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Journal of microbiology and biotechnology 14건

  1. [국내논문]   Immunosuppressive Characteristics of Oligomycin Derivatives Produced by Streptomyces lydicus MCY-524  

    Lee, Sang-Yong (Department of Microbiology, Kyungpook National University ) , Han, Sang-Bae (Korea Research Institute of Bioscience and Biotechnology, KIST ) , Kim, Hang-Sub (Korea Research Institute of Bioscience and Biotechnology, KIST ) , Kim, Young-Ho (College of Pharmacy, Chungnam National University ) , Kim, Hwan-Mook (Korea Research Institute of Bioscience and Biotechnology, KIST ) , Kim, Chang-Jin (Korea Research Institute of Bioscience and Biotechnology, KIST ) , Hong, Soon-Duck (Department of Microbiology, Kyungpook National University ) , Lee, Jung-Joon (Korea Research Institute of Bioscience and Biotechnology, KIST)
    Journal of microbiology and biotechnology v.7 no.1 ,pp. 56 - 61 , 1997 , 1017-7825 ,

    초록

    A strain producing immunosuppressive substances was isolated from a soil in Cheju island. By morphological, cultural, and physiological studies, the strain was identified as Streptomyces lydicus MCY-524. Cultured broth was purified by silica gel, sephadex LH-20 and preparative HPLC and gave two immunosuppressive compounds, MCH-22 and MCH-32. They dramatically suppressed the B cell activation with lipopolysaccharide, T cell activation by mixed lymphocyte response, and primary T-dependent antibody response at a final concentration of 1 ${\mu}g$ /ml. They also markedly suppressed the proliferation of lymphocytes induced by lipopolysaccharide, pokeweed mitogen, and concanavaline A at the same concentration. Their suppressive activities, which were comparable to those of cyclosporin A, suggested that they were potent and broad immunotoxic agents on the immune functions of murine lymphocytes.

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  2. [국내논문]   Characterization of Ethanol Fermentation Using Alginate Immobilized Thermotolerant Yeast Cells   피인용횟수: 2

    Sohn, Ho-Yong (Department of Microbiology, College of Natural Science, Kyungpook National University ) , Park, Wan (Department of Microbiology, College of Natural Science, Kyungpook National University ) , Jin, Ingnyol (Department of Microbiology, College of Natural Science, Kyungpook National University ) , Seu, Jung-Hwn (Department of Microbiology, College of Natural Science, Kyungpook National University)
    Journal of microbiology and biotechnology v.7 no.1 ,pp. 62 - 67 , 1997 , 1017-7825 ,

    초록

    To enhance the hyperproductive and low energy-consuming ethanol fermentation rate, the thermotolerant yeast S. cerevisiae RA-74-2 cells were immobilized. An efficient immobilization condition was proved to be $1.5{\%}$ (w/v) alginate solution, neutral pH and 20 h activation of beads. The fermentation characteristics and stability at various temperatures were examined as compared with free S. cerevisiae RA-74-2 cells. The immobilized cells had excellent fermentation rate at the range of pH 3-7 at 30- $42^{\circ}C$ in 15- $20{\%}$ glucose media. When the seed volume was adjusted to 0.12 (v/v) (6ml bead/50 ml medium), $11{\%}$ (w/v) ethanol was produced during the first 34 hand $12.15{\%}$ (w/v) ethanol [ $95{\%}$ (w/v) of theoretical yield] during the first 60 h in $25{\%}$ glucose medium. In repetitive fermentation using a 2 litre fermentor, 5.79- $7.27{\%}$ (w/v) ethanol [76- $95{\%}$ (w/v) of theoretical yield] was produced during the 40-55 h in $15{\%}$ glucose media. These data suggested the fact that alginate beads of thermotolerant S. cerevisiae RA-74-2 cells would contribute to economic and hyperproductive ethanol fermentation at high temperature.

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  3. [국내논문]   Classification of Isolates Originating from Kimchi Using Carbon-source Utilization Patterns  

    Lee, J.-S. ; Chun, Chang Ouk ; Jung, M.-C. ; Kim, W.-S. ; Kim, H.-J. ; Hector, M. ; Kim, S.-B. ; Park, C.-S. ; Ahn, J.-S. ; Park, Y.-H.
    Journal of microbiology and biotechnology v.7 no.1 ,pp. 68 - 74 , 1997 , 1017-7825 ,

    초록

    To enhance the hyperproductive and low energy-consuming ethanol fermentation rate, the thermotolerant yeast S. cerevisiae RA-74-2 cells were immobilized. An efficient immobilization condition was proved to be $1.5{\%}$ (w/v) alginate solution, neutral pH and 20 h activation of beads. The fermentation characteristics and stability at various temperatures were examined as compared with free S. cerevisiae RA-74-2 cells. The immobilized cells had excellent fermentation rate at the range of pH 3-7 at 30- $42^{\circ}C$ in 15- $20{\%}$ glucose media. When the seed volume was adjusted to 0.12 (v/v) (6ml bead/50 ml medium), $11{\%}$ (w/v) ethanol was produced during the first 34 hand $12.15{\%}$ (w/v) ethanol [ $95{\%}$ (w/v) of theoretical yield] during the first 60 h in $25{\%}$ glucose medium. In repetitive fermentation using a 2 litre fermentor, 5.79- $7.27{\%}$ (w/v) ethanol [76- $95{\%}$ (w/v) of theoretical yield] was produced during the 40-55 h in $15{\%}$ glucose media. These data suggested the fact that alginate beads of thermotolerant S. cerevisiae RA-74-2 cells would contribute to economic and hyperproductive ethanol fermentation at high temperature.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  4. [국내논문]   Biomass Production of Saccharomyces cerevisiae KFCC 10823 and Its Use in Preparation of Doenjang   피인용횟수: 1

    Yoo, Jin-Young (Food Biotechnology Division, Korea Food Research Institute ) , Kim, Hyeon-Gyu (Food Biotechnology Division, Korea Food Research Institute ) , Kwon, Dong-Jin (Food Biotechnology Division, Korea Food Research Institute)
    Journal of microbiology and biotechnology v.7 no.1 ,pp. 75 - 80 , 1997 , 1017-7825 ,

    초록

    An ethanolic fermentation process was developed for preparing Doenjang with high ethanol. Higher and efficient viable cell production of salt-tolerant ethanolic yeast is a prerequisite for the successful commercial-scale process of ethanol production during Doenjang fermentation. Culture conditions of salt-tolerant yeast, S. cerevisiae KFCC 10823, was studied in terms of the effect of several environmental and nutritional factors. Viable cell numbers were the highest in a medium containing the following components per liter of water: soysauce, 300ml; dextrose, 50 g; beef extract, 5 g; yeast extract, 5 g; $KH_2PO_4$ , 5 g; NaCl, 50 g. The optimal culture conditions of S. cerevisiae KFCC 10823 were pH 5.5, $25^{\circ}C$ , 200 rpm and 0.5 vvm. Yeast viability during batch fermentation was gradually decreased to a level less than $90{\%}$ after 35 hours. The maximum cell number was $2.2{\times}10_7$ cells/ml at the optimal condition. Doenjang prepared with ethanolic yeast was ripened after 45 days at $30^{\circ}C$ . This Doenjang contains 470 mg% of amino nitrogen and 2.5% ethanol. The shelf-life at $30^{\circ}C$ was theoretically estimated as 444 days.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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