastocysts) and SRY (positive expression in 21 blastocysts) mRNAs, P in vitro produced embryos and provides an efficient medium for single bovine embryos in vitro production.

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  4. [해외논문]   Effect of oviduct and follicular fluids on ram sperm capacitation and acrosome reaction in vitro  

    El-Shahat, K.H. (Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Egypt ) , Taysser, M.I. (Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Egypt ) , Badr, M.R. (Department of Animal Reproduction and Artificial Insemination, Reproduction Research Institute, Agriculture Research Center, Egypt ) , Zaki, K.A. (El Shieh Zaied Veterinary Clinic, General Egyptian Organization of Vet. Service, Egypt)
    International journal of veterinary science and medicine v.6 suppl. ,pp. S57 - S62 , 2018 , 2314-4599 ,

    초록

    Abstract The present study was designed to study the influence of different concentrations of oviduct fluid (OF) and follicular fluid (FF) on ram sperm capacitation and acrosome reaction in vitro. Forty semen samples were collected from three Barki rams throughout the period of study (10 weeks). Fresh semen was evaluated, layered under S-TALP (sperm Tyrode’s albumin lactate pyruvate) medium and subjected to swim up techniques. Split fractions of semen were incubated in media enriched with different levels of OF (10, 20, 40, 50 and 75 μL/mL) or FF (10, 20, 40, 50, 100, 150 and 200 μL/mL). Best concentrations were compared and used to evaluate the ram sperm functions including progressive motility, hyperactivity and acrosome reaction. The present findings showed a significant increase in individual motility percentage (IM %) when ram spermatozoa were treated with 10 and 50 μL of oviduct fluids as compared to the other treatments after 1 h of incubation. Addition of 50 μL/mL of OF or FF had beneficial effect on sperm hyperactivity after 2 and 1 h incubation respectively. Furthermore, addition of 50 μL/mL oviduct fluid to ram sperm maintained significantly ( P

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  5. [해외논문]   The effect of cadmium on the bovine in vitro oocyte maturation and early embryo development  

    Akar, Yaş (Deparment of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, University of Erciyes, Talas, Kayseri, Turkey ) , ar (Deparment of Environmental Sciences, COMSATS Institute of Information Technology, Vehari, Pakistan ) , Ahmad, Naveed (Department of Pathobiology and Population Sciences, The Royal Veterinary College, University of London, London, UK) , Khalıd, Muhammad
    International journal of veterinary science and medicine v.6 suppl. ,pp. S73 - S77 , 2018 , 2314-4599 ,

    초록

    Abstract Common pollutants such as heavy metals and cadmium is among those with high environmental concerns. In vivo studies had shown that cadmium (Cd) causes oocyte degeneration and embryo mortality, and lowers pregnancy rates in mammals. However, there is limited information available about direct effects of Cd on oocyte maturation and/or embryo development. This study was aimed to investigate if Cd has any effect on the oocyte maturation and/or embryo development in vitro . Bovine COCs were collected from the slaughter house and cultured for 24 h in serum-free media only (Controls) or supplemented with 0.2, 2.0 and 20.0 μM CdCl 2 . At 24 h cumulus cell expansion was assessed in all COCs. COCs were either denuded and stained for determination of nuclear maturation or fertilized for assessment of subsequent embryo development. Cd at the lowest concentration (0.2 μM) did not affect any of the parameters studied. However, at higher concentrations (2.0 and 20.0 μM) it significantly ( P P P P 2 compared to the controls (0.0 μM). In conclusion, these results suggest that Cd had direct detrimental effects on the bovine oocyte maturation and its developmental competence.

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  6. [해외논문]   Polyunsaturated fatty acids influence offspring sex ratio in cows  

    Marei, Waleed F.A. (Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt ) , Khalil, Wael A. (Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt ) , Pushpakumara, Anil P.G. (Department of Farm Animal Production and Health, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka ) , El-Harairy, Mostafa A. (Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt ) , Abo El-Atta, Ahmed M.A. (Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt ) , Wathes, D. Claire (Department of Pathobiology and Population Sciences, Royal Veterinary College, Hatfield AL9 7TA, UK ) , Fouladi-Nashta, Ali (Department of Comparative Biomedical Sciences, Royal Veterinary College, Hatfield AL9 7TA, UK)
    International journal of veterinary science and medicine v.6 suppl. ,pp. S36 - S40 , 2018 , 2314-4599 ,

    초록

    Abstract Dietary polyunsaturated fatty acids (PUFAs) can influence fertility in farm animals. Some evidence in mice and sheep have suggested that PUFAs may influence offspring sex ratio, which may have significant value for cattle production. To test this hypothesis, three groups of Holstein cows were supplemented with either 0%, 3% or 5% protected fat (PF) in the form of calcium salt of fatty acids (rich in omega-6) from 14–21 days pre-partum until conception. Proven-fertile frozen semen from the same ejaculate was used for insemination. Calf sex recorded at birth was 8/19 (42.1%) male offspring in the control group, increasing to 14/20 (70%, P > 0.05) and 17/20 (85%, P in vitro maturation with either omega-3 alpha-linolenic acid (ALA), omega-6 linoleic acid (LA) or trans-10, cis-12 conjugated linoleic acid (CLA). Sex ratio of the produced transferable embryos was determined using PCR of SRY gene. Similar to the in vivo results, sex ratio was skewed to the male side in the embryos derived from LA- and CLA-treated oocytes (79% and 71%) compared to control and ALA-treated oocytes (44% and 54%, respectively). These results indicate that both dietary and in vitro supplementation of omega-6 PUFAs can skew the sex ratio towards the male side in cattle. Further experiments are required to confirm this effect on a larger scale and to study the mechanisms of action that might be involved.

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  7. [해외논문]   Brucellosis: Evolution and expected comeback  

    El-Sayed, Amr (Corresponding author at: Faculty of Veterinary Medicine, Giza Square, 11451 Giza, Egypt.) , Awad, Walid
    International journal of veterinary science and medicine v.6 suppl. ,pp. S31 - S35 , 2018 , 2314-4599 ,

    초록

    Abstract Brucellosis is a serious infectious disease which causes great direct and indirect economic loses for animal holders worldwide such as the reduction of milk and meat production through abortions/culling of positive reactors, the expense of disease control/eradication and farmers compensation. Although the disease was eradicated from most of the industrial countries, it remains one of the most common zoonotic diseases in developing countries being responsible for more than 500,000 new cases yearly. Brucella is considered to be a bioterrorism organism due to its low infectious doses (10–100 bacteria), capability of persistence in the environment, rapid transmission via different routes including aerosols, and finally due to its difficult treatment by antibiotics.There are many reasons to believe that a new comeback of brucellosis may occur in near future. This expectation is supported by the recent discovery of new atypical Brucella species with new genetic properties and the recent reports of (man to man) disease transmission as will be discussed later. The development of new concepts and measurements for disease control is urgently required. In the present review, the evolution of Brucella and the different factors favoring its comeback are discussed.

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  8. [해외논문]   Advances in in vitro production of sheep embryos  

    Zhu, Jie (The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070, China ) , Moawad, Adel R. (Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, PO BOX 12211, Giza, Egypt ) , Wang, Chun-Yu (The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070, China ) , Li, Hui-Feng (The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070, China ) , Ren, Jing-Yu (The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070, China ) , Dai, Yan-Feng (The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010070, China)
    International journal of veterinary science and medicine v.6 suppl. ,pp. S15 - S26 , 2018 , 2314-4599 ,

    초록

    Abstract Sheep is an important livestock in the world providing meat, milk and wool for human beings. With increasing human population, the worldwide needs of production of sheep have elevated. To meet the needs, the assistant reproductive technology including ovine in vitro embryo production (ovine IVP) is urgently required to enhance the effective production of sheep in the world. To learn the status of ovine IVP, we collected some publications related to ovine IVP through PubMed and analyzed the progress in ovine IVP made in the last five years (2012–2017). We made comparisons of these data and found that the recent advances in ovine IVP has been made slowly comparable to that of ovine IVP two decades ago. Therefore, we suggested two strategies or approaches to tackle the main problems in ovine IVP and expect that the efficiency of ovine IVP could be improved significantly when the approaches would be implemented.

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  9. [해외논문]   IFC-Editorial Board  


    International journal of veterinary science and medicine v.6 suppl. ,pp. IFC - IFC , 2018 , 2314-4599 ,

    초록

    Abstract Sheep is an important livestock in the world providing meat, milk and wool for human beings. With increasing human population, the worldwide needs of production of sheep have elevated. To meet the needs, the assistant reproductive technology including ovine in vitro embryo production (ovine IVP) is urgently required to enhance the effective production of sheep in the world. To learn the status of ovine IVP, we collected some publications related to ovine IVP through PubMed and analyzed the progress in ovine IVP made in the last five years (2012–2017). We made comparisons of these data and found that the recent advances in ovine IVP has been made slowly comparable to that of ovine IVP two decades ago. Therefore, we suggested two strategies or approaches to tackle the main problems in ovine IVP and expect that the efficiency of ovine IVP could be improved significantly when the approaches would be implemented.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  10. [해외논문]   Cytokines, growth factors and macromolecules as mediators of implantation in mammalian species  

    Raheem, Kabir A.
    International journal of veterinary science and medicine v.6 suppl. ,pp. S6 - S14 , 2018 , 2314-4599 ,

    초록

    Abstract Implantation is one of the most critical steps in mammalian reproduction and implantation failure constitutes a major cause of infertility in both animals and humans. The mechanism of implantation is exclusively under the control of ovarian steroids progesterone and oestrogen whose actions are mediated in a complex phenomenon that involves a number of cytokines and growth factors. According to a plethora of literature on implantation in mammalian species, prominent of these cytokines and growth factor playing crucial roles in implantation include integrin, osteopontin, integrin, insulin-like growth factor and leukaemia inhibitory factor. Others are cluster domain 44, hyaluronan system and many non-adhesive molecules such as glycoprotein mucin 1. In this review, the specific roles played by these molecules are expatiated. Generally, they function as adhesive molecules that facilitate attachment of ligands/proteins on the trophectoderm to their respective receptors on endometrial luminal epithelia or vice versa. Sometimes, they also function as signalling molecules that enhance communication between implanting blastocyst and receptive endometrium. This is of particular importance in embryo culture and embryo transfer where in vitro derived blastocyst unlike the in vivo condition, is not exposed to these substances and hence, their absence may be partly responsible for the low implantation rate observed in the surrogate. Appreciation of the roles played by these cytokines, growth factors and molecules as revealed in this review will spur further research on these topics, facilitate their inclusion in embryo culture media (if positively required) and are considered as vital aspect while developing strategies to improve fertility.

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