본문 바로가기
HOME> 저널/프로시딩 > 저널/프로시딩 검색상세

저널/프로시딩 상세정보

권호별목차 / 소장처보기

H : 소장처정보

T : 목차정보

Journal of clinical pathology 17건

  1. [해외논문]   DNA hypermethylation analysis in sputum of asymptomatic subjects at risk for lung cancer participating in the NELSON trial: argument for maximum screening interval of 2 years   SCI SCIE

    Hubers, A Jasmijn (Department of Pathology, VU University Medical Center, , Amsterdam, The Netherlands ) , Heideman, Danielle A M (Department of Pathology, VU University Medical Center, , Amsterdam, The Netherlands ) , Duin, Sylvia (Department of Pathology, VU University Medical Center, , Amsterdam, The Netherlands ) , Witte, Birgit I (Department of Epidemiology and Biostatistics, VU University Medical Center, , Amsterdam, The Netherlands ) , de Koning, Harry J (Department of Public Health, Erasmus Medical Center, , Rotterdam, The Netherlands ) , Groen, Harry J M (Department of Pulmonary Diseases, University Medical Center Groningen, , Groningen, The Netherlands ) , Prinsen, Clemens F M (Department of Pathology, Canisius-Wilhelmina Hospital, , Nijmegen, The Netherlands ) , Bolijn, Anne S (Department of Pathology, VU University Medical Center, , Amsterdam, The Netherlands ) , Wouters, Mandy (Department of Pathology, Canisius-Wilhelmina Hospital, , Nijmegen, The Netherlands ) , van der Meer, Susanne E (Department of Pathology, Canisius-Wilhelmina Hospital, , Nijmegen, The Netherlands ) , Steenbergen, Renske D M (Department of Pathology, VU University Medical Center, , Amsterdam, The Netherlands ) , Snijders, Peter J F (Department of Patholog) , Uyterlinde, Anne , Berkhof, Hans , Smit, Egbert F , Thunnissen, Erik
    Journal of clinical pathology v.70 no.3 ,pp. 250 - 254 , 2017 , 0021-9746 ,

    초록

    Aims Lung cancer is the major contributor to cancer mortality due to metastasised disease at time of presentation. The current study investigated DNA hypermethylation of biomarkers RASSF1A , APC , cytoglobin, 3OST2, FAM19A4, PHACTR3 and PRDM14 in sputum of asymptomatic high-risk individuals from the NELSON lung cancer low-dose spiral CT screening trial to detect lung cancer at preclinical stage. Methods Subjects were selected with (i) lung cancer in follow-up (cases; n=65), (ii) minor cytological aberrations (controls; n=120) and (iii) a random selection of subjects without cytological aberrations (controls; n=99). Median follow-up time for controls was 80 months. Cut-off values were based on high specificity to assess diagnostic value of the biomarkers. Results RASSF1A may denote presence of invasive cancer because of its high specificity (93% (95% CI 89% to 96%); sensitivity 17% (95% CI 4% to 31%), with best performance in a screening interval of 2 years. The panel of RASSF1A, 3OST2 and PRDM14 detected 28% (95% CI 11% to 44%) of lung cancer cases within 2 years, with specificity of 90% (95% CI 86% to 94%). Sputum cytology did not detect any lung cancers. Conclusions In a lung cancer screening setting with maximum screening interval of 2 years, DNA hypermethylation analysis in sputum may play a role in the detection of preclinical disease, but complementary diagnostic markers are needed to improve sensitivity.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  2. [해외논문]   Comprehensive profiling of metaplastic breast carcinomas reveals frequent overexpression of programmed death-ligand 1   SCI SCIE

    Joneja, Upasana (Department of Pathology, Anatomy and Cell Biology , Thomas Jefferson University Hospital , Philadelphia, Pennsylvania , USA ) , Vranic, Semir (Department of Pathology , University Clinical Center Sarajevo , Sarajevo , Bosnia and Herzegovina ) , Swensen, Jeffrey (Caris Life Sciences , Phoenix, Arizona , USA ) , Feldman, Rebecca (Caris Life Sciences , Phoenix, Arizona , USA ) , Chen, Wangjuh (Caris Life Sciences , Phoenix, Arizona , USA ) , Kimbrough, Jeffrey (Caris Life Sciences , Phoenix, Arizona , USA ) , Xiao, Nianqing (Caris Life Sciences , Phoenix, Arizona , USA ) , Reddy, Sandeep (Caris Life Sciences , Phoenix, Arizona , USA ) , Palazzo, Juan (Department of Pathology, Anatomy and Cell Biology , Thomas Jefferson University Hospital , Philadelphia, Pennsylvania , USA ) , Gatalica, Zoran (Caris Life Sciences , Phoenix, Arizona , USA )
    Journal of clinical pathology v.70 no.3 ,pp. 255 - 259 , 2017 , 0021-9746 ,

    초록

    Aims Metaplastic breast carcinoma (MBC) is a rare subtype of breast carcinoma less responsive to conventional chemotherapy than ductal carcinoma. In molecular terms, MBCs usually cluster with triple-negative breast cancers (TNBCs), but have a worse prognosis than TNBCs. Studies investigating MBCs for specific biomarkers of therapy response are rare and limited by the methodological approaches. The aim of the present study was to characterise MBCs on a molecular level and test programmed death-ligand 1 (PD-L1) biomarker expression in MBCs for future therapeutic interventions. Methods We profiled 297 samples (MBC (n=75), TNBC (n=106), human epidermal growth factor receptor 2 (HER2)-positive breast cancers (n=32) and hormone-positive breast cancers (n=84)) by next-generation sequencing. Immunohistochemistry for PD-L1 and programmed cell death 1 (PD-1) expression was performed using automated procedures. Results The most commonly mutated genes in MBCs included TP53 (56%) and PIK3CA (23%). Pathogenic mutations in other genes, including HRAS , FBXW7 , PTEN , AKT1 and SMAD4 , were rare. PD-L1 expression was detected in a significantly higher proportion of MBCs (46%) than in other subtypes (6% each in hormone-positive and HER2-positive breast cancers, and 9% in TNBC, not otherwise specified, p Conclusions Comprehensive profiling of a large cohort of this rare subtype of breast carcinoma highlighted the predominance of TP53 mutation and increased PD-L1 expression in carcinoma cells. These results can be exploited in clinical trials using immune checkpoint inhibitors.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  3. [해외논문]   The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing   SCI SCIE

    Yeo, Min-Kyung (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea ) , Jung, Min-Kyu (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea ) , Lee, Su-Yel (Biobank of Chungnam National University Hospital, , Daejeon, Korea ) , Lee, Yong-Moon (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea ) , Hur, Gang Min (Department of Pharmacology, Chungnam National University School of Medicine, , Daejeon, Korea ) , Kim, Jin-Man (Department of Pathology and Medical Science, Chungnam National University School of Medicine, , Daejeon, Korea)
    Journal of clinical pathology v.70 no.3 ,pp. 260 - 265 , 2017 , 0021-9746 ,

    초록

    Introduction The BRAF V600E mutation is the most common genetic event in papillary thyroid carcinoma (PTC). The BRAF V600E mutational status has a significant diagnostic and prognostic role in PTC since it can be detected in 32%-87% of PTC by various molecular methods. Aim(s) A novel, fully automated real-time PCR-based Idylla test is assessed to detect the BRAF mutation in formalin-fixed paraffin-embedded (FFPE) thyroid samples. Methods 99 PTC and 11 nodular hyperplasia FFPE thyroid tissues are evaluated for the BRAF V600E mutation by the Idylla tests and compared with peptide nucleic acid-clamping PCR, real-time PCR and pyrosequencing. Results The sensitivity and specificity of the Idylla test to detect BRAF V600E are 98.8% and 100%, which is superior to real-time PCR and pyrosequencing. The concordance between Idylla and true positive is highest at 0.974. Conclusions This study validates that the Idylla test is a sensitive and specific method to detect BRAF V600E in FFPE thyroid tissues. A simple, quick and easy to handle Idylla test is a useful and reliable molecular technique to evaluate BRAF mutations.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  4. [해외논문]   Correction   SCI SCIE


    Journal of clinical pathology v.70 no.3 ,pp. 265 - 265 , 2017 , 0021-9746 ,

    초록

    Introduction The BRAF V600E mutation is the most common genetic event in papillary thyroid carcinoma (PTC). The BRAF V600E mutational status has a significant diagnostic and prognostic role in PTC since it can be detected in 32%-87% of PTC by various molecular methods. Aim(s) A novel, fully automated real-time PCR-based Idylla test is assessed to detect the BRAF mutation in formalin-fixed paraffin-embedded (FFPE) thyroid samples. Methods 99 PTC and 11 nodular hyperplasia FFPE thyroid tissues are evaluated for the BRAF V600E mutation by the Idylla tests and compared with peptide nucleic acid-clamping PCR, real-time PCR and pyrosequencing. Results The sensitivity and specificity of the Idylla test to detect BRAF V600E are 98.8% and 100%, which is superior to real-time PCR and pyrosequencing. The concordance between Idylla and true positive is highest at 0.974. Conclusions This study validates that the Idylla test is a sensitive and specific method to detect BRAF V600E in FFPE thyroid tissues. A simple, quick and easy to handle Idylla test is a useful and reliable molecular technique to evaluate BRAF mutations.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  5. [해외논문]   A simple human co-culture model of lymphatic invasion   SCI SCIE

    Naidoo, Kalnisha (Guy's and St Thomas' NHS Foundation Trust, , London, UK)
    Journal of clinical pathology v.70 no.3 ,pp. 266 - 269 , 2017 , 0021-9746 ,

    초록

    True lymphovascular invasion in most carcinomas adversely affects survival, but in vitro human models of lymphatic invasion are lacking. Herein is discussed one such model in which certain mechanistic aspects of lymphatic dissemination can be investigated easily in any laboratory. S100P expression is known to be significantly upregulated in the lymph node metastases of human pancreatic adenocarcinoma. Thus, an S100P overexpressing cell line (S5L) and a vector control (V3L) pancreatic cancer cell lines were used to develop a co-culture model with human dermal lymphatic endothelial cells. Adhesion, the effect of cancer cells on lymphatic permeability and trans-lymphatic endothelial migration could be reproducibly quantified in this model and parametric statistical analysis confirmed significant differences between the two cancer cell lines in these functional assays. This proof-of-principle study demonstrates that this simple model can be used to assess lymphatic invasion in a human cancer context in real-time in vitro.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  6. [해외논문]   Incidental EBV-positivity in paediatric post-transplant specimens demonstrates the need for stringent criteria for diagnosing post-transplant lymphoproliferative disorders   SCI SCIE

    King, Rebecca L (Division of Hematopathology, Mayo Clinic, , Rochester, Minnesota, USA ) , Paessler, Michele E (Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, , Philadelphia, Pennsylvania, USA ) , Howard, Matthew T (Division of Hematopathology, Mayo Clinic, , Rochester, Minnesota, USA ) , Wertheim, Gerald B (Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, , Philadelphia, Pennsylvania, USA)
    Journal of clinical pathology v.70 no.3 ,pp. 270 - 273 , 2017 , 0021-9746 ,

    초록

    Aims To examine the need for minimal diagnostic criteria for post-transplant lymphoproliferative disorders (PTLD) in children, we sought to determine the rate of incidental Epstein-Barr virus (EBV)-positivity in tissues from organ transplant recipients (OTR). Methods EBV in situ hybridisation (ISH) was done retrospectively on tissue from 34 paediatric autopsies of OTR and paediatric tonsillectomy specimens from non-OTR (96) and OTR (6). Patients with a history of PTLD were excluded from both data sets. Results EBV-positivity was found incidentally in 2/34 autopsy cases (5.9%). Median time from transplant to death for all patients was 12.8 months (range 0.1–153 months). Median time between transplant and death in EBV-positive cases was 34 months. EBV was positive in 26/102 tonsils (25%). Among tonsils from OTR, 4/6 (67%) were EBV-positive. Conclusions These findings reinforce the need for strict morphological and clinical criteria, other than EBV-positivity, when diagnosing PTLD in the paediatric population.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  7. [해외논문]   Prognostic implications of RASAL1 expression in oesophagogastric adenocarcinoma   SCI SCIE

    Knief, Juliana (Department of Pathology, Section of Hematopathology and endocrine Pathology, University Hospital of Schleswig-Holstein, , Luebeck, Germany ) , Reddemann, Katharina (Department of Pathology, Section of Hematopathology and endocrine Pathology, University Hospital of Schleswig-Holstein, , Luebeck, Germany ) , Lazar-Karsten, Pamela (Department of Pathology, Section of Hematopathology and endocrine Pathology, University Hospital of Schleswig-Holstein, , Luebeck, Germany ) , Herhahn, Tobias (Department of Pathology, Section of Hematopathology and endocrine Pathology, University Hospital of Schleswig-Holstein, , Luebeck, Germany ) , Petrova, Ekaterina (Department of Surgery, University Hospital of Schleswig-Holstein, , Luebeck, Germany ) , Wellner, Ulrich (Department of Surgery, University Hospital of Schleswig-Holstein, , Luebeck, Germany ) , Thorns, Christoph (Department of Pathology, Section of Hematopathology and endocrine Pathology, University Hospital of Schleswig-Holstein, , Luebeck, Germany)
    Journal of clinical pathology v.70 no.3 ,pp. 274 - 276 , 2017 , 0021-9746 ,

    초록

    Aims To examine the need for minimal diagnostic criteria for post-transplant lymphoproliferative disorders (PTLD) in children, we sought to determine the rate of incidental Epstein-Barr virus (EBV)-positivity in tissues from organ transplant recipients (OTR). Methods EBV in situ hybridisation (ISH) was done retrospectively on tissue from 34 paediatric autopsies of OTR and paediatric tonsillectomy specimens from non-OTR (96) and OTR (6). Patients with a history of PTLD were excluded from both data sets. Results EBV-positivity was found incidentally in 2/34 autopsy cases (5.9%). Median time from transplant to death for all patients was 12.8 months (range 0.1–153 months). Median time between transplant and death in EBV-positive cases was 34 months. EBV was positive in 26/102 tonsils (25%). Among tonsils from OTR, 4/6 (67%) were EBV-positive. Conclusions These findings reinforce the need for strict morphological and clinical criteria, other than EBV-positivity, when diagnosing PTLD in the paediatric population.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지

논문관련 이미지