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Archives of pharmacal research : a publication of ... 25건

  1. [국내논문]   Role of Disulfide Bond of Arylsulfate Sulfotransferase in the Catalytic Activity  

    Kwon, Ae-Ran (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University ) , Choi, Eung-Chil (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 561 - 565 , 2005 , 0253-6269 ,

    초록

    Bacterial arylsulfate sulfotransferase (ASST) catalyzes the transfer of sulfate group from a phenyl sulfate ester to a phenolic acceptor. The promoter region and the transcripti on start sites of Enterobacter amnigenus astA have been determined by primer extension analysis. Northern blot analysis resolved two mRNA species with lengths of 3.3 and 2.0 kb, which correspond to the distances between the transcriptional initiation sites and the two inverted repeat sequences (IRSs). By length, the 3.3 kb RNA could comprise the three-gene (astA with dsbA and dsbB) operon. ASST has three highly conserved cysteine residues. Reducing and non-reducing SDS-PAGE and activity staining showed that disulfide bond is needed for the activity of the enzyme. To identify the cysteine residues responsible for the disulfide bond formation, a series of Cys to Ser mutants has been constructed and the enzymatic activity was measured. Based on the results, we assumed that the first cysteine (Cys349) might be involved in disulfide bond mainly with the second cysteine (Cys445) and result in active conformation.

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  2. [국내논문]   Improvement of Menopausal Signs by Isoflavones Derived from Sophorae fructus in Ovariectomized Female Rats and the Antioxidant Potentials in BV2 Cells   피인용횟수: 2

    Joo, Seong-Soo (College of Pharmacy, Chung-Ang University ) , Kwon, Suk-Hyung (Institute for Life Science, Rexgene Biotech ) , Hwang, Kwang-Woo (College of Pharmacy, Chung-Ang University ) , Lee, Do-Ik (College of Pharmacy, Chung-Ang University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 566 - 572 , 2005 , 0253-6269 ,

    초록

    The aim of this study was to determine if the isoflavones from Sophorae fructus (SISO) have potential clinical benefit in hormone replacement therapy (HRT) for the treat ment of menopausal signs, such as the levels of total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and follicle stimulating hormone (FSH). An additional aim was to present the potential antioxidant effect of SISO in a microglial cell line. For the animal model, the ovaries were removed from adult rats and the indicators of menopause were measured at the pre- and post-administration time points. Although no statistically significant correlation was found, SISO tended to decrease the TC level (p=0.15) and the FSH level (p=0.36), but to increase the HDL level (p=0.303).SISO ( ${\mu}g$ /mL) also exerted antioxidant activity on BV-2 microglial cells by inhibiting lipopolysaccharide-induced nitric oxide. This cytoprotective effect was confirmed by trypan blue staining, which was used to test for cellular damage from H $_2O_2$ . In conclusion, this study highlights the anti-menopausal and antioxidant effect of SISO in an ovariectomized rat model, as well as in microglial cells, and provides new clinical targets for the screening of phytoestrogens as potential candidates for HRT in menopausal women.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  3. [국내논문]   Production of Cytokine and NO by RAW 264.7 Macrophages and PBMC In Vitro Incubation with Flavonoids  

    Lyu, Su-Yun , Park, Won-Bong
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 573 - 581 , 2005 , 0253-6269 ,

    초록

    The aim of this study was to determine if the isoflavones from Sophorae fructus (SISO) have potential clinical benefit in hormone replacement therapy (HRT) for the treat ment of menopausal signs, such as the levels of total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and follicle stimulating hormone (FSH). An additional aim was to present the potential antioxidant effect of SISO in a microglial cell line. For the animal model, the ovaries were removed from adult rats and the indicators of menopause were measured at the pre- and post-administration time points. Although no statistically significant correlation was found, SISO tended to decrease the TC level (p=0.15) and the FSH level (p=0.36), but to increase the HDL level (p=0.303).SISO ( ${\mu}g$ /mL) also exerted antioxidant activity on BV-2 microglial cells by inhibiting lipopolysaccharide-induced nitric oxide. This cytoprotective effect was confirmed by trypan blue staining, which was used to test for cellular damage from H $_2O_2$ . In conclusion, this study highlights the anti-menopausal and antioxidant effect of SISO in an ovariectomized rat model, as well as in microglial cells, and provides new clinical targets for the screening of phytoestrogens as potential candidates for HRT in menopausal women.

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  4. [국내논문]   Prosuction of Cytokine and NO by RAW 264.7 Macrophages and PBMC In Vitro Incubation with Flavonoids  

    Lyu, Su-Yun (Immune Modulation Research Laboratory, The Boots Science Building, School of Pharmacy, University of Nottingham ) , Park, Won-Bong (College of Natural Sciences, Seoul Women's University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 573 - 581 , 2005 , 0253-6269 ,

    초록

    Flavonoids, a group of low molecular weight phenylbenzopyrones, have various pharmacological properties including antioxidant activity, anticancer, and immunomodulatory effects. In the present study, lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate/phytohemagglutinin (PMA/PHA) were used as stimulants for RAW 264.7 macrophages and human peripheral blood mononuclear cell (hPBMC), and tumor necrosis factor (TNF)- ${\alpha}$ and interleukin (IL)-2 productions were measured. In addition, flavonoids were examined for their effects on LPS-induced NO production in RAW 264.7 macrophages. The results showed that all compounds were not strongly cytotoxic at the tested concentrations on hPBMC and RAW 264.7 macrophages. On immunomodulatory properties, catechin, epigallocatechin (EGC), naringenin, and fisetin repressed NO production and TNF- ${\alpha}$ secretion. Furthermore, catechin, epigallocatechin gallate (EGCG), epicatechin (EC), luteolin, chrysin, quercetin, and galangin increased IL-2 secretion while EGC, apigenin, and fisetin inhibited the secretion. These results indicated that flavonoids have the capacity to modulate the immune response and have a potential anti-inflammatory activity. There was no obvious structure-activity relationship regard to the chemical composition of the flavonoids and their cell biological effects.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [국내논문]   Differential Modulatory Effects of Cholera Toxin and Pertussis Toxin on Pain Behavior Induced by TNF-a, lnterleukin-1β and Interferon- Injected Intrathecally  

    Kwon, Min-Soo , Shim, Eon-Jeong , Seo, Young-Jun , Choi, Seong-Soo , Lee, Jin-Young , Lee, Han-Kyu , Suh, Hong-Won
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 582 - 586 , 2005 , 0253-6269 ,

    초록

    Flavonoids, a group of low molecular weight phenylbenzopyrones, have various pharmacological properties including antioxidant activity, anticancer, and immunomodulatory effects. In the present study, lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate/phytohemagglutinin (PMA/PHA) were used as stimulants for RAW 264.7 macrophages and human peripheral blood mononuclear cell (hPBMC), and tumor necrosis factor (TNF)- ${\alpha}$ and interleukin (IL)-2 productions were measured. In addition, flavonoids were examined for their effects on LPS-induced NO production in RAW 264.7 macrophages. The results showed that all compounds were not strongly cytotoxic at the tested concentrations on hPBMC and RAW 264.7 macrophages. On immunomodulatory properties, catechin, epigallocatechin (EGC), naringenin, and fisetin repressed NO production and TNF- ${\alpha}$ secretion. Furthermore, catechin, epigallocatechin gallate (EGCG), epicatechin (EC), luteolin, chrysin, quercetin, and galangin increased IL-2 secretion while EGC, apigenin, and fisetin inhibited the secretion. These results indicated that flavonoids have the capacity to modulate the immune response and have a potential anti-inflammatory activity. There was no obvious structure-activity relationship regard to the chemical composition of the flavonoids and their cell biological effects.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  6. [국내논문]   Differential Modulatory Effects of Cholera Toxin and Pertussis Toxin on Pain Behavior Induced by TNF-${\alpha}$, Interleukin-1${\beta}$ and Interferon-${\gamma}$ Injected Intrathecally   피인용횟수: 1

    Kwon, Min-Soo (Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University ) , Shim, Eon-Jeong (Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University ) , Seo, Young-Jun (Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University ) , Choi, Seong-Soo (Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University ) , Lee, Jin-Young (Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University ) , Lee, Han-Kyu (Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University ) , Suh, Hong-Won (Department of Pharmacology, College of Medicine, and Institute of Natural Medicine, Hallym University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 582 - 586 , 2005 , 0253-6269 ,

    초록

    The present study was designed to characterize the possible roles of spinally located cholera toxin (CTX)- and pertussis toxin (PTX)-sensitive G-proteins in pro- inflammatory cy tokine induced pain behaviors. Intrathecal injection of tumor necrosis factor-a (TNF- ${\alpha}$ ; 100 pg), interleukin-1 ${\beta}$ (IL-1 ${\beta}$ 100 pg) and interferon- ${\gamma}$ (INF- ${\gamma}$ ; 100 pg) showed pain behavior. Intrathecal pretreatment with CTX (0.05, 0.1 and 0.5 mg) attenuated pain behavior induced by TNF- ${\alpha}$ and INF- ${\gamma}$ administered intrathecally. But intrathecal pretreatment with CTX (0.05, 0.1 and 0.5 ${\mu}g$ ) did not attenuate pain behavior induced by IL-1 ${\beta}$ . On the other hand, intrathecal pretreatment with PTX further increased the pain behavior induced by TNF- ${\alpha}$ and IL-1 ${\beta}$ administered intrathecally, especially at the dose of 0.5 ${\mu}g$ . But intrathecal pretreatment with PTX did not affect pain behavior induced by INF- ${\gamma}$ . Our results suggest that, at the spinal cord level, CTX- and PTX-sensitive G-proteins appear to play important roles in modulating pain behavior induced by pro-inflammatory cytokines administered spinally. Furthermore, TNF- ${\alpha}$ , IL-1 ${\beta}$ arid INF- ${\gamma}$ administered spinally appear to produce pain behavior by different mechanisms.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  7. [국내논문]   Extremely Low Frequency Magnetic Fields Modulate Bicuculline-lnduced-Convulsion in Rats  

    Jeong, Ji Hoon , Choi, Kyung Bum , Choi, Hee Jung , Song, Hyun Ju , Min, Young Sil , Ko, Sung Kwon , Im, Byung Ok , Sohn, Uy Dong
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 587 - 591 , 2005 , 0253-6269 ,

    초록

    The present study was designed to characterize the possible roles of spinally located cholera toxin (CTX)- and pertussis toxin (PTX)-sensitive G-proteins in pro- inflammatory cy tokine induced pain behaviors. Intrathecal injection of tumor necrosis factor-a (TNF- ${\alpha}$ ; 100 pg), interleukin-1 ${\beta}$ (IL-1 ${\beta}$ 100 pg) and interferon- ${\gamma}$ (INF- ${\gamma}$ ; 100 pg) showed pain behavior. Intrathecal pretreatment with CTX (0.05, 0.1 and 0.5 mg) attenuated pain behavior induced by TNF- ${\alpha}$ and INF- ${\gamma}$ administered intrathecally. But intrathecal pretreatment with CTX (0.05, 0.1 and 0.5 ${\mu}g$ ) did not attenuate pain behavior induced by IL-1 ${\beta}$ . On the other hand, intrathecal pretreatment with PTX further increased the pain behavior induced by TNF- ${\alpha}$ and IL-1 ${\beta}$ administered intrathecally, especially at the dose of 0.5 ${\mu}g$ . But intrathecal pretreatment with PTX did not affect pain behavior induced by INF- ${\gamma}$ . Our results suggest that, at the spinal cord level, CTX- and PTX-sensitive G-proteins appear to play important roles in modulating pain behavior induced by pro-inflammatory cytokines administered spinally. Furthermore, TNF- ${\alpha}$ , IL-1 ${\beta}$ arid INF- ${\gamma}$ administered spinally appear to produce pain behavior by different mechanisms.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  8. [국내논문]   Extremely Low Frequency Magnetic Fields Modulate Bicuculline-Induced-Convulsion in Rats  

    Jeong, Ji-Hoon (Department of Pharmacology, College of Medicine, Department of Pharmacology, College of Pharmacy, Chung Ang University ) , Choi, Kyung-Bum (Department of Pharmacology, College of Pharmacy, Chung Ang University ) , Choi, Hee-Jung (Department of Pharmacology, College of Pharmacy, Chung Ang University ) , Song, Hyun-Ju (Department of Pharmacology, College of Pharmacy, Chung Ang University ) , Min, Young-Sil (Department of Pharmacology, College of Pharmacy, Chung Ang University ) , Ko, Sung-Kwon (Korea Ginseng Institute, Chung Ang University ) , Im, Byung-Ok (Korea Ginseng Institute, Chung Ang University ) , Sohn, Uy-Dong (Department of Pharmacology, College of Pharmacy, Chung Ang University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 587 - 591 , 2005 , 0253-6269 ,

    초록

    The effect of extremely low frequency (ELF,60Hz) magnetic fields (MFs) on convulsions was investigated in rats. We determined the onset arid duration of convulsions induced by bicuculline alone or by co-exposure to MFs and bicuculline. In addition, we measured the GABA concentrations in the rat brains using HPLC-ECD. MFs strengthened the convulsion induced by bicuculline (0.3, 1, and 3 ${\mu}g$ , I.c.v.), with a shortening of the onset time, but lengthening of the duration time. Co-exposure to MFs and bicuculline decreased the GABA levels in the cortex, hippocampus and hypothalamus, whereas MFs alone reduced the level of GABA only in the hippocampus. These results suggest that the exposure to MFs may modulate bicuculline-induced convulsions due to GABA neurotransmissions in rat brains.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

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  9. [국내논문]   Identification And Quantification of Steroidal Saponins in Polygonatum Species by HPLC/ESI/MS  

    Ahn, Mi-Jeong (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University ) , Kim, Jin-Woong (College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University)
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 592 - 597 , 2005 , 0253-6269 ,

    초록

    An HPLC/Esl/MS method has been developed to identify and quantify the spirostanol glycosides in the rhizomes of five Polygonatum species. The relative distribution of two steroidal saponins in each extract was established using the selective ion monitoring (SIM) mode via an electrospray ionization (ESI) source. It was found that there were significant differences in the amount of spirostanol glycosides among the Polygonatum Species. The results showed that this method could be used to identify the steroidal saponins in the extracts and differentiate Polygonatum species with high sensitivity and reproducibility in a short time. Fragmentation patterns of the two reference compounds were also discussed with the electrospray ionization multi-stage tandem mass spectroscopy (ESI-MS $^n$ ).

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  10. [국내논문]   The influence of assay error weight on gentamicin pharmacokinetics using the bayesian and nonlinear least square regression analysis in appendicitis patients  

    Burm, Jin Pil
    Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea v.28 no.5 ,pp. 598 - 603 , 2005 , 0253-6269 ,

    초록

    An HPLC/Esl/MS method has been developed to identify and quantify the spirostanol glycosides in the rhizomes of five Polygonatum species. The relative distribution of two steroidal saponins in each extract was established using the selective ion monitoring (SIM) mode via an electrospray ionization (ESI) source. It was found that there were significant differences in the amount of spirostanol glycosides among the Polygonatum Species. The results showed that this method could be used to identify the steroidal saponins in the extracts and differentiate Polygonatum species with high sensitivity and reproducibility in a short time. Fragmentation patterns of the two reference compounds were also discussed with the electrospray ionization multi-stage tandem mass spectroscopy (ESI-MS $^n$ ).

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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