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Acta tropica 32건

  1. [해외논문]   Pravastatin and simvastatin inhibit the adhesion, replication and proliferation of Toxoplasma gondii (RH strain) in HeLa cells   SCI SCIE

    Sanfelice, Raquel Arruda (Departamento de Patologia Experimental –) , da Silva, Suelen Santos (Laboratório de Protozoologia Experimental, Universidade Estadual de Londrina, Londrina, PR, Brazil ) , Bosqui, Larissa Rodrigues (Departamento de Patologia Experimental –) , Miranda-Sapla, Milena Menegazzo (Laboratório de Protozoologia Experimental, Universidade Estadual de Londrina, Londrina, PR, Brazil ) , Barbosa, Bellisa Freitas (Departamento de Patologia Experimental –) , Silva, Rafaela José (Laboratório de Protozoologia Experimental, Universidade Estadual de Londrina, Londrina, PR, Brazil ) , (Departamento de Patologia Experimental –) , Ferro, Eloí (Laboratório de Protozoologia Experimental, Universidade Estadual de Londrina, Londrina, PR, Brazil ) , sa A. Vieira (Laboratório de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil ) , Panagio, Luciano Aparecido (Laboratório de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil ) , Navarro, Italmar Teodorico (Laboratório de Histologia e Embri) , Bordignon, Juliano , Conchon-Costa, Ivete , Pavanelli, Wander Rogerio , Almeida, Ricardo Sergio , Costa, Idessania Nazareth
    Acta tropica v.167 ,pp. 208 - 215 , 2017 , 0001-706x ,

    초록

    Abstract The conventional treatment for toxoplasmosis with pyrimethamine and sulfadiazine shows toxic effects to the host, and it is therefore necessary to search for new drugs. Some studies suggest the use of statins, which inhibit cholesterol synthesis in humans and also the initial processes of isoprenoid biosynthesis in the parasite. Thus, the objective of this study was to evaluate the activity of the statins pravastatin and simvastatin in HeLa cells infected in vitro with the RH strain of T. gondii . HeLa cells (1×10 5 ) were infected with T. gondii tachyzoites (5×10 5 ) following two different treatment protocols. In the first protocol, T. gondii tachyzoites were pretreated with pravastatin (50 and 100μg/mL) and simvastatin (1.56 and 3.125μg/mL) for 30min prior to infection. In the second, HeLa cells were first infected (5×10 5 ) with tachyzoites and subsequently treated with pravastatin and simvastatin for 24h at the concentrations noted above. Initially, we evaluated the cytotoxicity of drugs by the MTT assay, number of tachyzoites adhered to cells, number of infected cells, and viability of tachyzoites by trypan blue exclusion. The supernatant of the cell cultures was collected post-treatment for determination of the pattern of Th1/Th2/Th17 cytokines by cytometric bead array. There was no cytotoxicity to HeLa cells with 50 and 100μg/mL pravastatin and 1.56 and 3.125μg/mL simvastatin. There was no change in the viability of tachyzoites that received pretreatment. Regarding the pre- and post-treatment of the cells with pravastatin and simvastatin alone, there was a reduction in adhesion, invasion and proliferation of cells to T. gondii . As for the production of cytokines, we found that IL-6 and IL-17 were significantly reduced in cells infected with T. gondii and treated with pravastatin and simvastatin, when compared to control. Based on these results, we can infer that pravastatin and simvastatin alone possess antiproliferative effects on tachyzoites forms of T. gondii , giving these drugs new therapeutic uses. Highlights Simvastatin and pravastatin evaluated in cells infected with Toxoplasma gondii . Analyzed adhesion, cell infection and proliferation of T. gondii in HeLa cells. Reduction in adhesion, and proliferation of the infection in the treated cells. Simvastatin and pravastatin are alternative targets for the treatment of toxoplasmosis. Graphical abstract [DISPLAY OMISSION]

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    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

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  2. [해외논문]   Mosquito repellents: An insight into the chronological perspectives and novel discoveries   SCI SCIE

    Islam, Johirul (Defence Research Laboratory, Tezpur, Assam 784001, India ) , Zaman, Kamaruz (Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam 786004, India ) , Duarah, Sanjukta (Defence Research Laboratory, Tezpur, Assam 784001, India ) , Raju, Pakalapati Srinivas (Defence Research Laboratory, Tezpur, Assam 784001, India ) , Chattopadhyay, Pronobesh (Defence Research Laboratory, Tezpur, Assam 784001, India)
    Acta tropica v.167 ,pp. 216 - 230 , 2017 , 0001-706x ,

    초록

    Abstract Mosquito being the major medically important arthropod vector; requires utmost attention to reduce the sufferings and economic consequences of those living in the endemic regions. This is only possible by minimising the human-mosquito contact by an absolute preventing measure. However, unfortunately, such absolute measures are yet to be developed despite enormous efforts and huge investments worldwide. In the absence of vaccines for number of mosquito-borne diseases, repellents could be an attractive option for both military personal and civilians to minimise the risk of contacting different mosquito-borne diseases. However, to achieve this golden goal, the detailed knowledge of a particular repellent is must, including its mode of repellency and other relevant informations. Here, in the present article, an effort has been made to convey the best and latest information on repellents in order to enhance the knowledge of scientific community. The review offers an overview on mosquito repellents, the novel discoveries, and areas in need of attention such as novel repellent formulations and their future prospective. Highlights We have prepared an extensive review on mosquito repellents with special emphasize on novel discoveries with insect repelling properties. The novel insect repellents demonstrated promising insect repellent activity. These newly discovered insect repellents will pave the way in the development of safer and better alternatives of existing mosquito repellents.

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    무료다운로드 유료다운로드

    회원님의 원문열람 권한에 따라 열람이 불가능 할 수 있으며 권한이 없는 경우 해당 사이트의 정책에 따라 회원가입 및 유료구매가 필요할 수 있습니다.이동하는 사이트에서의 모든 정보이용은 NDSL과 무관합니다.

    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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