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Biochemical and biophysical research communication...Biochemical and biophysical research communications 49건

  1. [해외논문]   Identification of antigenic peptides from novel renal cancer stem-like cell antigen, DNAJB8   SCI SCIE

    Nishizawa, Satoshi (Department of Urology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-8509, Japan ) , Hirohashi, Yoshihiko (Department of Pathology, Sapporo Medical University School of Medicine, South-1, West-17, Cyuo-ku, Sapporo 060-8556, Japan ) , Kusumoto, Hiroki (Department of Urology, Kinan Hospital, 46-70 Shinjocho, Tanabe, Wakayama 646-8588, Japan ) , Wakamiya, Takahito (Department of Urology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-8509, Japan ) , Iguchi, Takashi (Department of Urology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-8509, Japan ) , Yamashita, Shimpei (Department of Urology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-8509, Japan ) , Iba, Akinori (Department of Urology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-8509, Japan ) , Kikkawa, Kazuro (Department of Urology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakayama 641-8509, Japan ) , Kohjimoto, Yasuo (Department of Urology, Wakayama Medical University School of Medicine, 811-1 Kimiidera, Wakaya) , Torigoe, Toshihiko , Hara, Isao
    Biochemical and biophysical research communications v.494 no.3/4 ,pp. 693 - 699 , 2017 , 0006-291x ,

    초록

    Abstract Objectives To identify antigenic peptides of cancer stem-like cells (CSCs) antigen, DNAJB8, and establish a mouse CSCs-targeting immunotherapy model. Materials and methods To induce DNAJB8-specific immune reaction, we stimulated human CD8 + lymphocytes with antigen-presenting cells pulsed with a cocktail of three candidate HLA-A*24:02 restricted peptides and assessed peptide specific human cytotoxic T lymphocytes (CTLs) induction. One of the antigenic peptides showed identical amino acid sequence as corresponding mouse DNAJB8. We evaluated CTL induction with the peptide immunization in mouse model. Results We confirmed peptide-specific interferon-γ secretions and cytotoxic activities of induced human CTLs. In vivo immunization with the peptide to mice, peptide-specific CTL response could be observed in mouse CD8 + T cells. Furthermore, immunization with the peptide showed significant anti-tumor effects compared with negative controls. Conclusion DNAJB8-derived peptide is a novel candidate for CSCs-targeting immunotherapy, and mouse models can be used to evaluate CSCs-targeting immunotherapy. Highlights We identified an antigenic peptide derived from cancer stem-like cell antigen, DNAJB8. The identified peptide showed identical amino acid sequences as mouse DNAJB8. In vivo immunization with the peptide to mouse showed specific CTL responses.

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  2. [해외논문]   Inhibition of tissue factor signaling in breast tumour xenografts induces widespread changes in the microRNA expression profile   SCI SCIE

    D'Asti, Esterina (McGill University, Research Institute of the McGill University Health Centre, Montreal Childrens' Hospital, Montreal, Quebec, Canada ) , Anderson, G. Mark (Centocor, Inc., Radnor, PA, USA ) , Rak, Janusz (McGill University, Research Institute of the McGill University Health Centre, Montreal Childrens' Hospital, Montreal, Quebec, Canada)
    Biochemical and biophysical research communications v.494 no.3/4 ,pp. 700 - 705 , 2017 , 0006-291x ,

    초록

    Abstract Tissue factor (TF) is a transmembrane receptor for coagulation factor VII/VIIa and is frequently overexpressed by cancer cells. The TF/VIIa complex acts as the main initiator of the clotting cascade in blood and a trigger of intracellular signaling that changes gene expression and the cellular phenotype. However, pathways mediating these changes are still poorly characterized and especially the impact of TF signals on regulatory microRNA (miR) networks in cancer remains unknown. We show that the monoclonal antibody that selectively neutralises the signaling (but not coagulant) function of human TF (CNTO 2559) inhibits progression of MDA-MB-231 breast cancer xenografts in mice and prolongs animal survival. CNTO 2559 blocks FVIIa-induced expression of interleukin 8 (IL-8) by cancer cells without impacting factor Xa (FXa) generation. Notably, acute exposure of MDA-MB-231 tumour xenografts to CNTO 2559 systemic injections triggers wide spread changes in the tumour miR profile including alterations in 75 miRs (55 downregulated) and impacting several miR-regulated and cancer-related pathways. These results suggest that TF signaling in the tumour microenvironment may provoke vast changes in the miR profile of cancer cells, affect disease biology, and reflect tumour interaction with the coagulation system, thereby presenting itself as a possible biomarker. Highlights Clotting protein, tissue factor (TF) mediates intracellular signalling in cancer. Selective inhibition of TF signalling exerts potent anticancer effect in mice. Inhibition of TF signalling changes the tumour miR expression profile in vivo . MiRs altered by blockade of TF signalling share common biological pathways.

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  3. [해외논문]   Galangin enhances TGF-β1-mediated growth inhibition by suppressing phosphorylation of threonine 179 residue in Smad3 linker region   SCI SCIE

    Kwak, Mi-Kyung (Precision Medicine Research Center, Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Kyunggi-do 16229, Republic of Korea ) , Yang, Kyung-Min (Precision Medicine Research Center, Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Kyunggi-do 16229, Republic of Korea ) , Park, Jinah (Precision Medicine Research Center, Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Kyunggi-do 16229, Republic of Korea ) , Lee, Siyoung (Precision Medicine Research Center, Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Kyunggi-do 16229, Republic of Korea ) , Park, Yuna (Precision Medicine Research Center, Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Kyunggi-do 16229, Republic of Korea ) , Hong, Eunji (Precision Medicine Research Center, Advanced Institutes of Convergence Technology, Seoul National University, Suwon, Kyunggi-do 16229, Republic of Korea ) , Sun, Eun Jin (Precision Medicine Research Center, Advanced Institutes of Convergence) , An, Haein , Park, Sujin , Pang, Kyoungwha , Lee, Jihee , Kang, Jin Muk , Kim, Pyunggang , Ooshima, Akira , Kim, Seong-Jin
    Biochemical and biophysical research communications v.494 no.3/4 ,pp. 706 - 713 , 2017 , 0006-291x ,

    초록

    Abstract Smad3 linker phosphorylation is a candidate target for several kinases that play important roles in cancer cell initiation, proliferation and progression. Also, Smad3 is an essential intracellular mediator of TGF-β1-induced transcriptional responses during carcinogenesis. Therefore, it is highly advantageous to identify and develop inhibitors targeting Smad3 linker phosphorylation for the treatment of cancers. Galangin (3,5,7-trihydroxyflavone) has been known to be an active flavonoid showing a cytotoxic effect on several cancer cells. However, the mechanism of action of galangin in various cancers remains unclear, and there has been no report concerning regulation of Smad3 phosphorylation by galangin. In the present study, we show that galangin significantly induced apoptosis and inhibited cell proliferation in the presence of TGF-β1 in both human prostate and pancreatic cancer cell lines. Particularly, galangin effectively inhibits phosphorylation of the Thr-179 site at Smad3 linker region through suppression of CDK4 phosphorylation. Thus, galangin can be a promising candidate as a selective inhibitor to suppress phosphorylation of Smad3 linker region. Highlights Galangin significantly induced apoptosis in the presence of TGF-β1. Galangin inhibits phosphorylation of the Thr-179 site at the Smad3 linker region. Galangin suppresses CDK4-mediated Smad3 linker phosphorylation.

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  4. [해외논문]   Molecular characterization of Pod1 during sex development in Chinese tongue sole (Cynoglossus semilaevis)   SCI SCIE

    Wang, Linna (Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China ) , Zhu, Ying (Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China ) , Xu, Wenteng (Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China ) , Shao, Changwei (Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China ) , Dong, Zhongdian (Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China ) , Li, Hailong (Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yell) , Cui, Zhongkai , Meng, Liang , Guo, Hua , Tian, Yongsheng , Chen, Songlin
    Biochemical and biophysical research communications v.494 no.3/4 ,pp. 714 - 718 , 2017 , 0006-291x ,

    초록

    Abstract Pod1 encodes a Class II bHLH transcription factor involved in the development of a number of tissues such as gonad, spleen, lungs and heart. However, to date, little is known about its function in teleosts. In this study, we cloned and characterized Pod1 gene from Cynoglossus semilaevis . This gene contains three exons and two introns, with the full-length cDNA of 918 nucleotides that encodes a 183 amino acid protein with a conserved bHLH domain. Realtime quantitative PCR revealed that Pod1 was predominantly expressed in the testes of C. semilaevis . In different stages of testes development, Pod1 expression was undetectable up to 120 days after hatching (dah), and then increased at 210 dah and 1 year after hatching (yah). Furthermore, in situ hybridization (ISH) analysis revealed that Pod1 was mainly localized in the germ cells of testes, but was not detected in ovarian cells; which suggested its possible functions in spermatogenesis of C. semilaevis . The methylation profile analysis of Pod1 genomic sequence in the gonads showed that the differences in their putative promoter regions of Pod1 among ovary, male and pseudo-male testes were not obvious. Thus, further research might be needed to evaluate whether Pod1 expression is regulated by epigenetic level. Highlights To date, little is known about the function of Pod1 in teleosts. Pod1 was characterized during sex development in Cynoglossus semilaevis. Pod1 mRNA was expressed mainly in the germ cells of male and pseudo-male testes. The methylation profile differences of Pod1 in testes and ovary were not obvious.

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    NDSL에서는 해당 원문을 복사서비스하고 있습니다. 아래의 원문복사신청 또는 장바구니담기를 통하여 원문복사서비스 이용이 가능합니다.

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  5. [해외논문]   Upregulation of PAG1/Cbp contributes to adipose-derived mesenchymal stem cells promoted tumor progression and chemoresistance in breast cancer   SCI SCIE

    Lu, Yunshu (Laboratory of General Surgery and Department of General Surgery, Xinhua Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China ) , Yang, Yipeng (Laboratory of General Surgery and Department of General Surgery, Xinhua Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China ) , Liu, Yan (Department of Pharmacy, Xinhua Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China ) , Hao, Yajuan (Laboratory of General Surgery and Department of General Surgery, Xinhua Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China ) , Zhang, Yijian (Laboratory of General Surgery and Department of General Surgery, Xinhua Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China ) , Hu, Yunping (Laboratory of General Surgery and Department of General Surgery, Xinhua Hospital, Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China ) , Jiang, Lin (Lab) , Gong, Yurong , Wu, Kejin , Liu, Yingbin
    Biochemical and biophysical research communications v.494 no.3/4 ,pp. 719 - 727 , 2017 , 0006-291x ,

    초록

    Abstract C-terminal Src kinase (Csk)-binding protein (Cbp) is a ubiquitously expressed transmembrane adaptor protein which regulating Src family kinase (SFK) activities. Although SFKs are well known for their involvement in breast cancer, the function of Cbp in breast carcinogenesis upon the adipose-tumor microenvironment has not been investigated. Here, we reported that adipose-derived mesenchymal stem cells (ASCs) induced increased expression of Cbp accompanied by enhanced cell proliferation and chemotherapy resistance in breast cancer cell MCF-7/ADR. Depletion of Cbp in breast cancer cell by RNA interference led to remarkable inhibition of cell proliferation, invasion as well as synergy with adriamycin hydrochloride to suppress the tumor growth. Furthermore, silencing of Cbp concomitantly inhibited the expression of phosphoryl of Src, AKT and mTOR signals. Our study highlights the underlying mechanism of cross interaction between ASCs and breast cancer cells, and indicates that PAG1/Cbp in breast cancer cell may modulate tumor progression and acquired chemoresistance in the ASCs-associated breast cancer microenvironment through Src and AKT/mTOR pathways.

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  6. [해외논문]   shRNA interference of NLRP3 inflammasome alleviate ischemia reperfusion-induced myocardial damage through autophagy activation   SCI SCIE

    Meng, Zhu (Department of Senile Cardiovascular Disease, Qingdao Municipal Hospital, Qingdao, 266011, PR China ) , Song, Mei-Yan (Department of Infectious Diseases, Yantaishan Hospital, Yantai, 264001, PR China ) , Li, Chuan-Fang (Department of Cardiology, Affiliated Hospital of Jining Medical University, Jining, 272029, PR China ) , Zhao, Jia-Qi (Department of Cardiology, Affiliated Hospital of Jining Medical University, Jining, 272029, PR China)
    Biochemical and biophysical research communications v.494 no.3/4 ,pp. 728 - 735 , 2017 , 0006-291x ,

    초록

    Abstract Myocardial ischemia-reperfusion (I/R) injury always occur during the recovery of myocardial blood supply with high morbidity and mortality. Although, various therapeutic schedules were applied in clinic, there are real problems that have to be resolved on curative effect. Nod-like receptor protein 3 (NLRP3) inflammasome has moderation effects on cellular damage and inflammatory reaction after I/R injury. Our research aims to investigate a more effective approach to restrain the activation of NLRP3 inflammasome in treating myocardial I/R injury. Results indicated that cell viability, Bax/Bcl-2 expression were affected hardly by sh-NLRP3 transfection in normal cells. However, the decreased cell viability and increased Bax/Bcl-2 expression level caused by I/R were remarkably suppressed through sh-NLRP3 transfection. Besides that, the reduced levels of pro-autophagy proteins (Beclin1, Agt7, LC3II/LC3I) while enhanced level of anti-autophagy protein (p62) and apoptosis-related proteins (Bax/Bcl-2) were significantly repressed via sh-NLRP3 transfection. Nevertheless, the autophagy inhibitor 3 MA could reverse the results. Moreover, in vivo experiment suggested that NLRP3 was up-regulated in wild type (WT) rats with I/R injury. The expansion of infarct size induced by ischemia was tremendously constricted in NLRP3 knockout (KO) rats. NLRP3 silence had nearly no impact on myocardial enzymes (AST, LDH and CK) expressions, inflammatory factors (TNF-α and IL-1β) expressions and cell apoptosis in rats without I/R injury. Nonetheless, the elevated levels of myocardial enzymes, inflammatory factors and cell apoptosis caused by I/R injury were vastly inhibited in NLRP3 KO rats. Furthermore, NLRP3 KO itself would lead to higher level of pro-autophagy proteins (Beclin1, Agt7, LC3II/LC3I) while lower level of anti-autophagy protein (p62) in vivo . The decreased expressions of pro-autophagy proteins while increased expressions of anti-autophagy protein induced by I/R injury were remarkably suppressed by NLRP3 KO. Taken together, our study indicated that shRNA interference of NLRP3 inflammasome attenuated myocardial I/R injury via autophagy activation. These findings demonstrated that NLRP3 KO may a promising therapy in myocardial I/R injury.

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  7. [해외논문]   Crystal structure and thermodynamic dissection of chitin oligosaccharide binding to the LysM module of chitinase-A from Pteris ryukyuensis   SCI SCIE

    Ohnuma, Takayuki (Department of Advanced Bioscience, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan ) , Taira, Toki (Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Okinawa, Japan ) , Umemoto, Naoyuki (Department of Advanced Bioscience, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan ) , Kitaoku, Yoshihito (Department of Advanced Bioscience, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan ) , Sørlie, Morten (Department of Chemistry, Biotechnology, and Food Science, The Norwegian University of Life Sciences, 1432 Ås, Norway ) , Numata, Tomoyuki (Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan ) , Fukamizo, Tamo (Department of Advanced Bioscience, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan)
    Biochemical and biophysical research communications v.494 no.3/4 ,pp. 736 - 741 , 2017 , 0006-291x ,

    초록

    Abstract We determined the crystal structure of a LysM module from Pteris ryukyuensis chitinase-A ( Pr LysM2) at a resolution of 1.8 A. Structural and binding analysis of Pr LysM2 indicated that this module recognizes chitin oligosaccharides in a shallow groove comprised of five sugar-binding subsites on one side of the molecule. The free energy changes (Δ G r °) for binding of (GlcNAc) 6 , (GlcNAc) 5 , and (GlcNAc) 4 to Pr LysM2 were determined to be −5.4, −5,4 and −4.6 kcal mol −1 , respectively, by ITC. Thermodynamic dissection of the binding energetics of (GlcNAc) 6 revealed that the driving force is the enthalpy change (Δ H r ° = −11.7 ± 0.2 kcal/mol) and the solvation entropy change (− T Δ S solv ° = −5.9 ± 0.6 kcal/mol). This is the first description of thermodynamic signatures of a chitin oligosaccharide binding to a LysM module. Highlights Crystal structure of a LysM module from Pteris ryukyuensis chitinase-A ( Pr LysM2) was determined at a resolution of 1.8 A. LysM module recognizes chitin oligosaccharides in a shallow groove on the molecule. Thermodynamic dissection of the binding energetics of (GlcNAc) 6 to Pr LysM2 was carried out. The binding site comprises of five sugar-binding subsites.

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  8. [해외논문]   Corrigendum to “The suppression of torulene and torularhodin treatment on the growth of PC-3 xenograft prostate tumors” [Biochem. Biophys. Res. Communi. (469/4) (2016) 1146–1152]   SCI SCIE

    Du, Chao (School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu Province, PR China ) , Li, Yingchao (School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu Province, PR China ) , Guo, Yahui (School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu Province, PR China ) , Han, Mei (School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu Province, PR China ) , Zhang, Weiguo (School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu Province, PR China ) , Qian, He (School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu Province, PR China)
    Biochemical and biophysical research communications v.494 no.3/4 ,pp. 742 - 743 , 2017 , 0006-291x ,

    초록

    Abstract We determined the crystal structure of a LysM module from Pteris ryukyuensis chitinase-A ( Pr LysM2) at a resolution of 1.8 A. Structural and binding analysis of Pr LysM2 indicated that this module recognizes chitin oligosaccharides in a shallow groove comprised of five sugar-binding subsites on one side of the molecule. The free energy changes (Δ G r °) for binding of (GlcNAc) 6 , (GlcNAc) 5 , and (GlcNAc) 4 to Pr LysM2 were determined to be −5.4, −5,4 and −4.6 kcal mol −1 , respectively, by ITC. Thermodynamic dissection of the binding energetics of (GlcNAc) 6 revealed that the driving force is the enthalpy change (Δ H r ° = −11.7 ± 0.2 kcal/mol) and the solvation entropy change (− T Δ S solv ° = −5.9 ± 0.6 kcal/mol). This is the first description of thermodynamic signatures of a chitin oligosaccharide binding to a LysM module. Highlights Crystal structure of a LysM module from Pteris ryukyuensis chitinase-A ( Pr LysM2) was determined at a resolution of 1.8 A. LysM module recognizes chitin oligosaccharides in a shallow groove on the molecule. Thermodynamic dissection of the binding energetics of (GlcNAc) 6 to Pr LysM2 was carried out. The binding site comprises of five sugar-binding subsites.

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  9. [해외논문]   Corrigendum to “RNCR3: A regulator of diabetes mellitus-related retinal microvascular dysfunction” [Biochem. Biophys. Res. Commun. 482 (2017) 777–783]   SCI SCIE

    Shan, Kun (Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China ) , Li, Chao-Peng (Huai' an First People's Hospital, Nanjing Medical University, Huai an, Jiangsu, China ) , Liu, Chang (Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China ) , Liu, Xin (Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China ) , Yan, Biao (Research Center, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China)
    Biochemical and biophysical research communications v.494 no.3/4 ,pp. 744 - 744 , 2017 , 0006-291x ,

    초록

    Abstract We determined the crystal structure of a LysM module from Pteris ryukyuensis chitinase-A ( Pr LysM2) at a resolution of 1.8 A. Structural and binding analysis of Pr LysM2 indicated that this module recognizes chitin oligosaccharides in a shallow groove comprised of five sugar-binding subsites on one side of the molecule. The free energy changes (Δ G r °) for binding of (GlcNAc) 6 , (GlcNAc) 5 , and (GlcNAc) 4 to Pr LysM2 were determined to be −5.4, −5,4 and −4.6 kcal mol −1 , respectively, by ITC. Thermodynamic dissection of the binding energetics of (GlcNAc) 6 revealed that the driving force is the enthalpy change (Δ H r ° = −11.7 ± 0.2 kcal/mol) and the solvation entropy change (− T Δ S solv ° = −5.9 ± 0.6 kcal/mol). This is the first description of thermodynamic signatures of a chitin oligosaccharide binding to a LysM module. Highlights Crystal structure of a LysM module from Pteris ryukyuensis chitinase-A ( Pr LysM2) was determined at a resolution of 1.8 A. LysM module recognizes chitin oligosaccharides in a shallow groove on the molecule. Thermodynamic dissection of the binding energetics of (GlcNAc) 6 to Pr LysM2 was carried out. The binding site comprises of five sugar-binding subsites.

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