본문 바로가기
HOME> 저널/프로시딩 > 저널/프로시딩 검색상세

저널/프로시딩 상세정보

권호별목차 / 소장처보기

H : 소장처정보

T : 목차정보

Journal of bacteriology and virology : JBV 20건

  1. [국내논문]   Host Gene Profiling of Coxsackievirus B3 H3- and 10A1-infected Mouse Heart  

    Nam, Jae-Hwan (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea. ) , Lim, Byung-Kwan (Department of Medicine, Sunkyunkwan University School of Medicine, Cardiac and Vascular Center, Samsung Medical Center, 50 Il-Won Dong, Kangnam-Ku, Seoul 135-710, Korea. ) , Cho, Young-Joo (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea. ) , Kim, Dae-Sun (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea. ) , Kim, Yeun-Jung (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea. ) , Chung, Soo-Young (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea. ) , Jee, Young-Mee (Division of Hepatitis and Poliovirus, Department of Virology, National Institute of Health, 5 Nokbun Dong, Eunpyung Ku, Seoul, 136-701, Korea. ) , Jeon, Eun-Seok (Department of Medicine, Sunkyunkwan University School of Medicine, Cardiac and Vascular)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 89 , 2006 , 1598-2467 ,

    초록

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  2. [국내논문]   Coxsackievirus B3 H3와 10A1 감염에 의한 마우스 심장 내에서의 유전자 변이 관찰  

    남재환 (가톨릭대학교 생명공학부 분자바이러스연구실 ) , 임병관 (성균관대학교 의과대학 순환기내과 ) , 조영주 (가톨릭대학교 생명공학부 분자바이러스연구실 ) , 김대선 (가톨릭대학교 생명공학부 분자바이러스연구실 ) , 김연정 (가톨릭대학교 생명공학부 분자바이러스연구실 ) , 정수영 (가톨릭대학교 생명공학부 분자바이러스연구실 ) , 지영미 (국립보건연구원 간염 폴리오 연구팀 ) , 전은석 (성균관대학교 의과대학 순환기내과)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 89 - 98 , 2006 , 1598-2467 ,

    초록

    Coxsackievirus B3 (CVB3) is a non-enveloped virus that has a single-stranded RNA genome. CVB3 induces myocarditis, and ultimately, dilated cardiomyopathy. A myocarditis variant of CVB3 (CVB3 H3) and its antibody-escape mutant (CVB3 10A1) were studied previously; H3 was found to induce myocarditis and 10A1 was found to be attenuated in infected mice. Although amino acid residue 165, located in a puff region of VP2, was found to be altered (i.e., the H3 asparagine was altered to aspartate in 10A1), the detailed mechanism of attenuation was not clearly elucidated. Here, DNA microarray technology was used to monitor changes in mRNA levels of infected mouse hearts after CVB3 H3 and 10A1 infection. This tool was used to elucidate the pathogenic mechanisms of viral infection by understanding virus-host interactions. We identified several genes, including protein tyrosine kinases, Ddr2 and Ptk2, as well as Clqb and Crry, involved in complement reactions, which may be involved in these viral processes. Thus, gene profiling can provide an opportunity to understand host immune responses to viral infection for gene therapy and may contribute to the identification of the target gene that is modified during treatment of viral myocarditis.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  3. [국내논문]   Construction of Recombinant DNA with F and HN Genes of Newcastle Disease Virus and Its Immunogenicity  

    Kim, Ji-Young (College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea. ) , Chu, Jiaqi (College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea. ) , Park, Jong-Hyeon (National Veterinary Research and Quarantine Service, Anyang, 430-824, Korea. ) , Seo, Sang-Heui (College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea. ) , Park, Chang-Sik (Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Korea. ) , Kim, Myung-Cheol (College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea. ) , Jun, Moo-Hyung (College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea.)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 99 , 2006 , 1598-2467 ,

    초록

    Coxsackievirus B3 (CVB3) is a non-enveloped virus that has a single-stranded RNA genome. CVB3 induces myocarditis, and ultimately, dilated cardiomyopathy. A myocarditis variant of CVB3 (CVB3 H3) and its antibody-escape mutant (CVB3 10A1) were studied previously; H3 was found to induce myocarditis and 10A1 was found to be attenuated in infected mice. Although amino acid residue 165, located in a puff region of VP2, was found to be altered (i.e., the H3 asparagine was altered to aspartate in 10A1), the detailed mechanism of attenuation was not clearly elucidated. Here, DNA microarray technology was used to monitor changes in mRNA levels of infected mouse hearts after CVB3 H3 and 10A1 infection. This tool was used to elucidate the pathogenic mechanisms of viral infection by understanding virus-host interactions. We identified several genes, including protein tyrosine kinases, Ddr2 and Ptk2, as well as Clqb and Crry, involved in complement reactions, which may be involved in these viral processes. Thus, gene profiling can provide an opportunity to understand host immune responses to viral infection for gene therapy and may contribute to the identification of the target gene that is modified during treatment of viral myocarditis.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  4. [국내논문]   Newcastle Disease Virus의 F와 HN 유전자 재조합 DNA 제조 및 면역원성  

    김지영 (충남대학교 수의과대학 ) , 초가기 (충남대학교 수의과대학 ) , 박종현 (국립수의과검역원 ) , 서상희 (충남대학교 수의과대학 ) , 박창식 (충남대학교 형질전환복제돼지연구센터 ) , 김명철 (충남대학교 수의과대학 ) , 전무형 (충남대학교 수의과대학)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 99 - 107 , 2006 , 1598-2467 ,

    초록

    Recombinant DNA vaccines, based on plasmid vectors expressing an antigen under the control of a strong promotor, have several advantages over traditional vaccines. They have been shown to induce a full spectrum of immune responses for humoral and cellular systems and to secure the higher safety and the simplicity of administration. Thus, establishment of DNA vaccines against Newcastle disease virus (NDV) in poultry has been widely investigated using various virus strains and vector systems. In this study, the F and HN genes of NDV CBP-1 strains isolated from diseased pheasants and attenuated by serial passages in egg embryos were cloned using pSLIA vector and constructed two recombinants of pSLIA-tsF and pSLIA-tsHN. The recombinant plasmids were transfected into COS-7 cell and the expression of HN and F proteins were verified by immunofluorescence, SDS-PAGE and Western blot. The recombinant plasmids were injected intramuscularly and intradermally into C57B/6 mouse and a significant increment of HN and F antibodies was detected by ELISA. According to the results, it was implicative that the recombinant DNA could be utilized for development of recombinant DNA vaccine for NDV.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  5. [국내논문]   Development of Peptide Antibody against Coxsackievirus B3 VP2  

    Chung, Soo-Young (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea. ) , Cho, Young-Joo (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea. ) , Kim, Yeun-Jung (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea. ) , Kim, Dae-Sun (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea. ) , Lee, Heuiran (Department of Micorbiology, University of Ulsan, College of Medicine, Seoul, Korea. ) , Nam, Jae-Hwan (Department of Biotechnology, The Catholic University of Korea, 43-1 Yeokgok Dong, Wonmi-Ku, Bucheon, 420-743, Korea.)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 109 , 2006 , 1598-2467 ,

    초록

    Recombinant DNA vaccines, based on plasmid vectors expressing an antigen under the control of a strong promotor, have several advantages over traditional vaccines. They have been shown to induce a full spectrum of immune responses for humoral and cellular systems and to secure the higher safety and the simplicity of administration. Thus, establishment of DNA vaccines against Newcastle disease virus (NDV) in poultry has been widely investigated using various virus strains and vector systems. In this study, the F and HN genes of NDV CBP-1 strains isolated from diseased pheasants and attenuated by serial passages in egg embryos were cloned using pSLIA vector and constructed two recombinants of pSLIA-tsF and pSLIA-tsHN. The recombinant plasmids were transfected into COS-7 cell and the expression of HN and F proteins were verified by immunofluorescence, SDS-PAGE and Western blot. The recombinant plasmids were injected intramuscularly and intradermally into C57B/6 mouse and a significant increment of HN and F antibodies was detected by ELISA. According to the results, it was implicative that the recombinant DNA could be utilized for development of recombinant DNA vaccine for NDV.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  6. [국내논문]   Coxsackievirus B3의 VP2 부분에 대한 Peptide 항체 개발  

    정수영 (가톨릭대학교 생명공학부 분자바이러스연구실 ) , 조영주 (가톨릭대학교 생명공학부 분자바이러스연구실 ) , 김연정 (가톨릭대학교 생명공학부 분자바이러스연구실 ) , 김대선 (가톨릭대학교 생명공학부 분자바이러스연구실 ) , 이희란 (울산대학교 의과대학 미생물학교실 ) , 남재환 (가톨릭대학교 생명공학부 분자바이러스연구실)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 109 - 117 , 2006 , 1598-2467 ,

    초록

    Coxsackievirus B3 (CVB3) is the nonenveloped virus containing a single-stranded positive-sense RNA as a genome. CVB3 infection can induce acute myocarditis and dilated cardiomypathy. CVB3 of icosahedral symmetry has four capsid proteins called VP1, VP2, VP3, and VP4. Although VP1 is a major antigenic determinant, VP2 is also an important protein for viral physiology, such as maturation cleavage and attenuation. However, VP2 study has been hampered, partly because VP2 antibody is not available. In this study, we developed peptide-based polyclonal VP2 antibody and analyzed its potency by Western blotting analysis and immunofluorescent assay. Purified B3-1 antibody (VP2 peptide antibody developed in here) showed the sensitivity and specificity, similar to VP1 monoclonal antibody which is commercially available. Moreover, this peptide antibody may be useful for double-staining with other antibodies derived from mouse. Therefore, the VP2 antibody may allow us to study CVB assembly and understand VP2 function in depth.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  7. [국내논문]   Screening of Peptide Libraries to Investigate the Substrate Specificity of UL97 Protein Kinase from Human Cytomegalovirus  

    Baek, Moon-Chang (Department of Molecular Medicine, School of Medicine, Kyungpook National University, 101 Dongin-Dong, Jung-Gu, Daegu 700-422, Korea.)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 119 , 2006 , 1598-2467 ,

    초록

    Coxsackievirus B3 (CVB3) is the nonenveloped virus containing a single-stranded positive-sense RNA as a genome. CVB3 infection can induce acute myocarditis and dilated cardiomypathy. CVB3 of icosahedral symmetry has four capsid proteins called VP1, VP2, VP3, and VP4. Although VP1 is a major antigenic determinant, VP2 is also an important protein for viral physiology, such as maturation cleavage and attenuation. However, VP2 study has been hampered, partly because VP2 antibody is not available. In this study, we developed peptide-based polyclonal VP2 antibody and analyzed its potency by Western blotting analysis and immunofluorescent assay. Purified B3-1 antibody (VP2 peptide antibody developed in here) showed the sensitivity and specificity, similar to VP1 monoclonal antibody which is commercially available. Moreover, this peptide antibody may be useful for double-staining with other antibodies derived from mouse. Therefore, the VP2 antibody may allow us to study CVB assembly and understand VP2 function in depth.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  8. [국내논문]   Human Cytomegalovirus의 UL97 단백질 인산화 효소의 기질 특이성 연구를 위한 Peptide Library의 스크리닝  

    백문창 (경북대학교 의과대학 분자의학교실)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 119 - 124 , 2006 , 1598-2467 ,

    초록

    Human cytomegalovirus encodes an unusual protein kinase UL97 which can phosphorylate exogenous substrates, including histone H2B and nucleoside analogs such as ganciclovir. The previous result interestingly showed that the peptides phosphorylated by UL97 have K/R at the 5 positions (P+5) downstream from the pSer. To confirm the importance of the basic residue in the position, we used two peptide libraries, 4S4K (MAXXXXSXXXXKXANNN) and 4S6N (MAXXXXSXXXXXXNNN). The activity of phosphorylation by UL97 was higher in the peptide library 4S4K than 4S6N, suggesting the importance of basic residue at P+5 position. The screening with a peptide library 4S4K showed slight tendencies for N in the P+1 and P+2, M in the P+2, K in the P+4 and P+6 positions and several amino acids in the other positions. This result will give information to develop an optimal peptide for screening a novel UL97 inhibitor.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  9. [국내논문]   Evidence for the Presence of acis-acting Element in the Coding Region of the Japanese Encephalitis Virus Capsid Protein  

    Yun, Sang-Im (Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Korea. ) , Choi, Yu-Jeong (Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Korea. ) , Song, Byung-Hak (Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Korea. ) , Kim, Jeong-Min (Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Korea. ) , Kang, Shien-Young (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea. ) , Lee, Young-Min (Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Korea.)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 125 , 2006 , 1598-2467 ,

    초록

    Human cytomegalovirus encodes an unusual protein kinase UL97 which can phosphorylate exogenous substrates, including histone H2B and nucleoside analogs such as ganciclovir. The previous result interestingly showed that the peptides phosphorylated by UL97 have K/R at the 5 positions (P+5) downstream from the pSer. To confirm the importance of the basic residue in the position, we used two peptide libraries, 4S4K (MAXXXXSXXXXKXANNN) and 4S6N (MAXXXXSXXXXXXNNN). The activity of phosphorylation by UL97 was higher in the peptide library 4S4K than 4S6N, suggesting the importance of basic residue at P+5 position. The screening with a peptide library 4S4K showed slight tendencies for N in the P+1 and P+2, M in the P+2, K in the P+4 and P+6 positions and several amino acids in the other positions. This result will give information to develop an optimal peptide for screening a novel UL97 inhibitor.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  10. [국내논문]   일본뇌염바이러스 C 단백질 코딩 염기부위에 존재하는 시스-작용 유전인자에 대한 실험적 증거  

    윤상임 (충북대학교 의과대학 미생물학교실 기초의학연구소 ) , 최유정 (충북대학교 의과대학 미생물학교실 기초의학연구소 ) , 송병학 (충북대학교 의과대학 미생물학교실 기초의학연구소 ) , 김정민 (충북대학교 의과대학 미생물학교실 기초의학연구소 ) , 강신영 (충북대학교 수의과대학 동물의학연구소 ) , 이영민 (충북대학교 의과대학 미생물학교실 기초의학연구소)
    Journal of bacteriology and virology : JBV v.36 no.2 ,pp. 125 - 132 , 2006 , 1598-2467 ,

    초록

    Japanese encephalitis virus (JEV) is a member of mosquito-borne flaviviruses. To investigate whether there is a cis-acting genetic element in the coding region of the JEV C protein, which is required for viral replication, we generated four mutants by introducing a various size of deletions in each structural protein-coding region, designated as $pJEV/Rep/{\Delta}CC/LUC,\;pJEV/Rep/{\Delta}C/LUC,\;pJEV/Rep/{\Delta}prM/LUC,\;and\;pJEV/Rep/{\Delta}E/LUC$ , of these, all replicons except for $pJEV/Rep/{\Delta}CC/LUC$ were competent in replication. Since $pJEV/Rep/{\Delta}CC/LUC$ is the same as $pJEV/Rep/{\Delta}C/LUC$ except for an additional 5' deletion (nt $132{\sim}201$ ) in the coding region of the C protein, this region appeared to be essential for RNA replication. This is consistent with the proposed cyclization sequence motif in the 5' region of the C gene, which has been recently shown to be required for replication in other mosquito-borne flaviviruses such as DV, YFV, KUN, and WNV. Thus, our results suggest that a cis-acting genetic element in the coding region of the JEV C protein may play an important role in RNA replication. This study will facilitate the current understanding of JEV RNA replication.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지

논문관련 이미지