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Cell 35건

  1. [해외논문]   Chromatin Accessibility Landscape in Human Early Embryos and Its Association with Evolution   SCI SCIE

    Gao, Lei (CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 100101 Beijing, China ) , Wu, Keliang (Center for Reproductive Medicine, Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Key Laboratory of Reproductive Endocrinology, Shandong University, Ministry of Education, Jinan, 250001 Shandong, China ) , Liu, Zhenbo (CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 100101 Beijing, China ) , Yao, Xuelong (CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 100101 Beijing, China ) , Yuan, Shenli (CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, 100101 Beijing, China ) , Tao, Wenrong (Center for Reproductive Medicine, Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Key Laboratory of Reproductive End) , Yi, Lizhi , Yu, Guanling , Hou, Zhenzhen , Fan, Dongdong , Tian, Yong , Liu, Jianqiao , Chen, Zi-Jiang , Liu, Jiang
    Cell v.173 no.1 ,pp. 248 - 259.e15 , 2018 , 0092-8674 ,

    초록

    Summary The dynamics of the chromatin regulatory landscape during human early embryogenesis remains unknown. Using DNase I hypersensitive site (DHS) sequencing, we report that the chromatin accessibility landscape is gradually established during human early embryogenesis. Interestingly, the DHSs with OCT4 binding motifs are enriched at the timing of zygotic genome activation (ZGA) in humans, but not in mice. Consistently, OCT4 contributes to ZGA in humans, but not in mice. We further find that lower CpG promoters usually establish DHSs at later stages. Similarly, younger genes tend to establish promoter DHSs and are expressed at later embryonic stages, while older genes exhibit these features at earlier stages. Moreover, our data show that human active transposons SVA and HERV-K harbor DHSs and are highly expressed in early embryos, but not in differentiated tissues. In summary, our data provide an evolutionary developmental view for understanding the regulation of gene and transposon expression. Highlights The DHS landscape is gradually established during human embryo development OCT4 contributes to zygotic genome activation in humans, but not in mice Younger genes establish DHS at later stages, and older genes show the opposite trend Human transposons SVA/HERVK harbor DHSs and are specifically expressed in embryos Graphical Abstract [DISPLAY OMISSION]

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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    Fig. 1 이미지
  2. [해외논문]   Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis  

    Savitski, Mikhail M. , Zinn, Nico , Faelth-Savitski, Maria , Poeckel, Daniel , Gade, Stephan , Becher, Isabelle , Muelbaier, Marcel , Wagner, Anne J. , Strohmer, Katrin , Werner, Thilo , Melchert, Stephanie , Petretich, Massimo , Rutkowska, Anna , Vappiani, Johanna , Franken, Holger , Steidel, Michael , Sweetman, Gavain M. , Gilan, Omer , Lam, Enid Y.N. , Dawson, Mark A. , Prinjha, Rab K. , Grandi, Paola , Bergamini, Giovanna , Bantscheff, Marcus
    Cell v.173 no.1 ,pp. 260 - 274.e25 , 2018 , 0092-8674 ,

    초록

    Summary The dynamics of the chromatin regulatory landscape during human early embryogenesis remains unknown. Using DNase I hypersensitive site (DHS) sequencing, we report that the chromatin accessibility landscape is gradually established during human early embryogenesis. Interestingly, the DHSs with OCT4 binding motifs are enriched at the timing of zygotic genome activation (ZGA) in humans, but not in mice. Consistently, OCT4 contributes to ZGA in humans, but not in mice. We further find that lower CpG promoters usually establish DHSs at later stages. Similarly, younger genes tend to establish promoter DHSs and are expressed at later embryonic stages, while older genes exhibit these features at earlier stages. Moreover, our data show that human active transposons SVA and HERV-K harbor DHSs and are highly expressed in early embryos, but not in differentiated tissues. In summary, our data provide an evolutionary developmental view for understanding the regulation of gene and transposon expression. Highlights The DHS landscape is gradually established during human embryo development OCT4 contributes to zygotic genome activation in humans, but not in mice Younger genes establish DHS at later stages, and older genes show the opposite trend Human transposons SVA/HERVK harbor DHSs and are specifically expressed in embryos Graphical Abstract [DISPLAY OMISSION]

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  3. [해외논문]   Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis   SCI SCIE

    Savitski, Mikhail M. (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Zinn, Nico (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Faelth-Savitski, Maria (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Poeckel, Daniel (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Gade, Stephan (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Becher, Isabelle (Genome Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany ) , Muelbaier, Marcel (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Wagner, Anne J. (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Strohmer, Katrin (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Werner, Thilo (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Melchert, Stephanie (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Petretich, Massimo (Cellzome GmbH, GlaxoSmithKline, Meyerhofstrasse 1, 69117 Heidelberg, Germany ) , Rutkowska, Anna (Cellzome GmbH, GlaxoSmithKline, Meyerhofstr) , Vappiani, Johanna , Franken, Holger , Steidel, Michael , Sweetman, Gavain M. , Gilan, Omer , Lam, Enid Y.N. , Dawson, Mark A. , Prinjha, Rab K. , Grandi, Paola , Bergamini, Giovanna , Bantscheff, Marcus
    Cell v.173 no.1 ,pp. 260 - 274.e25 , 2018 , 0092-8674 ,

    초록

    Summary Protein degradation plays important roles in biological processes and is tightly regulated. Further, targeted proteolysis is an emerging research tool and therapeutic strategy. However, proteome-wide technologies to investigate the causes and consequences of protein degradation in biological systems are lacking. We developed “multiplexed proteome dynamics profiling” (mPDP), a mass-spectrometry-based approach combining dynamic-SILAC labeling with isobaric mass tagging for multiplexed analysis of protein degradation and synthesis. In three proof-of-concept studies, we uncover different responses induced by the bromodomain inhibitor JQ1 versus a JQ1 proteolysis targeting chimera; we elucidate distinct modes of action of estrogen receptor modulators; and we comprehensively classify HSP90 clients based on their requirement for HSP90 constitutively or during synthesis, demonstrating that constitutive HSP90 clients have lower thermal stability than non-clients, have higher affinity for the chaperone, vary between cell types, and change upon external stimuli. These findings highlight the potential of mPDP to identify dynamically controlled degradation mechanisms in cellular systems. Highlights Multiplexed proteome dynamics profiling, mPDP, measures changes in proteostasis JQ1-PROTAC degrades a key mRNA export factor and blocks protein synthesis Raloxifene induces TMEM97 degradation dysregulating cholesterol homeostasis Characterization of proteins dependent on HSP90 constitutively or during synthesis Graphical Abstract [DISPLAY OMISSION]

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  4. [해외논문]   The Neuronal Gene Arc Encodes a Repurposed Retrotransposon Gag Protein that Mediates Intercellular RNA Transfer   SCI SCIE

    Pastuzyn, Elissa D. , Day, Cameron E. , Kearns, Rachel B. , Kyrke-Smith, Madeleine , Taibi, Andrew V. , McCormick, John , Yoder, Nathan , Belnap, David M. , Erlendsson, Simon , Morado, Dustin R. , Briggs, John A.G. , Feschotte, Cé , dric , Shepherd, Jason D.
    Cell v.173 no.1 ,pp. 275 - 275 , 2018 , 0092-8674 ,

    초록

    Summary Protein degradation plays important roles in biological processes and is tightly regulated. Further, targeted proteolysis is an emerging research tool and therapeutic strategy. However, proteome-wide technologies to investigate the causes and consequences of protein degradation in biological systems are lacking. We developed “multiplexed proteome dynamics profiling” (mPDP), a mass-spectrometry-based approach combining dynamic-SILAC labeling with isobaric mass tagging for multiplexed analysis of protein degradation and synthesis. In three proof-of-concept studies, we uncover different responses induced by the bromodomain inhibitor JQ1 versus a JQ1 proteolysis targeting chimera; we elucidate distinct modes of action of estrogen receptor modulators; and we comprehensively classify HSP90 clients based on their requirement for HSP90 constitutively or during synthesis, demonstrating that constitutive HSP90 clients have lower thermal stability than non-clients, have higher affinity for the chaperone, vary between cell types, and change upon external stimuli. These findings highlight the potential of mPDP to identify dynamically controlled degradation mechanisms in cellular systems. Highlights Multiplexed proteome dynamics profiling, mPDP, measures changes in proteostasis JQ1-PROTAC degrades a key mRNA export factor and blocks protein synthesis Raloxifene induces TMEM97 degradation dysregulating cholesterol homeostasis Characterization of proteins dependent on HSP90 constitutively or during synthesis Graphical Abstract [DISPLAY OMISSION]

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지
  5. [해외논문]   SnapShot: CGAS-STING Signaling   SCI SCIE

    Galluzzi, Lorenzo (Department of Radiation Oncology, Weill Cornell Medical College, New York, NY, USA ) , Vanpouille-Box, Claire (Department of Radiation Oncology, Weill Cornell Medical College, New York, NY, USA ) , Bakhoum, Samuel F. (Sandra and Edward Meyer Cancer Center, New York, NY, USA ) , Demaria, Sandra (Department of Radiation Oncology, Weill Cornell Medical College, New York, NY, USA)
    Cell v.173 no.1 ,pp. 276 - 276.e1 , 2018 , 0092-8674 ,

    초록

    CGAS responds to cytosolic DNA by initiating a STING-dependent response that ultimately engages innate immune effectors to ensure the preservation of organismal homeostasis.

    원문보기

    원문보기
    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

    이미지

    Fig. 1 이미지

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