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Horticulture, Environment, and Biotechnology 15건

  1. [국내논문]   Genome-wide identification and description of MLO family genes in pumpkin (Cucurbita maxima Duch.)   SCIE

    Win, Khin Thanda , Zhang, Chunying , Lee, Sanghyeob
    Horticulture, Environment, and Biotechnology v.59 no.3 ,pp. 397 - 410 , 2018 , 2211-3452 ,

    초록

    The Mildew resistance locus o (Mlo) is a plant-specific gene family that encodes a protein with seven-transmembrane (TM) domains that play an important role in plant resistance to powdery mildew (PM). PM caused by Podosphaera xanthii is a widespread plant disease and represents the major fungal threat for many Cucurbits. The recently reported Cucurbita maxima genome sequence data provides an opportunity to identify and characterize the MLO gene family in this species. A total of twenty genes designated CmaMLO1 to CmaMLO20 have been identified using an in silico cloning method with the MLO gene sequences of Cucumis sativus, Cucumis melo, Citrullus lanatus, and Cucurbita pepo as probes. These CmaMLOs were evenly distributed on 15 of the 20 C. maxima chromosomes without any obvious clustering. Multiple sequence alignment showed that common structural features of MLO gene family, such as TM domains, a calmodulin-binding domain, and 30 important amino acid residues for MLO function, were well conserved. Phylogenetic analysis of the CmaMLO genes and other plant species revealed seven different clades (I through VII); however, clade IV is specific to monocots (rice, barley and wheat). Expression analysis showed that four of the five CmaMLO genes that belonged to clade V were up-regulated in pumpkins infected with P. xanthii. Phylogenetic and expression analysis provided preliminary evidence that these five genes could be susceptibility genes that are important for PM resistance. This study is the first comprehensive report on MLO genes in C. maxima to our knowledge. These findings will facilitate the functional analysis of MLOs related to PM susceptibility and are valuable resources for the development of disease resistance in pumpkin.

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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    Fig. 1 이미지
  2. [국내논문]   De novo transcriptome analysis of an albino mutant Pasphiopedilum pacific shamrock reveals reduced expression of genes related to chloroplast biosynthesis and division   SCIE

    Li, Han , Cao, Hua , Yu, Rong-pei , Miao, Zhen , Wang, Ji-hua , Qu, Su-Ping , Yuan, Qiang , Li, Shen-chong
    Horticulture, Environment, and Biotechnology v.59 no.3 ,pp. 411 - 421 , 2018 , 2211-3452 ,

    초록

    The Mildew resistance locus o (Mlo) is a plant-specific gene family that encodes a protein with seven-transmembrane (TM) domains that play an important role in plant resistance to powdery mildew (PM). PM caused by Podosphaera xanthii is a widespread plant disease and represents the major fungal threat for many Cucurbits. The recently reported Cucurbita maxima genome sequence data provides an opportunity to identify and characterize the MLO gene family in this species. A total of twenty genes designated CmaMLO1 to CmaMLO20 have been identified using an in silico cloning method with the MLO gene sequences of Cucumis sativus, Cucumis melo, Citrullus lanatus, and Cucurbita pepo as probes. These CmaMLOs were evenly distributed on 15 of the 20 C. maxima chromosomes without any obvious clustering. Multiple sequence alignment showed that common structural features of MLO gene family, such as TM domains, a calmodulin-binding domain, and 30 important amino acid residues for MLO function, were well conserved. Phylogenetic analysis of the CmaMLO genes and other plant species revealed seven different clades (I through VII); however, clade IV is specific to monocots (rice, barley and wheat). Expression analysis showed that four of the five CmaMLO genes that belonged to clade V were up-regulated in pumpkins infected with P. xanthii. Phylogenetic and expression analysis provided preliminary evidence that these five genes could be susceptibility genes that are important for PM resistance. This study is the first comprehensive report on MLO genes in C. maxima to our knowledge. These findings will facilitate the functional analysis of MLOs related to PM susceptibility and are valuable resources for the development of disease resistance in pumpkin.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  3. [국내논문]   Allelic variation in Brassica oleracea CIRCADIAN CLOCK ASSOCIATED 1 (BoCCA1) is associated with freezing tolerance   SCIE

    Song, Hayoung , Yi, Hankuil , Han, Ching-Tack , Park, Jong-In , Hur, Yoonkang
    Horticulture, Environment, and Biotechnology v.59 no.3 ,pp. 423 - 434 , 2018 , 2211-3452 ,

    초록

    Freezing tolerance is an important horticultural trait in cabbage (Brassica oleracea). Molecular markers for freezing tolerance are needed for marker-assisted breeding of freezing-tolerant cabbage plants. To develop gene-based molecular markers for freezing-tolerance in cabbage, we focused on CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), a core circadian clock component that affects metabolic pathways and confers cold tolerance by upregulating C-repeat binding factor (CBF) pathway genes. We cloned and analyzed CCA1 genes (BoCCA1s) from seven inbred lines and one cultivar of B. oleracea ssp. capitata. Two types of BoCCA1 alleles were detected: BN106-type (freezing-tolerant; BoCCA1-1) and BN107-type (freezing-susceptible; BoCCA1-2). Numerous insertions/deletions (InDels), simple sequence repeats, and single nucleotide polymorphisms (SNPs) were found in the exons and introns of BoCCA1s from the ATG start codon at the second exon to the TGA stop codon at the eighth exon. Using InDels and SNPs, we designed PCR primer pairs to distinguish the freezing-tolerant lines, and validated these markers using 102 cabbage lines and cultivars. The inbred lines possessed either the BN106-type or BN107-type allele, but most cultivars had both alleles. Freezing-tolerant cabbage plants had BN106-type InDels and/or BN106-type SNPs regardless of the presence of BN107-type InDels and SNPs, and BN106-type SNPs were more widely detected in the freezing-tolerant cabbage plants than BN106-type InDels. The expression patterns of BoCCA1-1 and BoCCA1-2 were similar under normal versus temperature-stressed conditions (low and high temperatures), suggesting a functional difference at the post-transcriptional level. Cabbage breeders should use several markers derived from different genes and independently established inbred lines from different seed companies.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  4. [국내논문]   Expression of anthocyanin biosynthesis-related genes reflects the peel color in purple tomato   SCIE

    Kang, Song-I , Rahim, Md Abdur , Afrin, Khandker Shazia , Jung, Hee-Jeong , Kim, Hoy-Taek , Park, Jong-In , Nou, Ill-Sup
    Horticulture, Environment, and Biotechnology v.59 no.3 ,pp. 435 - 445 , 2018 , 2211-3452 ,

    초록

    Freezing tolerance is an important horticultural trait in cabbage (Brassica oleracea). Molecular markers for freezing tolerance are needed for marker-assisted breeding of freezing-tolerant cabbage plants. To develop gene-based molecular markers for freezing-tolerance in cabbage, we focused on CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), a core circadian clock component that affects metabolic pathways and confers cold tolerance by upregulating C-repeat binding factor (CBF) pathway genes. We cloned and analyzed CCA1 genes (BoCCA1s) from seven inbred lines and one cultivar of B. oleracea ssp. capitata. Two types of BoCCA1 alleles were detected: BN106-type (freezing-tolerant; BoCCA1-1) and BN107-type (freezing-susceptible; BoCCA1-2). Numerous insertions/deletions (InDels), simple sequence repeats, and single nucleotide polymorphisms (SNPs) were found in the exons and introns of BoCCA1s from the ATG start codon at the second exon to the TGA stop codon at the eighth exon. Using InDels and SNPs, we designed PCR primer pairs to distinguish the freezing-tolerant lines, and validated these markers using 102 cabbage lines and cultivars. The inbred lines possessed either the BN106-type or BN107-type allele, but most cultivars had both alleles. Freezing-tolerant cabbage plants had BN106-type InDels and/or BN106-type SNPs regardless of the presence of BN107-type InDels and SNPs, and BN106-type SNPs were more widely detected in the freezing-tolerant cabbage plants than BN106-type InDels. The expression patterns of BoCCA1-1 and BoCCA1-2 were similar under normal versus temperature-stressed conditions (low and high temperatures), suggesting a functional difference at the post-transcriptional level. Cabbage breeders should use several markers derived from different genes and independently established inbred lines from different seed companies.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  5. [국내논문]   Optimization of TILLING system based on capillary electrophoresis for targeted selection of pepper gene mutants   SCIE

    Kang, Han Sol , Kim, Sang Hoon , Lee, Sang Woo , Kim, Se Won , Ryu, Jaihyunk , Kim, Jin-Baek , Yeom, Seon-In , Kang, Si-Yong , Jo, Yeong Deuk
    Horticulture, Environment, and Biotechnology v.59 no.3 ,pp. 447 - 460 , 2018 , 2211-3452 ,

    초록

    Mutation breeding is largely performed by forward and reverse genetics-based approaches. In the latter, Target Induced Local Lesions IN Genomes (TILLING) can be used to screen mutants harboring mutations in a target gene whose function can be predicted with available functional genomic information. In TILLING, it is necessary to use an analytical system for accurate and efficient screening of target gene mutants among several randomly mutagenized lines. Therefore, we optimized a capillary electrophoresis-based TILLING system that can quickly and easily analyze many samples and confirmed its performance by applying it to pepper mutation populations. First, optimal conditions concerning heteroduplex DNA formation, type and concentration of endonucleases, and concentration, pooling depth, and size of PCR amplicons were determined in an analytical system using a capillary electrophoresis apparatus without labeled primers. The results showed that CelII endonuclease (Surveyor (TM)) had higher sensitivity than CelI. While the recommended concentration in the manufacturer's manual was 1x, we obtained higher sensitivity when the amount of PCR product and concentration of the endonucleases were at 180 ng and 0.5x for CelII and 120 ng and 2x for CelI. Meanwhile, the maximum pooling depth for DNA from individuals in mutant populations inversely correlated to PCR amplicon size. The limitation of DNA pooling was 4x for a 1872-bp amplicon, while at least 16x pooling was possible for a 672-bp amplicon. Using this optimized system, TILLING was performed on 2615 individuals from the mutant populations developed in a Korean pepper landrace, "Yuwolcho," to screen lines bearing a mutation in CCS (capsanthin/capsorbin synthase). We were able to select a mutant line with a substituted base pair in the translational start codon of CCS. The capillary electrophoresis-based TILLING system optimized in this study is expected to be useful in practical applications for reverse genetics-based breeding and functional genomics studies.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지

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