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Archives of pharmacal research : a publication of ... 23건

  1. [국내논문]   Agastache rugosa Leaf Extract Inhibits the iNOS Expression in ROS 17/2.8 Cells Activated with TNF-$\alpha$ and IL-$\beta$   피인용횟수: 2

    Oh Hwa Min (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University ) , Kang Young Jin (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University ) , Kim Sun Hee (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University ) , Lee Young Soo (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University ) , Park Min Kyu (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University ) , Heo Ja Myung (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University ) , Sun Jin Ji (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University ) , Kim Hyo Jung (Department of Pharmacology, College of Medicine, and Institute of Health Sciences, Gyeongsang National University ) , Kang Eun Sil (Department of Pharmacology, College of Medicine, and Institute of Health) , Kim Hye Jung , Sea Han Geuk , Lee Jae Heun , YunChoi Hye Sook
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 305 - 310 , 2005 , 0253-6269 ,

    초록

    It has been suggested that nitric oxide (NO) derived from inducible nitric oxide synthase (iNOS) may act as a mediator of cytokine-induced effects on bone turn-over. NO is also recognized as an important factor in bone remodeling, i.e., participating in osteoblast apoptosis in an arthritic joint. The components of Agastache rugosa are known to have many pharmacological activities. In the present study, we investigated the effects of Agastache rugosa leaf extract (ELAR) on NO production and the iNOS expression in ROS 17/2.8 cells activated by a mixture of inflammatory cytokines including TNF- $alpha$ and IL-1 $\beta$ . A preincubation with ELAR significantly and concentration-dependently reduced the expression of iNOS protein in ROS 17/2.8 cells activated with the cytokine mixture. Consequently, the NO production was also significantly reduced by ELAR with an IC $_{50}$ of 0.75 mg/mL. The inhibitory mechanism of iNOS induction by ELAR prevented the activation and translocation of NF- $\kappa$ B (p65) to the nucleus from the cytosol fraction. Furthermore, ELAR concentration-dependently reduced the cellular toxicity induced by sodium nitroprusside, an NO-donor. These results suggest that ELAR may be beneficial in NO-mediated inflammatory conditions such as osteoporosis.

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  2. [국내논문]   Protection of LLC-PK1 Cells Against Hydrogen Peroxide­Induced Cell Death by Modulation of Ceramide Level  

    Yoo Jae Myung (College of Pharmacy, Chungbuk National University ) , Lee Youn Sun (College of Pharmacy, Chungbuk National University ) , Choi Heon Kyo (College of Pharmacy, Chungbuk National University ) , Lee Yong Moon (College of Pharmacy, Chungbuk National University ) , Hong Jin Tae (College of Pharmacy, Chungbuk National University ) , Yun Yeo Pyo (College of Pharmacy, Chungbuk National University ) , Oh Seik Wan (College of Medicine, Ewha Womans University ) , Yoo Hwan Soo (College of Pharmacy, Chungbuk National University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 311 - 318 , 2005 , 0253-6269 ,

    초록

    Oxidative stress has been reported to elevate ceramide level during cell death. The purpose of the present study was to modulate cell death in relation to cellular glutathione (GSH) level and GST (glutathione S-transferase) expression by regulating the sphingolipid metabolism. LLC­PK1 cells were treated with H $_2$ O $_2$ in the absence of serum to induce cell death. Subsequent to exposure to H $_2$ O $_2$ , LLC-PK1 cells were treated with desipramine, sphingomyelinase inhibitor, and N-acetylcysteine (NAC), GSH substrate. Based on comparative visual observation with H202-treated control cells, it was observed that 0.5 $\mu$ M of desipramine and 25 $\mu$ M of NAC exhibited about 90 and $95\%$ of cytoprotection, respectively, against H $_2$ O $_2$ -induced cell death. Desipramine and NAC lowered the release of LDH activity by 36 and $3\%$ respectively, when compared to $71\%$ in H $_2$ O $_2$ -exposed cells. Cellular glutathione level in 500 $\mu$ M H202-treated cells was reduced to 890 pmol as compared to control level of 1198 pmol per mg protein. GST P1-1 expression was decreased in H $_2$ O $_2$ -treated cells compared to healthy normal cells. In conclusion, it has been inferred that H $_2$ O $_2$ -induced cell death is closely related to cellular GSH level and GST P1-1 expression in LLC-PK1 cells and occurs via ceramide elevation by sphingomyelinase activation.

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  3. [국내논문]   The Difference Between Nicardipine and Its Enantiomers on Inhibiting Vasoconstriction of Isolated Rabbit Thoracic Artery  

    Wang Sicen (College of Pharmacy, Xi'an Jiaotong University ) , He Lang chong (College of Pharmacy, Xi'an Jiaotong University ) , Yun Sofeng (College of Pharmacy, Xi'an Jiaotong University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 319 - 324 , 2005 , 0253-6269 ,

    초록

    The present study was designed to study the difference effects between nicardipine and its two enantiomers on thoracic artery of rabbit. A high-performance liquid chromatographic method was used to prepare the two enantiomers of nicardipine. The thoracic artery of rabbit was removed. The vessels were cut into 3 mm in width and 15 mm in length spiral strips and immersed into tissue baths. The concentration-response curves of nicardipine and its enantiomers were obtained by cumulative administration of the vasoconstrictors. Nicardipine and the enantiomers could shift the dose-response curves of NE, KCI or CaCl $_2$ to right in a nonparallel manner and decrease the maximum effective in a concentration-depended manner, respectively. The pD $_2$ ' value of R-(-)-nicardipine showed significantly effective than that of nicardipine and S-(+)-nicardipine (P $_2$ ' value of nicardipine and S-(+)-nicardipine (P>0.05). The results demonstrate that the stereoselective interaction between R-(-)-nicardipine and L-calcium channel receptor is more stronger than that of S-(+)-nicardipine.

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  4. [국내논문]   Hepatoprotective effect of lactic acid bacteria, inhibitors of β-glucuronidase production against intestinal microflora  

    Han, Song-Yi , Huh, Chul-Sung , Ahn, Young-Tae , Lim, Kwang-Sei , Baek, Young-Jin , Kim, Dong-Hyun
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 325 - 329 , 2005 , 0253-6269 ,

    초록

    The present study was designed to study the difference effects between nicardipine and its two enantiomers on thoracic artery of rabbit. A high-performance liquid chromatographic method was used to prepare the two enantiomers of nicardipine. The thoracic artery of rabbit was removed. The vessels were cut into 3 mm in width and 15 mm in length spiral strips and immersed into tissue baths. The concentration-response curves of nicardipine and its enantiomers were obtained by cumulative administration of the vasoconstrictors. Nicardipine and the enantiomers could shift the dose-response curves of NE, KCI or CaCl $_2$ to right in a nonparallel manner and decrease the maximum effective in a concentration-depended manner, respectively. The pD $_2$ ' value of R-(-)-nicardipine showed significantly effective than that of nicardipine and S-(+)-nicardipine (P $_2$ ' value of nicardipine and S-(+)-nicardipine (P>0.05). The results demonstrate that the stereoselective interaction between R-(-)-nicardipine and L-calcium channel receptor is more stronger than that of S-(+)-nicardipine.

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  5. [국내논문]   Hepatoprotective Effect of Lactic Acid Bacteria, Inhibitors of $\beta$-Glucuronidase Production Against Intestinal Microflora   피인용횟수: 2

    Han Song Yi (College of Pharmacy, Kyung Hee University ) , Huh Chul Sung (R & D center, Korea Yakult Co., Ltd. ) , Ahn Young Tae (R & D center, Korea Yakult Co., Ltd. ) , Lim Kwang Sei (R & D center, Korea Yakult Co., Ltd. ) , Baek Young Jin (R & D center, Korea Yakult Co., Ltd. ) , Kim Dong Hyun (College of Pharmacy, Kyung Hee University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 325 - 329 , 2005 , 0253-6269 ,

    초록

    The hepatoprotective activity of lactic acid bacteria (Lactobacillus brevis HY7401, Lactobacillus acidophilus CSG and Bifidobacterium longum HY8001), which inhibited $\beta$ -glucuronidase productivity of intestinal microflora, on t-BHP- or CCl $_4$ -induced hepatotoxicity of mice were evaluated. These oral administration of lactic acid bacteria lowered $\beta$ -glucuronidase production of intestinal microflora as well as Escherichia coli HGU-3. When lactic acid bacteria at a dose of 0.5 or 2 g (wet weight)/kg was orally administered on CCl $_4$ -induced liver injury in mice, these bacteria significantly inhibited the increase of plasma alanine transferase and aspartate transferase activities by $17-57\%$ and $57-66\%$ of the $CCI_4$ control group, respectively. These lactic acid bacteria also showed the potent hepatoprotective effect against t-BHP-induced liver injury in mice. The inhibitory effects of these lactic acid bacteria were more potent than that of dimethyl diphenyl bicarboxylate (DDB), which have been used as a commercial hepatoprotective agent. Among these lactic acid bacteria, L. acidophilus CSG exhibited the most potent hepatoprotective effect. Based on these findings, we insist that an inhibitor of $\beta$ -glucuronidase production in intestine, such as lactic acid bacteria, may be hepatoprotective.

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  6. [국내논문]   Effect of a New Hepatoprotective Agent, YH-439, on the Hepatobiliary Transport of Organic Cations (OCs): Selective Inhibition of Sinusoidal OCs Uptake without Influencing Glucose Uptake and Canalicular OCs Excretion  

    Hong Soon Sun (Department of Pharmaceutics, College of Pharmacy, Seoul National University ) , Li Hong (Department of Pharmaceutics, College of Pharmacy, Seoul National University ) , Choi Min Koo (Department of Pharmaceutics, College of Pharmacy, Seoul National University ) , Chung Suk Jae (Department of Pharmaceutics, College of Pharmacy, Seoul National University ) , Shim Chang Koo (Department of Pharmaceutics, College of Pharmacy, Seoul National University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 330 - 334 , 2005 , 0253-6269 ,

    초록

    The effect of a new hepatoprotective agent, YH-439, on the hepatobiliary transport of a model organic cation (OC), TBuMA (tributylmethylammonium), was investigated. The area under the plasma concentration-time curve (AUC) from time zero to 4 h following iv administration of TBuMA (6.6 $\mu$ mol/kg) was increased significantly when YH-439 in corn oil (300 mg/kg) was orally administered to rats 24 h prior to the experiment. Nevertheless, the cumulative biliary excretion of TBuMA remained unchanged. As a consequence, the apparent biliary clearance ( $CL_b$ ) of TBuMA was decreased significantly as a result of YH-439 pretreatment, consistent with the fact that the in vivo excretion clearance of TBuMA across the canalicular membrane ( $CL_{exc}$ ) was not changed by the pretreatment. The in vitro uptake of TBuMA into isolated hepatocytes was decreased by one half by the pretreatment, owing to a decrease in the apparent V $_{max}$ and $CL_{linear}$ , but the $K_m$ for the process remained constant. Most interestingly, however, the sinusoidal uptake of glucose, a nutrient, into hepatocytes was not influenced by the pretreatment, suggesting the YH-439 pretreatment specifically impaired the sinusoidal uptake of OCs. Thus, the OC-specific inhibition of hepatic uptake, without influencing the uptake of glucose, a nutrient, appeared to be associated with the hepatoprotective activity of YH-439.

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  7. [국내논문]   Memory enhancing and neuroprotective effects of selected ginsenosides  

    Bao, Hai Ying , Zhang, Jing , Yeo, Soo Jeong , Myung, Chang-Seon , Kim, Hyang Mi , Kim, Jong Moon , Park, Jeong Hill , Cho, Jungsook , Kang, Jong Seong
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 335 - 342 , 2005 , 0253-6269 ,

    초록

    The effect of a new hepatoprotective agent, YH-439, on the hepatobiliary transport of a model organic cation (OC), TBuMA (tributylmethylammonium), was investigated. The area under the plasma concentration-time curve (AUC) from time zero to 4 h following iv administration of TBuMA (6.6 $\mu$ mol/kg) was increased significantly when YH-439 in corn oil (300 mg/kg) was orally administered to rats 24 h prior to the experiment. Nevertheless, the cumulative biliary excretion of TBuMA remained unchanged. As a consequence, the apparent biliary clearance ( $CL_b$ ) of TBuMA was decreased significantly as a result of YH-439 pretreatment, consistent with the fact that the in vivo excretion clearance of TBuMA across the canalicular membrane ( $CL_{exc}$ ) was not changed by the pretreatment. The in vitro uptake of TBuMA into isolated hepatocytes was decreased by one half by the pretreatment, owing to a decrease in the apparent V $_{max}$ and $CL_{linear}$ , but the $K_m$ for the process remained constant. Most interestingly, however, the sinusoidal uptake of glucose, a nutrient, into hepatocytes was not influenced by the pretreatment, suggesting the YH-439 pretreatment specifically impaired the sinusoidal uptake of OCs. Thus, the OC-specific inhibition of hepatic uptake, without influencing the uptake of glucose, a nutrient, appeared to be associated with the hepatoprotective activity of YH-439.

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  8. [국내논문]   Memory Enhancing and Neuroprotective Effects of Selected Ginsenosides   피인용횟수: 16

    Sao Hai Ying (College of Medicinal Material, Jilin Agricultural University ) , Zhang Jing (College of Medicinal Material, Jilin Agricultural University ) , Yeo Soo Jeong (College of Pharmacy, Chungnam National University ) , Myung Chang Seon (College of Pharmacy, Chungnam National University ) , Kim Hyang Mi (College of Medicine, Dongguk University ) , Kim Jong Moon (Central Research Institute, Iisung Pharmaceutical Co. ) , Park Jeong Hill (College of Pharmacy, Seoul National University ) , Cho Jung Sook (College of Medicine, Dongguk University ) , Kang Jong Seong (College of Pharmacy, Chungnam National University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 335 - 342 , 2005 , 0253-6269 ,

    초록

    The effects of ginsenosides Rg $_3$ (R) , Rg $_3$ (S) and Rg $_5$ /Rk $_1$ (a mixture of Rg $_5$ and Rk $_1$ 1:1, w/w), which are components isolated from processed Panax ginseng C.A. Meyer (Araliaceae), on memory dysfunction were examined in mice using a passive avoidance test. The ginsenosides Rg3(R), Rg3(S) or Rg $_5$ /Rk $_1$ , when orally administered for 4 days, significantly ameliorated the memory impairment induced by the single oral administration of ethanol. The memory impairment induced by the intraperitoneal injection of scopolamine was also significantly recovered by ginsenosides Rg3(S) and Rg $_5$ /Rk $_1$ . Among the three ginsenosides tested in this study, Rg $_5$ /Rk $_1$ enhanced the memory function of mice most effectively in both the ethanol­and scopolamine-induced amnesia models. Moreover, the latency period of the Rg $_5$ /Rk $_1$ ­treated mice was 1.2 times longer than that of the control (no amnesia) group in both models, implying that Rg $_5$ /Rk $_1$ may also exert beneficial effects in the normal brain. We also evaluated the effects of these ginsenosides on the excitotoxic and oxidative stress-induced neuronal cell damage in primary cultured rat cortical cells. The excitotoxicity induced by glutamate or N­methyl-D-aspartate (NMDA) was dramatically inhibited by the three ginsenosides. Rg $_3$ (S) and Rg $_5$ /Rk $_1$ exhibited a more potent inhibition of excitotoxicity than did Rg $_3$ (R). In contrast, these ginsenosides were all ineffective against the H $_2$ O $_2$ - or xanthine/xanthine oxidase-induced oxidative neuronal damage. Taken together, these results indicate that ginsenosides Rg $_3$ (S) and Rg $_5$ /Rk $_1$ significantly reversed the memory dysfunction induced by ethanol or scopolamine, and their neuroprotective actions against excitotoxicity may be attributed to their memory enhancing effects.

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  9. [국내논문]   Ginsan Improved Th1 Immune Response Inhibited by Gamma Radiation   피인용횟수: 11

    Han Seon Kyu (Laboratory of Immunology, Korea Institute of Radiological and Medical Sciences, Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology ) , Song Jie Young (Laboratory of Immunology, Korea Institute of Radiological and Medical Sciences ) , Yun Yeon Sook (Laboratory of Immunology, Korea Institute of Radiological and Medical Sciences ) , Yi Seh Yoon (Technical Research Institute, Microbia Corp, College of Natural Science, Suwon University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 343 - 350 , 2005 , 0253-6269 ,

    초록

    Gamma radiation causes suppression of the immune function, and immune properties are related to cytokine production. In the present study, the polysaccharide, Ginsan, purified from an ethanol-insoluble fraction of Ginseng (Panax ginseng C.A. Meyer, Araliaceae) water extract was studied to assess its effects on the immunosuppressive activities of gamma radiation. Gin­san was found to stimulate murine normal splenocytes by inducing the mRNA expressions of Th1 and Th2 type cytokines, and also restore the mRNA expression of IFN- $/gamma$ , Th1 cytokine, after its inhibition by whole-body gamma irradiation. Therefore, Ginsan was found to restore the T lymphocytes function that had been suppressed by gamma irradiation in allogenic MLR (mixed lymphocyte reactions). However, Ginsan exhibited no excessive stimulatory effects on the control group. The above results indicated that Ginsan may constitute a new noble agent for the improvement of gamma radiation-induced immunosuppression.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  10. [국내논문]   TERT mRNA Expression is Up-Regulated in MCF-7 Cells and a Mouse Mammary Organ Culture (MMOC) System by Endosulfan Treatment  

    Je Kang Hoon (Natural Products Research Institute, Seoul National University ) , Kim Ki Nam (Natural Products Research Institute, Seoul National University ) , Nam Kung Woo (Natural Products Research Institute, Seoul National University ) , Cho Myung Haing (NIAST, RDA and College of Veterinary Medicine, Seoul National University ) , Mar Woong Chon (Natural Products Research Institute, Seoul National University, College of Pharmacy, Seoul National University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.3 ,pp. 351 - 357 , 2005 , 0253-6269 ,

    초록

    Endosulfan is one of the organochlorine pesticides, which are well-known endocrine disruptors (EDs), and it acts as an estrogen agonist. Estrogen is a group of hormones that play an important role in mammary gland function and are implicated in mammary carcinogenesis. In the present study, we studied the effects of endosulfan on nodule like alveolar lesion (NLAL) formation in mouse mammary gland development using a mouse mammary gland organ culture (MMOC) system. Although endosulfan-treated mammary glands did not form NLALs, more alveolar buds were formed in this group than in the negative control (vehicle-treated) group. In addition, telomerase reverse transcriptase (TERT) mRNA expression levels were increased in endosulfan-treated mammary glands in a dose-dependent manner. Telomerase can be activated by estrogen, therefore, we examined the effects of endosulfan on telomerase activity, and found that the telomerase activity in estrogen receptor-positive MCF-7 cells was up-regulated by endosulfan treatment. Moreover, this activation was accompanied by the up­regulation of the TERT mRNA expression. Also, transient expression assays using CAT reporter plasm ids containing various fragments of the TERT promoter showed that this imperfect palindromic estrogen-responsive element is almost certainly responsible for the transcriptional activation by endosulfan. These results may help elucidate the endocrine disrupting mechanism of endosulfan.

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