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BOLETOPSIS LEUCOMELAS-DERIVED LECTIN AND ITS SEPARATION AND PURIFICATION METHOD

  • 발명자 / 주소
    ISEMURA MAMORU
    MUTO HARUHIKO
  • 출원인 / 주소
    SHIZUOKA PREFECTURE
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    PROBLEM TO BE SOLVED: To produce a novel lactin from a foodstuff.SOLUTION: A Boletopsis leucomelas-derived lactin is produced by separating protein from an extract obtained by grinding Boletopsis leucomelas, an edible mushroom, with a buffer solution, causing the resultant lectin to be adsorbed by using affinity chromatography using N,N'-diacetylchitobiose as a ligand, and causing the lectin to be desorbed. A method for producing a Boletopsis leucomelas-derived lectin, which has an activity to inhibit malignant cell proliferation and an activity to lead

    PROBLEM TO BE SOLVED: To produce a novel lactin from a foodstuff.SOLUTION: A Boletopsis leucomelas-derived lactin is produced by separating protein from an extract obtained by grinding Boletopsis leucomelas, an edible mushroom, with a buffer solution, causing the resultant lectin to be adsorbed by using affinity chromatography using N,N'-diacetylchitobiose as a ligand, and causing the lectin to be desorbed. A method for producing a Boletopsis leucomelas-derived lectin, which has an activity to inhibit malignant cell proliferation and an activity to lead apoptosis into malignant cells to kill them and provides marcobiotic effects, by intra-abdominal medication, to a mouse having malignant cells implanted thereinto, is provided.COPYRIGHT: (C)2003,JPO

  • 문헌번호
    JPA2003090246799

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