본문 바로가기
HOME> 저널/프로시딩 > 저널/프로시딩 검색상세

저널/프로시딩 상세정보

권호별목차 / 소장처보기

H : 소장처정보

T : 목차정보

Experimental and molecular pathology 19건

  1. [해외논문]   Somatic mutation analysis in melanoma using targeted next generation sequencing   SCIE SCOPUS

    Miraflor, Allen P. (Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, United States ) , de Abreu, Francine B. (Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, United States ) , Peterson, Jason D. (Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, United States ) , Turner, Scott A. (Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, United States ) , Amos, Christopher I. (Department of Biomedical Data Science, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, United States ) , Tsongalis, Gregory J. (Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, United States ) , Yan, Shaofeng (Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, United States)
    Experimental and molecular pathology v.103 no.2 ,pp. 172 - 177 , 2017 , 0014-4800 ,

    초록

    Abstract Advanced stage malignant melanoma often responds poorly to therapy with low survival rates. New therapeutic approaches are based upon a growing understanding of the underlying molecular abnormalities. We demonstrate the feasibility of a next generation sequencing (NGS) assay, which targets hotspots in 50 cancer genes, to assess genotypes that may influence therapeutic selection and response. DNA was extracted from formalin fixed paraffin embedded (FFPE) melanoma specimens to create multiplexed libraries which were sequenced. Of the 121 cases, BRAF mutations were present in 48 cases (40%) and NRAS mutations in 24 cases (20%). We identified other gene variants in 20 BRAF -mutated cases. Additional gene variants were also identified in the 57 BRAF wild-type cases. Four patients harbored different gene mutations at metastatic sites as compared to their primary lesions or metastasis from different sites. Concurrent gene variants may provide additional targets for future therapies and may suggest alternative mechanisms of secondary resistance.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  2. [해외논문]   Case report: An HIV+ patient presenting with pancytopenia, hepatic failure, and coagulopathy; a rare small cell liver carcinoma with diffuse splenic and bone marrow metastasis diagnosed at autopsy   SCIE SCOPUS

    Byers, Joshua T. (Department of Pathology, Harbor-UCLA Medical Center, 1000 W. Carson St, Torrance, CA, United States ) , Mendoza, Alejandro (Department of Pathology, Harbor-UCLA Medical Center, 1000 W. Carson St, Torrance, CA, United States ) , Wu, Daniel (Department of Hematology and Oncology, Harbor-UCLA Medical Center, 1000 W. Carson St, Torrance, CA, United States ) , Kahlon, Jason S. (Department of Internal Medicine, Harbor-UCLA Medical Center, 1000 W. Carson St, Torrance, CA, United States ) , Qing, Xin (Department of Pathology, Harbor-UCLA Medical Center, 1000 W. Carson St, Torrance, CA, United States ) , French, Samuel W. (Department of Pathology, Harbor-UCLA Medical Center, 1000 W. Carson St, Torrance, CA, United States)
    Experimental and molecular pathology v.103 no.2 ,pp. 178 - 180 , 2017 , 0014-4800 ,

    초록

    Abstract A 34-year old male with a giant condyloma acuminatum of the anus secondary to HIV infection presented to the emergency department with a persistent nose bleed lasting 2–3days, acute anemia, thrombocytopenia, and coagulopathy. The patient also had significant hepatosplenomegaly and elevated liver enzymes which were a new finding since the patient's last hospitalization 1–2month prior to the current admission. A bone marrow biopsy showed diffuse infiltration by carcinoma with neuroendocrine features. The patient quickly developed multi-organ injury, decompensated, and died. An autopsy was obtained which established the diagnosis of small cell carcinoma of the liver.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  3. [해외논문]   T cell-mediated antitumor immune response eliminates skin tumors induced by mouse papillomavirus, MmuPV1   SCIE SCOPUS

    Joh, Joongho (James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA ) , Chilton, Paula M. (Christine M. Kleinert Institute for Hand & Microsurgery, 225 Abraham Flexner Way, Suite 850, Louisville, KY, USA ) , Wilcher, Sarah A. (Research Resources Center, 530 South Jackson Street, Louisville, KY, USA ) , Zahin, Maryam (James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA ) , Park, Jino (James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA ) , Proctor, Mary L. (Research Resources Center, 530 South Jackson Street, Louisville, KY, USA ) , Ghim, Shin-je (James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA ) , Jenson, Alfred B. (James Graham Brown Cancer Center, University of Louisville, Louisville, KY, USA)
    Experimental and molecular pathology v.103 no.2 ,pp. 181 - 190 , 2017 , 0014-4800 ,

    초록

    Abstract Previous studies of naturally occurring mouse papillomavirus (PV) MmuPV1-induced tumors in B6.Cg- Foxn1 nu/nu mice suggest that T cell deficiency is necessary and sufficient for the development of such tumors. To confirm this, MmuPV1-induced tumors were transplanted from T cell-deficient mice into immunocompetent congenic mice. Consequently, the tumors regressed and eventually disappeared. The elimination of MmuPV1-infected skin/tumors in immunocompetent mice was consistent with the induction of antitumor T cell immunity. This was confirmed by adoptive cell experiments using hyperimmune splenocytes collected from graft-recipient mice. In the present study, such splenocytes were injected into T cell-deficient mice infected with MmuPV1, and they eliminated both early-stage and fully formed tumors. We clearly show that anti-tumor T cell immunity activated during tumor regression in immunocompetent mice effectively eliminates tumors developing in T cell-deficient congenic mice. The results corroborate the notion that PV-induced tumors are strongly linked to the immune status of the host, and that PV antigens are major anti-tumor antigens. Successful anti-PV T cell responses should, therefore, lead to effective anti-tumor immune therapy in human PV-infected patients.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  4. [해외논문]   The role of Tec kinase signaling pathways in the development of Mallory Denk Bodies in balloon cells in alcoholic hepatitis   SCIE SCOPUS

    Afifiyan, N. (Corresponding author.) , Tillman, B. , French, B.A. , Sweeny, O. , Masouminia, M. , Samadzadeh, S. , French, S.W.
    Experimental and molecular pathology v.103 no.2 ,pp. 191 - 199 , 2017 , 0014-4800 ,

    초록

    Abstract Several research strategies have been used to study the pathogenesis of alcoholic hepatitis (AH). These strategies have shown that various signaling pathways are the target of alcohol in liver cells. However, few have provided specific mechanisms associated with Mallory-Denk Bodies (MDBs) formed in Balloon cells in AH. The formation of MDBs in these hepatocytes is an indication that the mechanisms of protein quality control have failed. The MDB is the result of aggregation and accumulation of proteins in the cytoplasm of balloon degenerated liver cells. To understand the mechanisms that failed to degrade and remove proteins in the hepatocyte from patients suffering from alcoholic hepatitis, we investigated the pathways that showed significant up regulation in the AH liver biopsies compared to normal control livers (Liu et al., 2015). Analysis of genomic profiles of AH liver biopsies and control livers by RNA-seq revealed different pathways that were up regulated significantly. In this study, the focus was on Tec kinase signaling pathways and the genes that significantly interrupt this pathway. Quantitative PCR and immunofluorescence staining results, indicated that several genes and proteins are significantly over expressed in the livers of AH patients that affect the Tec kinase signaling to PI3K which leads to activation of Akt and its downstream effectors.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  5. [해외논문]   Hepatocellular carcinoma with neuroendocrine differentiation: a case report   SCIE SCOPUS

    Lu, Jiajie G. (Harbor UCLA Medical Center, Department of Pathology, 1000 West Carson Street, Torrance, CA 90502, United States ) , Farukhi, M. Aabid (Department of Radiology, 1000 West Carson Street, Torrance, CA 90502, United States ) , Mayeda, Donna (Department of Internal Medicine, 1000 West Carson Street, Torrance, CA 90502, United States ) , French, Samuel W. (Harbor UCLA Medical Center, Department of Pathology, 1000 West Carson Street, Torrance, CA 90502, United States)
    Experimental and molecular pathology v.103 no.2 ,pp. 200 - 203 , 2017 , 0014-4800 ,

    초록

    Abstract Hepatocellular carcinoma with neuroendocrine differentiation, where tumor cells stain for both hepatocellular and neuroendocrine markers, is extremely rare. We report a case of a 65-year-old man who presented with a 14-cm rapidly growing mass in the right lobe of his liver with local extension into the gallbladder and portal vein. Serum AFP was 4625ng/mL. Liver biopsy showed a poorly differentiated neoplasm with cells showing nuclear pleomorphism, high nuclear/cytoplasmic ratio, and numerous mitoses. The tumor cells stain for AFP, glutamine synthase, arginase, and glypican-3. The same tumor regions also stain positively for synaptophysin, chromogranin, and CD56. Given this histological pattern, this tumor was ultimately diagnosed as hepatocellular carcinoma with neuroendocrine differentiation.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  6. [해외논문]   The adaptor protein alpha-syntrophin is reduced in human non-alcoholic steatohepatitis but is unchanged in hepatocellular carcinoma   SCIE SCOPUS

    Rein-Fischboeck, Lisa (Department of Internal Medicine I, Regensburg University Hospital, Regensburg, Germany ) , Pohl, Rebekka (Department of Internal Medicine I, Regensburg University Hospital, Regensburg, Germany ) , Haberl, Elisabeth M. (Department of Internal Medicine I, Regensburg University Hospital, Regensburg, Germany ) , Weiss, Thomas S. (Children's University Hospital (KUNO), Regensburg University Hospital, Regensburg, Germany ) , Buechler, Christa (Department of Internal Medicine I, Regensburg University Hospital, Regensburg, Germany)
    Experimental and molecular pathology v.103 no.2 ,pp. 204 - 209 , 2017 , 0014-4800 ,

    초록

    Abstract The adaptor protein alpha-syntrophin (SNTA) is differentially expressed in varying types of cancer and affects triglyceride levels, inflammatory response and cell proliferation. However, little is known about the expression of SNTA in liver diseases. Non-alcoholic steatohepatitis (NASH) is characterized by hepatic steatosis, inflammation and eventually fibrosis, and may progress to hepatocellular carcinoma (HCC). Here, SNTA mRNA was analyzed in liver tissues from 71 non-alcoholic fatty liver disease patients and 32 controls to assess associations with disease characteristics. SNTA mRNA expression was reduced in NASH liver and negatively correlated with steatosis, inflammation, fibrosis and NASH scores. In the NASH patients, those with type 2 diabetes had a higher fibrosis score, reduced inflammation and increased hepatic SNTA mRNA levels demonstrating a strong association of SNTA mRNA levels with inflammation. Recently, we have shown diminished expression of the high-density lipoprotein scavenger receptor BI (SR-BI) in the liver of syntrophin-deficient mice. Indeed, hepatic SNTA and SR-BI mRNA were positively correlated. SNTA protein was further determined in tumor and non-tumorous tissues of 21 HCC patients. Protein expression was unchanged in the tumor and not related to staging and grading. Present study identified associations of hepatic SNTA mRNA levels with SR-BI and features of NASH assuming a function of this protein in chronic liver disease and cholesterol metabolism. Highlights The mRNA level of alpha-syntrophin (SNTA) is reduced in non-alcoholic steatohepatitis. Hepatic SNTA mRNA is negatively correlated with features of NASH. Hepatic SNTA mRNA and SR-BI mRNA are positively correlated. SNTA protein is not changed in hepatocellular carcinoma.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  7. [해외논문]   Sphingosine 1-phosphate alleviates Coxsackievirus B3-induced myocarditis by increasing invariant natural killer T cells   SCIE SCOPUS

    Wang, Xinggang (Corresponding author at: Room 217, Building 19, No. 1609, Xietu Road, Xuhui District, Shanghai 200032, China.) , Yu, Yong , Li, Minghui , Yu, Ying , Liu, Guijian , Xie, Yeqing , Liu, Yuxi , Yang, Xiangdong , Zou, Yunzeng , Ge, Junbo , Chen, Ruizhen
    Experimental and molecular pathology v.103 no.2 ,pp. 210 - 217 , 2017 , 0014-4800 ,

    초록

    Abstract Sphingosine 1-phosphate (S1P), via binding to its specific receptors of S1PR1, participates in the regulation of both innate and adaptive immunity. Recent reports have identified S1P as a messenger mediating inflammation. However, roles of S1P in Coxsackievirus B3 (CVB3)-induced myocarditis were largely unknown. Here, we investigated the effect of S1P treatment on CVB3-induced myocarditis in vivo. We found that CVB3 infection downregulated S1PR1 expression in spleen and decreased the proportion of invariant natural killer T cells (iNKT) in CD3 positive T cells both in spleen and in blood from left ventricle, which accompanied by severe inflammation lesions and more virus capsid protein (VP1) expression in heart tissue. In comparison, S1P supply upregulated iNKT in the spleen and in blood from left ventricle, which represented the strengthening of anti-inflammatory effects. Indeed, inflammation infiltration, VP1 expression and apoptosis in the myocardium was all downregulated. These results demonstrated that S1P supplement could alleviate CVB3-induced myocarditis.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  8. [해외논문]   Cytoplasmic expression of CD133 stemness marker is associated with tumor aggressiveness in clear cell renal cell carcinoma   SCIE SCOPUS

    Saeednejad Zanjani, Leili (Oncopathology Research Centre, Iran University of Medical Sciences (IUMS), Tehran, Iran ) , Madjd, Zahra (Oncopathology Research Centre, Iran University of Medical Sciences (IUMS), Tehran, Iran ) , Abolhasani, Maryam (Oncopathology Research Centre, Iran University of Medical Sciences (IUMS), Tehran, Iran ) , Andersson, Yvonne (Department of Tumor Biology, Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway ) , Rasti, Arezoo (Oncopathology Research Centre, Iran University of Medical Sciences (IUMS), Tehran, Iran ) , Shariftabrizi, Ahmad (Department of Nuclear Medicine and Molecular Imaging, State University of New York at Buffalo, Buffalo, NY 14223, USA ) , Asgari, Mojgan (Oncopathology Research Centre, Iran University of Medical Sciences (IUMS), Tehran, Iran)
    Experimental and molecular pathology v.103 no.2 ,pp. 218 - 228 , 2017 , 0014-4800 ,

    초록

    Abstract Prominin-1 (CD133) is one of the most commonly used markers for cancer stem cells (CSCs), which are characterized by their ability for self-renewal and tumorigenicity. However, the clinical and prognostic significance of CSCs in renal cell carcinoma (RCC) remains unclear. The aim of this study was to investigate the expression patterns and prognostic significance of the cancer stem cell marker CD133 in different histological subtypes of RCC. CD133 expression was evaluated using immunohistochemistry in 193 well-defined renal tumor samples on tissue microarrays, including 136 (70.5%) clear cell renal cell carcinomas (CCRCCs), 26 (13.5%) papillary RCCs, and 31 (16.1%) chromophobe RCCs. The association between CD133 expression and clinicopathological features as well as the survival outcomes was determined. There was a statistically significant difference between CD133 expression among the different RCC subtypes. In CCRCC, higher cytoplasmic expression of CD133 was significantly associated with increase in grade, stage, microvascular invasion (MVI) and lymph node invasion (LNI), while no association was found with the membranous expression. Moreover, on multivariate analysis, TNM stage and nuclear grade were independent prognostic factors for overall survival (OS) in cytoplasmic expression. We showed that higher cytoplasmic CD133 expression was associated with more aggressive tumor behavior and more advanced disease in CCRCC but not in the other examined subtypes. Our results demonstrated that higher cytoplasmic CD133 expression is clinically significant in CCRCC and is associated with increased tumor aggressiveness and is useful for predicting cancer progression. Highlights Aimed to investigate the membranous and cytoplasmic CD133 expression as a cancer stem cell marker and its correlation with tumor clinicopathological parameters of RCC samples, including clear cell, chromophobe, and papillary RCC. CD133 shift from membranous to cytoplasmic localization, is associated with a more invasive phenotype. High cytoplasmic CD133 expression was associated with increased tumor invasiveness and more advanced disease. Cytoplasmic CD133 expression is useful for predicting cancer progression in clear cell RCC patients but not in the other subtypes.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  9. [해외논문]   MicroRNA-21 and long non-coding RNA MALAT1 are overexpressed markers in medullary thyroid carcinoma   SCIE SCOPUS

    Chu, Ying-Hsia (Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, United States ) , Hardin, Heather (Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, United States ) , Schneider, David F. (Department of Surgery, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, United States ) , Chen, Herbert (Department of Surgery, University of Alabama at Birmingham, Birmingham, AL 35294, United States ) , Lloyd, Ricardo V. (Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, United States)
    Experimental and molecular pathology v.103 no.2 ,pp. 229 - 236 , 2017 , 0014-4800 ,

    초록

    Abstract Background Non-coding RNAs, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), are well-recognized post-transcriptional regulators of gene expression. This study examines the expression of microRNA-21 (miR-21) and lncRNA MALAT1 in medullary thyroid carcinomas (MTCs) and their effects on tumor behavior. Methods Tissue microarrays (TMAs) were constructed using normal thyroid (n=39), primary tumors (N=39) and metastatic MTCs (N=18) from a total of 42 MTC cases diagnosed between 1987 and 2016. In situ hybridization with probes for miR-21 and MALAT1 was performed. PCR quantification of expression was performed in a subset of normal thyroid (N=10) and primary MTCs (N=32). An MTC-derived cell line (MZ-CRC-1) was transfected with small interfering RNAs (siRNAs) targeting miR-21 and MALAT1 to determine the effects on cell proliferation and invasion. Results In situ hybridization (ISH) showed strong (2+ to 3+) expression of miR-21 in 17 (44%) primary MTCs and strong MALAT1 expression in 37 (95%) primary MTCs. Real-time PCR expression of miR-21 ( P P = 0.038) in primary MTCs were significantly higher than in normal thyroid, supporting the ISH findings. Experiments with siRNAs showed inhibition of miR-21 and MALAT1 expression in the MTC-derived cell line, leading to significant decreases in cell proliferation ( P P Conclusion There is increased expression of miR-21 and MALAT1 in MTCs. This study also showed an in vitro pro-oncogenic effect of MALAT1 and miR-21 in MTCs. The results suggest that overexpression of miR-21 and MALAT1 may regulate MTC progression.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지

논문관련 이미지