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벼 야생유전자원의 수량안정성 관련 유전자 탐색 및 이용
Identification and utilization of QTLs underlying yield and yield stability from wild relatives of rice

  • 사업명

    21세기프론티어연구개발사업

  • 과제명

    벼 야생유전자원의 수량안정성 관련유전자 탐색 및 이용

  • 주관연구기관

    충남대학교
    Chungnam National University

  • 연구책임자

    안상낙

  • 참여연구자

    홍성미   원평영   오창식   김봉학   리흥린   뉴엔티항   구홍광   윤여태   윤동범   송미희   김동민   ...  

  • 보고서유형

    2단계보고서

  • 발행국가

    대한민국

  • 언어

    한국어

  • 발행년월

    2007-05

  • 과제시작년도

    2007

  • 주관부처

    과학기술부

  • 사업 관리 기관

    한국과학재단
    Korea Science and Engineering Foundtion

  • 등록번호

    TRKO201000012056

  • 과제고유번호

    1355049376

  • 키워드

    야생벼.잡초성벼.양적형질.분자표지.이입계통.유용유전자.wild rice.weedy rice.QTL.MAS.introgression lines.

  • DB 구축일자

    2013-04-18

  • 초록 


    The objectives of the present study were to develop the introgression line populations based on repeated backcrossing and marker-...

    The objectives of the present study were to develop the introgression line populations based on repeated backcrossing and marker-assisted selection from two RILs between a wild rice relative O. rufipogon and a temperate japonica cultivar, Hwayeongbyeo, and between a japonica weedy rice and a Tongil-type rice to identify trait-improving QTLs derived from the wild and the weedy rice that are associated with improved agronomic performance in the cultivated background, and (2) to isolate QTL/gene based on a high-density map for the target QTLs and understand the characteristic of the theses QTLs in rice. A set of introgression lines are being developed, each containing only one or a few
    chromosomal segments from the W1944 and Hapcheonaengmi3 in the background of Hwayeongbyeo and Milyang23, respectively. Each of the introgression lines is nearly isogenic to Hwayeongbyeo or Milyang23 and together, and these lines provide nearly complete coverage of the W1944 and Hapcheonaengmi3 genomes. For the W1944 ILs, 14 lines were selected from 120 lines and backcrossed to the recurrent parent, Hwayeongbyeo, to produce a series of W1944 introgression lines. A secondary selection was made for BC3F1 and BC3F2 plants based on the SSR genotypes and 126 candidate ILs were made for BC3F3 population. For the Milyang23/Hapcheonaengmi3 cross, 18 lines were selected from 80 lines and backcrossed to the recurrent parent, Milyang 23, to produce a series of Hapcheonaengmi 3 introgression lines. A secondary selection was made for BC1F1 plants based on the SSR genotypes and 47 candidate ILs were developed at BC1F3 generation. The nearly isogenic nature of the introgression lines (ILs) provides a relative advantage over other segregating population in the
    rapid implementation of pyramiding approach through crosses and marker analysis. A set of preliminary 126 ILs (BC3F7) carrying variant introgressed segments from a presumed wild progenitor, O. rufipogon Griff. Acc. W1944. collected from Cailing county, Hunan province, China, in the background of an elite Korea japonica cultivar (O. sativa L.), Hwayeongbyeo, was constructed using the marker assisted selection (MAS) technique. The 126 ILs have different wild rice segments on each chromosome with 100% coverage of O. rufipogon introgressed segments on chromosome 1, while for chromosome 10, the coverage was only 33.3%. The mean number of homozygous and heterozygous donor segments were 3 (ranging 0-7) and 4.7 (ranging 1-14.5), respectively, and 31.6% of introgressed segments had
    sizes of less than 10.5 cM. A total of 57 quantitative trait locus (QTLs) and two loci associated with qualitative variation for pericarp coloration were identified and confirmed using single point analysis. The number of QTLs per trait ranged from 2 to 7. Phenotypic variation associated with each QTL varied from 8.6% to 52.2%, with an average of 17.13%. For 14 (25.9%) of the QTLs detected in this study, the O. rufipogon-derived allele contributed the beneficial agronomic effect despite the overall inferior characteristics of the wild phenotype. Favorable alleles from O. rufipogon were identified for panicle number, panicle length, days to heading, secondary branches, spikelet per panicle, 1000 grain weight, and yield per plant. These ILs have been used to identify the trait of agronomic in different environments, tolerance to low temperature, and grain quality. One major QTL, an8 was detected on chromosome 8 using 120 RILs between the Hwayeongbyeo and O. rufipogon Griff. (Acc. W1944) cross. To fine map an8 as a single Mendelion factor, we chose one RIL, CR105 with the target region on chromosome 8 plus six additional introgressed segments from O. rufipogon on six different chromosomes. Two BC1F1 plants were developed from two successive backcrosses of Hwayeongbyeo to CR105. By selfing of two plants, a total of 62 BC1F2 progenies were obtained. Among them, a single plant with the W1944 segment in the target region on chromosome 8 was selected and advanced to BC1F4 generation. 341 BC1F4 plants were evaluated for agronomic traits and domestication related
    traits including awn length for QTL analysis. One major QTL for awn was detected in the interval flanked by the SSR markers RM23326 and RM256. This QTL explained 63.2% of the total phenotypic variance, and the W1944 allele increased awn length. This result indicated that the awn trait was controlled by a single gene which was designated as awn8. A putative QTL for secondary branch was also detected in the target region. Using substitution mapping with informative recombnants in the target region, the awn8 was further narrowed to the interval RM23326-RM5353, with the distance of about 473kb. Additional backcrosses and selections are also underway to narrow down the target region, and developed NILs would provide the basis for additional fine mapping aimed at cloning the awn gene(s).
    One BC1F7 line from a cross between Milyang23 and Hapcheonaengmi3, was selected based on the genotype. This line, CR1835, possessed Hapcheonaengmi 3 segments on chromosomes 11 responsible for increased cold tolerance at the seedling stage. By two backcrosses to Milyang23 and selfing, a total of 88 BC3F7 progenies were developed. 88 BC1F7 population were evaluated for agronomic traits including seed emergence rate at 15 C. One QTL for seed emergence rate was detected on a region on chromosome 11 flanked by the SSR markers RM167 and RM332. This QTL explained 10.0% of the total phenotypic variation, and the Hapcheonaengmi3 allele improved emergence rate at low temperature. Additional experiments to narrow down the target region associated with the trait is underway.


    야생유전자원과 재배벼 간 (화영/W1944 및 밀양23호/합천앵미3호) RIL 집단을 이용하여작성된 유전자지도에 의거, 야생벼의 염색체단편이 최소로 이입된 동시에 이입 단편들이 야생벼의 전 염색체를 포함하는 염색체단편 이입계통 (i...

    야생유전자원과 재배벼 간 (화영/W1944 및 밀양23호/합천앵미3호) RIL 집단을 이용하여작성된 유전자지도에 의거, 야생벼의 염색체단편이 최소로 이입된 동시에 이입 단편들이 야생벼의 전 염색체를 포함하는 염색체단편 이입계통 (introgression lines; ILs)을 육성하고자 80 계통을 선발하였다. 각각의 선발된 80 계통들에 재배벼를 반복친으로 2회 여교배를 실시하여 재배벼의 유전적 배경에 야생벼의 서로 다른 염색체단편이 이입된 144개 계통을 육성하였다. 이입계통을 평가하고 QTL 분석을 한 결과, 수량성, 수당립수, 1. 2차지경 등 농업 및 진화관련 형질 등에 연관된 총 52개의 QTLs이 탐색되었다. 조합별 이입계통 중에는 반복친에 비해 수량성, 수당립수 등의 형질에서 우수한 계통들이 탐색되었다. 화영벼조합은 흰잎마름병 (K1, K2 및 K3) 검정결과 모든 계통이 저항성 반응을 보였다. 내냉성검정 (춘천출장소 및 운남성) 결과, 반복친에 비해 내냉성이 증진된 계통들이 관찰되었다. W1944 조합의 경우 육성된 근동질계통과 substitution mapping을 이용하여 수당립수, 까락 등에 관여하는 QTL의 고밀도지도 작성을 실시하였다. 염색체 8번에 위치하는 W1944 유래 까락 관련 QTL의 위치를 약450 kb 내외로 좁힐 수 있었다. 합천앵미3호 조합의 경우, 저온출아성 QTL을 염색체11번에서 탐지하였으며 고밀도지도 작성을 위한 연구를 진행하고 있다. 내재해성, 진화관련 특성 등 유전자의 기능 검정을 위해 염색체단편 이입계통을 이용하여 물리지도 작성을 실시하였다. 이들 결과를 SCI급 journal에 발표하였으며 (6편), 목표한 염색체단편 이입계통을 140개 이상 육성하였다. 육성계통의 평가 결과 우수한 3-5 계통에 대한 품종등록 예비시험을 실시하였다.


  • 목차(Contents) 

    1. 표지...1
    2. 제출문...2
    3. 보고서 초록...3
    4. 요약문...4
    5. Summary...9
    6. CONTENTS...12
    7. 목차...13
    8. 제1장 연구개발과제의 개요 ...14
    9. 1. 서언...14
    10. 2. 양적형질 관련 유전자 분석 및 분리...1...
    1. 표지...1
    2. 제출문...2
    3. 보고서 초록...3
    4. 요약문...4
    5. Summary...9
    6. CONTENTS...12
    7. 목차...13
    8. 제1장 연구개발과제의 개요 ...14
    9. 1. 서언...14
    10. 2. 양적형질 관련 유전자 분석 및 분리...14
    11. 3. 금후 연구 방향...21
    12. 제2장 국내외 기술개발 현황 ...23
    13. 제1절 자연변이의 탐색 및 육종적 이용...23
    14. 제2절 염색체단편 이입계통 육석...24
    15. 제3장 연구개발 수행 내용 및 결과 ...26
    16. 제1절 야생 유전자원 유용유전자 탐색 및 염색체단편 이입계통 육성...26
    17. 제2절 QTL 유전자 고밀도 작성 및 기능분석...58
    18. 제4장 목표달성도 및 관련분야에의 기여도 ...78
    19. 제1절 연차별 연구 개발 목표 대비 달성도...78
    20. 제2절 연구평가의 착안점...79
    21. 제3절 관련분야 기술발전에의 기여도...80
    22. 제5장 연구개발결과의 활용계획 ...81
    23. 1. 연구결과의 활용화 방안...81
    24. 제6장 연구개발과정에서 수집한 해외과학기술정보 ...83
    25. 제1절 벼의 순화과정 및 분류...83
    26. 제2절 자연변이의 탐색 및 육종적 이용...85
    27. 제7장 참고문헌 ...88
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